Isolation and identification of PAH-degradating strains and their degradation capability

以芴、菲、蒽、芘为碳源和能源筛选、分离PAHs降解菌。14株能降解利用PAHs的菌株被分离。通过HPLC分析,在含芴、菲、蒽、芘的混合培养基质中10号菌的降解能力最强。研究它的降解性能和生长情况,表明该菌在混合反应体系中培养30d后对芴、菲、蒽、芘的降解率分别为95.27、90.46、28和80%;在只含一种PAH的单反应体系中该菌对芴、菲、蒽的降解能力提高,降解率分别可达98.91、94.32和52.17%,而对芘的降解能力则降低,降解率仅为62.47%。与混合PAHs培养体系相比,在单一PAH培养体系中,细菌的对数生长期缩短1/3。经生理生化鉴定和16SrDNA序列对比分析,确定10号菌株属于假单胞菌,命名为PseudomonasspFAP10。Fluorenen, phenathalene, anthracene and pyrene were used as carbon and energey source to isolate and screen PAHs-degrading strains. Fourteen strains capable of degrading PAHs were isolated from the petroleum in seawater contaminated. Analyses was done by HPLC, strain No.10 showed the strongest degrading ability in the mixing system of fluorene、phenathalene、anthracene and pyrene. Its degradation performance and growth curve were tested. The degradation rates of fluorenen, phenathalene, anthracene and pyrene were 95.27%、90.46%、28% and 80% respectively in the mixing system within 30d. Under the condition of single PAH existence,the degradation rates of fluorene、phenathalene and anthracene by strain No.10 increased to 98.91%、94.32%、52.17% respectively while the degradation rate of pyrene decreased to 62.47%. Compared to the mixing system, the logarithmic growth phase of strain No.10 was shortened one third in single PAH system.. Based on the results of physio-biochemical identification and phylogenetical analyses of 16S rDNA sequence, strain No.10 was belonged to Pseudomonas sp,named as Pseudomonas sp FAP10.国家海洋局第三海洋研究所海洋生物工程重点实验室开放基金[A97304(021)

地域連携活動の意義について―「ビビッと！ 249～おいしい食と健康のフェスタ～」活動を通して―

In recent years Japan, many colleges have programed collaborative activities with local communities into the curriculum. We have discussed the educational effects that can be expected from the joint activities with the local community based on our college cases. There, it was deduced to be effective for own sociability with social norms, contribution to the community (awareness of a cooperation), self-esteem, and self-reliance, as a pre-stage of their professional activities. In this paper, we summarized the collaborative activity with local communities of “Vivid! 249, Delicious Food and Health Festa, 2019” which was carried out as collaborative learning with other course students, speculated the educational effects from observation of the activities, and the meaning of the activity was considered

Observation results by the TAMA300 detector on gravitational wave bursts from stellar-core collapses

We present data-analysis schemes and results of observations with the TAMA300 gravitational-wave detector, targeting burst signals from stellar-core collapse events. In analyses for burst gravitational waves, the detection and fake-reduction schemes are different from well-investigated ones for a chirp-wave analysis, because precise waveform templates are not available. We used an excess-power filter for the extraction of gravitational-wave candidates, and developed two methods for the reduction of fake events caused by non-stationary noises of the detector. These analysis schemes were applied to real data from the TAMA300 interferometric gravitational wave detector. As a result, fake events were reduced by a factor of about 1000 in the best cases. The resultant event candidates were interpreted from an astronomical viewpoint. We set an upper limit of 2.2x10^3 events/sec on the burst gravitational-wave event rate in our Galaxy with a confidence level of 90%. This work sets a milestone and prospects on the search for burst gravitational waves, by establishing an analysis scheme for the observation data from an interferometric gravitational wave detector

微囊藻和栅藻共培养实验及其竞争参数的计算

以Ｌｏｔｋａ－Ｖｏｌｔｅｒｒａ 的双种竞争模型为基础，进行试验设计。共培养试验中，两种藻类的增长行为是不同的，培养前期共培养中栅藻的数量大于纯培养中栅藻的数量，而在后期则相反，微囊藻则是在整个共培养过程中的数量都小于纯培养中的数量。通过纯培养取得参数Ｋ 和ｒ，变模型的微分形式为差分形式，以生长曲线拐点（密度制约起始点）出现的时间作为计算竞争参数的起始时间。经模拟计算获得竞争参数，表明微囊藻对栅藻的抑制能力是栅藻对微囊藻抑制能力的７倍。并以此作为微囊藻可能爆发成为“水华”的理由之一。应用所得的参数，拟合共

微囊藻栅藻资源竞争的动力学过程Ⅰ.光能和磷营养的半饱和参数及其生长率动态

以Tilman提出的“资源竞争机制学说”为依据 ,在单因子 (光、磷 )限制条件下 ,测定并计算铜绿微囊藻 (Microcystisaeruginosa)和斜生栅藻 (Scenedesmusobliquus)的半饱和常数 ,以双因子交互作用模型预测它们的生长动态 .结果表明 :在光强为 10 .0～ 17.1μE和磷浓度为 3.10～ 2 0 .0 μmol的范围内 ,微囊藻的生长率大于栅藻的生长率 .说明磷的增加是微囊藻成为水华的充分条件 ,但不是必要条件 ,至少低光强是一个重要的作用因子

微囊藻栅藻资源竞争的动力学过程Ⅰ.光能和磷营养的半饱和参数及其生长率动态

以Tilman提出的“资源竞争机制学说”为依据 ,在单因子 (光、磷 )限制条件下 ,测定并计算铜绿微囊藻 (Microcystisaeruginosa)和斜生栅藻 (Scenedesmusobliquus)的半饱和常数 ,以双因子交互作用模型预测它们的生长动态 .结果表明 :在光强为 10 .0～ 17.1μE和磷浓度为 3.10～ 2 0 .0 μmol的范围内 ,微囊藻的生长率大于栅藻的生长率 .说明磷的增加是微囊藻成为水华的充分条件 ,但不是必要条件 ,至少低光强是一个重要的作用因子

一种基于低压智能开关的配网台区自动化保护方法

本发明公开了一种基于低压智能开关的配网台区自动化保护方法，包括如下步骤：步骤1：在台区内各级开关位置设置断路器，并且每个断路器均配置保护装置，构成低压智能开关装置；步骤2：以每一台低压智能开关装置为最小单元，以每个配变台区为自治区域，分层分级划分保护区域；步骤3：根据划分的区域信息结合本地信息制定分布式保护策略，并且根据分布式保护策略建立分布式智能保护算法模型，实现故障的检测、定位与隔离；步骤4：对制定的分布式智能保护算法模型进行训练。本发明分区域独立管控，实现快速、有选择的隔离故障，停电范围更小、恢复供电更快

Effect of CH_2Cl_2 on the Redox Behavior of Ferrocene in Ionic Liquid BmimPF6

应用循环伏安和交流阻抗法研究有机溶剂二氯甲烷对二茂铁在离子液体1-丁基-3-甲基咪唑六氟磷酸盐(Bm imPF6)中电化学行为的影响.实验表明,二氯甲烷可促进离子液体的离子解离,减小离子液体粘度,增加离子液体电导率,加速二茂铁在离子液体中的扩散,增大氧化还原峰电流.由于电极界面双电层结构的变化,导致双电层电容增大,电极反应电阻减小,从而加速了界面电子传递反应.The effects of CH2Cl2 on the room temperature redox behaviors of ferrocene in ionic liquid,BmimPF6,were studied by cyclic voltammetry and AC Impedance.The results suggested that the presence of CH2Cl2,accelerated the dissociation of ionic liquid,leading to lower viscosity and larger ionic conductovity,hence,the diffusions of ferrocence in BmimPF6 were enhanced and the peak currents of redox reactions were increased.The changes in the double-layer structure at the electrode interface resulted in larger capacitance,which reduced the electrode recution resistances,accordirgly,the reaction kinetics of ferrocene were enhanced.作者联系地址：上海师范大学化学系;Author's Address: Department of Chemistry,Shanghai Normal University,Shanghai 200234,Chin