19 research outputs found
银杏内酯B对于实验性脑出血后神经保护作用研究
目的探讨银杏内酯B对于脑出血后脑水肿、脑细胞凋亡及脑代谢的影响,从多角度进一步探讨银杏内酯B的神经保护机制。方法将50只Sprague-Dawley(SD)大鼠随机分为干预组(n=21)、对照组(n=21)、假手术组(n=8),采用Ⅶ型细菌胶原酶构建大鼠实验性脑出血模型,在脑出血造模后第3天行脑含水量检测;在第3天采用TUNEL法行细胞凋亡检测;在造模成功后,分别在第1天、第3天、第7天,对3组大鼠行氢质子磁共振波谱成像(~1H-MRS),并对图像进行处理及定量分析。结果脑组织含水量对照组[(82.52±0.86)%]与干预组[(81.45±0.54)%]比较,具有显著性差异(P0.05)。结论银杏内酯B可以通过降低脑组织含水量、减少细胞凋亡、改善脑代谢等多途径发挥神经保护作用。厦门市科技局科技惠民计划(项目编号:3502Z20164036
磁共振波谱成像观察银杏内酯B对于脑出血后脑代谢的影响
目的:采用磁共振波谱成像(magnetic resonance spectroscopy,MRS)的方法,研究银杏内酯B对于脑出血后脑代谢的影响,从分子影像角度进一步探讨银杏内酯B的神经保护机制。方法:将20只SD大鼠随机分为干预组(n=7)、对照组(n=7)、假手术组(n=6),采用Ⅶ型细菌胶原酶构建大鼠实验性脑出血模型,在造模成功后,分别在第1天、第3天、第7天,对3组大鼠行氢质子磁共振波谱成像(1H-MRS),并对图像进行处理及定量分析。结果:1H-MR检测结果表明,银杏内酯B3d亚组和7d亚组的N一乙酰天门冬氨酸较对照组明显升高,乳酸明显降低,而胆碱复合物、肌酸差异无统计学意义(P>0.05)。结论:H–MRS能够从分子影像角度分析银杏内酯B对于脑出血后脑代谢的影响,从而揭示银杏内酯B可以通过影响脑代谢发挥神经保护作用。厦门市科技局科技惠民计划,项目编号3502Z2016403
基于慕课的医学生物化学实验教学方法探索
慕课是大规模网络开放课程的简称,在慕课平台上,大学名师的精彩讲授、学生的自主学习体验、师生形式多样的互动过程等被完整系统地实现。针对生物化学课程及现代化教学的特点,将慕课资源应用到教学的各个环节中,这就要求教师在生物化学课程慕课的录制过程中充分利用视频教学的特点,有针对性地开展教学。文章重点探讨了慕课在生物化学课程教学中的应用及录制时的特点,以便教师合理设置课程内容。实践证明,这种线上与线下教学模式的探索提高了学生的学习兴趣,为生物化学课程改革提供了思路。福建省教育厅中青年教师教育科研基金资助项目(JZ160069
Design and practice of comprehensive experiments based on hemoglobin
为不断完善医学生物化学与分子生物学实验课程,实现培养创新性人才的教学目标,在本科生课程中设计以血红蛋白为实验对象,通过蛋白提取、层析纯化、SDS-PAGE电泳分离鉴定及血红蛋白与高铁血红蛋白分子吸收光谱的绘制等内容的医学生物化学综合性实验。教学实践结果表明:该实验具有可行性,学生完成情况良好;适合本科生系统完整地学习蛋白提取、纯化、鉴定及功能的实验方法,具有很强的综合性,获得良好的教学效果。To consummate the construction of medical biochemistry experimental course and cultivate of innovative talents,this study takes the hemoglobin as experimental subject to design a set of integrated biochemical experiments,including extraction,gel chromatography purification,SDS-PAGE separation and acquirement of absorption spectra.The teaching practice results show that the designed experiments are feasible and the teaching effect is encouraging.The comprehensive experiments are suitable for the undergraduates to study the experimental methods on protein purification and identification.福建省科技重点项目(项目编号:2012Y0081
效应物对牛肠激酶活性的影响
肠激酶(EK,EC3.4.21.9)是一种在基因工程产品中广泛应用的工具酶.以小分子荧光物质甘氨酰-天冬氨酰-天冬氨酰-天冬氨酰-天冬氨酰-赖氨酰-β-萘胺(Gly-Asp-Asp-Asp-Asp-Lys-β-naphthylamide,GD4K-β-naphthylamide)为底物,采用荧光跟踪法研究了不同氨基酸、几种常用有机溶剂、EDTA、DTT等对牛肠激酶(BEK)活力的影响.结果表明:L-赖氨酸(L-Lys)、丙酮、EDTA、DTT对该酶的活性有较强的抑制作用,IC50分别约为25,50,50和120mmol/L.进一步研究了L-Lys与丙酮对BEK活力的抑制机理以及抑制类型,结果表明:L-Lys对该酶的抑制机理为可逆抑制,其抑制类型为竞争型抑制类型,其抑制常数KI为12.02mmol/L.丙酮对BEK的抑制类型表现为不可逆抑制
1,3-取代吲唑类低氧诱导因子l抑制剂的设计合成及其抗肝癌活性
低氧诱导因子l(HIF-1)与肿瘤细胞的生长、侵袭和耐药密切相关,在肿瘤细胞内HIF-1高度表达,因此新型的HIF-1抑制剂可作为潜在的抗肿瘤药物。本文合成了9个1,3-取代吲唑衍生物。通过蛋白质印迹(Western Blot)法及实时定量荧光PCR(Real time-PCR)等方法检测了其对HIF-1及其靶基因血管内皮生长因子(VEGF)表达水平的影响,并以3-(5’-羟甲基-2’-呋喃基)-1-苯甲基吲唑(YC-1)为阳性对照药物初步评价了其体外抗肝癌细胞增殖的生物活性。实验发现化合物7b可显著抑制HIF-1及其下游靶基因VEGF的表达,且体外抗肝癌增殖生物活性优于YC-1,半抑制浓度(IC50)值为10.37μmol/L。研究结果表明,3-(5’-羟甲基-2’-呋喃)-1-(1″-对甲苯磺酰基)吲唑具有靶向抑制HIF及良好的抗肝癌活性作用。福建省科技厅项目(2015Y0081,2015J01350);;厦门大学大学生创新创业训练计划项目(2016X0644,20720152005)~
慕课对医学生物化学实验教学改革的启示
慕课是大规模开放式在线课程,是信息时代的新型教学模式。与传统实验教学相比,慕课具有教学理念先进、教学内容合理、教学设计科学、评价方式灵活等优势。医学生物化学实验教学需吸收和借鉴慕课的优点,转变教学理念,优化教学内容,改进教学设计,创新评价方法,提高实验教学质量。福建省教育厅中青年教师教育科研项目(JZ160069
The Nature,Characteristics and Significance of Research of Textbook Language
本文将教材语言作为一个独立的研究领域提出,从概念、性质、特点以及研究意义几个方面来探讨其研究的可能性。对教材语言可能出现的领域、相关研究文献进行了梳理,并对教材语言作了学科属性的定位。随着语言教学和教育目标的不断变化发展,教材语言越来越成为一个不容忽视的重要问题。对它的研究将牵涉到许多不同的领域。The main thrust of this paper is to propose Textbook Language as an independent research area in terms of its concept, nature, characteristics and significance for research. Such issues like potential research topics, main research literature, and the nature of Textbook Language are discussed. Following the development and change of objectives of language teaching and education, Textbook Language becomes an increasingly important topic. Its research involves various fields
The PI3K/Akt and ERK1/2 signaling pathways mediate the erythropoietin-modulated calcium influx in kainic acid-induced epilepsy
Natural Science Research Foundation of China [81 100842, 81171117]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry [44]; Natural Science Foundation of Fujian Province of China [2012J01418]; Basic Foundation of Government [ZK1014]Erythropoietin (EPO) suppresses epileptogenesis and limits the neuronal damage associated with recurrent seizures, but the neurocellular mechanism is unclear. Dysregulation of intracellular calcium homeostasis is a key pathogenic event leading to the progression of epileptic activity, suggesting that EPO may suppress seizures by stabilizing intracellular calcium. In this study, we examined the effects of EPO on voltage-gated Ca2+ influx in cultured rat hippocampal neurons and population spike (PS) amplitude in kainic acid (KA)-induced rats and the mechanisms responsible. KA injection markedly increased EPO and EPO receptor expression and the amplitude of PS in the hippocampal CA3 region, evoked by perforant pathway stimulation. Intracerebroventricular injection of exogenous rat recombinant EPO reversed KA-induced PS amplitude in the hippocampal CA3 region. Similarly, rat recombinant EPO pretreatment attenuates the increased voltage-gated calcium current's (I-Ca) amplitude and density induced by KA in cultured hippocampal neurons. In contrast, transient transfection of rat EPO small interfering RNS (siRNA) further enhanced I-Ca amplitude and density in the presence of KA, whereas a scrambled control siRNA had no effect. Further, EPO activates the PI3K and ERK1/2 pathways in cultured hippocampal neurons, and the PI3K/Akt inhibitor LY294002 and ERK1/2 inhibitor U0126 both blocked, at least in part, the suppressive effect of exogenous EPO on KA-induced calcium currents. This study indicates that both endogenous and exogenous EPO decrease KA-sensitive calcium influx and concomitant hyperexcitability in hippocampal neurons. The results also demonstrate that the PI3K/Akt and ERK1/2 signaling pathways mediate the EPO-modulated calcium influx in KA-induced epilepsy. NeuroReport 24:335-341 (C) 2013 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins
海人酸活化的小胶质细胞H_2O_2、NO的分泌对海马神经元谷氨酸含量的影响
目的:初步探讨海人酸(KA)活化培养的新生SD大鼠小胶质细胞(MG)及其分泌的一氧化氮/过氧化氢(NO/H2O2)、海马神经元、谷氨酸(Glu)之间的相互关系。方法:以KA作为MG的激活剂,以一氧化氮合成酶(NOS)的抑制剂氨基胍(AG)或过氧化氢的水解酶(CAT)作为工具药,先检测原代分离纯化培养的MG在KA激活后及工具药作用后各组条件培养液中NO和H2O2含量的变化,再用各组小胶质细胞条件培养液孵育离体海马神经元,观察其对神经元内Glu表达的影响。结果:KA作用后各组条件培养液中NO和H2O2含量明显升高,经AG或CAT处理后各组条件培养液中NO和H2O2含量显著降低;海马神经元的Glu免疫反应性KA组较对照组明显增强,在AG+KA组和CAT+KA组较KA组明显减弱。结论:活化的MG可能通过氧化损伤机制作为参与癫痫发作一个重要环节,提示采取抑制小胶质细胞活化、减少氧化损伤也可能是癫痫防治研究的一个重要方向