Physiologial-Biochemistrial Effects of Scylla paramamosain to Cu2+ or Zn2+ or Interaction of Cu2+ and Zn2+ Stress

运用酶学分析方法和组织学技术，研究了水体中不同浓度Cu2+或Zn2+及Cu2+与Zn2+交互作用胁迫对拟穴青蟹生理生化以及鳃和肝胰腺显微结构的影响，探讨Cu2+或Zn2+及Cu2+与Zn2+交互作用胁迫对拟穴青蟹生理生化的影响及免疫防御机制，以期为其健康养殖水质管理提供理论指导，并为甲壳动物环境免疫学研究积累基础资料。本试验的主要结果和结论如下：1．Cu2+及Cu2+与Zn2+交互作用胁迫对拟穴青蟹血淋巴中THC的影响在Cu2+≤0.1mg·L-1胁迫第1天可诱导拟穴青蟹血淋巴中THC的升高，随着胁迫时间的延长，THC显著下降后回升至对照组水平，在Cu2+≥2.0mg·L-1及Cu2+与Zn2...The effects of Cu2+ or Zn2+ or interaction of Cu2+ and Zn2+ stress on the physiologial-biochemistrial parameters (THC、PO、SOD、GSH-Px、MDA、ACP 、AKP、Ua、UL) and histological structure of gill and hepatopancreas of mud crab Scylla paramamosain were determined by enzyme analysis and histology technique during 9d experimental time. The aim was to clarify the physiologial-biochemistrial effects and mechani...学位：理学硕士院系专业：海洋与环境学院海洋学系_海洋生物学学号：2005130217

基于针灸歌赋探析旴江针灸名家席弘临证经验

针灸歌赋《天元太乙歌》《席弘赋》《长桑君天星秘诀歌》是席弘门徒根据其针灸学术思想编撰而成,是其临证经验的最好写照,因为其形成年代的不同,存在一定的差异性,故笔者拟通过上述3篇歌赋对比研究以挖掘其共同之处,以探析其临证经验,以便于临床推广应用。江西中医药大学校级研究生创新专项资金项目（JZYC12B07

A Technique of Automatic Current Sharing for Parallel Switching Power Supply

为了解决大功率开关电源模块并联运行时均流问题,给出了一种应用于并联开关电源的电流自平衡控制方案。介绍了电源并联系统拓扑结构,分析了并联系统模块间环流、功率调节等关键性问题,设计了采用电流自平衡并联技术构成的恒压电源及恒流电源试验。仿真与试验表明,电源模块输出电压、电流波动分别小于0.83%和0.53%,模组之间电流不平衡度小于3.78%,瞬态响应快、稳定性好。In order to solve the problem of the current sharing of parallel high power supplies,a digital current sharing control technique for parallel modularized switching power supply operation was investigated.The structure of the parallel system was presented,and a series of key problems,such as the circumfluence of paralleled system,characteristics of the power regulation were analyzed.The paper also designed some experiments based on automatic current sharing technique for constant voltage power supply and constant current power supply.Through the simulation and experiments,satisfactory dynamic and static performance of the power supply was obtained.The fluctuation of the voltage and current is less than 0.83% and 0.53% respectively,and the difference between the sub modules is less than 3.78%.What's more,the system has fast transient response and good stability

Effect of Yuhan Nuanwei Cream Acupoints Stiking Therapy on Gastric Mucosa TNF-α,PCNA of Mice with Chronic Atrophic Gastritis

目的:研究御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎(CAG)大鼠胃黏膜肿瘤坏死因子-α(TNF-α)、增殖细胞核抗原(PCNA)表达的影响,探讨御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎癌前病变CAG大鼠胃黏膜损伤修复的作用机制。方法:大鼠随机分为正常组、模型组、御寒暖胃膏贴敷胃经穴组、药物对照组,采用综合干预方法复制慢性萎缩性胃炎癌前病变大鼠模型,肉眼下观察大鼠胃黏膜损伤指数,光镜下观察胃黏膜组织的病理变化,采用酶联免疫分析法测定胃黏膜细胞中TNF-α、PCNA的表达水平。结果:与正常组比较,模型组的大鼠胃黏膜组织病理学检查提示存在腺体的萎缩和一定程度的细胞异型增生,大鼠胃黏膜细胞中TNF-α、PCNA的表达水平升高,差异有统计学意义(P<0.05);与模型组比较,御寒暖胃膏贴敷胃经穴组和药物对照组大鼠胃黏膜组织的病理损伤得到修复,大鼠胃黏膜细胞中TNF-α、PCNA的表达水平降低,差异有统计学意义(P<0.05);结论:御寒暖胃膏贴敷胃经穴可以促进CAG大鼠胃黏膜损伤的修复,该作用可能是通过调节胃黏膜细胞TNF-α、PCNA的表达水平来实验的。Objective:To study the effect of Yuhan Nuanwei cream(YNC)acupoints stiking therapy on gastric mucosa tumor necrosis factor-α(TNF-α) and proliferating cell nuclear antigen(PCNA) expression of mice with chronic atrophic gastritis(CAG),so to discuss the renovation mechanism of YNC on CAG precancerous lesions. Methods:The mice were divided into normal group,model group,YNC group,drug control group. Duplicate mice model with CAG precancerous lesions by the method of comprehensive intervention,observe gastric mucosal lesion index of mice with the naked eyes,observe pathological change of gastric mucosa tissue under light microscope,measure expression level of TNF-α and PCNA in gastric mucosa cells by the method of enzyme-linked immunoassay. Results: Comparing with normal group, mice gastric mucosa histopathological examinations in model group reminded that there exist gland atrophy and some degree of cell atypical hyperplasia,the expression level of TNF-α and PCNA in mice gastric mucosa cell are increased,differences being significant(P<0.05). Comparing with model group,pathological damage of mice gastric mucosa tissue in YNC group and drug control group obtained obvious repair,the expression level of TNF-α and PCNA in mice gastric mucosa cell were dropped, differences being significant(P<0.05).Conclusion:The therapy of YNC acupoints stiking therapy can promote the renovation of CAG mice gastric mucosal lesion. This fuction may be experimented by regulating the expression level of TNF-α and PCNA in mice gastric mucosa cell.深圳市科技研发资金项目(JCYJ20130401105615482

Effect of Salinity Stress on the Activities of ATPase and Phosphatase in the Mud Crab(Scylla serrata)

采用酶学分析的方法研究了盐度(分别为5、15、25、35)胁迫下锯缘青蟹鳃、肌肉和肝胰腺中腺苷三磷酸酶和磷酸酶活性变化。结果表明,随着盐度降低,青蟹鳃中Na+,K+-ATPase、Ca2+,Mg2+-ATPase活性均升高,随着胁迫时间延长,Na+,K+-ATPase活性呈现不同的变化规律,且趋于平缓。青蟹肌肉中ACP活性随着盐度的升高而呈小幅度下降,而肝胰腺中ACP活性则呈小幅度升高,但各组之间差异不显著(P>0.05)。不同胁迫时间同一盐度组青蟹肌肉中的ACP活性有升有降。青蟹肌肉、肝胰腺中AKP活性随着盐度升高而升高;随着胁迫时间延长,同一盐度组青蟹肌肉中AKP活性均呈升高趋势,肝胰腺中AKP活性有升有降。由此可见,盐度对青蟹生理生化影响显著。The mud crab(Scylla serrata) is a kind of important marine culture species in China.Salinity is one of the most important environmental factors which relates closely with the development of S.serrata.The activities of ATPase and phosphatase in gill,muscle and hepatopancreas of S.serrata were determined under different salinities(5,15,25,35) for understanding its physiological-biochemical response to salinity stress.The results showed that the effects of salinity stress on Na+,K+-ATPase activity in the gill of S.serrata were significant(P0.05).The AKP activities in both muscle and hepatopancreas increased with the salinity increasing,and increased with the time duration in the same treatment.Both ACP and AKP activities in hepatopancreas were higher than those in muscle.The changes of AKP activities in hepatopancreas and muscle seemed to be related with Na+,K+-ATPase activities changing in gill under different salinities.These results suggested that the physiological-biochemical response of S.serrata to salinity stress was significant,which could offer some theoretic basis for water quality management of mud crab aquaculture.厦门大学高层次人才引进科研启动项目“锯缘青蟹营养需求及其免疫营养研究”;; 江苏省滩涂生物资源与环境保护重点建设实验室项目(JLCBE05007

艾灸预处理对胃黏膜细胞调控作用的研究进展

本文介绍了近年来艾灸疗法对胃黏膜损伤的预保护作用的实验研究进展,主要讨论了艾灸调控胃黏膜细胞增殖与凋亡相关信号通路对胃黏膜损伤的预保护作用,并在此基上提出目前艾灸在预防胃黏膜损伤相关疾病研究中存在的问题与展望

初诊为抑郁症的老年隐球菌性脑膜炎6例报告

目的:探讨老年隐球菌性脑膜炎发病特点和误诊原因。方法:分析6例初诊为抑郁症的老年隐球菌性脑膜炎患者的临床表现、脑脊液特点、治疗和预后表现,以明确发病特点和误诊原因。结果:6例病例初期均表现为头晕、失眠、食纳减少;后期出现颅内感染相对特异症状:认知障碍5例、头痛3例、肢体无力3例、发热2例。脑脊液隐球菌培养阳性明确诊断,抗真菌治疗有效。6例均好转,但是2例患者遗留明显后遗症:1例患者遗留明显的认知障碍和双下肢活动受限,1例双眼失明,2例确诊时间为2、4周。结论:老年患者患病后临床症状常不典型,当一种疾病不能解释所有症状时,需开拓思路,积极完善相关检查,必要时请相关科室协助诊治,尽量减少误诊。福建省厦门市科技局资助项目(项目编号：3502Z20084019

基于“热证可灸”观察艾灸胃经穴对胃热证大鼠胃黏膜损伤修复作用的影响

目的:观察艾灸对胃热证大鼠胃黏膜组织形态的影响。方法:70只Wistar大鼠按随机数字表法随机分为7组,分别为:正常组、胃热证组、胃热艾灸组、胃热艾灸对照组、胃寒证组、胃寒艾灸组、胃寒艾灸对照组。除正常组外,胃热组用辣椒素和乙醇溶液灌胃,胃寒组用冰水灌胃及凉水泡浴等分别复制胃热证、胃寒证模型。造模后除正常组、胃热证组、胃寒证外,其余分别予艾灸干预12天。结果:与正常组比较,胃热证组大鼠躁动、兴奋,胃寒证组精神不振、踡缩、懒动等,胃热证组和胃寒证组的胃黏膜损伤指数明显升高(P <0. 05),胃黏膜组织病理学观察可见黏膜坏死脱落,溃疡形成,间质出现水肿,细胞间隙增宽明显,炎细胞浸润广泛;与模型组比较,胃热艾灸组可以改善大鼠躁动、兴奋等胃热证的证候,胃寒艾灸组可以改善大鼠精神不振、踡缩、懒动等胃寒证的证候,胃热艾灸组和胃寒艾灸组的胃黏膜损伤指数明显下降(P <0. 05),胃热艾灸组和胃寒艾灸组的胃黏膜组织病理学观察可见胃黏膜浅层有细胞脱落,无明显溃疡形成;胃热艾灸对照组和胃寒艾灸对照组无论在一般行为学的证候方面,还是在胃黏膜损伤指数和组织病理学方面均未见明显变化;胃热证组和胃寒证组大鼠的胃黏膜细胞中NT和Survivin的表达下降,艾灸胃经穴可以上调胃黏膜细胞中NT和Survivin的水平,而艾灸对照组则未见明显变化。结论:艾灸可以促进胃热证大鼠胃黏膜损伤修复,在一定程度上证明\"热证可灸\"具有可行性。广东省自然科学基金,编号:2017A030313748;;\n广东省中医药局中医药科研项目,编号:20191294;;\n深圳市卫生计生委科研项目,编号:201607061;;\n深圳市福田区卫生公益性科研项目,编号:FTWS2016003

御寒暖胃膏穴位贴敷对胃癌前病变大鼠胃黏膜的影响

目的:研究御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎癌前病变大鼠胃黏膜的影响,探讨御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎癌前病变大鼠胃黏膜损伤修复的作用机制。方法:大鼠随机分为正常组、模型组、御寒暖胃膏贴敷胃经穴组、御寒暖胃膏贴敷对照点组、药物对照组,采用综合干预方法复制慢性萎缩性胃炎癌前病变大鼠模型,肉眼下观察大鼠胃黏膜损伤指数,光镜下观察胃黏膜组织的病理变化,彩色多普勒观察胃黏膜的血流量。结果:与模型组比较,御寒暖胃膏贴敷胃经穴组和药物对照组大鼠胃黏膜损伤指数值均显著降低(P0.05),胃黏膜组织的病理损伤未得到明显修复,胃黏膜血流量未见显著升高(P>0.05)。结论:御寒暖胃膏贴敷胃经穴可以促进慢性萎缩性胃炎癌前病变大鼠胃黏膜损伤的修复,增加胃黏膜的血流量,并且存在经脉-脏腑特异相关性。深圳市科技研发资金项目(JCYJ20130401105615482

第十八届美国理论与应用力学大会总结

1会议概况2018年6月5—9日,第18届美国理论与应用力学大会(18th U.S. National Congress of Theoretical and Applied Mechanics, USNCTAM2018)在美国芝加哥召开.本次大会由美国力学国家委员会和中国力学学会联合主办,旨在探讨和交流近四年世界范围内在理论和应用力学领域的基础研究、创新技术的最新进展,吸引了来自世界各地的近千名专家学