Arts in the Small Community: Essays and Supplements Celebrating the 50th Anniversary of Arts in the Small Community: A National Plan

The Arts in the Small Community: Essays and Supplements Celebrating the 50th Anniversary of The Arts in the Small Community: A National Plan. Eight essays and fourteen supplements from the original project provide the intellectual framework and the practical exploration of empowering arts in democratic America. The 1966 version is the original National Plan, while the 2006 version includes the stirring language of the original, but recognizes all of the dramatic progress in community arts development that took place between 1966 and 2006. It was intended to be a do-it-yourself manual to help local arts activists focus their work.https://engagedscholarship.csuohio.edu/msl_ae_ebooks/1002/thumbnail.jp

Cloning and sequence analysis of hepcidin-like cDNA Hepc2 from liver of Lateolabrax japonicus

Hepcidin(Hepc)是一类性质独特的抗菌肽,具有广谱的抗革兰氏阳性、阴性细菌和真菌等作用。利用RT-PCR和RACE等技术,从经过多种细菌攻毒的花鲈(Lateolabrax japonicus)肝组织中克隆出Hepc全长cDNA,命名为Hepc2,Genbank登录号为AY604195。Hepc2有581个碱基,其中阅读框有258个碱基,编码86个氨基酸。预测氨基酸序列和白鲈及其他鱼种在相同保守的区域有8个半胱氨酸,相对分子量为9418.55dal。在3’非编码区有225bp,包含终止密码子下游189nt处的多腺苷酸化AATAAA信号和212nt处的polyA信号。预测蛋白的信号肽断裂位点在第24和第25个密码子之间。通过与白鲈(Morone chrysops)、人和其他鱼种来源的Hepc cDNA和蛋白质同源性分析表明,从花鲈肝中分离的Hepc2 cDNA属于Hepc基因家族的新成员。Hepcidin is a unique antimicrobial peptide which exerts broad-spectrum antimicrobial action against Gram-negative and Gram-positive bacteria,as well as fungi.A Hepc-like cDNA was amplified from the liver of Lateolabrax japonicus challenged with a mixed bacteria solution.Using RT-PCR and RACE with a specific primer pair,a full length cDNA sequence Hepc2 of the Hepc-like antimicrobial peptide(GenBank accession number:AY604195) was obtained.Hepc2 cDNA is composed of 581 bases,which contains an ORF of 258 bases,encoding 86 amino acids.The deduced amino acid sequence is conserved between white bass and other fish species,which share eight cysteines at the identical conserved position.The relative molecular weight of the protein is 9418.55dal.The 3′non-coding region is composed of 225bp,with a polyadenylation signal AATAAA sequence appearing at position 189 nt,and poly(A) tail at 212 nt,downstream codon TAA.The signal peptide cleavage site of its deduced protein is presumed between codon 24 and 25.High homologies with Hepc cDNAs and proteins of white bass(Morone chrysops),human and other fish are shown.It indicates that Hepc2 cDNA from Lateolabrax japonicus liver is a new member of the Hepc gene family.福建省重大科技项目(2003I005);; 厦门市高新技术项目(3502Z20021052)资

假微型海链藻硅吸收及硅化作用与细胞周期偶合关系的研究

采用硅饥饿的培养方法,假微型海链藻(Thalassiosira pseudonana Hasleet Heimdal)得以同步生长.再通过流式细胞仪测定假微型海链藻的细胞周期,荧光显微镜观察假微型海链藻新硅质壁的形成,硅钼黄法检测假微型海链藻在细胞周期各阶段硅含量的变化,研究了假微型海链藻在细胞分裂周期中硅吸收与硅化作用的变化特征及相互间的偶联关系.结果表明:硅饥饿24h后,细胞停滞在G1/S期的边界,重新加入硅后,细胞同步生长.同步化培养后1h和3h,分别是吸收硅、合成新壳环带的主要时期,与G1/S期偶合;第5小时和第7小时,分别是吸收硅、合成新壳面的主要时期,与G2+M期偶合

Abundance and production of bacteria and their correlations with environmental factor

以2014年8月南海北部海水样品为研究对象,利用平板计数法和流式细胞仪计数法对南海北部表层和垂直海域可培养细菌和细菌总数分布状况进行研究,对细菌; 生产力进行测定,并结合环境因子进行相关性分析。结果表明:珠江口到南海北部海域,水平方向可培养细菌总数变化范围是3.70*10~2 ~; 1.42*10~3 CFU/mL,细菌总数变化范围是5.12 * 10~5 ~1.61 * 10~6; cells/mL,细菌生产力的变化范围是0.03 ~0.40 mg/m~3/h;垂直方向上可培养细菌变化范围是1.08 *10~3 ~9.00; *10~3 CFU/mL,细菌生产力变化范围是0.01 ~0.08; mg/m~3/h,其中表层海水中的细菌生产力明显高于底层。与环境因子相关性分析表明,水平方向上,影响南海北部表层海水细菌总数和细菌生产力的主要因; 素是温度、盐度、硝酸盐(NO_3-N)、硅酸盐(SiO_3-Si)、亚硝酸盐(NO_2-N)和磷酸盐(PO_4-P)(P<0.05);垂直方向上; ,影响南海北部可培养细菌总数的主要因素是NO_2-N(P<0.05),影响细菌生产力的主要影响因素是温度和盐度(P; <0.05)。可见,南海北部表层海水中细菌总数高于可培养细菌总数2; ~3个数量级,表明该海域表层海水存在大量不可培养细菌;细菌的生命活动在海水表层相较底层更为活跃。Distribution of abundance and production of bacteria and their; correlations with environmental factor were investigated, using plate; count method and flow cytometry, in the surface and vertical waters of; the northern south China sea in August 2014. The cultivable bacterial; abundance ranged from 3.70 * 10~2 CFU/mL to 1.42 * 10~3 CFU/ mL, the; total bacterial abundance ranged from 5.12 * 10~5 cells/mL to 1.61 *; 10~6 cells/mL and the bacterial productions varied from 0.03 mg/m~3/h to; 0.40 mg/m~3/h in horizontal distribution. In vertical distribution, the; cultivable bacterial abundance ranged from 1.08 * 10~3 CFU/mL to 9.00 *; 10~3 CFU/mL and the bacterial productions varied from 0.01 mg/m~3/h to; 0.08 mg/m~3/h, respectively. The correlation analysis results showed; that the environmental factors affecting the abundance of total bacteria; and bacterial productivity included temperature, salinity,; nitrate(NO_3-N),silicate (SiO_3-Si), nitrite (NO_2-N),and phosphate; (PO_4-P) (P<0.05). NO_2-N was the main influencing factor to cultivable; bacteria abundance (P < 0.05), while bacterial productivity was highly; correlated with temperature and salinity in vertical distribution of; northern south China sea (P < 0.05). The total bacterial abundance was 2; ~ 3 orders of magnitude higher than cultivable bacteria, suggesting that; there were many uncultured bacterium in surface water of northern south; China sea and bacterial activities in the surface maybe more active than; in the bottom.全球变化与海气交互作用; 国家重点研发计划全球变化及应对专项; 海洋公益性行业科研专

Diversity of CO_2 fixation gene in the surface waters of northern South China Sea in the Calvin cycle

为揭示南海北部表层海水中参与卡尔文循环的固碳基因多样性及其与环境因子的关系,本研究以卡尔文循环中的关键酶核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubis CO)的Ⅰ、Ⅱ型基因(cbbL、cbbM)作为分子标记,采用Illumina Miseq高通量测序技术对海水中的固碳基因多样性进行分析,并结合多元统计分析的方法,探讨了固碳基因多样性与环境因子的关系.结果显示,在南海北部表层海水中,含cbbL基因固碳基因主要归属于变形菌门(Proteobacteria)、蓝藻门(Cyanobacteria)和厚壁菌门(Firmicutes),其中,优势亚群分别是γ-变形菌亚门(45.3%)、蓝细菌(30.9%)和β-变形杆菌亚门(23.8%);而含cbbM基因的固碳菌群未检测到;近岸的A9站位与其他站位物种组成有一定的差异,异着色菌属(Allochromatium)、硫杆菌属(Thiobacillus)和硫单胞菌属(Thiohalomonas)为其特有菌属.相关性和冗余分析(RDA)结果显示,含cbbL基因的固碳基因丰度与水温、盐度呈显著负相关(p<0.01),与硝酸盐、亚硝酸盐、硅酸盐呈显著正相关(p<0.01).To elucidate the diversity of CO_2 fixation and its relationship with environmental factors in surface water of northern South China Sea,the coding gene form I( cbbL) and form II( cbbM)( that encodes Ribulose-1,5-biphosphate carboxylase/oxygenase( Rubis CO) were chosen as biomarkers in Calvin-Benson-Bassham cycle( a common carbon fixation pathway for chemolithoautotrophic microorganisms) to analyze the carbon sequestration gene diversity. The Illumina Miseq sequencing method and multivariate statistical analysis were employed. The cbbM gene was not detected in any of samples,while the cbbL gene was found in all samples. The cbbL-containing genetic communities were dominated by Proteobacteria,Cyanobacteria and Firmicutes,in which γ-Proteobacteria( 45. 3%),Cyanobacteria( 30. 9%),β-Proteobacteria( 23. 8%) were predominant subpopulation. There were differences between the nearshore station of A9 with other stations,in which Allochromatiu,Thiobacillus and Thiohalomonas were unique species in A9 station.Relativity and redundancy analysis suggest that water temperature, salinity, nitrate, nitrite, silicate were highly statistically significant factors.Temperature and salinity were positively( p < 0. 01),while the other three factors negatively influenced CO_2 fixation gene of cbbL gene abundance( p <0. 01).全球变化与海气交互作用专项(No.GASI-03-01-02-05);; 海洋公益性行业科研专项(No.201305030,201405007)~

黑龙江省扎赉特旗牛羊胰阔盘吸虫病流行病学及病原生物学的研究

一、前言 牛羊胰脏吸虫病是我国各农牧区中常见而且危害严重的一种吸虫病,国内此吸虫病的病原主要有胰阔盘吸虫(Eurytrema pancreaticum(Janson)、腔阔盘吸虫(E.coelomaticum(Giard et Billet)及枝睾阔盘吸虫(E.cladorchis Chin,Li et Wei)三种,其中以胰阔盘吸虫的分布区最大。关于本类吸虫的生物学问题近年来经国内外蠕虫学者的探讨而逐渐被了解(唐仲璋,1950;唐仲璋、唐崇惕,1975、1977;Basch,1965;Ksembaeva,1967;1973等)。国内在福建已详细观察了腔阔盘吸虫和枝睾阔盘吸虫生活史全过程,并经试验证实了它们的贝类宿主及不同的昆虫宿主的种类。胰阔盘吸虫的生活史虽亦经阐明,

应用“血防846”等药物驱除羊只胰阔盘吸虫的试验报告

家畜胰阔盘吸虫病广泛流行于世界各地。在我国主要分布于东北、内蒙、西北一带的广大农牧区;南方的福建、江西、浙江、广东、广西、贵州等省、区也有报道。 胰阔盘吸虫不仅寄生于羊、牛、猪、骆驼等家畜,而且也可感染人体,威胁人类健康。胰阔盘吸虫寄生于家畜的胰管中,由于机械性刺激、堵塞,以及代谢产物的作用,营养的夺取,引起胰脏的机能障碍和形态改变,如胰管的高度扩张,管壁增厚,粘膜出血、溃疡、炎性细胞的浸润,整个胰脏结缔组织增生,呈慢性增生性胰腺炎。从而使胰液腺和胰岛素的生成、分泌发生改变,全

用于半导体器件中的取样光栅的制作方法

一种用于半导体器件的取样光栅的制作方法，其特征在于，包括如下步骤:步骤1:在衬底上依次生长缓冲层、下波导层、多量子阱、上波导层，得到含有半导体波导结构的芯片;步骤2:在上波导层的上面，均匀地涂上一层光刻胶;步骤3:对涂有光刻胶的芯片进行全息曝光，使光刻胶上印制出条状的光栅轮廓;步骤4:将芯片置于取样周期光刻版之下进行二次曝光，二次曝光后对芯片上的光刻胶显影，使光刻胶形成有光栅轮廓的胶条;步骤5:对显影好的芯片，进行离子刻蚀，在上波导层上得到光栅;步骤6:用腐蚀液修整光栅形貌，完成取样光栅的制作。本发明具有成本低、适于大规模生产的优点

Design and Implementation of a Remote Automatic Meter Reading System Based on GSM

结合电力公司现代化发展要求,提出了一种基于GSM的远程自动抄表设计方案,并将研发产品投入使用。用单片机从数字电表等数据终端收集数据,并通过GSM模块实现数据的远程无线传输,最后通过数据交换平台将数据存入后台数据库中,此系统还开发了Web服务器,可供管理员和用户登陆网站管理和查询信息,实现了信息的现代化管理。For the modern development of electric company,a remote automatic meter reading system based on GSM is designed.And the products are realized and applied.In this system,automatic meter reading is completed by SCM and the remote wireless transmission is realized in GSM module.Finally,by using the Data Interchange Platform(DIP),data can be stored into the background database.Moreover,the system includes a Web server realizing the modern information management.On-line management and information query are supported for the administrators and customers respectively.福建省科技重点项目(2004H012