银染巢式RT-PCR检测胃癌淋巴结微转移方法

以Ｋｅｒａｔｉｎ１９ｃＤＮＡ的套式引物建立了巢式ＲＴ－ＰＣＲ扩增Ｋｅｒａｔｉｎ１９ｍＲＮＡ的体系及ＰＡＧＥ－银染检测扩增产物的方法；对扩增体系进行优化后，分析了扩增特异性及检测敏感性，并对临床样品作了初步检测．结果表明：该扩增体系具有较好的特异性，其敏感性可达１０－６μｇＲＮＡ，即可从１０５个淋巴细胞中检出１个胃癌细胞；对临床样品检测的结果与病理检查结果相符．显示该法具有较好的可靠性

Method of Detecting Gastric Cancer Micrometastases in Lymph Nodes by Nest RT-PCR Amplification of Keration 19 and its Application

[目的]建立RT PCR扩增Keratin19mRNA的方法 ,评价其应用前景。[方法]采用Keratin19cDNA的套式引物建立巢式RT PCR扩增体系 ,以酶切分析及DNA点杂交法鉴定扩增产物的特异性 ,逆转录产物系列稀释法分析检测敏感性 ,并对51例临床胃周淋巴结样品作初步检测。[结果]该扩增体系具有较好的扩增特异性 ,检测敏感性达1pgRNA ,相当于从105 个淋巴细胞中检出1个胃癌细胞 ;对临床样品检测结果显示该法较病理检查法敏感性高。[结论]该扩增体系具有较好的特异性、敏感性和较高的可靠性。福建省卫生厅基金!(编号 :96075);福建省自然科学基金!(F00024)资

超声诱导发芽过程柚子籽油品质的变化规律Quality change of grapefruit seed oil during ultrasonic induced germination

为提高柚子籽油的营养成分含量，开发高品质的安全油脂，以梅州金柚柚子籽为研究对象，探究了超声诱导发芽过程中柚子籽油品质的变化规律。结果表明：随着超声诱导发芽时间的延长，柚子籽的得油率逐渐降低，9 d时柚子籽得油率为（52.18±0.01）%，比未发芽时降低了3.56百分点；发芽不改变柚子籽油脂肪酸组成，油酸含量小幅升高，亚油酸和亚麻酸含量小幅降低；发芽5 d时柚子籽油的酸值（KOH）为（1.19±0.04）mg/g，过氧化值为（1.98±0.02）mmol/kg，均符合植物油国家标准；脂溶性营养物质生育酚、角鲨烯含量先升高后下降，发芽5 d时生育酚含量为（265.29±0.05）μg/g，角鲨烯含量为（36.10±0.01）μg/g，均达到了峰值，与未发芽相比分别增长了3.74倍和7.60倍。因此，短时发芽可提高柚子籽油的营养价值。In order to improve the nutrient content of grapefruit seed oil and develop high-quality safe oil, Meizhou grapefruit seed was taken as the research object, and the change rule of grapefruit seed oil quality in the process of germination was studied. The results showed that the oil yield of grapefruit seed decreased gradually with the prolonging of germination time. After germination for 9 d, the oil yield of grapefruit seed was (52.18±001)%, which was 3.56 percentage points lower than that without germination. The fatty acid composition of grapefruit seed oil unchanged during germination, and the content of oleic acid increased slightly, while both the contents of linoleic acid and linolenic acid decreased slightly. The acid value and peroxide value of grapefruit seed oil after germination for 5 d were (1.19±004) mgKOH/g and (1.98±0.02) mmol/kg, which met the national standards of vegetable oil. The contents of fat-soluble nutrients tocopherol and squalene increased first and then decreased, and the contents of tocopherol and squalene after germination for 5 d were (265.29±0.05) μg/g and (36.10±0.01) μg/g, which reached the maximum value and increased by 3.74 times and 7.60 times compared with ungermination, respectively. Therefore, short-term germination can improve the nutritional value of grapefruit seed oil