锯缘青蟹生殖肢腺体的结构与功能

青蟹雄性腹肢具有生殖肢腺体(GTG).雄性第一腹肢向末端逐渐变成一细薄的弯曲突起.腺体主要集中于腹肢距基部长度的2/5处,即第二腹肢入口处的附近,末端也有少量分布.腺体在组织学上和玫瑰状的皮腺相似,群绕着射精管腔.经染色表明腺体含有中性粘多糖(NMPS)和酸性粘多糖(AMPS),同一批腺体,甚至同一个腺体两种类型糖类的染色状况有异.腺体由分泌细胞和管细胞组成,管细胞含有一导管,多个导管形成网络.导管末端通向交接器内壁表皮开孔处,孔径约为2.5~3.5μm,这些孔通向交接器管腔,孔中及开口处分泌物的染色性质和腺体中相似.第二腹肢也具有腺体,但分布较少,且不太集中.GTG分泌物可能具有几个复杂的功能,其中低粘度的NMPS可能有助于加速强粘性精液通过狭窄的交接器管腔作用,而高粘度的AMPS功能则更为复杂. 【英文摘要】 The gonopod tegumental glands(GTG) of Scylla serrata grouped about the ejaculatory canal.The first gonopod tapered quickly to a thin,curved process terminating in a point.Tegumental glands were located at approximately 2/5 of the gonopod length,also some situated much further between approximately 3/5 or 4/5 of the gonopod length.GTG resembling the PTG(pleopod tegumental glands).The same group glands or a single gland show different intensely staining neutral mucopolysaccharide(NMPS) and an intensely staini...国家863项目(2002AA603013)资

味觉厌恶性条件反射建立后脑内c-Fos的表达

以新异味觉刺激糖精水的摄入为条件刺激，以腹腔注射环磷酰胺（ＣＹ，免疫抑制剂）或氯化锂（ＬｉＣｌ）为非条件刺激，分别使大鼠建立味觉厌恶性条件反射。在条件刺激日，糖精水在学习组大鼠下列脑区中诱发出密集的Ｆｏｓ表达：下丘脑、杏仁核、边缘皮质等，而非学习组在这些区域中却没有或只有少量表达。另外，在丘脑前背侧核、扣带回、下丘脑外侧核、穹隆下器、压部后颗粒皮质、视上核，ＣＹ组的Ｆｏｓ表达明显多于ＬｉＣ１组；而在伏核、杏仁基底外侧核、腹外侧隔核，ＬｉＣｌ组的Ｆｏｓ表达明显多于ＣＹ组，这种差异可能是两种药物的不同药理性质所致。</p

Comparative Study on the Activities of Four ATPases in Different Organs and Tissues of Mud Crab,Scylla serrata

【中文摘要】 本实验采用生化方法研究了锯缘青蟹的鳃、肝胰腺和肌肉等不同器官组织中4种类型ATPase活性.结果表明,不同器官组织中同类型ATPase活性各异,Na+K+ ATPase活性由强至弱为鳃>肝胰腺>肌肉.Ca2+ ATPase活性为肌肉>肝胰腺>鳃.Mg2+ ATPase活性为鳃>肌肉>肝胰腺.Ca2+Mg2+ ATPase活性则为肌肉>鳃>肝胰腺.同一器官组织中不同类型ATPase活性也有较大的差异,鳃中Na+K+ ATPase活性最大,其余的依次为Mg2+ AT Pase,Ca2+Mg2+ ATPase,Ca2+ ATPase;肝胰腺中Na+K+ ATPase活性最强,其余的依次为Ca2+Mg2+ ATPase,Mg2+ ATPase,Ca2+ ATPase;肌肉中Ca2+Mg2+ ATPase活性最大,其余的依次为Ca2+ ATPase,Na+K+ ATPase,Mg2+ ATPase.此外,锯缘青蟹同一器官组织中同类型ATPase在不同个体间也存在一定差异.不同器官组织中4种类型ATPase活性各异主要与其所执行的生理功能密切相关. 【英文摘要】 The activities of four kinds of ATPases in gill ,hepatopancreas and muscle of mud crab Scylla serata were studied by biochemistry methods. The results showed that the activities of the same ATPase was different in various organs and tissues. Among organs and tissues, the activity of the Na~+K~+-ATPase is gill>hepatopancreas>muscle in descending order; the activity of the Ca~(2+)-ATPase is muscle>hepatopancreas>gill; the activity of the Mg~(2+)-ATPase is gill>muscle>hepatopancreas; the acti...国家863重大专项(2002AA603013); 福建省海洋生物优良种质和生物活性物质应用基础研究课题资

The artificial simulation of sperm plug of Scylla serrta

采用模拟人工授精、组织学及电子显微镜观察等方法,研究了青蟹精子塞的形成机制。结果发现,青蟹精子塞为雄性精液和雌性纳精囊开口处的分泌物在短时间内发生剧烈反应的产物,它们在短时间内从液态变为胶状结构,证明了精子塞是雌雄蟹的共同产物。雌雄蟹形成的精子塞的作用是,将精液颗粒、精荚等紧密联系在一起,部分精荚被拉伸破裂;有助于防止后续的雌雄交配,保护或保持精荚的完整及具有营养作用。 【英文摘要】 During artifical transfer of spermatophore,the male seminal products and the female spermathecae secretion will occur a intense reaction in several seconds.As a result,the reaction products became a hard and gelatinous form which change strongly from the original liquid state.This product is the sperm plug.The phenomenon proves that the sperm plug of Scylla serrata is the common product of male and female crab.It 's possible to determined the role of either male or female crabs in the formation of the sperm...国家863项目基金资助(2002AA603013

Study on the artificial fertility spermatophore transplantation of the mud crab Scylla serrata

模仿雄性锯缘青蟹第一腹肢,根据其在交配中的作用机制,采用常规滴管为材料,利用酒精灯灼热拉制一个前端弯曲、侧扁、管径大小接近第一腹肢的人工交接器.以无钙离子的人工海水作为青蟹人工授精的缓冲液,缓冲液中加入适量庆大霉素.首次对青蟹采用类似硬壳交配的方式,将成熟雄蟹贮精囊内的精荚和精液成功移植至雌蟹纳精囊中.结果表明精荚的人工移植不会破坏成熟雌蟹纳精囊的内环境,其内精子具正常受精能力.未交配雌青蟹精荚的人工移植没有正常产卵,但性腺得以促进发育,并能进行正常的生殖脱皮. 【英文摘要】 Imitate male first gonopod of male mud crab, based on its function in reproduction and mate, an artificial mate organ is manufactured. The spermatophores are extended by the Ca~(2+)-FSW, gentamicin is also appended in the extender. With the similar hard carapace mate methods, the spermatophores and semen are transferred to spermathacae of female mud crab artificially. As a result, the mature female mud crab can spawn, though the number of eggs laid is affected. The transferring in unmated female may not spa...国家“863”海洋生物技术主题海水养殖种子工程资助项目(2002AA603013

Direct connection between androgenic glands and horacic ganglion of Scylla serrata

采用组织学、扫描电镜方法对锯缘青蟹(Scyllaserrata)促雄腺和胸腹神经团之间的关系进行观察。结果表明,射精管末端存在促雄腺,促雄腺和胸神经团来源的支配第五步足(即游泳足)的神经紧密相连。该神经在游泳足基部的肌肉内部紧贴射精管并行,其分支支配着射精管周围肌肉,可能也是控制精荚排放的神经。本研究可为促雄腺对性别控制的机制及蟹类精荚电促排的深入研究提供理论依据。 【英文摘要】 Androgenic gland hormone (AGH) is responsible for male sexual differentiation in crustaceans. An androgenic gland hormone may allow hormonal sex reversal for the production of monosex populations in aquaculture, leading to increases in production. There are many researches about the androgenic gland and its hormone by different research methods. Because AGH of male crab produces components of seminal fluid that alter the behavior, physiology and life span of mated female, and contribute to her efficient sto...国家“863”高技术研究发展项目(2002AA603013);; 福建省重中之重项目“福建省海洋生物优良种质和生物活性物质的应用基础研究项目”

The artificial simulation of sperm plug of Scylla serrta

【中文摘要】 采用模拟人工授精、组织学及电子显微镜观察等方法,研究了青蟹精子塞的形成机制。结果发现,青蟹精子塞为雄性精液和雌性纳精囊开口处的分泌物在短时间内发生剧烈反应的产物,它们在短时间内从液态变为胶状结构,证明了精子塞是雌雄蟹的共同产物。雌雄蟹形成的精子塞的作用是,将精液颗粒、精荚等紧密联系在一起,部分精荚被拉伸破裂;有助于防止后续的雌雄交配,保护或保持精荚的完整及具有营养作用。 【英文摘要】 During artifical transfer of spermatophore,the male seminal products and the female spermathecae secretion will occur a intense reaction in several seconds.As a result,the reaction products became a hard and gelatinous form which change strongly from the original liquid state.This product is the sperm plug.The phenomenon proves that the sperm plug of Scylla serrata is the common product of male and female crab.It s possible to determined the role of either male or female crabs in the formation of the sperm...国家863项目基金资助(2002AA603013

Direct connection between androgenic glands and horacic ganglion of Scylla serrata

【中文摘要】 采用组织学、扫描电镜方法对锯缘青蟹(Scyllaserrata)促雄腺和胸腹神经团之间的关系进行观察。结果表明,射精管末端存在促雄腺,促雄腺和胸神经团来源的支配第五步足(即游泳足)的神经紧密相连。该神经在游泳足基部的肌肉内部紧贴射精管并行,其分支支配着射精管周围肌肉,可能也是控制精荚排放的神经。本研究可为促雄腺对性别控制的机制及蟹类精荚电促排的深入研究提供理论依据。 【英文摘要】 Androgenic gland hormone (AGH) is responsible for male sexual differentiation in crustaceans. An androgenic gland hormone may allow hormonal sex reversal for the production of monosex populations in aquaculture, leading to increases in production. There are many researches about the androgenic gland and its hormone by different research methods. Because AGH of male crab produces components of seminal fluid that alter the behavior, physiology and life span of mated female, and contribute to her efficient sto...国家“863”高技术研究发展项目(2002AA603013);; 福建省重中之重项目“福建省海洋生物优良种质和生物活性物质的应用基础研究项目”

Comparative Study on the Activities of Four ATPases in Different Organs and Tissues of Mud Crab,Scylla serrata

本实验采用生化方法研究了锯缘青蟹的鳃、肝胰腺和肌肉等不同器官组织中4种类型ATPase活性.结果表明,不同器官组织中同类型ATPase活性各异,Na+K+ ATPase活性由强至弱为鳃>肝胰腺>肌肉.Ca2+ ATPase活性为肌肉>肝胰腺>鳃.Mg2+ ATPase活性为鳃>肌肉>肝胰腺.Ca2+Mg2+ ATPase活性则为肌肉>鳃>肝胰腺.同一器官组织中不同类型ATPase活性也有较大的差异,鳃中Na+K+ ATPase活性最大,其余的依次为Mg2+ AT Pase,Ca2+Mg2+ ATPase,Ca2+ ATPase;肝胰腺中Na+K+ ATPase活性最强,其余的依次为Ca2+Mg2+ ATPase,Mg2+ ATPase,Ca2+ ATPase;肌肉中Ca2+Mg2+ ATPase活性最大,其余的依次为Ca2+ ATPase,Na+K+ ATPase,Mg2+ ATPase.此外,锯缘青蟹同一器官组织中同类型ATPase在不同个体间也存在一定差异.不同器官组织中4种类型ATPase活性各异主要与其所执行的生理功能密切相关. 【英文摘要】 The activities of four kinds of ATPases in gill ,hepatopancreas and muscle of mud crab Scylla serata were studied by biochemistry methods. The results showed that the activities of the same ATPase was different in various organs and tissues. Among organs and tissues, the activity of the Na~+K~+-ATPase is gill>hepatopancreas>muscle in descending order; the activity of the Ca~(2+)-ATPase is muscle>hepatopancreas>gill; the activity of the Mg~(2+)-ATPase is gill>muscle>hepatopancreas; the activity of the Ca~(2+...国家863重大专项(2002AA603013);; 福建省'海洋生物优良种质和生物活性物质应用基础研究'课题资