Research on Cultivation of Students' Employability in Universities of Applied Sciences

大学生就业难是我国当代社会、经济和教育转型过程中普遍存在的问题，也是当前我国亟待解决的重大理论和实践问题之一。在结构性失业、选择性失业、岗位挤占、教育过度等诸多就业难问题中，大学毕业生因自身就业能力不足而产生的就业难问题凸显，尤其是毕业生的基本工作能力普遍低于工作岗位实际需要的问题十分突出，引发社会各界广泛关注。应用技术大学是我国高等教育大众化时期多样化发展的必然选择，也是地方本科高校可持续发展的现实选择。探讨转型时期我国应用技术大学学生就业能力的结构及培养问题，理论上有助于丰富我国有关大学生就业能力理论的本土化研究，实践上有利于提升大学生的就业质量和高校人才培养水平，政策上也有助于解决社会重...The difficulty of graduates’ employment is the common problem during the transition of China’s society, economy and education. Meanwhile, it is one of the significant theoretical and practical issues which has drawn great attention national wide. Among the problems of structural unemployment, optional unemployment, occupational squeeze and over-education, the unemployment problem caused by graduat...学位：教育博士院系专业：教育研究院_教育（博士、硕士）学号：2572012015358

基于“学工、教学深度融合”的学风建设途径研究

从“学工、教学深度融合“的思路出发,结合新建本科院校英语专业学生实际,提出适合新建本科院校学风建设的“主旋律““主阵地““主渠道““主力军““主抓手“和“主推力“的“六主“实现途径

论现代大学制度的自由精神

自由精神是现代大学的基本制度精神之一,而学术自由精神受自由学术理念支配,是现代大学制度自由精神的内核;独立精神是现代大学制度自由精神的前提,现代大学制度以制度形式确立了现代大学的独立自主和学术自由,缺乏独立的大学不可能真正享有自由,因而也不能冠以现代大学之名;大学的批判精神是推动大学追求卓越、实现超越的助推器,是大学自由精神的超越。全国教育科学“十二五”规划2013年度国家一般课题“现代大学制度研究——历史与现实的反思”(批号:BIA130082)阶段性成

跨海集群工程节能减排关键技术研究

港珠澳大桥跨海集群工程囊括跨海桥梁、沉管隧道、离岸人工岛等多个领域。跨海集群工程由于建设期及运营期能耗巨大,国内外尚无节能减排的成套技术指导工程建设,成为制约跨海集群工程持续发展的瓶颈之一。本文依托港珠澳大桥跨海集群工程建设和运营过程,采用理论研究、数值分析及模拟测试等多种方法,开展了集群工程节能减排指标体系和长大沉管隧道通风、照明、人工岛运营节能减排关键技术研究,成果支撑了工程项目节能减排目标的实现

教育部直属高校毕业生就业质量评价体系探析——基于教育部直属高校2013年就业质量年度报告的数据

对高水平大学而言,考察毕业生的就业状况不能仅限于就业率的高低,还必须将就业质量纳入评价体系,以符合国家、社会和高校自身的心理预期。据此,建构科学合理的就业质量评价体系就显得十分必要。文章以教育部直属高校2013年发布的毕业生就业质量年度报告为研究对象,在分析其就业质量评价体系内涵、结构和特征的基础上得出以下结论:总体上教育部直属高校的就业质量评价体系仍存在评价标准不同、评价主体单一、数据来源各异、统计口径不一、评价机制不够完善等诸多问题。这些问题在很大程度上影响到就业质量评价的科学性与权威性。要改变这一状况,一是要建立科学合理的评价指标体系,二是要明确就业质量评价数据的具体来源,三是要完善以第三方为评价主体的就业质量评价机制,四是要实现评价主体的多元和评价口径的一致。教育部人文社科重点研究基地重大项目“大学生结构性失业的教育审视与治理”(11JJD880026

Bacterial Conversion of Sulfur-and Phosphorous-Compounds and Microbial Diversity in Sediments from a Near-Shore Marine-Cultural Region

对福建某近海养虾场底泥环境中硫和磷 2种元素的微生物代谢进行了研究 .结果表明 ,细菌代谢有机硫和无机硫产H2 S是养殖过程中造成H2 S污染的主要因素 ,利用半胱氨酸和硫代硫酸钠产生硫化氢的细菌数量分别为 1 .6× 10. 6和 4 . 35× 10. 3个·g-1底泥 ;进一步研究发现 ,芽孢杆菌属、盐芽孢杆菌属和微杆菌属等细菌是产H2 S的优势菌群 ,而硫酸盐还原菌的数量较少 ,仅为 2 5个·g-1,其产H2 S的作用不明显 .研究还发现 ,转化有机磷和无机磷酸盐的优势菌群属于好氧细菌 ,其中分解卵磷脂的细菌和产磷酸酯酶细菌的数量分别为 2 . 17× 10. 5和 1 2. 1× 10 6个·g-1,转化磷酸钙的细菌数量为 6 . 96× 10 3 个·g-1.本文从微生物学的角度探讨了养殖环境中硫、磷化合物的转化 ,提出细菌好氧代谢产H2 S是养殖环境潜在的污染因素 ,给出了一些改善和修复养殖环境生态的建议 .The H_2S-producing bacteria and the phosphorous-cycling bacteria in sediments from near-shore marine-cultural region were investigated. Results indicate that the bacterial H_2S production in aerobic condition is the dominating process to produce H_2S in the sediment of cultural pond. The total counts of H_2S-producing bacteria utilizing cysteine and Na_2S_2O_3 were 1.6×106 and 4.35×103 cells g -1 respectively. The counts of sulfate-reducing bacteria in the sediments were very little, only 2.5×101 g -1. Further results show that the bacterial counts of decomposing lecithin and secreting phosphatase were 2.17×105 and 1.21×106 g -1 respectively, bacterial counts of dissolving Ca_3PO_4 were 6.96×103 g -1. Traditional taxonomy and partial 16S rRNA gene sequencing on the H_2S-producing and phosphate-cycling bacteria indicate that most isolates could be classified as members of the following Genera: Bacillus, Halobacillus, Microbacterium, etc.中国科学院知识创新工程项目 (KZCX1 SW 12 Ⅱ ) ;; 中国科学院百人计划项目

Expression and activity characterization of thrombosis targeting protein hu3D3V_H-tTF to blood vessel of lung cancer

目的制备一种用于肺癌血管靶向栓塞治疗的融合蛋白hu3D3VH-tTF,并鉴定其生物学活性。方法利用重叠PCR技术构建tTF与hu3D3VH的融合基因,克隆至表达载体pET22 b(+),在E.coliBL21(DE3)中表达,镍亲和色谱柱纯化目的蛋白。ELISA检测融合蛋白hu3D3VH组分与肺腺癌细胞A549选择性结合活性,凝血实验和FⅩ活化实验鉴定融合蛋白tTF组分的促凝血活性。结果获得序列正确的hu3D3VH/tTF/pET22 b(+)重组子,融合蛋白在E.coliBL21(DE3)中高效表达。纯化后的融合蛋白与肺腺癌细胞A549具有选择性结合活性,并能活化FⅩ、有效促发血液凝固。结论成功构建hu3D3VH/tTF/pET22 b(+)重组子,hu3D3VH/tTF融合蛋白具有hu3D3VH的选择性结合能力同时具有TF的促凝血活性,为开展选择性肺肿瘤血管血栓性栓塞研究奠定了基础。 【英文摘要】 Purpose To prepare the fusion protein of hu3D3VH-tTF for thrombosis targeting therapy of lung cancer and to analyze its biological activities.Methods Fusion gene hu3D3VH-tTF was constructed by overlap PCR,cloned into expression vector pET22 b(+),and expressed in E.coli BL21(DE3).The fusion protein hu3D3VH-tTF was purified through Nickel-affinity chromatography column.The selective binding activity of the hu3D3VH moiety of fusion protein was analyzed using ELISA and the coagulation activity of the tTF moiety...福建省自然科学基金项目(C0410004);; 厦门大学科技创新项目(XDKJCX20053026

ECOLOGICAL CHARACTERIZATION OF BACILLI IN SEDIMENT FROM A NEAR-SHORE MARINE HORTICULTRTAL REGION

通过对近海养虾场底泥中的细菌数量和类群的调查 ,发现有超过 5 0 %的细菌生物量是产芽孢细菌 ,因此对底泥中的产芽孢细菌进行了分离和纯化 ,通过对细胞形态、生理生化等特征的研究和对部分菌株的 16SrRNA基因的ARDRA分型、序列分析等 ,鉴定了 6 7株产芽孢细菌 ,其中 6 2株属于芽孢杆菌属 ,5株属于短芽孢杆菌属 .进一步对 6 2株芽孢杆菌属的细菌在底泥不同深度的分布进行研究 ,结果表明 ,巨大芽孢杆菌主要分布在底泥深度 0～ 6cm左右的区域 ,海洋芽孢杆菌、短小芽孢杆菌和蜡状芽孢杆菌主要分布于底泥 6cm以下的区域 ,与坚强芽孢杆菌性状相近的菌分布在底泥 2～ 8cm深度 ;与耐碱芽孢杆菌性状相近的芽孢菌广泛分布在 0～ 12cm区域 .讨论认为 ,应用这些产芽孢细菌资源在修复海洋环境和开发海水养殖微生态制剂方面具有一定可能性 .图 3表 3参 15A survey on microbial populations in the sediment from a near-shore mariculture region revealed that over 50% (by cells) of the biomass was spore-producing bacteria. Totally 67 strains of spore-producing bacteria were obtained and were identified by their cellular morphology, physiological and biochemical features. 12 of the 67 strains were further characterized by cloning their 16S rRNA genes. Results indicate that 62 out of the 67 spore-producing bacterial strains belong to the genus Bacillus and 5 strains belong to Brevibacillus. Among the 62 strains of Bacillus, B. megaterium, B. marinus, B. pumilus, B. cereus, B. thuringensis, B. sphaericus and B. firmus were identified, while other 28 strains could not be taxonomically identified and need further studies. The strains of B. megaterium mainly distributed in the zone above 6 cm of the sediments, and B. pumilus and B. cereus were dominant species in the sediment under depth of 6 cm. The unidentified bacilli distributed through out the entire zone (0～12 cm) of the sediment. The possibility of applying these bacilli for bioremediation of the polluted marine environments or for developing ecological preparations for horticultural is discussed. Fig 3, Tab 3, Ref 15中国科学院知识创新工程项目 (KZCX1 SW 1 2 Ⅱ );; 百人计划支持项目~

Analysis of Nitrate Reducing Community in a Near-shore Marine-cultural Sediments

通过对福建省沿海海水养殖场沉积物中参与氮循环的各生理群细菌数量分析 ,发现氨化和硝酸盐还原细菌是优势生理菌群 ,同时 ,表层泥样中的硝酸盐还原菌数量明显高于深层泥样。从该环境中分离获得 1 0 6株细菌 ,其中 5 8株具有硝酸盐还原能力 ,初步鉴定表明它们主要为芽孢杆菌属 (Bacillus)、盐芽孢杆菌属 (Halobacillus)、短芽孢杆菌属 (Brevibacil lus)、动性球菌属 (Planococcus)和动性杆菌属 (Planomicrobium)等革兰氏阳性细菌的成员 ;1 6SrRNA基因序列分析进一步证实该环境中的硝酸盐还原菌具有丰富的多样性The nitrogen-cycling bacteria in a near-shore marine-cultural sediments were investigated. Results indicated that ammonifying and nitrate-reducing bacteria (NRB) were the most abounding populations. The abundance of nitrate-reducing bacteria occurred in the surface layer of the sediment was higher than that in the bottom layer. 106 bacterial strains were obtained from sediment samples and were tested for their nitrate reducing ability. The results showed that 58 strains of them were able to reduce nitrate. The dominant nitrate-reducing strains were preliminarily identified as Gram positive bacteria and belong to the genus of Bacillus, Halobacillus, Brevibacillus, Planococcus and Planomicrobium.The richness of diversity of nitrate-reducing bacteria was further revealed by the analysis of the sequences of their 16S rRNA genes

渤海典型生态灾害的发展变化特征及演变趋势

渤海是中国的内海,受海岸带地区人为活动的影响,海域生态环境退化严重,其主要表现为海洋生态灾害频发。本文对渤海近年典型生态灾害,如赤潮、大型藻类及水母发生的时空分布特征进行了总结,结合水环境变化,对其演变趋势进行了评估,并提出了相应的防控应对建议