TGF-β1-induces activation of HSC-T6 and epithelial-mesenchymal transition in rats

目的：研究转化生长因子-β1(TGF-β1)在大鼠肝星状细胞-T6细胞系(HSC-T6)活化及上皮间质转化(EMT)中的作用及其对TGF-β1/Smads信号通路的影响，揭示肝纤维化的发生与发展的分子病理机制，为肝纤维化的逆转治疗及肝硬化的防治提供新靶点。 方法：体外培养大鼠肝星状细胞-T6细胞系，用四甲基偶氮唑盐(MTT)法筛选TGF-β1对肝星状细胞-T6细胞系的最适作用浓度；用MTT法筛选出的最适作用浓度(10μg/L)的TGF-β1处理肝星状细胞24小时，倒置相差显微镜下观察细胞形态学的改变，免疫荧光染色法检测细胞内纤维形肌动蛋白(fibrousactin，F-actin)的表达，R...Objective: To observe the effect of TGF-β1 on activation and epithelial-mesenchymal transition, to investigate the effects of TGF-β1 on TGF-β1/Smads signaling pathway in rat hepatic stellate cells-T6,reveal the pathogenesis of hepatic fibrosis, and provide new target for the treatment of hepatic fibrosis and liver circosis. Methods: Adopt the MTT method to screening the optimum working concentra...学位：医学硕士院系专业：医学院_内科学学号：2452014115350

TGF-β1 induces activation of HSC-T6 cells and epithelial-mesenchymal transition in rats

目的探讨转化生长因子-β1（TGF-β1）在大鼠肝星状细胞系（HSC-T6）活化及上皮间质转换（EMT）中的作用。方法体外培养HSC-T6，用MTT法筛选TGF-β1对HSC-T6作用的最佳浓度；用10μg／L TGF-β1处理HSC-T624h，相差倒置显微镜下观察细胞形态改变，免疫荧光染色法检测细胞骨架结构F-actin蛋白的表达，RT-qPCR法检测肌动蛋白α-SMA及代表上皮间质转换的神经黏附素（N-cadherin）、波形蛋白（vimentin）和上皮黏附素（E-cadherin）基因表达；用不同浓度（0、5和10μg/L）的TGF-β1处理HSC-T624h，Western blot检测α-SMA、N-cadherin、vimentin和E-cadherin蛋白表达。结果10μg/L TGF-β1干预HSC-T624h有最好的细胞存活率；TGF-β1刺激HSC-T6后，细胞拉伸，伪足增多呈星形，细胞间连接疏松，呈显著活化状态；F-actin聚集形成应力纤维丝，沿细胞长轴分布；实验组α-SMA mRNA及vimentin mRNA的表达量明显高于对照组（P〈0．05），而E-cadherin mRNA的表达量明显降低（P〈0．05）；在不同浓度的TGF-β1呈剂量依赖性致α-SMA及N-cadherin和vimentin的蛋白表达量增多，而E-cadherin的蛋白表达量减少。结论TGF-β1可诱导HSC-T6活化及上皮间质转换。Objective To observe the effect of TGF-β1 on activation and epithelial mesenchymal transition （EMT） in rat hepatic stellate cell-T6. Methods Adopt the MTT method to screen the optimum concentration of TGF-β1 in vitro HSC-T6 cultured. After the HSC-T6 stimulation by TGF-β1 of 10 μg/L for 24 hours, the morphology of the cells was observed under inverted phase contrast microscope, the expression of F-actin which on behalf of cotoskeletal structure was detected by immunofluorescence staining; the expression of α-SMA and N-cadherin, vimentin, E-eadherin was measured by RT-qPCR ; The changes of α-SMA, N-cadherin, vimentin and E-eadherin were assessed by Western blot after different concentrations （0,5 and 10 μg/L） of TGF-β1 interventing HSC-T6 for 24 h. Results The optimal cell survival rate was recorded when 10 μg/L TGF-β1 dealt with cells for 24 h. After HSC-T6 were treated with TGF-β1 ,cells stretched, pseudopodia increased and turn into stellate, ceils connections were looser, so that represented a significantly activated state. F-actin filaments gathered to form stress and distributed along the long axis of the cells ; The expression of α-SMA mRNA and vimentin mRNA in experimental group was significantly higher while E-cadherin mRNA was obviously lower than the control group （P〈 0. 05）. TGF-β1 made the protein expression of α-SMA and N-cadherin, vimentin in dose-dependent increased while E-cadherin was decreased.Conclusions TGF-β1 may induce activation and epithelial-mesenchymal transition of HSC-T6.福建省科技计划（2015Y01010224）;福州市科技计划（2014-S-137-1

纳米La_2O_3催化剂上低温甲烷氧化偶联和乙烷氧化脱氢

采用沉淀法制备了纳米La2O3催化剂,并考察了该催化剂对甲烷氧化偶联和乙烷氧化脱氢反应的催化性能。实验结果表明,对于甲烷氧化偶联反应,在450℃、气态空速(GHSV)=7.5L/(g.h)、n(CH4)∶n(O2)=3.0的条件下,甲烷转化率和C2烃收率分别达到26.6%和10.8%,比商品化的La2O3催化剂的启动温度低100℃,具有较好的低温甲烷氧化偶联反应性能;对于乙烷氧化脱氢反应,在450℃、GHSV=10L/(g.h)、n(C2H6)∶n(O2)∶n(N2)=1∶1∶4的条件下,乙烷转化率和乙烯收率分别为49.1%和25.9%,明显优于商品化的La2O3催化剂。对纳米La2O3催化剂的表征结果显示,沉淀法制备的纳米La2O3催化剂颗粒较小(粒径30~50nm)、比表面积较大(12.0m2/g),具有较强的吸附O2能力,因此能在较低温度下活化甲烷和乙烷,具有较好的低温催化性能

BaF_2/La_2O_3催化剂上甲烷氧化偶联反应活性氧物种的原位红外光谱

用原位红外光谱研究了BaF2/La2O3催化剂上甲烷氧化偶联反应的活性氧物种.结果表明,催化剂经O2预吸附后,在1 108~1 118 cm-1处出现超氧物种O2-的O-O键伸缩振动峰.经18O2同位素交换实验后,原1 108~1 118 cm-1处谱峰的强度减弱,同时在1 086和1 051 cm-1处出现(O18O)-物种和18O2-物种的吸收峰.同位素交换实验进一步确证了1 108~1 118cm-1处谱峰确为O2-物种的吸收峰.在700℃下,O2-物种能够活化CH4生成C2H4,而且O2-物种的消耗量和C2H4的生成量呈很好的消长对应关系.超氧物种O2-是BaF2/La2O3催化剂上甲烷氧化偶联反应的活性氧物种

水解法提取纯化刺槐素的工艺研究

目的优选野菊花中刺槐苷的水解工艺。方法采用均匀设计法,以高效液相色谱法(HPLC)测定野菊花中刺槐素含量,探讨影响刺槐素提取效率的因素,并筛选出最佳提取条件。结果最佳水解工艺为盐酸与药液比为5∶2,盐酸浓度3 mol/L,水解时间为3 h,水解温度为100℃。结论 HPLC法测定野菊花中刺槐素含量的方法,快速、可行。均匀设计法优选的野菊花中刺槐素提取工艺,操作简单,节约时间,提取率比原工艺有明显提高

Assembling forms of active phases confined within SBA-15 channels

The assembling forms of active phases in Pd/SBA-15(ad) and MoO3/SBA-15 catalyst samples, prepared via adsorption-reduction and impregnation-reduction, respectively, were characterized by N-2 physisorption measurement. Based on N-2 adsorption-desorption isotherms, it was speculated that the palladium,species in Pd/SBA-15(ad) exist as nanoparticles, whereas the MoO3 species in MoO3/SBA-15 are layered dispersed on the wall within the SBA-15 channels. These speculations were further confirmed by HR-TEM observation. The SBA-15 confined uniform Pd nanoparticles were efficient for solvent-free aerobic oxidation of benzyl alcohol

Temporal and spatial distribution characteristics and driving factors of the Mid-Holocene settlements in the Weihai area

Background, aim, and scope Understanding the relationships among humans, climate, and ecosystem is one of the focuses of the PAGES. The Mid-Holocene was a significant period for germination and development of human civilization. During this time, the human tribe was thriving. Thus, it is valuable to study the temporal and spatial patterns of Mid-Holocene settlement sites because it can help refine strategies for future changes from past patterns. Therefore, it has become a hot topic in the study of the relationships between humans and earth in the Mid-Holocene period. Materials and methods In this work, the elevation, slope, slope direction, degree of riverside, and other indicators of Mid-Holocene settlement sites in the Weihai area were studied using the ArcMap software. Results The study produced three main findings. (1) The cultural sequence of Mid-Holocene settlement sites in the Weihai region is the Houli Culture, Dawenkou Culture, Longshan Culture, and Yueshi Culture. Compared with the Neolithic Culture sequence in Shandong Province, the Beixin Culture is absent, and the number of sites shows a right-tilted N-type trend, which increases, decreases, and then increases again. (2) The Mid-Holocene settlement sites in the Weihai region have some regularity in elevation, slope, slope direction, and degree of riverside. They are mainly distributed on flat terrain with a slope between 0° and 4° and an elevation greater than 30 m. In terms of slope direction, those settlement sites are mostly distributed on sunny slopes with directions of southeast, south, and southwest, which account for 58.1% of the total. For degree of riverside, all settlement sites are less than 1.5 km from the river. Discussion If these are facts, it will be inferred that the suitable nature conditions promoted the prosperity of Dawenkou Culture (6.3 4.6 ka BP) because of the arrival of the Mid-Holocene megathermal period which brought the relatively warm climate. In addition, the marine transgression incurring in 5.0 ka BP to 4.0 ka BP might be the main reason for the decline of Dawenkou Culture, and also the limitation of the development of Longshan Culture (4.6 4.0 ka BP) in the later period. What's more, human's dependence on nature conditions and the advanced thought of conforming to nature, seeking advantages and avoiding disadvantages have been developed, as the time passed. Conclusions Human activities of the Weihai area began to appear in the early Mid-Holocene (Houli Culture, 8.5 7.5 ka BP). With the arrival of the great warming period in the Mid-Holocene, the climate generally entered a warm period and was relatively mild. These natural conditions contributed to the Dawenkou Culture (6.3 4.6 ka BP). The transgression during 5.0 4.0 ka BP might be the main reason for the decline of Dawenkou Culture and also limited the development of the Longshan Culture (4.6 4.0 ka BP). Recommendations and perspectives Although the climate began to deteriorate from 4.0 ka BP, sea levels decreased, which provided a more spacious area and fertile soil for the development of humans in Weihai. Furthermore, the ancient river provided a solid foundation for the human culture in that period. All of these superior natural conditions were conducive to the development of Yueshi Culture (4.0 3.5 ka BP)