Expression and localization of importin 13 in fetal mouse brain tissues

由于真核细胞的基因复制、转录与蛋白质的表达翻译在细胞内分区进行，蛋白质及核酸等生物大分子跨核膜转运成为细胞中最重要的生物学功能之一。Importinβ家族成员是一类真核生物中广泛分布的核质转运受体蛋白。Importin13（Imp13）是这个家族成员之一，也是目前所知的哺乳动物细胞中少数能双向转运底物蛋白的蛋白质之一。目前研究已发现目前研究已发现Imp13的底物有hUbc9，RBM8（也称Y14），Pax3，Pax6，NF-YB/NF-YC，GR，myopodin，negativecofactor2，CHRAC-15/17，p12/CHRAC-17和eIF-1A。这些底物大多与发育有关，因此，...Since DNA replication and protein expression take part in the different compartments of an eukaryotic cell, nucleocytoplasmic trafficking plays an important role in the cell activities. Importin-β family members act as nuclear transport receptor in many nuclear transport activities．Interestingly, impotin13 (Imp13), a member of importin-β family, is found to be one bidirectional transport receptor ...学位：理学博士院系专业：生命科学学院生物学系_细胞生物学学号：2162006015331

Comparison of gene frequencies of 15 STR loci between patients with primary gastric adencarcinomas and the unrelated locals from Xiamen

目的比较15个短串联重复序列(STR)基因座基因频率在原发性胃腺癌患者和厦门地区正常人群中的分布,推测与胃腺癌相关的基因。方法123份血样采自本地区无癌家族史的健康人群,39份血样采自本地区胃腺癌患者。用聚合酶链反应(PCR)复合扩增结合四色荧光检测方法对血样DNA进行基因型分析,调查本地区健康人群和胃腺癌患者人群的基因频率分布,并根据两者的该15个基因座等位基因频率分布的差异性,推测易感连锁和抗性连锁的等位基因。结果厦门地区胃腺癌患者的TH01、vWA和FAG基因座的等位基因的分布与该地区健康人群比较差异有统计学意义(P<0·01)。在个别等位基因比较中,胃腺癌人群TH01-7的基因频率为0·0385,健康人群TH01-7的基因频率为0·2642,两者差异有统计学意义(P<0·01),相对危险度(RR)=0·1115;胃腺癌人群vWA-15基因频率0·0513,健康人群vWA-15的基因频率0·2927,两者差异有统计学意义(P<0·01),RR=0·1307;胃腺癌人群FAG-18的基因频率为0·1026,健康人群FAG-18的基因频率为0·0163,两者差异有统计学意义(P<0·01),RR=6·8998。结论TH01-7与胃腺癌相关联,其附近可能存在胃腺癌抗性基因;vWA-15附近有可能存在与胃腺癌相关的抗性基因;FAG-18与胃腺癌相关联,其附近可能存在胃腺癌易感基因。Objective To compare the gene frequencies of 15 STR loci between patients with primary gastric adencarcinomas and the unrelated locals from Xiamen in order to search for the genes correlated to the gastric adencarcinomas.Methods The control group consisted of 123 unrelated locals and the testing group was composed of 39 gastric adencarcinomas suffers. All genotypes of the sample DNA were analyzed by gene scan technology and multiplex PCR method with 4-colored fluorescence-labeled primers. All the polymorphic alleles of these 15 STR loci in unrelated healthy locals and patients with primary gastric adencarcinomas had been investigated. The sensitive or resistant genetic factors were inferred according to the statistical difference with distribution of allele frequencies.Results It showed that there were statistic differences (P<0.01)between controls and testing groups in allele frequencies of the three loci: TH01, vWA and FAG. The further exploration of the separated locus revealed that the gene frequency of TH01-7 in the gastric adencarcinomas suffers was 0.0385,but 0.2642 in the control group[P<0.01 and relative risk(RR)=0.1115];the gene frequency of vWA-15 in the gastric adencarcinomas suffers was 0.0513,but 0.2927 in the control group(P<0.01 and RR=0.1307);the gene frequency of FAG-18 in the gastric adencarcinomas suffers was 0.1026,but 0.0163 in the control group(P<0.01 and RR= 6.8998). Conclusions It is very possible that TH01 alleles may be associated with gastric adencarcinomas and it is possible that there is a resistant gene to gastric adencarcinomas near the region of TH01-7 locus; there is a resistant gene of gastric adencarcinomas near the region of vWA-15 locus; FAG alleles may be associated with gastric adencarcinomas and perhaps there is a sensitive gene of gastric adencarcinomas near FAG-18 locus

Study on the Application of 16 STR Loci in Paternity Examination and Individual Identification

将短串联重复序列应用于法医物证学案件中.用PCR复合扩增结合四色荧光检测及聚丙烯酰胺垂直电泳技术对一起碎尸案进行CSF1PO、D3S1358、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51、D21S11、FGA、PentaD、Pen taE、THO1、TPOX、vWA及Amelogenin性别位点的基因型检测,进行法医学亲子鉴定和个体识别.16个基因位点非父排除率为0.99999999999999996,个体识别率为0.99999999999999998.本方法及这些基因位点均实用于法医物证学个体识别.本方法鉴定快速,准确,结果分析标准化,使不同实验室的认定结果有了可比性.Short tandem repeat(STR)has been applied to forensic medicine human identification.We conducted paternity examination and individual identification of some fragments of a corpse using the PowerPlex 16System Kit purchased from the Promega Corporation . The 16 loci of D3S1358、THO1、D21S11、D18S51、Penta E、D5S818、D13S317、D7S820、D16S539、CSF1PO、Penta D、vWA、D8S1179、TPOX、FGA and sex locus Amelogenin are detected by the technique of multiplex PCR with 4-colour-fluorescent-labeled primers following the PAGE vertical electrophoresis. The combined mean probability of exclusion (P_E) and the individual discrimination power (D_P) are 0.999 999 999 999 999 96 and 0.999 999 999 999 999 98,respectively.It suggests that this technology and this multiplexing of 16 loci can be used in human identification and paternity test of forensic samples.It has been proved that the method mentioned above is rapid,accurate and standardized,adapting for comparison of the results of different laboratories

Sensitive and resistant genetic factors related to colorectal cancer in patients from Xiamen

目的:比较15个STR基因座基因频率在厦门地区大肠癌患者和正常人群中的分布,推测与大肠癌相关的基因．方法:应用PCR复合扩增结合四色荧光检测方法对血样DNA进行基因型分析,调查了本地区大肠癌患者人群和无关人群的基因频率分布,并根据二者的该15个基因座等位基因频率分布的显著性差异,推测易感连锁和抗性连锁的等位基因．结果:厦门地区大肠癌患者的D5S818(0．5200 vs 0,219 5,X2=36．69,P<0．01;RR=3．8521, P<0．05)、vWA(0．0500 vs 0．2927,X2=53．99, P<0．01;RR=0．1272,P<0．05)和FAG(0．09 vs 0．2439,X2=37．58,P<0．01:RR=0．3066, P<0．05)基因座的等位基因的分布与该地区健康人群有显著性差异,(P<0．01)．B组超微结构改变明显,而C组较B组超微结构有不同程度减轻．结论:D5S818-11附近可能存在大肠癌易感基因;vWA-15、FAG-23附近有可能存在与大肠癌相关的抗性基因．AIM: To compare the gene frequencies of 15 STR loci between patients with colorectal cancer and healthy people from Xiamen in order to search for the genes that related to the colorectal cancer. METHODS: The genotypes of the sample DNA were analyzed by multiplex polymerase chain reaction (PCR) combined with 4-colored fluorescence-labeled method. All the polymorphic alleles of these 15 STR loci in the unrelated healthy locals and patients with colorectal cancer were investigated. The sensitive or resistant genetic factors were inferred according to the statistical difference in the distribution of allele frequencies. RESULTS: There were statistical differences between the healthy controls and patients with colorectal cancer in allele frequencies of the three loci: D5S818 (0.520 0 vs 0.219 5, X2=36.69, P < 0.01; RR = 3.8521, P < 0.05), vWA (0.050 0 vs 0.292 7, X2=53.99, P < 0.01; RR = 0.127 2, P < 0.05), and FAG (0.09 vs 0.243 9, X2=37.58, P < 0.01; RR = 0.306 6, P < 0.05). CONCLUSION: It is very possible that there is a sensitive gene for colorectal cancer near the area of D5S818-11 locus, and there are resistant genes for colorectal cancer near the region of vWA-15 and FAG-23 locus

Study on expression and significance of tumor suppressor gene WWOX in hepatocellular carcinoma tissues by real-time fluorescent quantitative PCR

目的检测染色体普通型脆性位点FRA16D(16q23.3-q24.1)上WWOX(WW domain containing oxidoreductase)基因在原发性肝细胞癌(HCC)中的表达及意义。方法用RT-PCR、荧光定量PCR方法分别对40例原发性肝细胞癌癌组织(以下简称肝癌组织)、癌旁组织中WWOX基因的Δ6-8变异情况及其mRNA表达差异进行检测。结果WWOX mRNA在肝癌组织的表达(0.318±0.0559)显著低于癌旁组织(1.0),P<0.05;而且在肝癌组织中Δ6-8变异体的出现频率为20%(8/40),显著高于癌旁组织2.5%(1/40),P<0.05。结论WWOX基因的低表达、变异可能在肝癌的发生发展中起重要作用,并有可能作为肝癌的早期诊断指标之一。 【英文摘要】 Objective To evaluate the expression and significance of tumor suppressor gene(WWOX)in hepatocellular carcinoma(HCC)tissues.Methods Variant delta 6-8 of WWOX in patients with hepatocellular carcinoma was detected by RT-PCR and the expression of WWOX mRNA was examined by fluorescent quantitative PCR(FQ-PCR).Results The expression of WWOX mRNA in HCC patients(0.318±0.0559)was significantly lower than that in para-carcinoma tissue(P<0.05).Variant delta 6-8 was found in HCC and its frequency(20%,8/40)was sig..