山水校园,寓教于景——以福建省大田县职业中专新校区设计项目为例

文章从大田县职业中专新校区的设计出发,塑造出大田县职业中专新校区独特的校园风貌,研究了基于山水校园理念的整体校园设计方法,总结出山地校园的规划和建筑设计应该从周边环境出发,充分考虑基地特征、城市需求和周边自然环境,并突破传统校园规划模式,为山地新校区结合自然环境山水规划设计提供有价值的设计思路和经验

Oil Spill identification by near-infrared spectroscopy coupled with clustering analysis based on principle components

鉴于目前没有一种方法能独立解决溢油鉴别的所有问题,本文提出了近红外光谱技术结合主成分聚类分析鉴别溢油种类的方法。通过有机溶剂萃取出自行配制的汽油、柴油和润滑油模拟样品中的溢油后记录其近红外光谱,对5800～6200cm-1区段范围内的谱图经多元散射校正(MSC)、Norris一阶导数平滑预处理处理后求其主成分,并在主成分的基础上引入Ward聚类分析法(离差平方和法)对样品分类。结果表明近红外光谱技术结合聚类分析能对体积分数在0.4～0.8mL/L间的海面溢油样品正确、快速分类,近红外光谱技术结合主成分聚类可作为溢油鉴别的一种辅助方法。Owing to none of the existing method can do it all for oil identification, but a method by near-infrared spectroscopy (NIR) coupled with clustering based on principle components is proposed. Petroleum oil stimulating samples of gasoline, diesel fuel and lubricating oil were employed to develop the method. NIR spectra ranges of 5800～6200cm~(-1) were recorded with the multiplicative signal correction and Norris first derivative filter pretreatments. The clustering analysis by Ward's method base on the different principle components was discussed. The results show that the method provides the rapid and accurate classification for spilled oil with concentration between 0.4～0.8 μL/L.国家高技术发展计划(863)资助项目(2001AA635040

用户社群与企业互动创新中知识转移效果提升途径

互动创新中知识在用户社群和企业之间的转移是一项复杂的、系统的工程,受到多主体、多因素的影响。针对如何充分发挥正面影响因素对知识转移的促进作用和减轻反面影响因素的阻碍作用的问题,文章从互动机制、知识运作机制和学习机制三方面分析了用户社群与企业互动创新中知识转移效果的提升路径。国家软科学研究计划项目“海峡西岸经济区企业知识治理机制及应用研究”(项目号:2010GXQ5B293); 福建省科技计划项目(软科学项目)“福建省战略性新兴产业的发展现状评价及对策研究”(项目号:2013R0069

基于共生思想的山地住区规划设计——以清流县“怡景书苑”规划及建筑设计为例

随着人们环境意识的增强,对住区景观环境建设的要求也越来越高,如何利用原生山体景观使住区与场地更加契合成为建筑设计的重点。以清流县"怡景书苑"住区规划及建筑设计为例,结合黑川纪章的共生思想,从总体规划、建筑设计、景观营造等方面入手,探讨顺应原生环境特征的设计手法,为闽中山地住区设计提供新的思路

Over-expression of Pygo2 Promotes C6 Cells Proliferation of Glioma

目的通过构建过表达PygO2的重组体上调PygO2表达,探讨其在大鼠胶质瘤C6细胞增殖中的作用及机制。方法重组体经ECOr I和HIndⅢ双酶切鉴定和dnA测序后,用脂质体2000将其转染大鼠胶质瘤C6细胞,采用WESTErn blOT检测外源PygO2蛋白表达,应用克隆形成实验和MTT法检测细胞增殖,流式细胞术检测细胞周期,采用WESTErn blOT检测过表达PygO2对C6细胞中CyClInd1、β-CATEnIn水平的影响,并采用细胞免疫荧光法检测其对C6细胞中CyClInd1、β-CATEnIn亚细胞定位的影响。结果双酶切和测序鉴定结果证实插入序列正确,重组体能有效上调PygO2表达。将重组体转染C6细胞上调PygO2表达后,细胞的生长增殖被显著促进,克隆形成显著增多,细胞周期进程从g1期至S期转变显著增强;且CyClInd1水平随之增高,亚定位无改变,β-CATEnIn水平和亚细胞定位无明显改变。结论成功构建了过表达PygO2的重组体,过表达PygO2通过增高CyClInd1水平,促进细胞从g1期进入S期,从而促进大鼠胶质瘤C6细胞增殖。Objective To up-regulate expression of Pygopus2(Pygo2) by construction of the recombinant vectors of over-expression of Pygo2 protein,and to explore the role and mechanism of over-expression of Pygo2 in C6 cells proliferation of glioma.Methods The recombinant plasmids were digested with EcoRⅠ and Hind Ⅲ to execute the restriction endonuclease identification,then the sequence analysis was assayed by DNA sequencing.The recombinant plasmids were transfected into cultured glioblastoma C6 cells using lipofectamineTM 2000.The exogenous Pygo2 protein level of C6 cells was detected by Western blot analysis.Colony forming assay and MTT assay were used to detect the cell proliferation,and cell cycle analysis was performed by flow cytometry analysis.The effect of Pygo2 over-expression on the level of cyclinD1 and β-catenin of C6 cells was detected by Western blot analysis,and the expression and subcellular location of cyclinD1 and β-catenin of C6 cells were further quantified by immunofluorescent staining.Results The recombinant plasmids were completely coincided with the designs by the restriction map and the sequence analysis,which up-regulated Pygo2 expression of C6 cells efficiently.After Pygo2 expression were up-regulated by transfected C6 cells with the recombinant plasmids,cells proliferation was promoted and colony forming was increased significantly,cell cycle progression from G1 to S transition was enhanced notably.Furthermore,the expression level of cyclinD1 was significantly increased without change of subcellular location,and the expression level and subcellular location of β-catenin were not changed obviously.Conclusion The recombinant vectors of Pygo2 over-expression were constructed successfully.Over-expression of Pygo2 promotes the growth of glioma cells by an increased expression of cyclinD1 to improve G1/S transition.重庆市自然科学基金资助项目(cstc2011jjA10110);重庆市教委科技基金资助项目(KJ100504);福建省自然科学基金资助项目(2009D002

闽西客家小镇街巷重构与表层更新——以清流县滨河空间及核心街区更新项目为例

随着社会经济快速增长,乡镇建设持续发展,\"千城一面\"现象也日渐突显。现今乡镇街巷重构与建筑表层更新活动愈发受到重视,而富有地方特色的建筑表层与街巷空间对塑造乡镇形象和传承地方文脉有重大作用。文章以福建省三明市清流县滨河空间及核心街区更新项目为例,在街巷重构与建筑表层更新层面,研究材质、色彩等建筑符号手法运用对于闽西客家传统建筑文化的重塑,探讨具有地方传统特色的建筑立面的改造设计手法,为闽西客家更新与改造建筑设计提供参考

An Investigation of the Professional Development of Primary and Secondary School P. E. Backbone Teachers in Fujian Province

采用文献法、调查法、统计法、逻辑法等研究方法,以参加2013—2015年福建省基础教育万名骨干教师培训中的190名中小学体育骨干教师为调查对象,对教师基本情况和教师专业发展的认识、态度、知识、能力、继续教育等现状进行调查,在此基础上对教师专业发展现状进行分析,总结出我省中小学体育骨干教师专业发展的特点及存在的问题,并提出相应的建议,给我省中小学体育教师培训机构的改革提供依据,同时为我省中小学体育教师专业发展提供参考与借鉴,为进一步深化我省中小学体育教育改革提供人才保障.结果表明:1)目前,福建省中小学体育骨干教师以年富力强者居多,年龄、职称结构较合理;教师专业发展意识强,学历层次由大专逐步向本科、硕士过度,但硕士比例小;2)教师专业发展态度端正,职业的稳定性和责任感强烈,但部分年轻教师缺乏专业发展的自主性;3)教师对专业知识的掌握比较扎实,主要通过互联网、图书资料、专家报告或讲座等方法来获取专业知识;4)专业能力水平方面,教师在体育教学、课外指导、体育保健、现代教育技术运用等方面能力比较强,而创新教育和科研两方面能力相对比较薄弱;5)当下,我省中小学体育骨干教师最需要强化的培训是课题申报技能、论文写作能力、专题讲座能力、教育科研方法、提高学历层次、教学设计能力,其中提高科研能力和水平是教师们最迫切的愿望.2014年福建省中青年教师教育科研项目(社科A类)(JAS14023

The effect of nurse initiated patient/family education strategy on people with schizophrenia in Beijing

2004-2005 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Effect of Temperature on Sensing Performance of Optical Fiber Relative Humidity Sensor Based on Nafion-crystal Violet Film

The optical fiber relative humidity sensor based on Nafion-crystal violet film was developed. The effect of samples temperature in the range from 299.15 K to 324.15 K on the sensing performance of the sensor was investigated. The mathematical function between temperature and the sensitivity of the sensor was established according to the reactive theory of sensor to the relative humidity, which was validated with the experiment. With the research, the application range of the sensor was extended, which made the sensor keep a good veracity when it was used in situ.国家“八六三”计划海洋监测技术主题项目(批准号:818-09-07)资助

102例初诊2型糖尿病患者中医病理特点分析

现阶段糖尿病已经成为非传染性的流行病。由于检测手段的先进性和人们健康意识的普遍提高,很多2型糖尿病患者是由于“健康“体检所发现,其临床表现已非传统消渴之“三多一少“所能概括。故运用中医学的研究方法探讨初诊2型糖尿病患者的中医病理特点,就显得尤为必要。本研究对厦门地区初诊2型糖尿病患者进行规范而全面的临床资料收集,运用中医证素辨证的方法,探讨初诊2型糖尿病福建省卫生厅资助课题(WZZZ0903