沙眼衣原体的PCR检测

沙眼衣原体(CHlAMydIATrACHOMATIS,C.T)可引起眼部感染,还可引起尿道炎、宫颈炎、子宫内膜炎、输卵管炎、附睾炎、直肠炎等多种疾病。在欧美国家,C.T在泌尿生殖道感染中的严重性已超过淋球菌而成为性传播疾病的主要病原体。在国内,对C.T类疾病的许多情况尚不清楚,据部分地区调查的结果表明,在解脲脲原体(urEAPlASMAurEAlyTICuM),人型支原体(MyCOPlASMAHOMInIS),沙眼衣原体三种人类泌尿生殖道感染的常见病原体中C.T抗体的阳性率是最高的。广州地区性病沙眼衣原体的血清型分析结果与国外报道的基本相似。感染衣原体的孕妇,产下的婴儿中有50~70%将成为带菌者。目前,C.T的实验室诊断方法主要有:细胞培养法、直接免疫荧光法(dfA)、微量免疫荧光法(MIf)、酶联免疫吸附试验(ElISA)、dnA探针杂交法和PCr法等,其中以PCr法简便快速,具有高度的敏感性和特异件

Magnetic resonance image reconstruction using trained geometric directions in 2D redundant wavelets domain and non-convex optimization

Reducing scanning time is significantly important for MRI. Compressed sensing has shown promising results by undersampling the k-space data to speed up imaging. Sparsity of an image plays an important role in compressed sensing MRI to reduce the image artifacts. Recently, the method of patch-based directional wavelets (PBDW) which trains geometric directions from undersampled data has been proposed. It has better performance in preserving image edges than conventional sparsifying transforms. However, obvious artifacts are presented in the smooth region when the data are highly undersampled. In addition, the original PBDW-based method does not hold obvious improvement for radial and fully 2D random sampling patterns. In this paper, the PBDW-based MRI reconstruction is improved from two aspects: 1) An efficient non-convex minimization algorithm is modified to enhance image quality; 2) PBDW are extended into shift-invariant discrete wavelet domain to enhance the ability of transform on sparsifying piecewise smooth image features. Numerical simulation results on vivo magnetic resonance images demonstrate that the proposed method outperforms the original PBDW in terms of removing artifacts and preserving edges

新成员Bak基因及Bcl-2家族

bCl-2蛋白家族在细胞编程死亡的调控中起重要的作用。本文综述了bCl-2家族新成员bAk的特点及其生物学活性,并就bAk及bCl-2家族成员对细胞死亡调控机制进行了初步探讨

Cloning and Functional Expression of CPP32 cDNA in E.coli.

CPP32在细胞编程死亡调控中起重要的作用.利用CPP32专一性引物，用rTPCr方法从人鼻咽癌CnE细胞中扩增出约830bP的片段，经序列分析证明与已发表的CPP32序列完全一致.将扩增出的编码人全长CPP32的CdnA片段克隆入PgEX2T中，转化大肠杆菌dH5α.转化菌经诱导表达出较高含量的gSTCPP32融合蛋白.进一步研究显示，细菌中表达的CPP32蛋白能自我切割，而且能裂解体外翻译的PArP，从而证明其具有生物活性.CPP32 has been recently reported to be involved in the early process of programmed cell death.To Further study CPP32 and its regulation in the cell, a 830 bp cDNA was cloned by RTPCR From CNE cells encoding the Full length human CPP32 protein and high level expression was achieved in E.coli by using GST expression system.The results showed that the bacterially expressed CPP32 protein is autocleaved and capable of cleaving in vitrotranslated PARP, thus is Fully Functional.国家自然科学基

Cloning and Expression of Bcl0-X_L and Bcl-X_S cDNA

利用一对带有TAT在序列的bCl-X专一性引物从bEAS-2b细胞中扩增出比bCl-Xl和bCl-XSCdnA，并将它们克隆入PgEX-5X-3载体进行表达。用IPTg诱导表达的融合蛋白占菌体蛋白总量的30%，经谷胱甘肽-SEPHArOSE亲和层析，蛋白纯度达90%。这些结果为进一步研究bCl-X在编程性细胞死亡中的作用奠定了基础。Utilizing tat-contained bcl-x-speciFic primers designed according to published sequences, bcl-XLand bclxs cDNA were ampliFided From transFormed human airway epidelial cell line.The cDNA Fragments were then cloned into pGEX-5X-3 vector and transFormed into E.coli DH5a.The E.coli transFormed by recombinant pGEX-5X-3 was induced with IPTG to express Fusion proteins.PuriFied Fusion proteins GSTTAT-BCL-Xs and GST-TAT-BCL-XL were got by the method of aFFinity chromatography.国家自然科学基

晚熟酿酒葡萄新品种‘新北醇’

中国科学院植物研究所2004年筛选出‘北醇’芽变株系(‘北醇9号’),2005年开始进行重要的生物学以及植物学特性观测,同时开展了单株酿酒比较试验。2010年开始开展区域试验,其结实性能强,果实可溶性固形物明显高于‘北醇’,苹果酸与酒石酸含量均明显低于‘北醇’,总酸不到其70%,酿酒品质优良。2013年定名为‘新北醇’,并通过北京市林木品种审定委员会审定

优质抗寒抗病酿酒葡萄新品种‘北玺’

葡萄新品种‘北玺’由‘玫瑰香’与山葡萄杂交育成。浆果在北京地区9月底成熟。果粒近圆或椭圆形,紫黑或蓝黑色。平均单穗质量137.9 g,单粒质量2.24 g。可溶性固形物含量23.8%,可滴定酸含量0.52%,出汁率67.4%。抗寒和抗病能力强。酿制的葡萄酒颜色深宝石红,入口柔和、协调,酒体厚实,回味悠长

酿酒葡萄新品种‘北馨’

中国葡萄主产区冬季埋土防寒,生长季喷施化学农药,加大了生产成本与生产风险(李文超和王振平,2012)。选用适合中国气候条件的高抗逆的酿造品种是中国葡萄酒产业目前急需解决的问题之一(刘玺华等,2013;张振文等,2013)。‘北馨’是山葡萄与欧亚种杂交后代。2004年选为优良单株,2007年开始在北京海淀、朝阳、大兴、延庆及天津静海等地进行区域试验,结果表明其抗寒性强,在北京、天津等地冬季不埋土防寒可以安全越冬,生长季病害较少,结实性强,稳产性优于‘北玫’,酿酒品质优良。2013年12月通过北京市林木品种审定委员会的审定,定名为‘北馨’

晚熟酿酒葡萄新品种‘新北醇’

酿酒葡萄新品种‘新北醇’由‘北醇’芽变选种而来。在北京地区9月底成熟。果粒近圆或椭圆形,紫黑色,果粒质量2.27 g,果穗质量178.7 g,果实可溶性固形物含量23.8%,可滴定酸含量0.57%,出汁率66.7%。早果性及丰产性强。抗寒、抗病能力强。酿成的酒宝石红色,香气清新,具有荔枝和树莓的香气,入口柔顺,酒体活泼,回味甜感明显,酸度感较低

酿酒葡萄新品种‘北馨’

‘北馨’系山葡萄与欧亚种品种杂交而来的酿酒葡萄新品种。果粒近圆或椭圆形,紫黑色,单粒质量3.62 g,单穗质量155.5 g,可溶性固形物含量22.4%,可滴定酸含量0.64%,出汁率67.9%。早果性及丰产性强。抗寒、抗病能力强。酿成的酒呈鲜亮的宝石红色,香气清新,具有玫瑰香香气,入口甜美,酒体平衡,口感协调