138 research outputs found

    The influence of FDI on China's Upgrading of Industrial Structure

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    经济发展必然要求实现产业结构的升级。在我国经济发展面临资源、人口、环境以及过分依赖外贸等诸多压力的情况下,产业结构升级的速度应当进一步加快。我国的产业结构升级是在经济开放、世界经济日益一体化的条件下实现的。外商直接投资,作为一种一揽子资源,除给我国的经济发展带来必要的建设资金外,还带来管理、技术等发展经济不可或缺的软资源。因此,外商直接投资作为我国市场经济中的一种强势力量,对我国的经济运行必然产生重要的影响,产业结构升级也不例外。 据此,本文主要从以下几个方面来探讨外商直接投资对我国产业结构升级的影响。 第一章在阐释了本文选题的背景与意义后,对国内外关于外商直接投资研究的情况作了一个简要的...It is a natural process for an economy to fulfill its industrial structure upgrading. But as to China, it seems to be pressing to accelerate this process, because China is now facing too many kinds of constrains, including resources, a great amount of people, environment and the more dependent to foreign market. China’s upgrading of industrial structure is under the background of global economy in...学位:经济学硕士院系专业:经济学院经济系_政治经济学学号:20040900

    Analysis of Jump Behavior in China's Stock Market

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    股票价格的具体波动特征在资产定价、风险管理以及投资组合等金融理论中都是一个极为重要的考虑因素。区别于收益率的可观测性,波动率%具有不可观测的性质,如何准确有效的估计波动率成为学界和业界共同关注的问题。在大部分时间内,股票价格的波动较为平稳,体现出连续性的特征,但在某些时点会出现大幅度的突变行为,即跳跃,体现出非连续性的特征。本文利用多种方法考察我国股票市场的波动特征后,利用具有无限变差的时变Lévy过程建立统一的模型刻画市场总体波动中的各种成分。所建立的模型考虑到了收益率分布的尖峰厚尾、波动聚集、杠杆效应以及多种跳跃等诸多特征,并运用贝叶斯方法对模型进行了估计。 本文首先进行了一个简要的文献...Volatility is one key important factor in financial theories, especially for asset pricing, risk management and portfolio. Since volatility is unobservable, how to measure it more accurately is concerned by scholars and practitioners. Volatility is stable most of the time, but has big jumps sometimes. In this paper, I want to discuss how to construct models which can capture all different types of...学位:经济学博士院系专业:王亚南经济研究院_西方经济学学号:2772007015389

    从五大产茶国的比较中看我国茶业存在的问题和对策

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    在世界58个产茶国和30多个茶叶出口国中,中国、印度、斯里兰卡、肯尼亚和印度尼西亚五国的产量和出口量约占世界总量的80%以上,所以这五个国家茶业的发展情况决定着世界茶业的兴衰,而世界茶叶市场的竞争也主要在这五个国家之间展开。为了分析我国茶业存在的问题,文章在生产状况、产品结构、贸易状况三个方面对五国进行比较分析,已提出适合我国茶业发展的对策

    The effect of nurse initiated patient/family education strategy on people with schizophrenia in Beijing

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    2004-2005 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

    Construction of eukaryotic vector pcDNA3.1-flag-pygo2 and expression in C6 cell

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    目的构建pcDNA3.1-flag-pygo2真核表达载体并在C6细胞中进行表达。方法从小鼠脑胶质细胞中提取总RNA,RT-PCR法反转录合成c DNA,设计引物,调取目的片段,与pcDNA3.1-flag载体连接后转化大肠杆菌DH5α,LB平板筛选菌落,提取质粒。重组质粒pcDNA 3.1-flag-pygo2经过酶切鉴定及测序后,阳离子脂质体法转染C6细胞并经免疫细胞化学染色及蛋白印迹检测重组体的表达。结果重构质粒pcDNA 3.1-flag-pygo2经限制性核酸内切酶EcoRⅠ,HindⅢ酶切分析及测序检查,表明真核表达载体构建正确;瞬时转染C6细胞后,免疫细胞荧光染色及蛋白印迹检测表明转染细胞能够表达外源Pygo2基因。结论成功构建了pcDNA3.1-flag-pygo2真核表达载体并能够在真核细胞中进行表达,这为今后研究pygo2基因在胶质瘤中的作用机制奠定了基础。Objective To construct the eukaryotic expression vector pcDNA3.1-flag-pygo2 and express the combined protein in C6 cell line.Methods To extract total RNA from primary glial cell of mouse and to synthesize c DNA by RT-PCR,then design primer and clone whole segment of pygo2 gene.After the targeted gene was inserted into vector pcDNA3.1-flag,the recombined plamid was transformed into E coli DH5α for LB agar plate screening and the recombined plasmid were extracted and purified.After verification by double enzyme digestion and sequencing.,the constructed eukaryotic expression plamid was transfected to C6 cell line by lipofectamin method,finally the protein expression was detected by immunocytochemical staining as well as western blot.Results The new constructed vector was confirmed by restricted enzyme Eco R I,HindIII digestion assay and correct Pygo2 was verified by sequenceing.finally,pcDNA3.1-flag-hpygo2 can express exogenous pygo2 gene in glioma C6 cell line after transient transfection by the determination of immunocytofluorescent staining and western blot.Conclusion The new plamid pcDNA3.1-flag-pygo2 was constructed successfully,and can express fused protein in eukaryotic cell,which establish the foundation for future research on pygo2 gene function in human glioma

    人民币套息交易:市场基础和收益风险特征

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    在全球经济金融虚拟化背景下,利率与汇率的波动性增强。以跨国公司为代表的经济主体为实现利润最大化目标,在全球进行资源优化配置,频繁进出利率与汇率市场,进行套息交易。相应交易的长期性和广泛性表明,利率平价理论提出的利率与汇率间的平价均衡关系并不成立,套息交易对金融系统安全性具有负面影响。在资本账户未完全放开的条件下,关于人民币套息交易机理机制的实证研究表明,人民币套息交易有多种途径,大宗商品交易是较突出的渠道。因此,在人民币国际化过程中须健全金融监管,协调好资本账户开放的步骤与速度,防范套息交易危害实体经济发展。国家自然科学基金项目“资金流、交易者异质性与股市波动”(71373225)阶段性成

    Over-expression of Pygo2 Promotes C6 Cells Proliferation of Glioma

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    目的通过构建过表达PygO2的重组体上调PygO2表达,探讨其在大鼠胶质瘤C6细胞增殖中的作用及机制。方法重组体经ECOr I和HIndⅢ双酶切鉴定和dnA测序后,用脂质体2000将其转染大鼠胶质瘤C6细胞,采用WESTErn blOT检测外源PygO2蛋白表达,应用克隆形成实验和MTT法检测细胞增殖,流式细胞术检测细胞周期,采用WESTErn blOT检测过表达PygO2对C6细胞中CyClInd1、β-CATEnIn水平的影响,并采用细胞免疫荧光法检测其对C6细胞中CyClInd1、β-CATEnIn亚细胞定位的影响。结果双酶切和测序鉴定结果证实插入序列正确,重组体能有效上调PygO2表达。将重组体转染C6细胞上调PygO2表达后,细胞的生长增殖被显著促进,克隆形成显著增多,细胞周期进程从g1期至S期转变显著增强;且CyClInd1水平随之增高,亚定位无改变,β-CATEnIn水平和亚细胞定位无明显改变。结论成功构建了过表达PygO2的重组体,过表达PygO2通过增高CyClInd1水平,促进细胞从g1期进入S期,从而促进大鼠胶质瘤C6细胞增殖。Objective To up-regulate expression of Pygopus2(Pygo2) by construction of the recombinant vectors of over-expression of Pygo2 protein,and to explore the role and mechanism of over-expression of Pygo2 in C6 cells proliferation of glioma.Methods The recombinant plasmids were digested with EcoRⅠ and Hind Ⅲ to execute the restriction endonuclease identification,then the sequence analysis was assayed by DNA sequencing.The recombinant plasmids were transfected into cultured glioblastoma C6 cells using lipofectamineTM 2000.The exogenous Pygo2 protein level of C6 cells was detected by Western blot analysis.Colony forming assay and MTT assay were used to detect the cell proliferation,and cell cycle analysis was performed by flow cytometry analysis.The effect of Pygo2 over-expression on the level of cyclinD1 and β-catenin of C6 cells was detected by Western blot analysis,and the expression and subcellular location of cyclinD1 and β-catenin of C6 cells were further quantified by immunofluorescent staining.Results The recombinant plasmids were completely coincided with the designs by the restriction map and the sequence analysis,which up-regulated Pygo2 expression of C6 cells efficiently.After Pygo2 expression were up-regulated by transfected C6 cells with the recombinant plasmids,cells proliferation was promoted and colony forming was increased significantly,cell cycle progression from G1 to S transition was enhanced notably.Furthermore,the expression level of cyclinD1 was significantly increased without change of subcellular location,and the expression level and subcellular location of β-catenin were not changed obviously.Conclusion The recombinant vectors of Pygo2 over-expression were constructed successfully.Over-expression of Pygo2 promotes the growth of glioma cells by an increased expression of cyclinD1 to improve G1/S transition.重庆市自然科学基金资助项目(cstc2011jjA10110);重庆市教委科技基金资助项目(KJ100504);福建省自然科学基金资助项目(2009D002

    青海高原牛羊双腔吸虫病病原生物学的初步调查

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    不文报道在青海高原对牛羊双腔吸虫病病原生物学初步调查的结果。从29个流行地区绵羊收集的标本检查结果,发现有四种双腔吸虫,它们是中华双腔吸虫(Dicrocoelium chinensis)、矛形双腔吸虫(D.lanceatum)、客双腔吸虫(D.hospes)和枝双腔吸虫(D.dendriticum)。从三个流行区检查Helicella candacharica及Cathaica przewalskii二种陆地蜗牛,查到四种双腔吸虫成熟尾蚴,它们是中华双腔吸虫、矛形双腔吸虫及二种未定名的双腔吸虫尾蚴,此二种尾蚴是何种双腔吸虫尾蚴,尚待进一步研究。检查流行区中7种蚂蚁是于9月份从乐都的Formica gagates查到双腔吸虫囊蚴,其形态与中华双腔吸虫囊蚴十分相像

    Mental health of elderly victims in relocation sites four months after the 5·12 earthquake

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    目的评估5.12地震后4个月安县板房区老年人的心理健康状况,并探讨其影响因素。方法利用自编受灾情况调查问卷、中国心理健康量表(老年版)(CMHI-E)、幸福感量表(MUNSH)和流调中心用抑郁量表(CES-D),调查绵阳市两个安置板房区290位老年人。结果灾区老年人的心理健康水平和幸福感水平均低于对照样本(P<0.001);抑郁水平高于对照样本(P<0.001);心理健康得分和幸福感正相关(r=0.61,P<0.01);心理健康得分和抑郁得分负相关(r=-0.59,P<0.01)。多元回归分析显示影响灾区老年人心理健康的主要因素有亲属遇难人数、财产损失状况和年龄。结论重灾区老年人的心理健康状况较差,幸福感水平较低,抑郁程度较高。遇难的亲属越多、年龄越大、财产损失越严重,老人的心理健康状况越差

    浅谈环境管理中的公众参与

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    &lt;p&gt;公众参与是人民管理国家事务和社会事务权、公众环境监督权、环境知情权、环境立法参与权、环境影响评价参与权、环境决策参与权和环境诉权为公众参与环境保护提供了丰厚的权利基础,为了保障和推动公众参与环境保护活动的发展,我国立法应明确公众参与环境影响评价的效力,更加重视公众环境决策参与权的实现,构建符合中国国情的环境公益诉讼制度。&lt;/p&gt;</jats:p
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