利用一对带有TAT在序列的bCl-X专一性引物从bEAS-2b细胞中扩增出比bCl-Xl和bCl-XSCdnA，并将它们克隆入PgEX-5X-3载体进行表达。用IPTg诱导表达的融合蛋白占菌体蛋白总量的30%，经谷胱甘肽-SEPHArOSE亲和层析，蛋白纯度达90%。这些结果为进一步研究bCl-X在编程性细胞死亡中的作用奠定了基础。Utilizing tat-contained bcl-x-speciFic primers designed according to published sequences, bcl-XLand bclxs cDNA were ampliFided From transFormed human airway epidelial cell line.The cDNA Fragments were then cloned into pGEX-5X-3 vector and transFormed into E.coli DH5a.The E.coli transFormed by recombinant pGEX-5X-3 was induced with IPTG to express Fusion proteins.PuriFied Fusion proteins GSTTAT-BCL-Xs and GST-TAT-BCL-XL were got by the method of aFFinity chromatography.国家自然科学基
