Study on expression and significance of tumor suppressor gene WWOX in hepatocellular carcinoma tissues by real-time fluorescent quantitative PCR

Abstract

目的检测染色体普通型脆性位点FRA16D(16q23.3-q24.1)上WWOX(WW domain containing oxidoreductase)基因在原发性肝细胞癌(HCC)中的表达及意义。方法用RT-PCR、荧光定量PCR方法分别对40例原发性肝细胞癌癌组织(以下简称肝癌组织)、癌旁组织中WWOX基因的Δ6-8变异情况及其mRNA表达差异进行检测。结果WWOX mRNA在肝癌组织的表达(0.318±0.0559)显著低于癌旁组织(1.0),P<0.05;而且在肝癌组织中Δ6-8变异体的出现频率为20%(8/40),显著高于癌旁组织2.5%(1/40),P<0.05。结论WWOX基因的低表达、变异可能在肝癌的发生发展中起重要作用,并有可能作为肝癌的早期诊断指标之一。 【英文摘要】 Objective To evaluate the expression and significance of tumor suppressor gene(WWOX)in hepatocellular carcinoma(HCC)tissues.Methods Variant delta 6-8 of WWOX in patients with hepatocellular carcinoma was detected by RT-PCR and the expression of WWOX mRNA was examined by fluorescent quantitative PCR(FQ-PCR).Results The expression of WWOX mRNA in HCC patients(0.318±0.0559)was significantly lower than that in para-carcinoma tissue(P<0.05).Variant delta 6-8 was found in HCC and its frequency(20%,8/40)was sig..