Effect of Kangquan Recipe on Apoptosis Regulatory Genes bax and bcl-2mRNA in Prostate of Rats

目的探讨康泉方对实验性大鼠前列腺组织凋亡调控基因bax mRNA、bcl-2 mRNA的影响。方法采用大鼠去势后注射丙酸睾丸酮致前列腺增生法造模,同时灌胃给药30天后,处死大鼠取前列腺组织并测量湿重,RT-PCR法检测各组前列腺腹叶组织bax mRNA、bcl-2 mRNA的表达。结果与模型组比较,康泉各剂量组前列腺湿重显著下降(P0.05);与模型组比较,康泉各剂量组bax mRNA及bax/bcl-2显著升高(P0.05)。结论康泉方对大鼠良性前列腺增生有明显的治疗作用,其作用机理可能通过调节前列腺组织bax mRNA、bcl-2 mRNA表达,促进前列腺细胞凋亡,并呈明显量效关系。Objective To investigate the effect of Kangquan Recipe (KQR) on apoptosis regulatory genes bax and bcl-2 mRNA in prostate of rats. Methods Benign prostatic hyperplasia model rat was established by injecting testosterone after castration. The model rats were killed and prostate glands were removed for examination after being treated with administration of KQR by gastrogavage for 30 days. The wet weight of prostate was measured and the mRNA expressions of bax and bcl-2 in rats' tissue of abdominal lobe of prostate were determined by RT-PCR. Results Compared with the model group, wet weight of prostate was lower significantly in the groups treated with different dosages of KQR (P0.05). Compared with the model group, the expressions of bax mRNA and ratios of bax/bcl-2 were significantly higher and the expressions of bcl-2 mRNA significantly lower in the KQR treated groups (P0.05). Conclusion KQR shows an obvious treatment effect on rats with benign prostatic hyperplasia, the mechanism might be through effectively regulating the expressions of bax mRNA and bcl-2 mRNA in prostatic tissue to accelerate the cell apoptosis of prostate in obvious dose-effect manner.福建省中医药科研项目(No.wzy0616);; 厦门市医学科研项目(No.WSK0526);; 厦门市卫生青年创新课题(No.WQK0510

Effect of Kangquan Recipe on Basic Fibroblast Growth Factor of Prostate Tissue in Rats

目的探讨补肾通络的康泉方对实验性大鼠前列腺组织碱性成纤维细胞生长因子(basic fibro-blast growth factor,bFGF)表达的影响。方法采用大鼠去势后注射丙酸睾丸酮致前列腺增生法造模,同时灌胃中药康泉方30天后,取大鼠前列腺组织,采用免疫组化和逆转录聚合酶链式反应(RT-PCR)法分别检测各组前列腺腹叶组织bFGF表达。结果与模型组比较,康泉高、中剂量组bFGF和bFGFmRNA表达明显降低(P0.05)。结论康泉方能够降低实验性良性前列腺增生大鼠前列腺组织bFGF表达。Objective To investigate the effect of Kangquan Recipe(KQR)on basic fibroblast growth factor(bFGF)in prostatic tissue of rats.Methods Benign prostatic hyperplasia model rat was established by injecting testosterone after castration.After being administered with KQR by gastrogavage for 30 days,the model rats were killed and their abdominal lobe prostate glands were taken for determining the protein and mRNA expressions of bFGF using immunohistochemical method and RT-polymerase chain reaction(RT-PCR)respectively.Results The expressions of bFGF and bFGF mRNA were significantly lower in the model rats being treated with high or medium dose of KQR than those in the untreated model rats(P0.05).Conclusion KQR can effectively decrease the expression of bFGF in prostatic tissue of experimental benign prostate hyperplasia model rats.福建省中医药科研项目(No.wzy0616);; 厦门市医学科研项目(No.WSK0526);; 厦门市卫生青年创新课题(No.WQK0510