目的:研究御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎(CAG)大鼠胃黏膜肿瘤坏死因子-α(TNF-α)、增殖细胞核抗原(PCNA)表达的影响,探讨御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎癌前病变CAG大鼠胃黏膜损伤修复的作用机制。方法:大鼠随机分为正常组、模型组、御寒暖胃膏贴敷胃经穴组、药物对照组,采用综合干预方法复制慢性萎缩性胃炎癌前病变大鼠模型,肉眼下观察大鼠胃黏膜损伤指数,光镜下观察胃黏膜组织的病理变化,采用酶联免疫分析法测定胃黏膜细胞中TNF-α、PCNA的表达水平。结果:与正常组比较,模型组的大鼠胃黏膜组织病理学检查提示存在腺体的萎缩和一定程度的细胞异型增生,大鼠胃黏膜细胞中TNF-α、PCNA的表达水平升高,差异有统计学意义(P<0.05);与模型组比较,御寒暖胃膏贴敷胃经穴组和药物对照组大鼠胃黏膜组织的病理损伤得到修复,大鼠胃黏膜细胞中TNF-α、PCNA的表达水平降低,差异有统计学意义(P<0.05);结论:御寒暖胃膏贴敷胃经穴可以促进CAG大鼠胃黏膜损伤的修复,该作用可能是通过调节胃黏膜细胞TNF-α、PCNA的表达水平来实验的。Objective:To study the effect of Yuhan Nuanwei cream(YNC)acupoints stiking therapy on gastric mucosa tumor necrosis factor-α(TNF-α) and proliferating cell nuclear antigen(PCNA) expression of mice with chronic atrophic gastritis(CAG),so to discuss the renovation mechanism of YNC on CAG precancerous lesions. Methods:The mice were divided into normal group,model group,YNC group,drug control group. Duplicate mice model with CAG precancerous lesions by the method of comprehensive intervention,observe gastric mucosal lesion index of mice with the naked eyes,observe pathological change of gastric mucosa tissue under light microscope,measure expression level of TNF-α and PCNA in gastric mucosa cells by the method of enzyme-linked immunoassay. Results: Comparing with normal group, mice gastric mucosa histopathological examinations in model group reminded that there exist gland atrophy and some degree of cell atypical hyperplasia,the expression level of TNF-α and PCNA in mice gastric mucosa cell are increased,differences being significant(P<0.05). Comparing with model group,pathological damage of mice gastric mucosa tissue in YNC group and drug control group obtained obvious repair,the expression level of TNF-α and PCNA in mice gastric mucosa cell were dropped, differences being significant(P<0.05).Conclusion:The therapy of YNC acupoints stiking therapy can promote the renovation of CAG mice gastric mucosal lesion. This fuction may be experimented by regulating the expression level of TNF-α and PCNA in mice gastric mucosa cell.深圳市科技研发资金项目(JCYJ20130401105615482
