赤外・可視光による人体検出無線ネットワークの研究

電気通信大学201

Marketing Strategy of Corporate Deposit of A Bank, Fujian Branch

1914年，A银行在福建设立机构。经过百年发展，A银行福建分行在福建省金融业界积累了深厚底蕴，业务发展水平较高。但从2014年以来，随着我国经济下行，A银行福建分行的经营情况出现了一定程度的下滑。目前来看，在经历了30多年的飞速发展之后，我国旧有发展模式的弊病逐步累积：首先是产能绝对过剩和基建相对超前，导致财政刺激政策效用递减；其次是近年货币政策过于激进导致资产价格泡沫和通胀压力并存，导致货币政策的可操作空间受限；第三是随着美元进入加息通道以及特朗普当选的政策影响，都将对我国外贸环境带来持续压力。虽然2016年我国经济有企稳回升之势，但深层次的发展模式转变并未完成，未来几年将是中国经济相对困难...In 1914, A Bank set up the first branch in Fujian. After a hundred years of development, A Bank of Fujian Province has built a stable foundation and established a high level of business development in the financial industry of Fujian Province. However, with China's economic recession, there has been a certain degree of decline in the banking operation since 2014. At present, after more than 30 yea...学位：工商管理硕士院系专业：管理学院_工商管理硕士(工商管理硕士)学号：1792010115066

通信使・燕行使と近世東アジア : 呉允謙と黃㦿を中心に

2015-02-28departmental bulletin pape

通信使・燕行使と近世日本

名古屋大学博士(歴史学)doctoral thesi

Damage to Alexandrlum tamarense DNA Caused by Hydroxyl Radicals

利用羟基自由基(·OH)压载水处理系统,采用大气压强电场放电技术制取·OH溶液对塔玛亚历山大藻(Alexandrlum tamarense)进行处理。通过普通光学显微镜,荧光显微镜和电子显微镜对·OH处理前后的塔玛亚历山大藻的细胞结构进行观测。结果表明,·OH能有效破坏藻细胞,从而造成藻类死亡。利用随机扩增多态性DNA(random amplification polymorphic DNA,RAPD)和实时定量PCR(RT-PCR)相结合的技术检测·OH对DNA链的破坏作用。共得到了3条有显著差异的扩增产物。这3条扩增产物经测序,并通过NCBI(national center of biotechnology information)的比对分析,最终得到1条可用RT-PCR检测·OH对DNA破坏作用的基因序列。以上的结果表明,·OH压载水处理系统能有效去除塔玛亚历山大藻,并对其DNA造成破坏。This paper referred to a ballast water treatment system by using hydroxyl radical(·OH),in which the atmospheric pressure electric discharge technology was applied to produce hydroxyl radicals to treat Alexandrlum tamarense.The morphology of A.tamarense before and after being treated by ·OH was observed by ordinary optical microscope,fluorescence microscope and electron microscope,respectively.The findings presented that ·OH effectively did damage to the algal cells and led to algae perishing and the damage of ·OH to DNA strand was detected by using combination of random amplified polymorphic DNA(RAPD)and RT-PCR technology.In addition,three DNA bands with differences were found and sequenced;then sequence alignment was performed in the website of National Center of Biotechnology Information(NCBI).Finally,a gene sequence was found for RT-PCR analysis that further proved the damage to DNA strand.In conclusion,the ballast water treatment system of ·OH was capable to removes A.tamarense in water effectively,causing damages of the DNA strand.中国杰出青年学者基金(NSFC)(61025001);; 中国国家科技支撑项目(2013BAC06B00);; 海洋科学研究公共利益的专项基

A级数据中心综合能源系统多目标优化设计和调度

采用分布式综合能源系统取代数据中心的传统供能系统,是降低数据中心运行费用的有效措施。考虑A级数据中心的供能可用度,设计了一种分布式综合能源系统,并基于混合整数非线性规划方法,建立了综合经济和环境效益的多目标优化设计和调度模型,选取ε-约束法对相互制约的多目标问题进行处理,在通用代数建模系统(GAMS)中建模并调用LINDOGLOBAL求解器进行优化求解。采用多维偏好分析线性规划决策法从非劣解集中选出最终解,并通过逼近于理想解的排序方法进行验证。算例结果表明,优化后的分布式综合能源系统能够保证A级数据中心的供能可用度,同时在降低年总费用和减排二氧化碳方面均具优势

风险投资对上市公司投融资行为影响的实证研究

本文研究风险投资机构对上市公司投融资行为的影响机制和作用效果,结果发现:风险投资的加入不仅可以抑制公司对自由现金流的过度投资,而且可以增加公司的短期有息债务融资和外部权益融资,并在一定程度上缓解因现金流短缺所导致的投资不足问题。进一步研究还发现不同特征的风险投资机构均可起到抑制自由现金流过度投资的作用,但只有高持股比例、高声誉、联合投资或非国有背景的风险投资机构才能够显著地改善外部融资环境,缓解现金短缺公司的投资不足问题。综合本文研究结果,作者认为企业上市后仍然可以利用风险投资机构的监督职能、声誉资源和融资关系网络来解决代理问题和信息不对称问题,进而促进企业投融资行为的规范化和理性化

Study on the corrosion effect of ballast tank caused by ballast water treatment of hydroxyl radical

针对羟基自由基(·OH)法处理船舶压载水过程中可能对压载舱造成腐蚀情况进行研究。在最大羟基处理浓度2.5 Mg/l条件下作用压载舱常用低碳钢、不锈钢及非金属材料,检测处理前后压舱水对压载舱材料的腐蚀影响。结果表明羟基法处理压载水系统在高效杀灭外来有害生物的过程中,对压载舱金属与非金属并不具有明显的腐蚀增强,这一结果满足IMO的技术要求。羟基法在快速处理船舶压载水的同时对于压载舱壁金属腐蚀而言是安全的。该研究结果对日后压载舱的防腐设计以及压载水处理装置在船舶上的推广应用具有重要的意义。The thesis presents the study of the situation that hydroxyl radical( ·OH) may cause the corrosion of ballast tank material in the process of ballast water treatment.Under the highest using concentration of hydroxyl radical,the materials,such as low-carbon steel,stainless steel,and Non-metallic,are commonly used in the ballast tank to evaluate the corrosion influence that ballast water makes on the materials of ballast tank in the pre-and post-treatment testing.The result showed that the corrosion effect of the hydroxyl radical on the metal and non-metal materials does not increase obviously when used in the system of ballast water treatment to effectively kill the exotic pests,which meets the requirements of IMO.It is safe for ballast tank wall metal to utilize the hydroxyl radical in the ballast water treatment.The study result holds a great significance for the anti-corrosion design of the ballast tank and the popularization as well as the application of this ballast water treatment system on board.国家高技术研究发展计划(2012AA062609); 国家杰出青年科学基金资助项目(61025001

实时荧光定量PCR检测原发性肝癌中PRL-2基因的表达

【目的】建立TaqMan实时荧光定量逆转录聚合酶链反应（RT-PCR）法检测肝细胞再生磷酸酯酶2（PRL-2）mRNA表达水平，并应用该法检测原发性肝细胞癌中PRL-2基因的表达。【方法】构建含PRL-2基因开放阅读框架的T载体．制作标准曲线。提取手术切除12例人肝癌、门静脉癌栓（PVTT）和癌旁组织总RNA并逆转录为cDNA。应用实时荧光定量PCR法观察人肝细胞癌组织和门静脉癌栓及癌旁组织中PRL-2的表达水平。【结果】线性检测范围达5个数量级，最低检测下限为1×10^2拷贝，最高检测上限为1×10^6拷贝。PRL-2在所有的门脉癌栓及10例肝癌组织表达，仅在4例癌旁组织中表达。门脉癌栓PRL-2mRNA表达水平显著高于肝癌及癌旁组织（P〈0．01），肝癌组织表达显著高于癌旁组织（P〈0．01）。【结论】实时荧光定量PCR可以准确定量测定PRL-2基因的表达：PRL-2基因在门脉癌栓的更高表达提示其在肝细胞癌的发生、转移中可能起重要作用

Preparation of monoclonal antibodies against 3D protein of EV71 based on HBc particles as expression vector

目的:预测肠道病毒71型(EV71)非结构蛋白3D的表位,以HBc蛋白为载体展示多肽,制备并鉴定抗EV71-3D的特异性单克隆抗体(mAb); 。方法:应用生物信息学方法分析预测出EV71; 3D蛋白亲水性和免疫原性指数较高的多肽片段,并运用HBc颗粒型蛋白载体展示肽段,构建多肽融合蛋白,免疫BALB/c雌鼠,通过杂交瘤技术和亲和层析; 技术制备和纯化抗EV71-3D蛋白的特异性mAb,用间接ELISA、ELISPOT、IFA和IHC对mAb的性质进行初步鉴定。结果:构建表达分别; 嵌合3D蛋白34~ 43位氨基酸残基、61~ 76位氨基酸残基、151~; 164位氨基酸残基的HBc重组蛋白,免疫并经过多轮克隆化筛选,获得抗EV71-3D单克隆抗体3E1,其亚类为IgG2a;免疫荧光试验、ELISP; OT法和免疫组织化学染色结果显示其可与EV71特异性结合。结论:成功制备可特异性识别EV71的单克隆抗体3E1,为病毒的检测及进一步研究3D蛋白; 的功能奠定了基础,同时还验证了生物信息学技术与HBc颗粒型载体展示多肽技术相结合可快速高效地制备单克隆抗体。Objective: To prepare and preliminarily identify the monoclonal; antibodies(mAbs) specifically against 3D protein of Enterovirus; 71(EV71),using bioinformatics to predict the epitopes of 3D,with HBc; protein as a carrier.Methods: Artificial screening of 3D protein epitope; sequences by bioinformatic method,inserted into the major immunodominant; region(MIR) area of Hepatitis B virus core protein(HBc),to construct the; recombinant protein.BALB/c mice were immunized with the recombinant; virus like particles(VLPs),to prepare the mAbs against 3D protein of; EV71.Affinity chromatography technology was used to purify the mAb.The; indirect ELISA,ELISPOT,immunofluorescence and immunohistochemistry; staining methods were used to identify the characteristic of the; mAb.Results: We displayed 3D(aa34-43),3D(aa61-76) and 3D(aa151-164); epitopes by constructing fusion protein using HBc VLPs as a vector,after; hybridization,one positive hybridoma cell line(3E1) was selected by; ELISA.The isotype of 3E1 was IgG2a.The results of immunofluorescence and; immunohistochemistry staining assay showed that the mAb 3E1 could; specifically recognize EV71.Conclusion: The prepared mAb 3E1 can; specifically recognizes the EV71,which laid the foundation for the; detection of virus and further study on 3D protein,and verified the; bioinformatics technology combined with HBc carrier displaying peptides; could prepare mAb quickly and efficiently.国家自然科学基金项