Progesterone regulating glutathione S-transferase Omega-1 expression in the mouse uterine luminal and glandular epithelium during preimplantation period

目的研究谷胱甘肽S-转移酶Omega-1(Gsto 1)在小鼠胚胎着床过程中的表达和孕酮的调节。方法105只CD1小鼠,分为正常妊娠模型和类固醇激素处理模型。正常妊娠模型中,收集妊娠第1~第5天子宫,采用Real-time PCR、原位杂交和Western blotting 3种方法检测Gsto1的表达变化;类固醇激素处理模型均采用卵巢切除2周后的小鼠,又分为雌孕激素处理组、孕酮处理不同时间组和孕酮受体拮抗剂Ru486处理组,所有组中的对照均用芝麻油处理。雌孕激素处理组中,收集芝麻油、雌激素、孕酮、雌激素加孕酮分别处理12h后的子宫;孕酮处理不同时间组中,收集芝麻油和孕酮分别处理1、3、12、24 h后的子宫;Ru486处理组中,收集芝麻油、Ru486、孕酮、Ru486加孕酮分别处理12 h后的子宫。类固醇激素处理模型使用Real-time PCR和Western blotting两种方法检测Gsto1的表达变化。结果 Gsto1主要在妊娠第1~4天的子宫腔上皮及腺上皮中表达,其中,妊娠第1~3天表达量较高,第4天表达量较低,第5天着床点和非着床点均不表达。孕酮诱导Gsto1的表达,雌激素不能诱导Gsto1的表达,并能抑制孕酮对Gsto1的诱导。Ru486降低孕酮对Gsto1的诱导,孕酮处理1、3、12 h均促进Gsto1的表达,但作用24 h后,抑制Gsto1的表达。结论 Gsto1在小鼠妊娠早期子宫腔上皮及腺上皮中表达,雌激素能够拮抗孕酮对Gsto1的诱导,孕酮可以通过孕酮受体调节Gsto1的表达,并且具有短时调节作用。Objective To investigate the expression and regulation of glutathione S-transferase Omega-1( Gsto1) in the mouse uterus during embryo implantation. Methods A total of 105 CD1 mice were divided into the normal pregnancy model and steriod hormone treatment model. Uterus were collected from days 1 to 5 of pregnancy in normal pregnancy model,and Gsto1 expression was detected by Real-time PCR,in situ hybridization and Western blotting. Ovariectomized mice were used in the steriod hormone model after 2 weeks,and divided into estrogen and progesterone treatment group,progesterone treatment course group,progesterone receptor antagonist Ru486 treatment group. Sesame oil was used for the control of all groups. In the estrogen and progesterone treatment group,uterus was collected after sesame oil,estrogen,progesterone,estrogen plus progesterone treatment 12 hours,respectively. In the progesterone treatment course group,uterus was collected after progesterone treatment 1 hours,3 hours,12 hours and 24 hours,respectively. In Ru486 treatment group,uterus was collected after sesame oil,Ru486,progesterone,Ru486 plus progesterone treatment 12 hours. Gsto1 expression was detected by Real-time PCR,and Western blotting in the steriod hormone model. Results Gsto1 was mainly expressed in the uterine luminal and glandular epithelium on days 1 to 4 of pregnancy. Gsto1 expression was high on day 1 to 3,but became lower onday 4. On day 5,Gsto1 expression was not detected at implantation sites and non-implantation sites. Progesterone induced Gsto1 expression. Estrogen did not induce Gsto1 expression,but inhibited the induction of progesterone on Gsto1. Ru486 reduced the induction of progesterone on Gsto1. Progesterone treatment for 1 hour,3 hours,12 hours promoted Gsto1 expression,but after 24 hours,inhibited Gsto1 expression. Conclusion This study suggests that Gsto1 is mainly expressed in the uterine luminal and glandular epithelium during preimplantation. Estrogen inhibits the induction of progesterone on Gsto1.Progesterone enhances Gsto1 expression by progesterone receptor in short time.国家自然科学基金(31401953);; 安徽省教育厅自然科学重点项目(2014A143

高硫化氢合成气制甲硫醇新型钼基催化剂研究

研究了一系列负载型钼基催化剂催化含高硫化氢合成气制甲硫醇的性能 ,用XPS和ESR对其进行表征 .活性测试显示 ,钾促进的钼基催化剂催化的反应产物中甲硫醇成为主导产物 .几种钼基催化剂合成甲硫醇的活性大小顺序 :K2 MoO4/CoO/SiO2 >K2 MoO4/SiO2 >MoO3 /K2 CO3 /SiO2 >K2 MoS4/SiO2 >MoS2 /K2 CO3 /SiO2 .ESR表征显示 ,反应后的催化剂可以检测到“oxo Mo(V)”和“thio Mo(V)”物种 .XPS表征显示反应后催化剂中的Mo包含着Mo4+ ,Mo5+ 和Mo6+ ,S包含着S2 -,(S—S) 2 -和S6+ 三种价态 .添加CoO后 ,“oxo Mo(V)”含量减少 ,而“thio Mo(V)”含量增加 ,(S—S) 2 -物种的生成得到抑制 ,S2 -物种的量增多 .(Mo4+ +Mo5+ ) /Mo6+ 峰强度比为 0 75以及S2 -/(S—S) 2 -接近 1有利于甲硫醇的生成 .本文提出甲硫醇的合成与“Mo S K”相关联的反应机

Novel Mo-based catalysts for methanethiol synthesis from high H2S-containing syngas

A series of supported Mo-based catalysts for methanethiol synthesis from high H2S-containing syngas were investigated by kinetics and XPS, ESR characterization. The activity evaluating results show that upon the potassium-promoted Mo-based catalysts, the methanethiol will become dominant product of the reaction, and the activity sequence of several Mo-based catalysts for the reaction is as follows: K2MoO4/CoO/SiO2 > K2MoO4/SiO2 > MoO3/K2CO3/SiO2 > K2MoS4/SiO2 > MoS2/K2CO3/SiO2. In the ESR characterization of the catalysts used the resonant signals of "oxo-Mo(V)" and "thio-Mo(V)" can be detected. The XPS characterization indicates that mixed valence Mo species Mo4+, Mo5+ and Mo6+ and three kinds of S species S2-, (S-S)(2-) and S6+ exist in the catalysts. As a promoter CoO was introduced into Mo-based catalysts. It was observed that the addition of CoO leads to decrease of the amount of "oxo-Mo(V) " in the catalysts, but increase of the amount of "thio-Mo(V)", which may be connected with "Mo-S-K" phase or "Co-Mo-S-K" phase, meanwhile CoO in die catalyst was found to enhance the formation of S2-, but inhibit the formation of (S-S)(2-). It was found also that the methanethiol synthesis is favored if the peak intensity ratios of (Mo4+ + Mo5+)/Mo6+ and S2-/(S-S)(2-) are kept at about 0.75 and 1 level respectively. A possible mechanism about the relationship of CH3SH formation with "Mo-S-K" phase was proposed

Effects of Nature Colloids on the Growth of Nature Bacteria Community

利用错流超滤技术提取厦门湾东南部海域、九龙江河口及九龙江水体中的胶体,研究了胶体的来源和胶体有机碳浓度(COC)对天然菌群生长的影响.结果表明,海洋胶体、河口胶体及河流胶体在不同程度上对天然菌群的生长均有促进作用.海洋胶体中的天然菌群相对增长率y为54.0%~96.1%,相对增长率的平均值Y为76.7%;河口胶体中的天然菌群的y为20.0%~97.3%,Y为72.2%;而河流胶体中天然菌群的y为8.3%~112.4%,Y为69.8%.胶体对外加菌群的促进作用与COC浓度没有相关关系.Colloids were abstracted by cross-flow ultrafiltration technique from Xiamen Bay,Jiulongjiang Estuary and Jiulongjiang River respectively.The effects of colloids with different sources and different concentrations of colloidal organic carbon(COC)on the growth of nature bacteria community were studied.The results showed that colloids could apparently improve the growth of nature bacteria community,and the improving effects varied with the different source of colloids,in the colloids from Xiamen Bay,Jiulongjiang estuary and Jiulongjiang River,the raletive increacing rate(y) of nature bacteria community were respectively 54.0%～96.1%,20.0%～97.3% and 8.3%～112.4%,the avarage of raletive increacing rate(Y) were respectively 76.7%,72.2%and 69.8%.The results also showed that there was no correlativity between the avarage of raletive increacing rate(Y) and the concentrations of colloidal organic carbon(COC).国家自然科学基金重大研究计划重大项目(904111016);; 国家教育部高等学校骨干教师计划基金资

The design of a universal electrochemical cell adapted to quartz crystal microbalance measurements

电化学石英晶体微天平(EQCM)同时结合了电化学检测的高灵敏度和石英晶体微天平(QCM)可实时检测电极表面质量变化及阻尼的特点,在电化学研究中具有非常好的应用前景,已得到越来越广泛的应用。本文设计了一种通用型的EQCM电解池,用恒电流电沉积铜的方法测定了QCM PT电极的质量灵敏因子Cf,分析了Cf实验测定值与理论值偏差的原因,并讨论了在所设计的EQCM电解池中QCM PT电极的使用范围,为进一步开展EQCM的应用研究提供可靠的基础。As the electrochemical quartz crystal microbalance(EQCM) is both of the high sensitivity of electrochemical investigation and the ability of detecting the variation of the surface mass and damp in situ as the quartz crystal microbalance(QCM),it gets more popular in electrochemical field.Aiming at the disadvantage of the traditional electrochemical cell,we have developed a universal EQCM cell adapted to electrochemical measurement.The design and characteristics of the new cell were presented.The sensitivity factor of the microbalance was determined by copper electro-deposition.Moreover,the reasons of the difference between the theoretical and experimental values of the sensibility factor was analyzed,then the EQCM using range was discussed.国家自然科学基金资助项目(20433040);福建省教委科研基金资助项目(JA07160

利用双势垒结构研究磁场下二维电子的态密度

利用双势垒结构磁电容曲线，测量了垂直磁场下二维电子态密度。采用高斯型朗道 态密度模型计算了双势垒结构的电容随磁场的变化曲线，与不同偏压和温度下的实验曲线符合得相当好，由此得到朗道能级模型态密度

磁隧穿振荡研究GaAs/AlAs双势垒结构中的Г-X电子态混合

报道了非对称GaAs/AlAs双势垒结构(DBS)中的Γ-X-Γ磁隧穿振荡现象,用磁场倒数周 期求得AlAs层中X谷和GaAs层中Γ谷之间的能带不连续值与通常公认值符合很好.良好的 振荡特性可作为定量研究Γ-X耦合强度的灵敏的实验办法

Socio-environmental impacts and social licence: A critical review and future directions

在经济迅猛发展的今天,民众的环境和生态意识不断提高,参与保护环境行为的积极性也不断增强。因此,项目开发方要想在某地能够顺利地实行一些可能会引发环境问题的项目,除了必要的法律许可外,还应获得并维持当地居民的同意与支持,即社会许可。没有获得社会许可的项目开发面临着多种风险并往往为此付出很大的经济代价。虽然,社会许可在矿业、化学工业等相关领域已经得到了广泛关注,但是近年来这一概念才逐渐受到了心理学家的重视。本文回顾了社会许可的概念起源、测量范式,以及获得和维持社会许可的途径,并在此基础上总结了社会许可的影响因素和相关政策建议。最后,针对社会许可测量和获得中存在的问题,本文从心理学的视角出发,为未来社会许可的研究方向提出建议和展望。</p