Areviewonstemcell technology and prospects of its application to aquatic ani mal research

广东省自然科学基金资助项目(04011209

有机玻璃表面增强增透的研究

采用溶胶-凝胶体系制备光学塑料(有机玻璃:PMMA)表面增强的有机硅树脂涂层,制得Si-O-Si刚性交联网络涂层,具有良好的耐磨性及附着力,其表面平整、光滑,同时对PMMA基板的透光性具有明显的改善.并通过红外吸收光谱(FT-IR),紫外分光光度计(UV-vis)等手段,对涂层固化过程及各项性能进行表征

Antiviral Effect of Glycyrrhizin on Porcine Respiratory Coronavirus (PRCV)

利用ST细胞研究了甘草酸对猪呼吸道冠状病毒(PRCV)体外复制过程中的抑制作用。通过RT-PCR和Western blot检测发现甘草酸能有效抑制PRCV的体外复制而对ST细胞不引起明显细胞毒作用。抑制效果与甘草酸的剂量成正比关系。PRCV病毒核衣壳蛋白的表达无论在mRNA水平还是在蛋白质水平均随着甘草酸添加量的增加而降低,提示甘草酸是一种有效的抗PRCV药物。The inhibitory effect of glycyrrhizin (GL) on porcine respiratory coronavirus (PRCV) replication was investigated in vitro in ST cells using RT-PCR and Western blot techniques.GL effectively suppressed PRCV replication without causing apparent cell cytotoxicity in ST cells.The inhibitory effect of GL on PRCV was in a dose-dependent manner.The expression levels of both mRNA and protein of the PRCV nucleocapsid protein in ST cells were impeded by the increasing addition of GL,suggesting it is a potential effective antiviral component against PRCV in vitro.This project was supported by a grant from the Bureauof Science and Technology of Fujian Province (2003N083);; agrant from the Bureau of Science and Technology of Xiamen City(3502Z20031054

PET碳酸饮料瓶结晶与流变性能研究

通过偏光透射法研究PET碳酸饮料瓶结晶分布情况,稳态与动态流变法研究PET树脂的流变性能,探讨PET瓶应力开裂的原因.结果表明:瓶身结晶分布不均匀,树脂分子量分布窄、有极高分子量存在、粘性太大易造成应力集中或应力残留,使PET碳酸饮料瓶耐应力开裂能力变差

An Attribute Reduction Method Based on Rough Sets for Dominating Sets of Graph

探讨粗糙集的属性约简和图的支配集问题之间的联系.通过构造信息系统,将粗糙集的属性约简问题与图的支配集问题相联系,从而把图的支配集问题转化为粗糙集的属性约简问题.首先证明图的极小支配集恰是其构造的信息系统的属性约简,然后提出一种基于信息熵的最小支配集算法,最后通过实例验证该算法的可行性和有效性.The relationship between attribute reduction problem in rough sets and dominating set problem in graph is discussed.By constructing an information system,the attribute reduction problem in rough sets is associated with the dominating set problem in graph,so as to transformed the dominating set problem into the attribute reduction problem.Firstly,it is proved that the minimal dominating set of a graph is exactly the attribute reduction of the constructed information system.Then,a minimum dominating set algorithm based on information entropy is proposed.Finally,A practical example illustrates the feasibility and efficiency of the proposed algorithm.国家自然科学基金项目(No.61379021;11301367;11061004)资

Preparation of Morphology-Tuned γ-MnO_2 and Catalytic Performance for the Liquid-Phase Oxidation of Toluene

通过在回流法制备流程中引入CTAb(十六烷基三甲基溴化铵)、PEg6000(聚乙二醇6000)及P123(聚环氧乙烷-聚环氧丙烷-聚环氧乙烷三嵌段共聚物)等表面活性剂对γ-MnO2催化剂进行形貌控制,同时采用X射线衍射(Xrd)、扫描电镜(SEM)、n2吸附(bET)、热重分析(TgA)、O2程序升温脱附(O2-TPd)以及H2程序升温还原(H2-TPr)等技术对不同形貌γ-MnO2的结构、氧脱附及还原性能进行表征,并考察了其在常压和无溶剂条件下甲苯选择性氧化反应体系的催化特性.同时,对于陈化时间对形貌的影响作用进行了考察.结果表明:不同形貌的γ-MnO2的氧化还原特征及催化活性存在显著差异,其中在经PEg6000进行修饰的γ-MnO2中含有较多的阴离子空位及混合价态,因此有助于分子氧在表面的活化,具有较高的表面比活性;而经P123进行表面修饰的γ-MnO2成晶结构规整、比表面积大,对甲苯液相直接氧化反应则表现出最佳的质量比活性,甲苯转化率达18.1%,含氧化合物总选择性为87.4%,其中苯甲酸的选择性达到73.2%.Introducing surfactants including hexadecyltrimethylammonium bromide (CTAB),macrogol 6000 (PEG6000),and poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) triblock copolymer (P123) into the refluxing aqueous crystal nucleus slurry yielded morphology-tuned microcrystalline γ-MnO2.γ-MnO2 and the influence of surfactant modification were investigated by X-ray diffraction (XRD),scanning electron microscopy (SEM),N2 adsorption (BET),thermogravimetry analysis (TGA),O 2 temperature programmed desorption (O2-TPD),and temperature programmed H2 reduction (H 2-TPR).Surfactants led to differences in γ-MnO2 morphology,surface area,oxygen desorption behavior and reducibility.The effect of reflux time on catalyst morphology is discussed.The catalytic performance of γ-MnO2 during the solvent-free atmospheric oxidation of toluene was evaluated.PEG6000 modified γ-MnO2 exhibited the highest catalytic activity judging by surface area because of a greater mixed valency and more anion vacancies.The greatest mass specific activity was obtained for P123 modified γ-MnO2 with the largest surface area.Optimized reaction conditions yielded an 18.1% toluene conversion,and 87.4 and 73.2% total selectivity and selectivity for benzoic acid,respectively.国家重点基础研究发展规划项目(973)(2010CB226903)资助~

Study on the cloning,expression and function of chicken interleukin-2

以RT-PCR法从鸡脾脏细胞中扩增出鸡白细胞介素2编码基因,通过双酶切、连接步骤克隆进原核表达载体pQE30及pGEX-4T-3,构建了鸡白细胞介素2基因的重组原核表达质粒pQE30-chIL-2及pGEX-chIL-2.重组质粒转化大肠杆菌JM109后经诱导表达,分别得到两种表达产物.以SDS-PAGE和Westernblot检测确认表达产物为带6组氨酸标签及GST蛋白的融合鸡白细胞介素2,分子量分别为16 kDa及39.5 kDa.表达蛋白与抗鸡白细胞介素2单抗均有良好的免疫结合性,进一步证实为鸡白细胞介素2.表达产物经过纯化复性后,具有明显促进鸡脾淋巴细胞增殖的作用.The gene encoding chicken interleukin-2(chIL-2) was amplified from chicken spleen cells by RT-PCR.Following double digestion by restriction endonucleases and ligation,chIL-2 was cloned into prokaryotic expression vectors of pQE30 and pGEX-4T-3 to construct recombinants of pQE30-chIL-2 and pGEX-chIL-2,which were consequently transformed into E.coli JM109.Two kinds of recombinant protein were obtained with IPTG induction.The molecular weights of the expressed proteins were 16?kDa and 39.5?kDa on SDS-PAGE and they were regarded as 6His and GST labeled protein(respectively) as checked by Western blot.Both proteins revealed specific reaction to anti-chIL-2 mAb,further confirming that recombinant proteins are chicken interleukin-2.After purification and(renaturation),the 6His-chIL-2 showed biological activity in stimulating chicken lymphocyte proliferation.福建省科技攻关计划重点项目(2003N083);; 厦门市科技攻关计划重点项目(3502Z20031054);; 集美大学科研启动基金(F03003

光学微球腔谐振模式间隔参数的一种简便计算方法

从光学微球腔谐振模式的本征方程出发,提出了一种利用求导法计算谐振模式间尺寸参数(x)间隔的有效方法。在Lam求得的尺寸参数计算公式基础上,依据谐振模式角量子数(l)必须为整数且远远大于1的性质,相邻模式(Δl=1)间的尺寸参数间隔可近似表示为相应尺寸参数的导数,从而可利用求导法计算谐振模式之间的尺寸参数间隔。研究表明,径向量子数i=1的谐振模式的尺寸参数间隔与Mie散射理论求得的精确数值解之间的相对误差在0.05%以内,并且与实验测量数据基本一致。求导法因不需要进行繁琐的级数展开,从而大大简化了计算程序,为光学微球腔的实验研究提供了理论指导

Characterization and application of nucleocapsid protein of porcine reproductive and respiratory syndrome virus expressed in E.coli

从已构建的PRRSV ORF7重组质粒pUCm-T-ORF7中用PCR扩增ORF7基因亚克隆至表达载体pGEX-4T-3,构建重组表达质粒pGEX-4T-3-ORF7并转化大肠杆菌。经SDS-PAGE及Western blotting鉴定,成功表达了谷胱苷肽转移酶(GST)融合的核衣壳蛋白(N),重组N蛋白表达量约为菌体总蛋白的35%,主要以可溶的形式存在,且能形成同源二聚体。重组N蛋白经谷胱苷肽凝胶(glutathione sepharose 4B)亲和层析后得到高度纯化,并将该蛋白作为抗原建立了间接ELISA检测方法。利用该方法对某猪场76份猪血清进行检测并将结果与IDEXX公司ELISA试剂盒检测结果作比较,2种方法的总符合率达93.4%,检测结果之间差异不显著(P>0.05)。结果表明大肠杆菌表达的重组GST融合N蛋白具有良好的抗原性,因而有望利用该重组蛋白开发为试剂盒应用于临床PRRSV抗体的检测。Porcine reproductive and respiratory syndrome virus(PRRSV) FJ-1 was a newly identified virus isolate in Fujian province.The ORF7 gene of FJ-1 was amplified by RT-PCR and cloned into vector pUCm-T,then subcloned into expression vector of pGEX-4T-3.The recombinant GST-tagged nucleocapsid protein(rN) was expressed in E.coli and the molecular weight was approximately 38 000 as identified by SDS-PAGE and Western blotting.Expression level of the rN protein was approximately 35% of the total bacterial protein and mostly soluble.The rN protein was purified to homogeneity using GST affinity chromatography.Analysis of the rN protein under nonreducing conditions revealed that similar to native protein,the rN protein also possesses homo-dimerization property.An indirect enzyme-linked immunosorbent assay(ELISA) for detecting PRRSV antibody was developed using the purified rN protein as antigen.76 serum samples were detected by the method and the result was compared with that using IDEXX PRRS HerdChek ELISA kit.An identity of 93.4% was revealed between the two ELISA kits and no significant difference(P>0.05) was detected.The data indicate the rN protein has the potential usefulness for detection of the PRRSV antibodies.福建省科技攻关计划重点资助项目(2003N083);; 厦门市科技攻关计划重点资助项目(3502Z20031054