Functional Characterization of AtST39 in Stress Response and AtMPA Mediating Pollen Development

基因芯片显示番茄细菌性叶斑病原菌Pseudomonassyringaepv.tomatoDC3000(PstDC3000)，干旱、冷和盐等逆境处理及在开花关键基因lfy突变体中都能够上调AtST39（At3g46110）基因表达；AtMPA（At1g05577）在拟南芥单核花粉粒向三核花粉发育的过程中特异性表达。但是AtST39参与胁迫和AtMPA在介导花粉发育的分子机制知之甚少。我们主要对AtST39进行了探索研究，结果如下： （1）NCBI数据库显示YWC基因家族共有185个蛋白家族成员。经蛋白多序列比对，发现YWC蛋白家族含有三个高度保守的模体。生物信息学预测YWC蛋白家族成员可能受到...Microarray data reveals expression level of AtST39( At3g46110) is up-regulted by pathogens such as Pseudomonas syringae pv. tomato DC3000 (PstDC3000), cold , drought and salt treatment and in key regultors lfy which controls flowering; while AtMPA (At1g05577) is specifically expressed in the process of uninucleate microspore switching to tricellular. However,the molec-ular mechanism of two memeber...学位：理学硕士院系专业：生命科学学院_细胞生物学学号：2172009115219

Expression Analysis of a Stress Repressed Gene OsDSR4 from DUF966 Family and Generation of OsDSR4- overexpressing Transgenic Rice(Oryza sativa L. ssp. japonica)

OSdSr4基因是duf966基因家族中的一个未知功能基因,目前其生物学功能尚不清楚。本研究生物信息学分析显示,OSdSr4基因CdnA全长2 167 bP,包含一个1 149 bP的开放阅读框(Orf),编码382个氨基酸,推测的蛋白中包含一个高度保守的duf966结构域;表达模式分析表明,OSdSr4主要在水稻(OryzA SATIVA l.SSP.JAPOnICA)的茎节间和叶片中表达,干旱、高盐和低温等非生物胁迫明显抑制了OSd Sr4的表达,而脱落酸(AbSCISIC ACId,AbA)则显著诱导了它的表达;利用重叠延伸PCr方法成功克隆了OSdSr4,并将其转化进水稻中,获得了32株超表达转基因植株。分子鉴定结果表明,该基因已被整合进水稻基因组中,并在部分转基因植株中实现了超量表达。本实验为进一步开展OSdSr4基因的生物学功能研究提供了基础资料。OsDSR4 is a gene of unkown function in DUF966 gene family,and the function of DUF966 family genes have not been reported until now.In this study,the bioinformatic analysis showed that the cDNA of OsDSR4 had 2 167 bp containing an open reading frame(ORF) of 1 149 bp,and it encoded a putative protein of 372 amino acids with a highly conserved DUF966 domain.The gene expression profile analysis indicated that OsDSR4 was expressed mainly in internode and leaf blade of rice(Oryza sativa L.),and it was repressed markedly by drought,salt and cold stresses,and induced significantly by abscisic acid(ABA).OsDSR4 was cloned using overlap extension PCR,and the fusion construct containing OsDSR4 was introduced into rice(Oryza sativa L.ssp.japonica) by Agrobacterium- mediated transformation method.Thirty- two OsDSR4- overexpressing transgenic plants were obtained and identified by PCR and qRT-PCR,which was demonstated that OsDSR4 had been integrated into rice genome and was overexpressed in some positive transgenic plants.These results establish the foundation for further study of the precise function of OsDSR4.国家重点基础研究发展计划(973)前期研究专项(No.2012CB126312

Advanced Persistent Threats Detection Game with Expert System for Cloud

云计算系统是高级持续性威胁(advanced persistent; threats,APT)的重要攻击目标.自动化的APT检测器很难准确发现APT攻击,用专家系统对可疑行为进行二次检测可以减少检测错误.但是专家系; 统完成二次检测需要花费一段额外的时间,可能导致防御响应延迟,而且专家系统本身也会产生误判.在综合考虑APT检测器和专家系统的虚警率和漏报率的基础; 上,用博弈论方法讨论在云计算系统的APT检测和防御中,利用专家系统进行二次检测的必要性.设计了一个基于专家系统的APT检测方案,并提出一个ES-; APT检测博弈模型,推导其纳什均衡,据此研究了专家系统对云计算系统安全性能的改善作用.此外,当无法获得APT攻击模型时,提出了一种利用强化学习算; 法获取最优防御策略的方案.仿真结果表明:基于WoLF-PHC算法的动态ES-APT检测方案较之其他对照方案能够提高防御者的效用和云计算系统的安全; 性.Cloud computing systems are under threaten of advanced persistent; threats (APT). It is hard for an autonomous detector to discover APT; attacks accurately. The expert system (ES) can help to reduce detection; errors via double-checking suspicious behaviors. However, it takes an; extended period of time for the ES to recheck, which may lead to a; defense delay. Besides, the ES makes mistakes too. In this paper, we; discuss the necessity of the ES to participate in APT detection and; defense for a cloud computing system by game theory, based on the; consideration of miss detection rates and false alarm rates of both the; APT detector and the ES. The ES-based APT detection method is designed,; and the ES-APT game between an APT attacker and a defender is; formulated. We derive its Nash equilibrium and analyze how the ES; enhances the security of the cloud computing system. Also, the dynamic; game is studied, in case that the APT attack model is unknowable. We; present a reinforcement learning scheme for the cloud computing system; with ES to get the optimal strategy. Simulation results show that, with; the knowledge of the ES, both the defender's utility and the cloud; computing system's security are improved compared with benchmark; schemes.国家重点研发计划项目; 国防基础科研计划项目; 国家自然科学基金项目; 北京市科委科技计划专项项目; CCF启明星辰鸿雁基金项

浮桥河水库浮游植物的多样性及其演变

1997年4月至1998年1月对浮桥河水库浮游植物状况进行了调查,结果表明:该水库常见浮游植物约189种,隶属于8门92属,其中硅藻门25属64种,绿藻门39属79种,蓝藻门17属30种,甲藻门、隐藻门、裸藻门、黄藻门及金藻门种类较少。其个体密度年平均为44.9×104ind/L;生物量年平均为6.439mg/L,其中蓝藻占33.37%,硅藻和绿藻分别占20.68%和20.16%,甲藻占16.31%。浮游植物现存量的季节变化表现为从春季开始平稳上升,至秋季达到最大值。蓝藻、硅藻、绿藻等主要种类的季节变化

浮桥河水库浮游植物的多样性及其演变

1997年4月至1998年1月对浮桥河水库浮游植物状况进行了调查，结果表明：该水库常见浮游植物约189种，隶属于8门92属，其中硅藻门25属64种，绿藻门39属79种，蓝藻门17属30种，甲藻门、隐藻门、裸藻门、黄藻门及金藻门种类较少。其个体密度年平均为44．9×10^4ind/L；生物量年平均为6.439mg/L，其中蓝藻占33.37％，硅藻和绿藻分别占20．68％和20．16％，甲藻占16．31％。浮游植物现存量的季节变化表现为从春季开始乎稳上升，至秋季达到最大值。蓝藻、硅藻、绿藻等主要种类的季节变化按生物量大小依次为：秋季夏季春季冬季。浮游植物现存量的水平分布为上游最高，中游次之，下游最低，库汊低于中游而略高于下游。群落的相似性表现为上、中游相近，下游和库汉相近。群落的物种多样性根据计算Shannon-Weiner指数为上游最大，其次为中游，下游再次，库汉最低。与1980年的调查结果相比较，浮桥河水库浮游植物种类小型化明显，生物量增加2．5倍

负载型贵金属催化剂硫中毒机理--不同载体的钯催化剂上硫的吸附及转化

以H 2 S为毒物, 用浸渍法制备了一系列不同载体的钯催化剂（Pd/Al 2 O 3 、Pd/MgO和Pd/TiO 2 ）以Na2S与催化剂机械混合的方式, 在载体上引入S2-,用H 2 —O 2 混合气对含硫的催化剂进行处理。藉XPS和IR谱对不同载体的催化剂上硫的吸附及转化进行了研究。结果表明, 载体酸碱性对其上担载的贵金属的电子状态有一定的影响。酸性较强的载体对贵金属的分散作用较强, H2S在这种载体的催化剂上的吸附和转化能力较差。在碱性较强的载体的催化剂上, 结果相反。此外, 载体上晶格氧的活性不同,对H 2 S的转化能力也有差异。在Pd/Al 2 O 3 催化剂运行过程中, H 2 S在Al 2 O 3 载体上主要发现为一种物理的吸附-脱附过程; 而在MgO和TiO 2 载体上, 不仅存在H 2 S的吸附过程, 且有H 2 S转化为SO 2 或SO 4 2- 的过程。结果还表明, 载体性质对催化剂的自身再生能力具有较大影响

负载型贵金属催化剂硫中毒机理--不同载体的钯催化剂上硫的吸附及转化

以H 2 S为毒物, 用浸渍法制备了一系列不同载体的钯催化剂（Pd/Al 2 O 3 、Pd/MgO和Pd/TiO 2 ）以Na2S与催化剂机械混合的方式, 在载体上引入S2-,用H 2 —O 2 混合气对含硫的催化剂进行处理。藉XPS和IR谱对不同载体的催化剂上硫的吸附及转化进行了研究。结果表明, 载体酸碱性对其上担载的贵金属的电子状态有一定的影响。酸性较强的载体对贵金属的分散作用较强, H2S在这种载体的催化剂上的吸附和转化能力较差。在碱性较强的载体的催化剂上, 结果相反。此外, 载体上晶格氧的活性不同,对H 2 S的转化能力也有差异。在Pd/Al 2 O 3 催化剂运行过程中, H 2 S在Al 2 O 3 载体上主要发现为一种物理的吸附-脱附过程; 而在MgO和TiO 2 载体上, 不仅存在H 2 S的吸附过程, 且有H 2 S转化为SO 2 或SO 4 2- 的过程。结果还表明, 载体性质对催化剂的自身再生能力具有较大影响

Kinetic model of induced codeposition of Ni-Mo alloys

The kinetic model of induced codeposition of nickel-molybdenum alloys from ammonium citrate solution was studied on rotating disk electrodes to predict the behavior of the electrode-position. The molybdate (MoO42-) could be firstly electrochemically reduced to MoO2, and subsequently undergoes a chemical reduction with atomic hydrogen previously adsorbed on the inducing metal nickel to form molybdenum in alloys. The kinetic equations were derived, and the kinetic parameters were obtained from a comparison of experimental results and the kinetic equations. The electrochemical rate constants for discharge of nickel, molybdenum and water could been expressed as k(1)(E) = 1. 23 x 10(-9) C(Ni)exp( - 0.198FE/RT) mol/(dm(2).s), k(2)(E) = 3.28 x 10(-10) C(Mo)exp(- 0.208FE/RT) mol/(dm(2).s) and k(3)(E) = 1.27 x 10(-6)exp(- 0.062FE/RT) mol/(dm(2).s), where C-Ni and C-Mo are the concentrations of the nickel ion and molybdate, respectively, and E is the applied potential vs. saturated calomel electrode (SCE). The codeposition process could be well simulated by this model

2002–2010年中国典型生态系统辐射及光能利用效率数据集

辐射是陆地生态系统能量的主要来源,其利用效率表现为光能利用率,反映了生态系统转化光能、生成有机物质的能力。揭示典型生态系统的辐射及光能利用效率可以为评估区域光能资源及其利用效率提供参考,也为评估区域有机物质固定能力及碳吸收能力提供依据。基于中国陆地生态系统通量观测研究联盟(China FLUX)的长期观测结果及已发表文献的公开数据,构建了2002–2010年中国典型生态系统辐射及光能利用效率数据集,包含51个生态系统126个站点年辐射、光能利用效率及吸收光能利用效率的观测记录。另外,本数据集还包含生态系统代码、年份、经度、纬度、海拔、生态系统类型、年均气温、年总降水量、年均CO2质量浓度、年均叶面积指数、最大叶面积指数等生物气候信息。本数据集可以为评估生态系统生产能力、应对气候变化等方面的研究提供数据支持

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel