细胞培养系中的编程性细胞死亡

简要介绍了生物反应器中细胞培养系的编程性死亡和几种可能的控制细胞编程性死亡的途径

人口老龄化对我国产业结构调整与优化的影响

人口老龄化是经济社会发展不可逆转的大趋势 ,正对我国产业结构的调整与优化产生深刻的影响。它推动第三产业发展 ,促使劳动力的产业转移 ,直接促进产业结构调整与优化第一个任务的完成 ,但不利于其第二个任务的完成。促进产业结构调整与优化两个基本任务顺利完成 ,需要消除人口分布不合理和劳动力素质不高的双重制

The research of multi-class problem based gene expression profile data

针对基因表达谱微阵列的数据多分类问题,给出一种在多病类情况下的基于信噪比和相关性的特征基因选择方法.该方法一次性考虑基因区分所有病类的能力,尽量避免基因的冗余性;其次利用支持向量机,构建了基因表达谱微阵列数据的多分类器;最后通过实验表明了本方法的有效性.Aiming at the multi-class problem of gene expression profile data,this paper proposes a gene selection method based on S2N and correlation for multiple diseases.This method takes the classification abilities of genes to separate all the diseases into consideration at a time and tries to avoid redundancy in selected genes.Secondly,we construct multi-classifier of gene expression profile data using SVM.Finally,we do experiment by this method,the result of which shows great effectiveness of the method.国家自然科学基金资助项目(60704042

近地表上覆岩层直壁塌陷机理及稳定性分析

介绍了川口钨矿塌陷区岩层的地质条件、岩石的力学性质和工程地质调查与评价的结果;通过井下和地表现场调查和勘察测定,给出了井下采空区的边界范围和地表塌陷范围的对照图,分析了上覆岩层的塌陷特点,确定了采空区上覆岩层呈现典型的筒状直壁塌陷。根据采空区围岩和上覆岩层的节理裂隙发育程度不同,采用太沙基地压理论解释了采空区周边围岩和上覆岩层的力学作用机理和破坏特点,对太沙基理论进行了改进,并给出了力学分析图、推导了地压计算公式;进而采用极限破坏理论对不同开采跨度时上覆岩层的稳定性进行了分析和计算,并给出了在保证不产生筒形塌陷条件下、不同覆盖层厚度时的安全开采跨度和安全系数之间的量化关系

汇率决定与央行干预——1994～2005年的人民币汇率决定研究

本文基于1994~2005年人民币外汇市场制度特征,构建了一个央行频繁干预情形下的人民币汇率决定的市场微观结构模型。该模型发现:即使宏观基本面没有出现变化,汇率也依然会发生波动,指令流的变动是短期汇率波动的根本推动力。指令流通过交易商层面的短暂性资产组合效应和市场层面的持久性资产组合效应对汇率波动产生影响。我国中央银行为了维护日内涨跌幅限制下外汇市场有充分的流动性,必须被动地入市干预,中央银行的干预量由汇率决定模型内生决定。本文为人民币外汇市场参与者和监管当局初步打开了人民币汇率形成的“黑匣子“。北京大学汇丰金融研究院2009年课题;国家社科基金青年项目(09CJY083);国家社科基金重点课题(08ATL007);教育部人文社科基金青年项目(08JC790038);教育部人文社科重点研究基地重大项目(05JJD790075);国家自然科学基金项目(70673116);广东省哲学社会科学“十一五”规划青年项目(08YE-02);中山大学“985工程”产业与区域发展研究创新基地;广东省普通高校人文社会科学重点研究基地经费资助成果之

三门峡铝矿巷道支护机理分析及工程实践

根据三门峡铝矿岩层特点及工程概况,研究复杂地质条件下巷道围岩特性,结合现场勘察试验情况,得出围岩的破坏成因,并阐述围岩支护结构的作用机理,借鉴国内外相关的支护经验,给出有针对性的支护方案,详细阐明支护方案的施工要点及其工艺过程,从而达到全面合理的评价效果

D-loop Sequence Variation of Mitochondrial DNA in Captive Chinese Alligator

扬子鳄 (Alligatorsinensis)是中国特有的珍稀爬行动物 ,至 2 0 0 0年 ,野生扬子鳄的个体数已不足 15 0条 ,作为保护这一物种的措施之一 ,先后于 80年代初建起了 2个养殖场 ,现人工繁殖的扬子鳄总数已达 90 0 0余条。为揭示扬子鳄种群遗传多样性 ,从两个饲养种群中采集了 42个个体的样品 ,其中宣州样品 33个 (XZSP) ,长兴样品 9个(CXSP) ,用PCR方法扩增mtDNA控制区 ,扩增产物纯化后直接用ABI 310全自动遗传分析仪荧光标记测序 ,得到其中 39个个体的mtDNA控制区 5′端 46 2bp的序列。经比对发现 ,39个个体间的 5′端mtDNA控制区没有任何变异位点 ,共享一种单元型 ,提示扬子鳄饲养种群的遗传多样性非常贫乏 ,造成这一结果的主要原因是近 5 0年来 ,扬子鳄种群衰退和数量迅速减少导致的遗传多样性丢失 ,其次是人工繁殖的群体同时受到始创者数量较少产生的瓶颈效应影响。针对扬子鳄遗传多样性的现状 ,作者最后就这一濒危动物遗传多样性的保护对策提出 3点建议。Chinese alligator,Alligator sinensis,is a critically endangered endemic species under legislative protection in China.Result of recent investigations revealed that number of the alligators was continuously declining in the past 50 years and less than 150 individuals were surviving in the wild until 2000 years.In order to prevent the extinguishing of this species,two breeding farms were set up in early 1980s at Xuanzhou county,Anhui Province and Changxing County, Zhejiang Province respectively.After twenty years of breeding efforts,the number of captive individuals has been brought up to more than 9 000 in total, forming two separate captive subpopulations,Xuanzhou subpopulation (XZSP) and Changxing subpopulation (CXSP).Because of lack of the information regarding genetic diversity of the captive populations, 42 captive individuals including 33 individuals from XZSP and 9 from CXSP were sampled randomly to investigate their genetic status for the strategy in the next protection action.PCR method was adopted for amplification of mitochodrial DNA control region using primers designed in this research.After purification of PCR products,all of amplicons were sequenced directly with ABI BigDye TM Terminator Cycle Sequencing Ready Reaction Kit and ABI 310 genetic analyzer.Consequently, 5′end of control region with length of 462 base pair was obtained from 39 samples.Sequence alignment shows there is no any variation site in this range of control region among the individuals assayed here, namely only one haplotype of the region shared by these alligators.This result strongly indicates that the population of captive Chinese alligator is in very poor genetic diversity status.Reasons for the losing of genetic diversity in the population are mainly attributed to population depression and number of individual decreasing sharply in the past 50 years.Another factor accounting for the phenomena is the limit of founder number of captive population.Finally,authors proposed three pieces of advice for the genetic conservation of Chinese alligator.教育部骨干教师资助计划项目 (GG -180 - 2 10 0 2 40 3 -1740 );; 江苏省“333工程”人才培养基金资助~

人民币混合交易系统下的会员报价与交易策略

本文构建了人民币微观市场结构模型,在模型中引入做市商制度和询价交易方式,具体考察会员在不同市场、不同交易方式下的最优买-卖报价、价差、中间价以及在批发市场上的交易方式选择问题,为中国目前实行的混合交易系统下的会员报价和交易行为提供理论解释和模型分析。国家社科基金课题(06CJY002、07BJY167);国家自然科学基金项目(70473106、70673116);教育部人文社会科学重点研究基地重大项目(05JJD790075);福建省高等学校新世纪优秀人才支持计划资助成果之

TGF-β1 induces activation of HSC-T6 cells and epithelial-mesenchymal transition in rats

目的探讨转化生长因子-β1（TGF-β1）在大鼠肝星状细胞系（HSC-T6）活化及上皮间质转换（EMT）中的作用。方法体外培养HSC-T6，用MTT法筛选TGF-β1对HSC-T6作用的最佳浓度；用10μg／L TGF-β1处理HSC-T624h，相差倒置显微镜下观察细胞形态改变，免疫荧光染色法检测细胞骨架结构F-actin蛋白的表达，RT-qPCR法检测肌动蛋白α-SMA及代表上皮间质转换的神经黏附素（N-cadherin）、波形蛋白（vimentin）和上皮黏附素（E-cadherin）基因表达；用不同浓度（0、5和10μg/L）的TGF-β1处理HSC-T624h，Western blot检测α-SMA、N-cadherin、vimentin和E-cadherin蛋白表达。结果10μg/L TGF-β1干预HSC-T624h有最好的细胞存活率；TGF-β1刺激HSC-T6后，细胞拉伸，伪足增多呈星形，细胞间连接疏松，呈显著活化状态；F-actin聚集形成应力纤维丝，沿细胞长轴分布；实验组α-SMA mRNA及vimentin mRNA的表达量明显高于对照组（P〈0．05），而E-cadherin mRNA的表达量明显降低（P〈0．05）；在不同浓度的TGF-β1呈剂量依赖性致α-SMA及N-cadherin和vimentin的蛋白表达量增多，而E-cadherin的蛋白表达量减少。结论TGF-β1可诱导HSC-T6活化及上皮间质转换。Objective To observe the effect of TGF-β1 on activation and epithelial mesenchymal transition （EMT） in rat hepatic stellate cell-T6. Methods Adopt the MTT method to screen the optimum concentration of TGF-β1 in vitro HSC-T6 cultured. After the HSC-T6 stimulation by TGF-β1 of 10 μg/L for 24 hours, the morphology of the cells was observed under inverted phase contrast microscope, the expression of F-actin which on behalf of cotoskeletal structure was detected by immunofluorescence staining; the expression of α-SMA and N-cadherin, vimentin, E-eadherin was measured by RT-qPCR ; The changes of α-SMA, N-cadherin, vimentin and E-eadherin were assessed by Western blot after different concentrations （0,5 and 10 μg/L） of TGF-β1 interventing HSC-T6 for 24 h. Results The optimal cell survival rate was recorded when 10 μg/L TGF-β1 dealt with cells for 24 h. After HSC-T6 were treated with TGF-β1 ,cells stretched, pseudopodia increased and turn into stellate, ceils connections were looser, so that represented a significantly activated state. F-actin filaments gathered to form stress and distributed along the long axis of the cells ; The expression of α-SMA mRNA and vimentin mRNA in experimental group was significantly higher while E-cadherin mRNA was obviously lower than the control group （P〈 0. 05）. TGF-β1 made the protein expression of α-SMA and N-cadherin, vimentin in dose-dependent increased while E-cadherin was decreased.Conclusions TGF-β1 may induce activation and epithelial-mesenchymal transition of HSC-T6.福建省科技计划（2015Y01010224）;福州市科技计划（2014-S-137-1