梯度密度离心法分离肝癌细胞及其转移性细胞株的建立

【目的】建立新的肝细胞癌细胞系，为肝癌实验研究提供更多纯化癌细胞。【方法】从肝细胞癌患者原发灶和门脉癌栓中获取癌组织，采用Percoll浓度梯度分离癌细胞进行培养，用微环境消化法获取癌细胞克隆并建立细胞系，对培养成功的细胞系进行生长曲线测定、染色体G带核型分析、对比基因组杂交和裸鼠接种成瘤实验。【结果】对20例肝癌组织进行癌细胞分离培养和克隆，成功建立2例细胞系H2M和H4M，这2株细胞均来自门脉癌栓，它们的染色体数目测定均为超3倍体（71．78条）。CGH检测显示H2M主要的细胞遗传学改变为染色体4q、13q、16q、17p和19p缺失和1q、3q、5p、6p、7q和8q扩增，其中有一条标记染色体含有1q和6p[t（1；6）染色体]；而H4M染色体8p，9，13q，16q缺失和6p，7p,11p，11q13扩增，其中有一条标记染色体含有一长的均染区（hsr）。H2M细胞接种裸鼠1个月，产生典型的人肝细胞癌，但H4M接种尚未能成瘤。【结论】Percoll浓度梯度分离癌细胞和微环境消化法取得癌细胞克隆是建立原代细胞系的有效方法。2例肝癌细胞系的建立为以后肝癌研究提供了实验材料，所测出的肝癌细胞系的细胞遗传学改变及在裸鼠接种成瘤．为肝癌细胞癌基因和抑癌基因筛选提供了研究线索和实验模型

Development tactics of apparel CAD/CAM in China

介绍了服装CAD／CAM在中国的发展现状,分析了存在的主要问题,提出了软硬件开发企业合并重组、国家有关部门及行业协会加强管理、加强与国外企业的合作等发展策略,供有关生产企业参考．The current situation of apparel CAD/CAM development in China is introduced, and the main problems therein are analyzed. The development tactics, which include the consolidation and regroupment of corporations developing software and hardware, the national departments and guilds strengthening management, and reinforcing the cooperation with the overseas corporations, are advanced for the references of manufacturing enterprises in this line

深圳福田白骨壤红树林生物量和能量研究

本文探讨了深圳福田红树林保护区５６年生白骨壤群落的生物量、生长量及能量分布。结果表明：该群落现存生物量为１６５８３８８ｇ／ｍ２，其中地上部占６６７８％，地下部占３３２２％。年增长量（１９９３）为１１４９８ｇ／ｍ２。植物体各组分热值含量变化范围在１６１～１８２２ＫＪ／ｇ，其中以树干材热值含量居最高，而中根最低；群落能量现存量为２９１８３９ＫＪ／ｍ２，其中地上部占６８５１％，地下部占３１４９％；群落年增长能量存留量（１９９３）为２０２１３ＫＪ／ｍ２

Synthesis and Applications of Carbon Dots

近年来,由于碳纳米材料具有高的催化活性以及好的稳定性等优点,其在科学、工程以及商业领域都得到了广泛的应用。其中新型“零维“碳纳米材料——碳量子点(CArbOn dOTS,CdS)具有荧光信号稳定、无光闪烁、激发波长和发射波长可调控等独特的光学性质,以及生物毒性小和生物相容性好等优势,逐渐成为碳纳米材料的研究热点,广泛应用于生物成像、生物细胞标记、传感器、光催化、太阳能电池以及发光元件等领域。本文主要综述了CdS的不同合成方法(包括自上而下法和自下而上法)及其应用。In recent years,nanomaterials have made an important impact on diverse science,engineering,and commercial sectors due to their high catalysis,lowcost,and good stability.Acting as a class of ‘zero-dimensional'carbon nanomaterials,carbon dots( CDs) possess unique optical properties of high photostability against photobleaching,tunable excitation and emission wavelength,as well as lowcytotoxicity and good biocompatibility.Therefore,CDs have become a hot subject of carbon nanomaterial in the past decade,not only for its unique properties but also for its applications in various fields such as bioimaging,biolabeling,sensors,photocatalysis,solar cells, light-emitting element and so on.This article reviews the different synthetic methodologies( including two classes: top-down and bottom-up) to achieve good performance of CDs.At the same time,the applications of CDs are also reviewed in the article.国家质检总局科技计划项目(No.2012QK053); 福建省质量技术监督局科技项目(No.3002A91429); 福建省高校产学合作科技重大项目(No.2012H6026); 福建省自然科学基金项目(No.2012D136); 福建省教育厅科技项目(No.JB14180)资助~

Study on the Model both Cutting Variable-speed of the Log-core Veneer Lathe and Moving Locus Model with Constant-speed Peeling

详细分析了无卡轴旋切机的工作原理,推导了旋切过程中刀刃进给速度与圆木直径的数学模型,同时得出旋切时间的近似计算式。从中可知,旋切过程中刀刃进给速度必须按一定规律变化才能保证旋切机正常工作。建立了旋切机恒线速旋切的运动轨迹模型,推导出旋切机恒线速旋切时间的精确计算式,并在此基础上提出了旋切时间简便的近似计算式。这些研究结果均可用于指导设计和进行实际生产。Working principle of log-core veneer lathe had been analyzed in detail,and the mathematical model between the knife-carriage feeding speed and the diameter of the log core was established,in the meantime,the approximation cutting time formula was also derived.The feeding speed must be changed with continuousness and a rule,which can let the lathe work well.Moving locus model of invariable-speed peeling was also founded,precision calculating formula of invariable-speed peeling was deduced.In the same time,the simplification cutting-speed formula was also derived.These results of the research will provide a guide for the designing and the producing practice

The application and research of the aircraft flexible fixture

主要研究气动、液压与电传技术在航空制造业中装配工艺中的应用。对研制出数控机床气动柔性夹紧系统,及其在飞机上零件加工上的应用进行了详细论述,包括柔性系统的机构组成、工作原理及PlC控制系统。该技术的突破为我国飞机数字化加工装配提供了参考价值,并将促进航空制造业的跨越式发展。The application of the pneumatic,hydraulic and power transmission for assembly process and equipment in aviation manufacturing industry is study.A novel type of CNC Aircraft Flexible Fixture machine system has been made out,and applied in manufacturing the aircraft parts.The system is including in architecture of the composition,working theory,PLC control system.It has a great signification of the manufacturing technology for aircraft digital processing,manufacturing and assembling,and promoting the developing of aircraft manufacturing

Adaptive management of aquatic germplasm reserves

针对我国水产种质资源保护区在现行管理模式下普遍存在的资源难以恢复、保护效果不明显等问题,基于生态系统管理的理念,借鉴国内外有关自然保护区适应性管理研究实践经验,探索水产种质资源保护区的适应性管理。水产种质资源保护区的适应性管理可分为模式建立和反馈两个阶段,包括问题识别、目标制定、方案设计与实施、动态监测、绩效评估、反馈与调整等过程,以全面提升保护区对外界环境变化的不确定性和复杂性的快速反应能力。并以福建长乐西施舌资源增殖保护区为例,分析该保护区的管理现状,探索适应性管理模式的构建与应用。There were some common issues of the current management model in domestic Aquatic Germplasm Reserves( AGRs),such as the difficulty of resources recovery and the insignificant efficiency of protection.An Adaptive Management( AM) framework of AGRs was constructed according to the theoretical researches and practices of Ecosystem-based Management( EBM) and Adaptive Management both in domestic and foreign nature reserves.The AM framework of AGRs could be divided into two stages as model establishment and feedback: the former included problem identification,strategic planning,program design and implementation,while the later comprised of dynamic monitoring,performance evaluation,feedback and adjustment.The practice of AM framework could strongly enhance the rapid response to the uncertainty and complexity of external environment of AGRs.Furthermore,the Changle Xishi Tongue( Coelomactra Antiquata) Resource Enhancement and Protected Area was used as an example to analyse the current situation of management in AGRs and explore the construction and application of adaptive management model.海洋公益性行业科研专项(200905019;201005012

Construction and identification of interference plasmid targeting on TNFAIP8

目的:构建并筛选出干扰效率最佳的TnfAIP8-SHrnA-P SIrEn-rETrO Q干扰质粒。方法:通过生物软件选择3个TnfAIP8基因干扰位点,构建干扰质粒并测序验证,将干扰质粒及对照质粒分别转染至A549细胞,通过rT-PCr、WESTErn blOT检测干扰效率。结果:经rT-PCr和WESTErn-blOT证实TnfAIP8-SHrnA-P SIrEn-rETrO Q干扰质粒能有效干扰并抑制细胞内TnfAIP8基因的表达,通过流式检测发现降低TnVAIP8表达可以提高细胞对A dr5SC fV诱导凋亡的敏感性。结论:成功构建和设计了对TnfAIP8基因具有显著干扰效率的干扰质粒,为进一步研究TnfAIP8基因的功能奠定了基础。Objective: To construct and screen the high efficiency interference plasmid of TFAIP8-shRNA-p SIRENRetro Q.Methods: Selected and synthesized three Target Sequence of TNFAIP8 shRNA1,TNFAIP8 shRNA2,TNFAIP8 shRNA3,and construct the TNFAIP8 interference plasmid.Transfection TNFAIP8-shRNA-p SIREN-Retro Q interference plasmid to A549 cells.Filter out the highest interference efficiency plasmid by detecting the mRNA and protein levels using RT-PCR and Western blot methods.Results: We successfully design and built three TNFAIP8-shRNA-p SIREN-Retro Q interference plasmids,and screen out the highest efficiency interference plasmid.Conclusion: Three interference plasmids targeting the TNFAIP8 gene have been constructed successfully and provide a useful tool for studying the function of TNFAIP8.国家自然科学基金项目(81272720); 福建省卫计委医学创新课题(2014-CXB-43); 厦门市科技计划项目(3502Z2083008)资