一种多层特征融合的人脸检测方法

由于姿态、光照、尺度等原因,卷积神经网络需要学习出具有强判别力的特征才能应对复杂场景下的人脸检测问题。受卷积神经网络中特定特征层感受野大小限制,单独一层的特征无法应对多姿态多尺度的人脸,为此提出了串联不同大小感受野的多层特征融合方法用于检测多元化的人脸;同时,通过引入加权降低得分的方法,改进了目前常用的非极大值抑制算法,用于处理由于遮挡造成的相邻人脸的漏检问题。在FDDB和WiderFace两个数据集上的实验结果显示,文中提出的多层特征融合方法能显著提升检测结果,改进后的非极大值抑制算法能够提升相邻人脸之间的检测准确率。国家自然科学基金项目(61572409,61402386,81230087,61571188

化学信息学网络考试系统的构建

在WindowsNT 4 .0服务器建立化学信息学的Access试题数据库 ,使用ASP编写网络考试系统 ,供学生在Internet上使用浏览器进行在线考试。该系统安全可靠、应用范围广 ,是发展远程教育的重要工具

Establishment of MDCK cell lines which stably express visualable human neonatal Fc receptor

[目的]建立稳定表达融合EGFP的人新生儿Fc受体（h FcRn）的MDCK细胞株。[方法]构建重组慢病毒质粒p EGFP-h FcRn,采用四质粒包装系统共转染HEK 293T细胞生产重组慢病毒,感染MDCK细胞后对EGFP阳性细胞进行流式单细胞分选;通过Western Blot及EGFP-β2m荧光共定位验证h FcRn的完整性,并用流式细胞仪检测h FcRn与人Ig G的结合活性。[结果]测序结果表明成功构建p EGFP-FcRn慢病毒表达载体;感染后EGFP阳性MDCK细胞比例约为26.5%,流式单细胞分选后得到纯阳性细胞;荧光共定位及Western Blot均检测到h FcRn的完整表达;流式分析表明细胞株上的h FcRn与Ig G存在p H依赖性结合。[结论]成功获得稳定表达具有生物活性的可视化h FcRn的MDCK细胞株。[ Objective] To establish MDCK cell line stably expressing EGFP- human neonatal Fc receptor（hFcRn） fusion protein. [ Methods ] The lentiviral expression vector for EGFP - hFcRn fusion protein was constructed. Generating by co - transfection of four -plasmids into HEK 293T cells ,the lentivirus particles were used to infect MDCK cell line. EGFP positive single cell was obtained by FACS, and then FcRn expression was identified by fluorescence co -location with EGFP - β2m and confirmed by Western Blot. Flow cytometry was used to detect binding activity of hFcRn and human IgG. [ Results ] DNA se- quencing demonstrated that the lentivirus vector pEGFP - FcRn was constructed successfully. The percentage of EGFP - posi- tive ceils was about 26.5% after infection. Expression of the complete protein was detected through fluorescence co - location and Western Blot, respectively. Flow cytometry analysis showed that the cell lines could pH - dependently capture human IgG. [ Conclusion] MDCK cell line stably expressing functional visualable hFcRn was established.基金项目：国家自然科学基金项目（“结构生物学指导的HBV治疗性抗体人源化及其关键技术研究”,No.31600748;“抗呼吸道合胞病毒高中和活性抗体的保护机制研究”,No.81401668;“基于广谱中和单抗的通用型流感疫苗设计及其结构基础研究”,No.31670934

Experimental study on effect of Changrun Formula in regulating expression of AQP3 and AQP9 in colon mucosa of functional constipation rats

目的:观察肠润方对功能性便秘模型大鼠结肠黏膜AQP3、AQP9表达的影响,探究其调控AQP3、AQP9表达的分子机制。方法:60只SD大鼠随机分为空白组、模型组、阳性对照组(麻仁丸组)、肠润方组、肠润方联合P38MAPK抑制剂SB203580组。用复方地芬诺酯制造大鼠便秘模型,采用免疫组织化学及实时荧光定量PCR(q RT-PCR)检测大鼠便秘模型近端及远端结肠黏膜AQP3、AQP9的表达;Western Blot检测信号传导通路相关分子P38MAPK及P-P38MAPK的表达。结果:造模成功后,模型组近端结肠黏膜AQP3表达较空白组明显上升,而远端结肠黏膜AQP9表达较空白组明显下降(t值分别为3.148和7.069,P值均0.05)。肠润方联合P38MAPK抑制剂SB203580治疗后,近端结肠黏膜AQP3 m RNA水平显著上升(t=5.922,P0.05). The level of AQP3 m RNA in proximal colonic mucosa was significantly increased(t=5.922, P<0.01), and AQP9 m RNA was significantly decreased in distal colonic mucosa(t=4.038, P<0.01); The relative expression of P-P38/P38 protein was significantly decreased(t=19.419, P<0.01). Conclusion: The therapeutic action ofChangrun Formula on functional constipation rat models might through inhibiting the expression of AQP3 in proximal colon, and accelerating on expression of AQP9 in distal colon, and the regulating mechanism might relate with the inhibition on P38 MAPK and AKT phosphorylation.福建省自然科学基金项目(No.2012D043)~

Tannin Content of Different Casuarina Species and Its Distribution Law

探讨了几种木麻黄单宁含量与种类、年龄、部位、生态环境的关系。结果表明:8种木麻黄小枝总多酚含量排序为:细枝木麻黄(CASuArInA CunnIngHAMInA)>山地木麻黄(C.JungHuHnIAnA)>山神木麻黄(C.COllIA)>短枝木麻黄(C.EQuISETIfOlIA)>鸡冠木麻黄(C.CrISTATA)>粗枝木麻黄(C.glAuCA)>肥木木麻黄(C.ObESA)>滨海木麻黄(AllOCASuArI-nA lITTOrAlIS);不同年龄木麻黄小枝总多酚含量随着年龄的增加而增大。短枝木麻黄和细枝木麻黄总多酚、可溶性单宁、蛋白质结合缩合单宁以及纤维素结合缩合单宁含量大体上以细根>树皮>小枝。随着离海岸带距离的增加,木麻黄小枝总多酚含量降低。木麻黄小枝蛋白质结合态缩合单宁含量非常低,而纤维素结合态缩合单宁含量较高,这可能与木麻黄鳞片叶退化为小枝,纤维素含量高有关。Tannin contents of different Casuarina species and tannin distribution rules of species,ages,position and ecological environment were studied.The results showed that the total phenol of branchlet of Casuarina was under the order as follows:Casuarina cunninghamina>C.junghuhniana>C.collia>C.equisetifolia>C.cristata>C.glauca>C.obesa>Allocasuarina littoralis.The total phenol contents of branchlets of different years old of Casuarina increased with ages.The total phenol,solubility tannins,protein binding condensing tannins,fibrin binding condensing tannins contents of short and thin Casuarina branchlets were under the following order wholly:thin root>bark>branchlet.With the increasing of the distance from coastal belt,the total phenol of branchlet declined.The protein binding condensing tannin of Casuarina branchlet was very low,whereas fibrin binding condenseing tannins content was highest.The reason could be the high fibrin content after Casuarina scale leaf degenerated to branchlet国家“十一五”科技支撑计划(2006BAD01A1605);福建省林业厅科研项目“木麻黄单宁生化活性与提取利用技术研究”资

中国海及邻近区域碳库与通量综合分析

中国海总面积约470万平方公里,纵跨热带、亚热带、温带、北温带等多个气候带.其中,南海北依\"世界第三极\"青藏高原、南邻\"全球气候引擎\"西太平洋暖池,东海拥有全球最宽的陆架之一,跨陆架物质运输显著,黄海是冷暖流交汇区域,渤海则是受人类活动高度影响的内湾浅海.中国海内有长江、黄河、珠江等大河输入,外邻全球两大西边界流之一的黑潮.这些鲜明的特色赋予了中国海碳储库和通量研究的典型代表意义.文章从不同海区（渤海、黄海、东海、南海）、不同界面（陆-海、海-气、水柱-沉积物、边缘海-大洋等）,以及不同生态系统（红树林、盐沼湿地、海草床、海藻养殖、珊瑚礁、水柱生态系统等）多层面对海洋碳库与通量进行了较系统地综合分析,初步估算了各个碳库的储量与不同碳库间的通量.就海气通量而言,渤海向大气中释放CO2约0.22Tg Ca-1,黄海吸收CO2约1.15Tg Ca-1,东海吸收CO2约6.92～23.30Tg Ca-1,南海释放CO2约13.86～33.60Tg Ca-1.如果仅考虑海-气界面的CO2交换,中国海总体上是大气CO2的\"源\",净释放量约6.01～9.33Tg Ca-1.这主要是由于河流输入以及邻近大洋输入所致.河流输入渤黄海、东海、南海的溶解无机碳（DIC）分别为5.04、14.60和40.14Tg Ca-1,而邻近大洋输入DIC更是高达144.81Tg Ca-1,远超中国海向大气释放的碳量.渤海、黄海、东海、南海的沉积有机碳通量分别为2.00、3.60、7.40、7.49Tg Ca-1.东海和南海向邻近大洋输送有机碳通量分别为15.25～36.70和43.39Tg Ca-1.就生态系统而言,中国沿海红树林、盐沼湿地、海草床有机碳埋藏通量为0.36Tg Ca-1,海草床溶解有机碳（DOC）输出通量为0.59Tg Ca-1;中国近海海藻养殖移出碳通量0.68Tg Ca-1,沉积和DOC释放通量分别为0.14和0.82Tg Ca-1.总计,中国海有机碳年输出通量为81.72～103.17Tg Ca-1.中国海的有机碳输出以DOC形式为主,东海向邻近大洋输出的DOC通量约15.00～35.00Tg Ca-1,南海输出约31.39Tg Ca-1.综上,尽管从海-气通量看中国海是大气CO2的\"源\",但考虑了河流、大洋输入、沉积输出以及微型生物碳泵（DOC转化输出）作用后,中国海是重要的储碳区.需要指出的是,文章数据是基于中国海各海区碳循环研究报道,鉴于不同研究方法上的差异,所得数据难免有一定的误差范围,亟待将来统一方法标准下的更多深入研究和分析.国家重点研发计划项目(编号:2016YFA0601400);;国家自然科学基金项目(批准号:91751207、91428308、41722603、41606153、41422603);;中央高校基础研究项目(编号:20720170107);;中海油项目(编号:CNOOC-KJ125FZDXM00TJ001-2014、CNOOCKJ125FZDXM00ZJ001-2014)资

Research on Traffic Density Estimation and Vehicles Detection by Using Convolutional Neural Networks

智能交通系统作为智慧城市的重要组成部分，对缓解现代城市当中的交通拥堵状况和提高通行效率有着非常积极的推进作用。随着技术的发展和硬件设备的提高，目前的智能交通系统越来越依赖于来自于不同传感器的数据，其中基于计算机视觉的视频监控系统更是被广泛的应用于智能交通分析中。 分析交通场景中的交通拥挤程度对于交管部门重新规划车辆行驶路线和减少拥堵是十分重要的；同时，车辆检测是许多交通监控系统(如车辆计数、跟踪和检索等)的关键基础步骤。目前的针对交通场景的分析主要采用的是背景建模、光流、动态纹理等方法；车辆的检测算法以基于人工特征的方法为主，主要处理小规模的交通卡口摄像头的监控数据。 当前，交通视频分析领...Intelligent Transportation System is one of the core components of Smart City, and can do significant contributions for alleviating the traffic congestion and help improving traffic efficiency. With improvement of technology and hardware, traffic analysis is relying on data collected from various traffic sensors nowadays. Among various traffic surveillance techniques, computer vision based video s...学位：工学博士院系专业：信息科学与技术学院_计算机科学与技术学号：3152013015428

The Establishment and Technical Approach of a Mirror System of Chemical Information

[中文文摘]以 Teleport Pro离线浏览器作为镜像工具 ,下载 Internet上丰富的化学信息资源 ,建立了物理化学与应用化学信息资源镜像系统 ,并对镜像过程出现的有关技术和解决方法进行深入的探讨。[英文文摘]The mirror system for the physical and applied chemistry has been established with off line browser. The developing procedure of the mirror system was explained. A lot of information was reorganized and hyperlinked for CERNET users with the latest Internet technique. The technical aspect for the mirror operations was discussed in details.中国教育科研计算机网络物理化学与应用化学站点镜像子专题资助项

The Developing Technologies of Series Network Courses on Chemistry

[中文文摘]以化学网络课程为实例 ,介绍了网络教程的动画、音频、视频等方面的制作技术 ,并讨论了网络课程的多媒体制作工具。[英文文摘]Based on the series network courses of chemistry, the developing technologies of the animations, sounds and videos were introduced. Some multimedia softwares for developing network courses were discussed also