The effect of nurse initiated patient/family education strategy on people with schizophrenia in Beijing

2004-2005 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

The Accounting Information on Network

网络的兴起与介入是新经济时代的一个明显特征 ,网络化经营正在成为当今经济活动中不可或缺的要素之一。网络化经济涉及到企业的经营战略、竞争能力和管理水平等方面的系统工程 ,也涉及到对会计信息系统的再认识和再建设。本文分析了网络经济的兴起及对会计信息的影响 ,指出了会计信息必须适应网络经济的趋势和要处理的几个关系The rise and popularization of the network has become a major characteristic of the age of New Economy.E-business is now becoming an elementary constituent in the business operations.E-business is not only a comprehensive system of business strategy,competence and management abilities of companies,but also relates to the recognition and re-construction of the accounting information systems.This article mainly focuses on the analysis of the implications about assounting information systems with the development of E-business,with a conclusion on the trend of an adaptation of accounting information system and the relevant relations which should be considered

塔里木沙漠公路防护林生态工程的综合生态环境效应

为了探讨重大工程的生态环境效应,从风沙环境、土壤环境、小气候、生物多样性和地下水环境5个方面对塔里木沙漠公路防护林工程的综合生态环境效应进行了研究.结果表明:(1)防护林改变了近地表风沙运动过程,防沙体系内风速、输沙率降低幅度分别达64%~80%和87.45%~99.02%,风沙流结构、沙物质粒度、地表蚀积状况也发生了明显变化;(2)与自然流沙地相比,防护林土壤容重减小,全盐含量、总孔隙度和含水量增大,土壤肥力质量明显提高,其变化基本呈现"先快后慢"的规律;(3)防护林对于小环境改善作用明显,距地面6m范围内,林地不同高度的空气温度普遍低于自然流沙地,而空气湿度则高于自然流沙地,林地土壤温度也低于自然流沙地;(4)随着防护林内生境的变化,土壤微生物种类显著增加,但不同种类微生物的种群分布并不均匀;(5)目前防护林灌溉抽水对地下水位、地下水矿化度的影响不大,水位在厘米级范围波动,矿化度的变化幅度在±1g·L-1左右,且没有明显的变化趋势

连续陶瓷纤维的制备、结构、性能和应用:研究现状及发展方向

连续陶瓷纤维是纤维增强陶瓷基复合材料的增强体,对提高陶瓷基复合材料的强度和韧性起关键作用,高损伤容限和高强度陶瓷纤维是阻止裂纹扩展实现陶瓷基复合材料强韧化的保障。本文对碳化硅、氮化硅、氮化硼、氧化铝和氧化锆等几种陶瓷纤维的制备方法、结构、性能和应用等方面进行了全面的综述,指出了今后的发展方向,期望为未来陶瓷纤维的研究、开发及应用提供参考。国家高技术研究发展计划(863)(2003AA305630);;国家自然科学基金(51472144);;山东省科技重大专项(2015ZDZX11003);;山东省青年学者未来计划(2016WLJH27

Culture medium optimization for algicidal strain Y35 and preparation of algicidal bacterial agents

塔玛亚历山大藻是一种有毒甲藻,常引发赤潮,严重威胁海洋生态的稳定和人类的健康,细菌Deinococcus sp.Y35表现出对塔玛亚历山大藻的杀藻能力,为促进菌株生长、提高杀藻效果并方便保存,对菌株Y35培养条件进行优化,并制备杀藻菌剂.分别确定菌株Y35生长所需的最适氮源、碳源、无机盐,并确定其最适添加量,在优化的基础上完成冻干菌剂的制备和最适冻干保护剂的选择.菌株Y35生长的最适培养基成分是1.0%胰蛋白胨和0.5%酵母粉.在优化的培养基基础上对菌株Y35进行发酵,培养至对数期后进行冷冻干燥,制备杀藻菌剂.菌株Y35需要添加1.0 g/L的蔗糖作为冻干保护剂.杀藻菌剂的杀藻添加量为2.0 mg/m L.本研究可为下一步将细菌应用于赤潮治理奠定基础.Alexandrium tamarense is a toxic dinoflagellate,which causes harmful algal blooms(HABs),posing a serious threat on marine ecology and human health.Deinococcus sp.Y35 shows algicidal activity on A.tamarense.This research aimed to optimize its culture medium and improve the preparation of bacterial agents.We determined the optimum nitrogen source,carbon source and inorganic salts of strain Y35,then decided the optimum adding amount,prepared the freeze drying bacterial agents,and chose the optimum freeze-drying protective agent.The optimum culture medium composition of strain Y35 was found to be 1% tryptone and 0.5% yeast extracts.We needed to add 1.0 g/L sucrose as a protective agent for bacterial culture of strain Y35 before freeze-drying.The optimum amount of algicidal agent was 2.0 mg/m L.The optimization of culture conditions could ensure the bacterial growth and algicidal activity.The results of this research provide some foundation for HABs control in the future.国家自然科学基金项目(41576109,31500095);; 河南师范大学博士科研启动基金(5101049170160)资助~

SCANNING ELECTRON MICROSCOPICAL OBSERVATION ON ADULT NEOBENEDENIA MELLENI(MONOGENEA,CAPSALIDAE)

报道寄生于福建海水养殖鱼类高体的玫氏新本尼登虫 (单殖吸虫纲 ,分室科 )的扫描电镜观察。虫体体表无棘 ,前吸器和后吸器盘状。副甲片、前钩和后钩位于鞘内Neobenedenia melleni(MacCallum,1927) Yamaguti,1963,a capsalid monogenean parasitising the skin of marine cultured fish,Seriola dumerili(Carangidae) was studied by scanning electron microscope (SEM).The body surface of N.melleni lacks spines.Both the anterior attachment organs and haptor are disc shaped,the accessory sclerites,anterior hamuli and posterior hamuli are sheathed in tegument.Accessory sclerites possess sharp distal tips.Anterior hamuli and posterior hamuli possess curved tip,and marginal hooklets are arranged radially around haptor.ThisworkwassupportedbytheFujianProvincialNaturalScienceFoundation (B 991 0 0 0 5

Construction of eukaryotic vector pcDNA3.1-flag-pygo2 and expression in C6 cell

目的构建pcDNA3.1-flag-pygo2真核表达载体并在C6细胞中进行表达。方法从小鼠脑胶质细胞中提取总RNA,RT-PCR法反转录合成c DNA,设计引物,调取目的片段,与pcDNA3.1-flag载体连接后转化大肠杆菌DH5α,LB平板筛选菌落,提取质粒。重组质粒pcDNA 3.1-flag-pygo2经过酶切鉴定及测序后,阳离子脂质体法转染C6细胞并经免疫细胞化学染色及蛋白印迹检测重组体的表达。结果重构质粒pcDNA 3.1-flag-pygo2经限制性核酸内切酶EcoRⅠ,HindⅢ酶切分析及测序检查,表明真核表达载体构建正确;瞬时转染C6细胞后,免疫细胞荧光染色及蛋白印迹检测表明转染细胞能够表达外源Pygo2基因。结论成功构建了pcDNA3.1-flag-pygo2真核表达载体并能够在真核细胞中进行表达,这为今后研究pygo2基因在胶质瘤中的作用机制奠定了基础。Objective To construct the eukaryotic expression vector pcDNA3.1-flag-pygo2 and express the combined protein in C6 cell line.Methods To extract total RNA from primary glial cell of mouse and to synthesize c DNA by RT-PCR,then design primer and clone whole segment of pygo2 gene.After the targeted gene was inserted into vector pcDNA3.1-flag,the recombined plamid was transformed into E coli DH5α for LB agar plate screening and the recombined plasmid were extracted and purified.After verification by double enzyme digestion and sequencing.,the constructed eukaryotic expression plamid was transfected to C6 cell line by lipofectamin method,finally the protein expression was detected by immunocytochemical staining as well as western blot.Results The new constructed vector was confirmed by restricted enzyme Eco R I,HindIII digestion assay and correct Pygo2 was verified by sequenceing.finally,pcDNA3.1-flag-hpygo2 can express exogenous pygo2 gene in glioma C6 cell line after transient transfection by the determination of immunocytofluorescent staining and western blot.Conclusion The new plamid pcDNA3.1-flag-pygo2 was constructed successfully,and can express fused protein in eukaryotic cell,which establish the foundation for future research on pygo2 gene function in human glioma

A New Genus Record of Phyllobothriidae from Marine Fish Dasyatis akajei in China

报道检获于厦门海域赤魟(Dasyatis akajei)肠道中的四叶目叶槽科蔷薇属叶枕蔷薇线虫Rhodobothriumpulvinatum Linton 1889,经鉴定比较,为我国鱼类绦虫属的新记录。本次共剖检赤魟46尾,阳性2尾,感染率为4.3％,感染强度为5～7条/尾,并对所获虫体进行了描述。Rhodobothrium pulvinatum, one species of Phyllobothriidae (Cestoda: Tetraphyllidea) from marine fish Dasyatis akajei in Xiamen, Fujian, China was reported. The genus Rhodobothrium Linton, 1889 was erected for R. pulvinatum from the intestine of Dasyatis kuhli. which was new genus record in China, Dasyatis akajei was also a new record of host. 46 of Dasyatis akajei were examined, 2 of which were infected with cestode, the infective rate was 4. 3%, the infective intensity of the cestode was 5-7, the description of the worm is provided in the present paper.厦门大学校级自选课题资助(2002

人垂体腺瘤细胞原代培养的纯化及其激素的表达与分泌

【目的】探讨原代培养垂体腺瘤的细胞纯化方法及其激素表达与分泌的特点。【方法】采用三种原代培养方法对垂体腺瘤细胞进行培养，方法Ⅰ为目前常用的垂体瘤细胞培养方法，方法Ⅱ结合使用右旋颉氨酸（D-valineDMEMD-valine）培养液，方法Ⅲ采用反复贴壁法结合DMEMD-valine培养液培养。观察其细胞形态变化和生长特征，放射免疫法测定不同培养时间培养液中相应激素的水平。免疫组织化学染色法检测培养细胞生长激素（growthhormone，GH）、泌乳素（prolactin，PRL）的表达。【结果】3种方法均能成功培养出垂体腺瘤细胞，呈圆形或椭圆形，其纯度随培养时间的延长逐渐下降，培养第20天其平均纯度分别为40％，46％，96％。方法Ⅲ明显高于方法Ⅰ和方法Ⅱ，差异具有显著性意义（P〈0．01）；培养液中均含有相应激素。且3种方法的激素分泌量没有统计学差异（P〉0．05）；腺瘤细胞分别呈GH，PRL阳性表达，成纤维细胞表达Ⅰ型胶原。【结论】方法Ⅲ可得到纯度达95％以上的人垂体腺瘤细胞，纯化后的细胞可分别呈GH和PRL阳性表达并具有良好的激素分泌功能，为进一步研究人垂体腺瘤的发病机理、药物治疗和颅外移植等方面奠定了实验基础

舌鳞癌微血管密度与预后的关系

【目的】研究舌鳞状细胞癌(简称舌鳞癌, 下同)组织中新生微血管的密度(MVD)与舌鳞癌患者预后的关系。【方 法】用免疫组织化学S-P 法对75 例舌鳞癌标本进行染色, 依据Weidner 法进行肿瘤微血管密度计数, 并与舌鳞癌的病理分级、 颈淋巴结转移与否及患者生存期作比较。【结果】不同分化程度舌鳞癌组织中新生血管密度不同(P <0.01), 低分化者 M VD 高于高分化者;G1 , G2 , G3 期MVD 分别为24±10, 31 ±11 和48 ±12 ;颈淋巴结转移组MVD 为36 ±11 , 无颈淋巴结转移 组MVD 为27±9(t =2.383, P <0.01);五年生存组MVD 为26 ±9, 五年内死亡组MVD 为34±10(t =2.042, P <0.01)。 【结论】肿瘤微血管密度与舌鳞癌生物学行为相关, 可以作为舌鳞癌患者预后的指标