20 research outputs found

    The pathogeny of ulcer disease in Epinephlus awoara

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    2002年夏季厦门同安湾一带气候炎热,降雨量少,海水盐度偏高,同安湾刘五店的网箱养殖石斑鱼大面积暴发溃疡病。本研究调查了石斑鱼溃疡病暴发的特征和症状,主要表现为肌肉溃疡坏死、眼球脱落、鱼骨暴露等。从病鱼体表及内脏分离出优势菌群命名为TS 628,经回归感染证实TS 628就是引发本次石斑鱼溃疡病的病原菌。对病原菌进行鉴定发现,该菌革兰氏染色呈阴性,电镜下观察菌体呈短杆状,极端单鞭毛,综合研究该菌在形态、生理生化、16SrDNA同源性及药物敏感性等方面的特性,基本确认分离到的病原菌为哈维氏弧菌(Vibrioharveyi),该菌对氯霉素、壮观霉素等多种抗生素敏感,对万古霉素、青霉素G等抗生素不敏感。哈维氏弧菌是水产养殖病害常见病原菌,但作为养殖石斑鱼的病原菌在国内属首次报道。The summer of 2002 was hot and dry in Xiamen, and the seawater salinity was higher than usual, sometimes reaching 38 in Tongan Bay. From the end of May, ulcer disease occured on a large scale in Tongan Bay. In the middle of June, a heavy rain brought about high mortality among the cultured groupers, which caused serious loss. The characteristics of ulcer disease were observed. Infected groupers displayed sluggish swimming and refused feeding, several days later, the groupers' eyes swelled and became ulcerated, the tail turned red, the scales became detached and the back muscle gradually ulcerated, then the eyes even dropped out and the muscle became so necrotic that the spine was exposed, finally the diseased fishes died. Anatomized the dead fish and found that the livers and gills were pale and the gallbladder was distended. The time course from appearance of disease signs to death lasted about a week. Dominant bacteria, which were Gram-negative and seen short rod with single polar flagellum under electron microscope, were isolated and designated TS-628. In artifical infection test all fish of the experimental group died on the third day after injected with bacterial suspension, while all the fish in control group showed no signs till the end of another week observation. The dead grouper had pale livers and gills and small ulcerations on the caudal fin and anal fins. These were the same signs as the natural infected fishes. The re-isolate also had the same characteristics as TS-628, which proved the isolate TS-628 was the pathogenic bacteria that triggered this ulcer disease. Different methods were used to identify the pathogenic bacteria in this study. The identification result through VITEK-AMS system GNI card was that TS-628 was Burkholderia mallei, with 99% reliability. While traditional biochemical identification revealed that TS-628 exhibited relVibrio harveyi through comparison with Bergey's Manual Determinative Bacteriology. In order to confirm absolutely different results above, further researches were carried out to identify TS-628 again. So 471bp sequence of TS-62816S rRNA gene was amplify and compared with all DNA sequences (1192858 in total) in genebank (GenBank+EMBL+DDBJ+PDB), homology analyses showed that 16 sequences were picked out to have the highest similarity, with 98% identity. These 16 sequences all originated fromV. harveyV. carchiariae which also belongs to harveyi because their similarity in physiological and biological characteristics and DNA homology. According to morphological features, physiological and biochemical characteristics and 16S rRNA gene homology comparison of the bacteria, the patV. harveyi. Drug sensitivity test showed that the pathogenic bacteria were highly sensitive to chloramphenicol, actinospectcinV. harveyi is a kind of pathogenic bacterium commonly found iV. harveyi is reported as the pathogenic bacteria of cage-cultured grouper in China. And V. harveyi should be regarded as an opportunistic pathogen which has close relation to temperature and salinity and easily causes vibriosis under conditions of high temperatures and drought. Therefore, it is necessary to guard agV. harveyi vibriosis in such summer days.福建省重大科技项目资助(2002N009

    现代杂技的受众认同与剧场营销——以广西杂技团童话杂技剧《猫猫侠:保卫蝶苑》为例

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    在杂技主题化、剧目化的时代发展趋势下,实现杂技节目、杂技剧的剧场营销与市场经济转型成为一种必然的要求,可以说现代杂技的剧场营销与市场经济转型需要精确的受众定位与节目"供给"。广西杂技团编创的集童话的梦幻纯真,与杂技的趣味、游戏于一体的童话杂技剧《猫猫侠:保卫蝶苑》,以歌、舞、技及童话跨界综合的创新与探索,展现了现代杂技艺术在受众定位与剧场营销方面的方向与可能。“广西本土语言文学研究中心”建设经费资助项目 2017国家社科基金艺术学项目“中国当代杂技创作研究”(项目编号:17BE093)的阶段性成

    葱郁在“南高原”的故乡——谭宁君诗歌论

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    就当代诗歌的“故乡”书写而言,“故乡城市化”关注是其重要趋势,谭宁君“冷暖”两种乡愁书写呈现了这种复杂的故乡书写。在关于故乡诗性的意象、句式结构及主题里,他完成了对故乡的生命诗写和诗性言说,完成了对故乡与故乡乡愁的诗性守望及故乡书写意义的探寻。2011国家哲学社会科学基金项目“朦胧诗以来现代汉语诗歌的语言问题研究”(11BZW096

    通感写作与百年新诗

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    "通感"也即"象征",中国新诗"格律诗派""象征诗派""现代派"等不同流派在横向移植西方象征主义诗学表现手法的同时,以感官互通、彼此感应的"通感"书写建构了中国新诗以"知性""哲理性"为特征的诗性写作。在百年新诗中,具有现代汉语诗歌文体性特征的通感修辞和诗体通感,不仅深化了现代诗人对心灵世界与周围世界的诗性感应与精神探寻,还呈现了百年新诗本体性的审美方式与写作可能。2011年国家哲学社会科学基金项目“朦胧诗以来现代汉语诗歌的语言问题研究”(11BZW096); “广西民族文化保护与传承研究中心”建设经费资

    现代杂技创作的“剧”性转型——以广西大型壮族杂技剧《百鸟衣》为例

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    杂技艺术是"身体"的艺术,当代杂技不管如何强调技艺自身的"高、险、惊、难、奇、谐"等特点,都不免于形体、道具及舞台所形成的造型表意与造境传情的"局限",导致其与现代剧场艺术讲究与追求意境、情感、主题等审美期待存在距离。面对杂技本身困局、时代需求及未来发展,20世纪90年代中期以来,在市场经济的刺激和压力下,中国杂技开始了从杂技造型、造境到杂技主题化及剧目化的探

    "Illness" Experience and "Dispersion" Depiction --Post-1980-born XIONG Yan's Poetry

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    "病痛"体验是时间与生命留存于身体中的感知"心像","病痛"书写直指时间与生命一体的诗性内核。80后诗人熊焱关于时间与生命的"病痛"体验,表现着个人、时间、生命三个维度的孤独本质,他由"病痛"时刻与状态完成的诗歌表现出强烈的孤独诗感。时间与生命的"离散"书写,完成了熊焱个体对时间与生命的解释与意义探寻。"Illness" experience is the bodily "heart - felt image" retained by time and life, so illness depiction points to the poetic core of time and life. Post-1980-born XIONG Yan' s illness experience in regard to time and life displays solitude nature in three dimensions, concerning individuality, time and life. His poems about illness moment and completion display a strong poetic solitude. XIONG Yan's dispersion depiction about time and life proclaims his interpretation and pursuit to their significance.2011国家哲学社会科学基金项目“朦胧诗以来现代汉语诗歌的语言问题研究”(项目编号:11BZW096)的阶段性成

    现代杂技的观众认同与剧场营销——以广西杂技团童话杂技剧《猫猫侠》为例

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    在杂技主题化、剧目化的发展趋势下,实现杂技节目、剧目的剧场营销成为一种必然的要求。广西杂技团编创的童话杂技剧《猫猫侠:保卫蝶苑》,以歌、舞、技及童话的跨界创新表演形式,吸引了一大批"儿童家庭"观众,从而以一种精确而稳定的观众定位与剧目"供给",实现了杂技的剧场营销与市场经济转型的内在意蕴。一、梦幻情

    现代杂技创作的“剧”性转型(续)——以广西大型壮族杂技剧《百鸟衣》为例

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    作为一种剧场艺术,现代杂技剧的"审美创意"和"形上营销"的丰收始于并完成于剧场。剧场不仅是一个具体而有限的物理空间,更是一个演员演戏、观众看戏的审美空间。在剧场里,杂技剧能够创造出演员与观众共同想象的审美与体验世界。因杂技技艺的难度提升以及审美特征的有限性,杂技在保持已有技巧难度的前提下,可以增加外延来求得发展。现代的杂技剧以"剧"的情感与审美,统制杂技的"高、险、惊、难、奇、谐"所形成的技艺高度,把杂技造型的表意与传

    新马戏创作与后戏剧勘探——以中国首部新杂艺实验剧《Touch——奇遇之旅》为例

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    新马戏是20世纪80年代以来西方马戏和戏剧艺术发展的重要潮流,其戏剧性主题、舞台综合运用与跨国联合创编的特征既为马戏艺术创造了新活力,又为当代戏剧艺术带来了新变革。受后戏剧剧场发展的影响,新马戏对日常生活、异质空间、创意空间与心灵世界"物我合一"的剧场性演绎,让其更加适应现代观众的审美。中国杂技团以新马戏的艺术理念,通过跨国联合创编,于2018年5月成功上演了首部新杂艺实验剧《Touch——奇遇之旅》。这部作品不仅展示了中国当代杂技艺术在新马戏与剧场艺术领域的积极探索,而且还推动了中国当代杂技艺术继杂技主题晚会、杂技剧之后的又一次转型,具有重要的开拓意义。本文为广西民族文化传承与保护研究中心建设经费资助项目和2017年度国家社科基金艺术学 项目“中国当代杂技创作研究”(项目编号:17BE093)的阶段性

    Promoting New Urbanization Construction in Minority Areas with the Rule of Law Thinking

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    现代城市文明与几千年的农耕文明是一对双生子。城镇化是民族地区经济社会发展的必然过程,就地就近城镇化是民族地区新型城镇化的路径抉择。必须依法强化民族地区城镇建设规划的权威性,凸显民族地区城镇建筑设计的"本土化",推进民族地区城镇治理的民主化和法治化,健全民族地区绩效考评和责任追究机制。Evolved over a long history of thousands of years, agricultural civilization and modem urban civilization are twins. In Minority Areas, urbanization is a inevitable course of the socioeconomic development, accordingly, there are several main approaches to take the choice of pathway with the principle of situ and proximity: to strengthen the authority of the urban construction planning in accordance with the law, to highlight the "localization" in urban architectural design, to promote urban governance through democratization an leglslation, to perfect the mechanism about performance appraisal and accountability.2014年度国家社会科学基金重大项目“建设社会主义民族法学体系、维护民族大团结研究”(批准号为:14ZDC025)的研究成果之
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