蓝藻信号传导系统研究进展

对编码蓝藻二元系统和真核型蛋白质激酶(STPK)的基因研究做一综述,并对它们在信号传导中的作用模式进行了讨论 蓝藻是地球上最早出现的光合自养原核生物,它们重要的生态地位,不论是作为生物资源而显示对人类有利的一面,还是因为严重发生的产毒水华而对人类社会有害的一面都吸引了越来越多的研究。在长期的生物进化过程中,它们发展了一系列独特的形态和生理代谢机制,使其能在各种不同生境中生长、繁殖和扩增。研究蓝藻信号传导途径为阐明其高度环境适应性提供理论基础

基于微流控的海洋监测技术

In the process of recognizing the laws of the ocean, developing the marine economy, protecting and restoring the marine ecological environment, marine analysis and monitoring are essential technologies. Modern ocean monitoring presents the characteristics of in-situ, on-site, and comprehensive, and requires analysis and monitoring equipment to be small, sensitive, rapid, and automated. Microfluidic chip technology, with its outstanding characteristics of micro channel control, low-cost, and high throughput, combined with advanced chemical analysis methods, has shown excellent prospects in the construction of core sensing components of marine analysis and monitoring equipment. In this review, starting from the manufacture of microfluidic chips and the construction of analysis methods, the typical objects of marine monitoring were classified, involving pH, dissolved oxygen, nutrients, heavy metals, organic pollutants and marine microorganisms. The progress of application research in monitoring was reviewed, and its future development was prospected

Design and application of the structure for preventing rolled edges of adhesive film for indwelling needle fixation (一种防留置针贴膜卷边结构的设计与应用)

This paper introduced a designed structure for preventing rolled edges of adhesive film for indwelling needle fixation. The designed structure has been used to prevent the rolled edges of adhesive film and reduce the waste of adhesive film in indwelling needle fixation. (本文介绍一种防留置针贴膜卷边结构, 该结构调节方便, 可以有效防止留置针贴膜发生卷边, 减少贴膜耗材的使用。

Construction and expression of m etaU0thi0nein mutations and the heavy-m etaladsorption capacity of the engineered bacteria

采用PCR技术和同尾酶手段,构建了猴金属硫蛋白（mMT）α域的串连体,并构建了表达MTα串连体的工程菌,获得了表达二聚串连体（MTα2）、三聚串连体（MTα3）、五聚串连体（MTα5）的工程菌.结果表明,工程菌在0.1mmol·L^-1IPTG诱导3h后表达量最高.Cd^2＋浓度为0.5mmol·L^-1时,对照菌pGEX-2T/BL21生长受到严重胁迫,工程菌表现出较强的抗性,MTαn/BL21（n=2、3、5）生长几乎不受到胁迫;Cd2＋浓度为1.0mmol·L^-1时,MTα5/BL21表现出较强的耐受能力.Cd2＋浓度为0.5mmol·L^-1时,工程菌对镉的富集能力明显高于对照,其中富集能力最强的是MTα3/BL21（76%）.与对照pGEX-2T/BL21相比,MT/BL21对锌吸附能力显著提高,而转化有MTα串连体基因的工程菌对锌的吸附能力没有提高,说明工程菌对Cd2＋的吸附特异性增强.结构预测显示,MTα、MTα2、MTα3的二级结构中α螺旋依次增加,推测突变蛋白的疏水性增强,三维结构稳定.MTα4、MTα5二级结构中,α螺旋数目减少,尤其是MTα5,其α螺旋几乎完全遭到破坏,疏水性大为降低.对基因工程菌吸附镉的实验表明,MTα3/BL21对镉具有较高的特异性和富集容量

Construction and expression of metallothionein mutations and the heavy-metal adsorption capacity of the engineered bacteria

采用PCR技术和同尾酶手段,构建了猴金属硫蛋白（mMT）α域的串连体,并构建了表达MTα串连体的工程菌,获得了表达二聚串连体（MTα2）、三聚串连体（MTα3）、五聚串连体（MTα5）的工程菌.结果表明,工程菌在0.1mmol·L^-1IPTG诱导3h后表达量最高.Cd^2＋浓度为0.5mmol·L^-1时,对照菌pGEX-2T/BL21生长受到严重胁迫,工程菌表现出较强的抗性,MTαn/BL21（n=2、3、5）生长几乎不受到胁迫;Cd2＋浓度为1.0mmol·L^-1时,MTα5/BL21表现出较强的耐受能力.Cd2＋浓度为0.5mmol·L^-1时,工程菌对镉的富集能力明显高于对照,其中富集能力最强的是MTα3/BL21（76%）.与对照pGEX-2T/BL21相比,MT/BL21对锌吸附能力显著提高,而转化有MTα串连体基因的工程菌对锌的吸附能力没有提高,说明工程菌对Cd2＋的吸附特异性增强.结构预测显示,MTα、MTα2、MTα3的二级结构中α螺旋依次增加,推测突变蛋白的疏水性增强,三维结构稳定.MTα4、MTα5二级结构中,α螺旋数目减少,尤其是MTα5,其α螺旋几乎完全遭到破坏,疏水性大为降低.对基因工程菌吸附镉的实验表明,MTα3/BL21对镉具有较高的特异性和富集容量

Practice of quality control circle activities to reduce the errors in rechecking of finished drug products in pharmacy intravenous admixture service (降低静脉用药调配中心成品复核差错发生率的品管圈实践)

Objective To investigate the effect of quality control circle (QCC)activities to reduce the errors in rechecking of finished drug products in pharmacy intravenous admixture service (PIVAS). Methods A QCC team was established to conduct QCC activities. The current situation and factors of errors in rechecking of finished drug products in PIVAS were analyzed. Targeted nursing countermeasures were carried out. Results The rate of errors in rechecking of finished drug product in PIVAS was decreased from 75. 93% to 15. 74%. Conclusion The QCC is effective to reduce the errors in rechecking of finished drug products in PIVAS. It helps to ensure the patient safety and promoting the rational drug use in clinical settings. (目的 探讨品管圈活动降低静脉用药调配中心成品复核差错发生率的效果。方法 成立品管圈, 确立“降低成品复核差错发生率”为主题活动, 进行计划拟定、现况把握、原因分析、运用 PDCA 程序, 拟定对策并组织实施, 分析效果。结果 针对改善重点, 通过品管圈活动, 将成品复核差错发生率由75. 93%降至15. 74%。结论 通过品管圈活动, 科室联合整改, 有效降低了成品复核差错发生率, 提高患者用药和临床合理用药的安全性和有效性。

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies