QTL Mapping, Epistasis and Environmental Effects Analysis for Rice Chalkiness Trait

水稻的垩白性状是当前限制中国稻米品质提升的最主要因素.研究垩白形成机理及遗传特性,将有利于提高育种中垩白性状的改良效率.本课题组先前构建了广陆矮; 4号/佳辐占重组自交系(GJ RIL)及遗传图谱.本研究连续2年在上杭县和龙海市两地共种植6季GJ; RILs,据各季垩白性状表型数据进行遗传分析,结合遗传图谱进行QTL定位、上位性分析和环境效应分析.遗传分析发现垩白粒率和垩白度呈偏态分布,推测; 垩白性状受主效基因与微效基因共同影响.QTL定位中,垩白粒率获得3个QTLs,qPGWC2、qPGWC4和qPGWC5,遗传贡献率分别为2.84; %、3.74%和14.09%;垩白度获得3个QTLs,qDEC1、qDEC4和qDEC5,遗传贡献率分别为2.96%、4.88%和7.79%.上; 位性分析中,垩白粒率和垩白度分别获得7对和5对上位性QTLs,贡献率为0.23%~3.55%.RM307~RM518区间内同时检测到垩白粒率和垩; 白度的QTLs,并参与了垩白粒率和垩白度的上位性互作.RM598~RM5140区间内也同时检测到垩白粒率和垩白度的QTLs,也参与了垩白度的上位; 性互作.环境效应分析发现,垩白度的3个QTLs及~eqDEC10和~eqDEC9这对上位性QTLs均与2010年早季龙海种植环境发生显著或极显著; 的互作效应.Rice (Oryza sativa L.) chalkiness is the most important limiting factor; for currently improving rice quality in China. Studying rice chalky; formation mechanism and hereditary character will be helpful for; increasing the efficiency of the improvement of chalky quality in; breeding. A Guanglu'ai No.4/Jiafuzhan recombinant inbred line(GJ RIL); was developed and its genetic map was constructed previously by our; research group. In this study, the genetic analysis of rice chalkiness; was carried out basing on the chalky phenotyping data of GJ RIL from a; total of 6 growth seasons of two different locations, Shanghang county; and Longhai city, in two consecutive years. Then the QTL mapping,; epistasis and environmental effects of chalkiness were studied by using; these phenotying data and the genetic map. The genetic analysis; indicated that percentage of grains with chalkiness (PGWC) and degree of; endosperm chalkiness (DEC) showed skewness distribution, suggesting that; chalkiness trait was affected by both major and minor genes together. In; QTL mapping, three PGWC QTLs, qPGWC2, qPGWC4 and qPGWC5, were detected,; which explained 2.84%, 3.74% and 14.09% of the genetic variation,; respectively. Three DEC QTLs, qDECl, qDEC4 and qDEC5, were mapped, which; explained 2.96%, 4.88% and 7.79% of the genetic variation, respectively.; In QTL epistasis analysis, 7 and 5 pairs of epistasis QTLs for PGWC and; DEC were identified respectively, and their contribution rates ranged; from 0.23% to 3.55%. The RM307~RM518 interval harbored the PGWC and DEC; QTLs, which were involved in epistatic interaction of PGWC and DEC; respectively. The RM598~RM5140 interval also harbored the PGWC and DEC; QTLs, which also participated in epistatic interaction of DEC.; Environmental effect analysis showed the three DEC QTLs (qDECl, qDEC4; and qDEC5) and a pair of epistatic QTLs between ~eqDEC10 and ~eqDEC9 all; exhibited significant or very closely significant interaction effects; under the environmental conditions in early season at Longhai city of; Fujian province in 2010.福建省中青年教师教育科研项目; 厦门大学中央高校科研基本业务

A High-efficiency and Low Cost Method of DNA Silver Staining in Non-denaturant Polyacrylamide Gel Electrophoresis——An Example Based on Rice

非变性聚丙烯酰胺凝胶电泳银染技术已广泛用于dnA片段检测。介绍一种本实验室改进的高效省本的非变性聚丙烯酰胺凝胶电泳银染方法,该法将固定和染色两步骤合并,整个银染过程只需16 MIn即可完成。同时,该法无需使用无水乙醇和冰乙酸,减少了硝酸银、氢氧化钠及甲醛的使用量。A method of silver staining in non-denaturant polyacrylamide gel electrophoresis has widely been used to detect DNA fragments.Here we developed a high-efficiency and low cost method of DNA silver staining in non-denaturant polyacrylamide gel electrophoresis.The whole new procedure of silver staining has combined two steps of fixation and staining into one step and can be completed within 16 min.Furthermore, the new procedure eliminates the use of ethanol and acetic acid and reduces the use amount of silver nitrate, sodium hydroxide and formaldehyde in silver steps.福建省自然科学基金计划项目(2012J01153); 福建省高校产学合作重大科技计划项目(2011N5013); 福建省科技重大专项(2013NZ0002-2

Analysis of Epistatic and QE Interaction Effects of QTLs for Grain Shape in Rice

本研究利用基于明恢86x佳辐占水稻重组自交系(rECOMbInAnT InbrEd lInE,rIl)构建的SSr遗传图谱,总标记数为131。联合两季的稻米粒长(gl)、粒宽(gW)、长宽比(l/W)表型数据,应用混合线性模型方法进行QTl定位,并作加性效应、上位效应以及加性QTl、上位性QTl与环境(QTl-by-EnVIrOnMEnT,QE)的互作效应分析。检测到粒长、粒宽和长宽比的加性效应QTlS分别为6个、4个和4个,贡献率分别为23.67%、21.41%和25.78%;检测到8对粒长的上位性QTlS,5对粒宽的上位性QTlS,2对长宽比的上位性QTlS,贡献率分别为16.75%、22.36%和7.55%;环境互作检测中,发现共有9个加性QTlS和7对上位性QTlS与环境发生了互作。结果表明,上位效应在粒形性状的遗传与加性效应一样起了重要作用,环境互作效应对粒形性状有一定的影响。A linkage map consisting of 131 markers was constructed by using a recombinant inbred line (RIL) population derived from the indica-indica rice cross Minghui86×Jiafuzhan.Quantitative trait loci (QTLs) was located with a mixed linear model (QTLmapper1.6) based on the grain shape parameters such as grain length (GL), grain width (GW) and length-width ratio (L/W), and made analysis of additive effects, epistatic, and the interaction effects of QTL-by-environment (QE).Six, four and four QTLs were detected to have significant additive effects for GL, GW and L/W, and the contribution rate were 23.67%、21.41% and 25.78%, respectively.Eight, five and two pairs of epistatic QTLs with significant additive-by-additive (AA) interaction effects were identified for three traits, and the contribution rate were 16.75%、22.36% and 7.55%, respectively.Significant QE interactions were found for nine additive QTLs and seven pairs of epistatic QTLs.The results indicated that the epistatic effects played an important role on the inheritance of grain shape, and the environmental factor had certain effects on the three traits.福建省自然科学基金项目(X0750053);厦门大学科技创新计划项目(XDKJCX20063004)共同资

Mapping QTLs for Rice Grain Shape with QTL×Environment Interactions and Epistatic Effects Analysis

利用广陆矮4号x佳辐占水稻重组自交系构建了SSr标记的遗传图谱.联合2007年和2008年获得的两组稻米粒长(gl)、粒宽(gW)、长宽比(l/W)数据应用混合线性模型方法进行QTl定位,并作加性效应、加性x加性上位互作效应以及加性QTl、上位性QTl与环境的互作效应分析.结果显示;(1)在加性效应分析中两个群体共检测到4个控制粒长的QTl,4个控制粒宽的QTl,5个控制长宽比的QTl,贡献率分别为13.81%、15.36%和16.29%.(2)在上位互作效应分析中两个群体共检测到2对控制粒长的互作QTl,1对控制粒宽的互作QTl,3对控制长宽比的互作QTl,贡献率分别为5.77%、2.59%和7.42%.(3)环境互作检测中,发现共有13个加性QTl和4对QTl的加性x加性上位性与环境产生了互作效应.结果表明,上位性效应和加性效应都影响稻米粒形遗传,QE互作效应也对粒形有着显著的影响.In this study,a recombinant inbred line (RIL) population derived from the indica-indica rice cross ‘Guangluai 4’בJiafuzhan’ was used in mapping of Quantitative trait loci (QTLs).Based on mixed linear model QTLmapper1.6,mapping was carried out for grain shape such as grain length (GL),grain width (GW) and length-width ratio (L/W) in rice in 2007 and 2008.QTLs were determined at the one-locus and two-locus levels,and QTL-by-environment (QE) interactions were analyzed.Four,four and five QTLs were detected to have significant additive effects for GL,GW and L/W,and the contribution rate were 13.81%,15.36% and 16.29%,respectively.Two,one and three pairs of epistatic QTLs with significant additive-by-additive (AA) interaction effects (epistatic effects) were identified for the three traits,and the contribution rate were 5.77%,2.59% and 7.42%,respectively.Significant QE interactions were found for thirteen QTLs with additive effects and four pairs of epistatic QTLs.The results indicated that the epistatic and additive effects played an important role on the inheritance of grain shape,and the environmental factor had significant effects on the three traits.国家863计划项目(2007AA10Z179);福建省科技计划重点项目(2008N0122);厦门大学科技创新项目(XDKJCX20063004

Major QTLs Mapping and Analysis for Rice Grain Chalkiness and Grain Shape Traits

本研究用珍佳b(佳辐占/珍汕97b//珍汕97b的回交重组自交系f11,即bC1f11)x珍汕97b的f2群体,对稻米粒长、粒宽、长宽比、粒厚和垩白粒率性状进行遗传分析与QTl定位。结果表明,粒宽、长宽比、粒厚和垩白粒率均属于由多基因控制的数量性状,而粒长受一个主效基因控制。共检测到13个控制糙米粒长、粒宽、长宽比、粒厚和垩白粒率的QTlS。其中,在第3号染色体着丝粒附近rM16-rM411区间同时控制粒长、粒宽、长宽比和粒厚性状,遗传贡献率分别为49.8%、12.6%、39.3%和5.3%;在第5号染色体着丝粒附近rM7118-rM3683区间同时控制垩白粒率、粒宽、长宽比和粒厚性状,遗传贡献率分别为43.9%、44.5%、28.0%和15.0%;同时,在rM169-rM289区间也同时控制垩白粒率、粒宽、长宽比和粒厚性状,但各性状的遗传贡献率均较rM7118-rM3683区间的小。The F2 population was derived from the cross ZS97B (Zhenshan97B)×ZJB (ZhenjiaB) which was the backcross recombinant inbred line from Jiafuzhan×ZS97B.Based on the F2 population, the genetic analysis and QTL mapping of grain appearance quality traits, included grain length, grain width, grain shape (grain length-width ratio), grain thickness and PGWC (percentage of grain with chalkiness), was conducted.Grain width, grain shape, grain thickness and PGWC, belonged to the quantitative trait inheritance.But grain length showed the polygenic inheritance with a major gene.13 QTL associated with grain appearance quality traits were detected in this study.RM16-RM411 located in the pericentromeric region of rice chromosome 3 could explain 49.8%, 39.3%, 12.6%, 5.3% of the phenotypic variation, respectively, for grain length, grain shape, grain width and grain thickness.RM7118-RM3683 located in the centromeric region of rice chromosome 5 was associated with PVE (phenotypic variation explained) of 43.9%, 44.5%, 28.0% and 15.0%, respectively, for PGWC, grain width, grain thickness and grain shape.In addition, another pleiotropic QTL located between RM169 and RM289 on rice chromosome 5 was associated with less PVE for PGWC, grain width, grain thickness and grain shape than RM7118-RM3683.福建省科技创新平台建设计划项目(2007N2005);福建省科技重大专项(2008NZ0001);福建省自然科学基金项目(2011J01249);中央高校基本科研业务费专项资金(2010121088);厦门市科技计划项目(3502Z20083003)共同资

应用调整的膳食平衡指数评价厦门市区成年居民膳食质量

目的利用调整的中国膳食平衡指数(dbI-07)综合评价厦门市城区18~65岁居民膳食质量,为开展营养干预提供依据。方法采用多阶段分层整群抽样方法于2010年10—12月对厦门市区6个社区居委会成年居民进行调查,采用连续3天24 H回顾法对其中的520人进行了膳食调查,采用dbI-07的评分方法评价厦门城区成年居民膳食质量水平。结果被调查的厦门市城区成年居民的膳食质量处于中度失衡状态,摄入不足和摄入过量并存;女性的摄入不足程度高于男性;各年龄段中,40~50岁人群摄入不足程度最高,摄入过量程度最低。结论被调查的厦门市城区成年居民水果、奶类及豆类摄入不足,肉类、食用油及盐类摄入过量;成年居民的主要膳食模式为A模式,且A模式反映了膳食中摄入不足与摄入过量的问题均较少,为最优模式。中央财政转移支付地方项

Breedine and Utilization of New Hybrid Rice Combination Tongyou 039

桐优039是泉州市农业科学研究所与福建神农大丰种业科技有限公司合作用自育的桐A与泉恢039配组而成的三系杂交水稻新品种，于2014年通过福建省品种审定。表现高产稳产、壮秆大穗、熟期转色好等优点。介绍了桐A、泉恢039、桐优039的选育过程，以及桐优039的性状表现、栽培技术和制种技术等。A new three-line hybrid rice combination Tongyou 039 was derived from the CMS line TongA and a restorer line Quanhui 039, developed by Quanzhou institute of agricultural Sciences and Fujian Province Prodigy farm and great harvest seeds Inc., and registered by the Crop Cultivar Registration Committee of Fujian province in 2014. It was characterized by high-yieldin ability, stocky stem and big panicle and good performance in ripe time d so on. This paper introduced breeding procedure of Tong A, Quanhui 039 and Tongyou 039, and main characteristics, cultivation and seed production techniques of Tongyou 039.福建省星火项目（2016S0015）;泉州市科技计划重点项目（2015N27

早熟优质籼稻三系不育系伍天A的选育

【目的】选育早熟优质三系不育系,为优质稻新品种选育奠定基础。【方法】2007年早季以泉5B/天丰B的F4代为父本,与不育系泉5A测交,经过多代回交和多年观察筛选,选育早熟优质三系不育系新品种。【结果】选育出的早熟优质不育系伍天A,于2019年通过福建省农作物品种审定委员会审定(闽审稻20190047),表现育性稳定,不育株率100%,不育度99.99%;异交率高,柱头总外露率64.77%;米质优,稻米的主要品质指标:整精米率66.0%、粒长6.7 mm、长宽比3.4、垩白粒率4%、垩白度0.6%、透明度1级、碱消值7.0级、胶稠度50 mm、直链淀粉含量23.8%,其中整精米率、垩白度、透明度、碱消值均达到NY/T593-2013《食用稻品种品质》一等食用籼稻品种品质标准,但胶稠度、直链淀粉含量等指标还不够理想,需在保持其优质早熟等优良特性的基础上,对品质性状进行改造提升;早熟,播始历期均在66 d左右,与天丰A相同;配合力强,伍天A与R713配组育成的三系稻新组合——伍天A/R713于2019年通过福建省农作物品种审定委员会审定(闽审稻20190035)。【结论】伍天A是一个育性稳定、异交率高、米质优、早熟、配合力强的三系不育系,可以配制出优质、多种熟期的三系杂交水稻新组合

Substituent effect on the assembly of coordination polymers containing isophthalic acid and its derivatives

通讯作者地址: Yang, SY (通讯作者), Xiamen Univ, Dept Chem, State Key Lab Phys Chem Solid Surface, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Dept Chem, State Key Lab Phys Chem Solid Surface, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 2. W Anhui Univ, Dept Chem, Luan 237012, Peoples R China 电子邮件地址: [email protected] coordination polymers [Zn4(H2O)(ip)4(py)6]n1, {[Zn2(hip)2(py)4]2·(py)}n2, [Zn(tbip)(py)2]n3 and [Mn(tbip)(py)2]n4 (H2ip = isophthalic acid, H2hip = 5-hydroxyisophthalic acid, H2tbip = 5-tert-butylisophthalic acid, py = pyridine), have been hydrothermally synthesized and characterized. The photoluminescent properties of 1–3 have also been investigated. For 1–3, as substituents change from H, OH to tert-butyl, the coordination numbers of Zn2+ ions decrease, and the dimensionalities of the structures of 1–3 decrease from 2D (4,4) net, 1D double stranded chain to 1D chain. For 3 and 4, as Mn2+ tends to have a higher coordination number as compared to Zn2+, the structure of 4 is a 2D (4,4) net while the structure of 3 is a 1D chain. The substituents on isophthalic acid influence the coordination environments of metal ions and the coordination modes of the carboxyls, and thus determine the structures of the coordination polymers. The coordination behavior of metal ions also affects the formation of the structures.National Natural Science Foundation of China 20471049 20721001 20725310 20673085 National Basic Research Program of China (973 Program) 2007CB815301 2007CB81530

Determination of Trace Metal Elements in Crude Oils by Microwave Digestion-Inductively Coupled Plasma-Mass Spectrometry Assisted with Organic Solvent

李景喜( 1980 － ) ，男，山东临沂市人，研究实习员，从事分析化学研究。E-mail: jxli@fio．org． cn。[中文摘要]建立了二氯甲烷溶剂辅助微波消解-电感耦合等离子体质谱法测定原油中V、Cr、Fe、Ni、Cu、Zn、Mo、Co、Pb等16种微量金属元素。研究表明,用CH2Cl2分散原油样品,以浓HNO3-H2O2为消解体系,采用CEM微波消解系统消解样品,各元素线性关系良好,相关系数≥0.9995;检出限可达ng/L;方法精密度较高,相对标准偏差(RSD,n=3)<5.0%;回收率为92%～110%。以w(Ni)/w(V)与w(Fe)/w(V)比值为变量参数对不同原油样品进行聚类分析,表明国内与国外不同地区原油样品中各金属元素含量差异较大。[英文文摘]A method for determination of V,Cr,Fe,Ni,Cu,Zn,Mo Co,Pb,etc in different crude oils was established by inductively coupled plasma-mass spectrometry assisted with organic solvent microwave digestion.The oil sample was digested by microwave using HNO3-H2O2 as oxidant after dispersing in organic solvent(CH2Cl2).The results showed that the detection limits of the method reached ng/L level for sixteen elements.The linear correlation of the method was preferable with the correlation coefficient of better than 0.9995． The recoveries of the method were in the range of 92% ～ 110% with the precision of less than 5.0% RSD (n=3)．The ratios of w(Ni)/w(V) and w(Fe)/w(V) were distinct in different crude oil samples from oversea and domestic，and the index can be used to the cluster analysis for differencing the crude oil samples．国家海洋局第一海洋研究所基本科研业务费专项资助(2010G23);海洋公益性行业科研专项资助(200705011);海洋溢油鉴别与损害评估技术重点实验室开放基金资助(200920