Airborne fine particle decreases the cell viability and induces inflammation in human bronchial epithelial cells

目的:研究大气细颗粒物(fine particulate; matter,PM_(2.5))对人支气管上皮细胞活性的影响及其炎性作用。方法:用PM_(2.5)采样器采集上海地区大气PM_(2.5)样本,扫; 描电镜观察PM_(2.5)形态特征。将人支气管上皮细胞BEAS-2B暴露于不同浓度(0,50,100,200,400,800; mug/mL)的PM_(2.5) 12,24,48 h,细胞活力检测试剂盒(cell counting kit-8,; CCK-8)法检测PM_(2.5)暴露对细胞活性的影响。实时定量PCR(quantitative real-time; PCR,qRT-PCR)检测细胞粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating; factor,GM-CSF)和TNF-alpha mRNA的表达,Western印迹检测; GM-CSF和TNF-alpha蛋白的表达。结果:扫描电镜观察发现,PM_(2.5)形态多样,大小不一,直径大多等于或小于2.5; mum。与同时间点未暴露组比较,各暴露组(50~800; mug/mL)细胞活性呈不同程度的下降,差异具有统计学意义(P<0.05)。与未暴露组比较,暴露于100,400或800 mug/mL; PM_(2.5) 24 h后,GM-CSF和TNF-alpha; mRNA和蛋白表达水平明显升高(P<0.05),且PM_(2.5)暴露浓度越高,GM-CSF和TNF-alpha的mRNA和蛋白升高水平越显著。; 结论:大气PM_(2.5)可引起人支气管上皮细胞的炎症反应,降低细胞活性,这可能与PM_(2.5)促发和加重支气管肺部炎性疾病有关。Objective: To investigate the effects of airborne fine particle on cell; viability and inflammation in human bronchial epithelial cells. Methods:; Atmospheric PM_(2.5) samples were collected by PM_(2.5) sampler.; PM_(2.5) morphology was observed by scanning electron microscope (SEM).; Human bronchial epithelial cells (BEAS- 2B) were treated with PM_(2.5); at different concentrations (0, 50, 100, 200, 400, 800 mug/mL) for 12,; 24 or 48 hours, and the cell activity were evaluated by cell counting; kit-8 (CCK-8). The mRNA expression levels of (granulocyte-macrophage; colony stimulating factor,GM-CSF) and TNF-alpha were detected by; quantitative real-time PCR (qRT-PCR). Western blot was used to detect; the protein expressions of GM-CSF and TNF-alpha. Results: According to; SEM, the shape of PM_(2.5) varied, and the diameter was different and; mostly equal to or less than 2.5 mum. CCK-8 assay showed that different; concentrations of PM_(2.5) exposure for 12 hours, 24 hours and 48 hours; resulted in loss of cell viability of BEAS-2B cells (P<0.05). Different; concentrations of PM_(2.5) increased the mRNA and protein expression of; GM-CSF and TNF-alpha, and the higher concentration of PM_(2.5) induced; higher expression, which have statistical significant difference between; the groups (P<0.05). Conclusion: Atmospheric PM_(2.5) can cause; inflammatory response in human bronchial epithelial cells. They can; reduce cell viability, which may be related to the PM_(2.5) trigger and; aggravation of bronchopulmonary inflammatory diseases

Research on T Lymphocyte Subsets and NK Cells Activity in Peripheral Blood of Hypopharyngeal Squamous Cell Carcinoma

目的:分析下咽鳞状细胞癌患者外周血T淋巴细胞亚群和NK细胞活性情况,旨在为相关研究工作提供参考资料。方法:择取2011年6月-2016年5月本院收治的95例下咽鳞状细胞癌病患为病例组,另取此期间内来本院接受健康检查的72例志愿者为正常组。使用流式细胞仪对病患开展相关检查,分析病例组与正常组的淋巴细胞亚群、NK活性情况以及不同临床分期的下咽癌病患外周血T淋巴细胞亚群以及NK细胞活性之间的关系。结果:与正常组相比,病例组的NK细胞、总B淋巴细胞、总T淋巴细胞、辅助/抑制T细胞、辅助T细胞偏低(P0.05)。Ⅰ~Ⅱ期病患辅助T淋巴细胞比Ⅲ~Ⅳ期者略高(P>0.05)。Ⅰ~Ⅱ期病患抑制T淋巴细胞值比Ⅲ~Ⅳ期者低(P0.05)。Ⅰ~Ⅱ期病患外周血NK细胞数比Ⅲ~Ⅳ期者略高(P>0.05)。结论:下咽鳞状细胞癌病患的免疫功能异常,且随着疾病的进展,细胞免疫和体液免疫均有所降低,证实免疫功能紊乱在疾病进展中发挥了一定作用。经流式细胞设备对外周静脉血淋巴细胞亚群以及NK细胞活性情况检测,可实现动态化检测癌症病患某阶段细胞免疫功能变化详情,此法方便简单,在指导治疗方案制定以及监测疾病预后方面,均体现出了一定效果,值得进一步推广

Sarcomatoid Carcinoma of Hypopharynx： A Report of 5 Cases

目的探讨下咽肉瘤样癌的临床及病理学特点,为下咽肉瘤样癌的诊治提供参考依据,提高对肉瘤样癌的诊断、治疗及其预后的认识。方法分析2010年1月—2014年9月我院治疗的5例下咽肉瘤样癌患者的临床资料,包括诊疗过程、病理特征等。结果 5例患者均经过不同的手术方式,其中咽后壁扩大切除1例,梨状窝扩大切除2例,全后、全下咽、全食管切除2例;肉瘤样癌肉瘤样成分与癌成分共存,且之间有一定的移行,CK、vimentin均表达阳性,P63阳性4例。5例患者均定期随访。结论下咽肉瘤样癌有其特殊的生物学行为及病理学特点,是一种不同于其他类型的恶性肿瘤,具有自身病理特征,预后情况各不相同,外科手术仍是首选治疗方法,术后辅助放化疗。Objective To explore the biological behavior and clinical features of the sarcomatoid carcinoma（SC） of hypopharynx for improving the diagnosis,treatment and judgment of prognosis of the tumor.Methods Clinical data,including treatment process and pathology features of the 5 cases of SC underwent operation in our department from January 2010 to September 2014 was analyzed retrospectively.Results All the five patients underwent different surgical procedures and all survived,one case received pharyngeal extended resection,tow cases received pyriform extended resection and tow cases received total throat、swallow、esophagectomy resection.The biological of sarcomatoid carcinoma of head and neck performed components and cancer components coexisted,and has a certain shift between them,CK and vimentin expressed was positive in all cases,P63 was positive in 4 cases.All patients were followed up regularly.Conclusion Sarcomatoid carcinoma of hypopharynx has its special biological behavior and pathology characteristics,it is different from other types of malignant tumors,and has its own pathological features,surgeryis is still the preferred method,postoperative assistance chemotherapy is needed

東アジアにおける法を活用した規範教育の構築－市民性と人権感覚に支えられた規範意識の醸成－

research repor

Expression and significance of p-catenin inhibitor Chibby in Nasopharyngeal Carcinoma

第一作者简介及通讯: 蔡成福, 男, 福建人, 医学硕士, 副主任医师, 主要研究方向为头颈肿瘤的临床工作 E-ma il:d yy- [email protected] 通讯作者. 赵德安(E- mail:[email protected])[中文文摘]目的研究β-catenin拮抗因子Chibby在鼻咽癌组织中的表达情况,并探讨其与鼻咽癌临床病理因素的相关性。方法分别采用Real time PCR及免疫组织化学(SABC法)检测45例鼻咽癌及癌旁正常鼻咽黏膜组织中Chibby mRNA及蛋白的表达情况,统计Chibby的表达与鼻咽癌患者临床病理因素的关系。结果 Chibby mRNA在癌旁正常鼻咽黏膜及鼻咽癌组织中平均相对表达量分别为0.0835±0.0056和0.0776±0.0059,Chibby蛋白在癌旁正常鼻咽黏膜及鼻咽癌组织中的阳性表达率分别为91.1%(41/45)和42.2%(19/45)。鼻咽癌组织中Chibby的表达与T分期及临床分期有相关性,与性别、年龄、颈淋巴结转移及病理分型无明显相关性。结论与癌旁正常鼻咽黏膜组织相比,鼻咽癌组织中Chibby mRNA及蛋白的表达均降低,Chibby的表达与肿瘤T分期及临床分期相关,与性别、年龄、颈淋巴结转移及病理分型无明显相关性,Chibby可能参与了鼻咽癌的发生过程,有望成为鼻咽癌基因治疗新的作用靶点。[英文文摘]OBJ ECTIVE TO investigate the expression of Chibby a new β-catenin inhibitor in nasopharyngeal careinoma , and to explore the relationship between Chibby and clinicopathological features of nasopharyngeal carcinoma . METHODS Real time PCR and SABC im munohistochemical staining were used to detect the expression of Chibby mR N A and protein in nasopharyngeal carcinoma tissues and nasopharyngeal normal tissues of 45 cases , respectively .The re!ationships between Chibby expression and cIinicopathological features the gender, age , T stages .Iymph node metastasis , clinical stages and pathological grade were analyzed by statistics . RULTS The expressions of Chibby mRNA were 0 .0835士0 .0056 and0.0776 士0.0059 in nasopharyngeal normal tissues and nasopharyngeal carcinoma tissues, respectively.The positive expressive rates of chibby protein were 91.1 % (41/45 ) and 42 .2 % (19/45 ) in nasopharyngeal norma l tissues and nasopharyngeal carcinoma tissues,respectively.There were eminent relation between the lower expression of Chibby in nasopharyngeal careinoma and T stages and clinical stges , but no relation with the sex , age , metastasis of lymph and pathological grades .CONCLUSION Compare with normal nasopharyngea l tissues, the expressions of Chibby mRNA and prote in were reduced in nasopharyngeal carcinoma tissues.The Iower expressive levels of Chibby were associated with the T stages and clinica l stages of carcinoma , but no relation with the sex , age , metastasis of Iymph and pathological grades.Chibby may be involved in the pathogenesis of nasopharyngeal carcinoma ,and be expected to become a new target of gene t herapy of it

独龙江河流生态系统健康评价

于2018年10-11月,对独龙江水系干流及支流的河流物理生境、水体理化性质和大型底栖动物进行调查,并根据独龙江自身特点,运用改进的河流健康综合评价指数对该地区生态系统健康状况进行了评价。结果表明:该地区调查样点中85.2%的样点处于健康状况,仅人口密集的乡镇附近健康状况处于亚健康水平,其中旅游业发达的独龙江乡健康状况最差。河流是水资源的重要载体,随着当地旅游业的不断发展,科学评价当地河流的健康状况可以为独龙江河流生态系统修复、水资源的合理开发利用及可持续发展提供重要的科学依据

One stage surgical treatment of multiple primary carcinoma of hypopharynx and esophagus

目的探讨下咽、颈胸段食管多原发癌(multiple primary carcinoma,; MPC)在胸腔镜辅助下行全喉、下咽、食管切除并管状胃重建一期手术的应用及疗效。方法胸科组行胸腔镜辅助下分离食管及纵膈淋巴结清扫后开腹行管状胃成形; ,头颈组行颈部淋巴结清扫、全喉下咽切除、咽胃吻合术。术后常规补充放化疗。结果本组全部病例均一期完成手术,肺部感染3例,胸腔积液2例、气管撕裂1例; ;无吻合口瘘及围手术期死亡病例;3年生存率63.6%,5年生存率50.0%。结论下咽癌应常规行胃镜检查以免MPC的漏诊;胸腔镜辅助下全喉、下咽、; 食管切除并管状胃重建术可一期完成以往分次手术难以完成的手术治疗,有效提高下咽颈胸段食管多重癌的治疗效果。OBJECTIVE To discuss the therapeutic effect of one stage surgical; treatment in the multiple primary hypopharyngeal and cervical thoracic; esophageal carcinoma. METHODS The thoracoscopy group: dissecting the; esophagus and mediastinal lymph node assisted with thoracoscope, and; then opened abdominal cavity to make gastric tube. Head and neck group:; doing the cervical lymph node dissection, total laryngectomy, total; hypopharyngectomy and total esophagectomy, and then anastomosis of the; pharynx with gastric tube. All cases were received conventional; radiotherapy and chemotherapy after operation. RESULTS All the cases in; this group were successfully underwent the one stage operation. The; postoperative complications were pulmonary infection in 3 cases, pleural; effusion in 2 cases and tracheal tear in one case. No anastomotic; fistula or postoperative deaths occurred. The 3 and 5 year survival; rates were 63.6% and 50.0% respectively. CONCLUSION It should take; necessary examinations of cervical thoracic esophagus to prevent missing; the multiple primary carcinoma of the hypopharyngeal carcinoma. The; total laryngectomy, total hypopharyngectomy and total esophagectomy, and; anastomosis of the pharynx with gastric tube for multiple primary; hypopharyngeal and cervical thoracic esophageal carcinoma is a feasible; and active treatment method