Studies on Biology of Early Developmental Stages of Babylonia

东风螺(Babylonia)隶属软体动物门(Mollusca)、腹足纲(Gastropoda)、新腹足目(Neogastropoda)、东风螺科(Babyloniidae)，分布于中国东海、南海以及东南亚、日本和印度洋沿岸，因肉质鲜美、市场接受度高，而且生长速度快、养殖产量大，被认为是本世纪最具开发前景的优质海产腹足类之一。方斑东风螺和泥东风螺是我国常见的东风螺种类，对于其早期发育阶段的形态学以及多种组学进行研究，不仅在理论研究上具有科学意义，而且在生产实践上也具有重要的指导意义。 本研究采集了海南方斑东风螺、泰国方斑东风螺、海南方斑雌性与泰国方斑雄性杂交种、泰国方斑雌性与海南方斑雄性杂交种...The species in the genus Babylonia (Mollusca, Gastropoda, Neogastropoda, Babyloniidae) are considered to be the most promising economic marine gastropods in the present century, because of their wide distribution along most parts of Asian coastlines, their delicate flavor and their high market reception. Babylonia areolata and B. lutosa are two common species in China, which are good study materia...学位：理学博士院系专业：海洋与地球学院_海洋生物学学号：2242010015359

The Current Situation of Babylonia spp. Farming Industry in Hainan and Prospect Outlook

综述了我国海南省东风螺的养殖历史、养殖产业现状以及存在的问题。针对这些问题,提出了推进海南东风螺产业发展的一些建议。The history,current situation and problems in the farming of Babylonia spp.in Hainan Province were reviewed.Aiming at those problems,several suggestions for promoting Babylonia spp.farming industry development in Hainan were put forward.海洋公益专项(201405020); 2015年海南省科研院所技术开发专项——海南省热带海洋生物资源开发利用创新平台; 南海生物资源开发与利用协同创新中心项

3种养殖方式下沐浴角骨海绵生长状况的差异分析

沐浴角骨海绵（Spongia officinalis）主要分布于海南沿海，其骨架不由骨针而是由海绵硬蛋白组成，因而拥有良好的吸水性和柔性，在海洋生物材料开发领域具有较好前景。为了培育该地域特色的海绵品种，在海南省儋州水牛坡村潮间带海域（109.40° E, 19.87° N）开展了为期一年半的沐浴角骨海绵人工养殖，通过组织块的长、宽、高变化表征海绵的增长，分析了3种养殖方式下该海绵的生长状况差异。结果显示：在瓷砖法、穿绳法和弹簧法养殖方式下的角骨海绵均有较高的存活率，分别为80%，60%和50%，其中以瓷砖法最佳。在瓷砖法下，海绵组织块的平均长度增长率为67.1%，显著高于穿绳法和弹簧法（p<0.05），平均宽度增长率为63.5%，平均高度增长率为73.2%，可见瓷砖法相对于其他两种方式在存活率和增长率有较大优势，适宜于沐浴角骨海绵的养殖。此外，生长指标与海水温度的相关性分析结果表明，沐浴角骨海绵组织块的增长与水温之间的相关性不显著，增长速度不呈季节性变化。综上，在自然条件下获得的沐浴角骨海绵的第一手养殖资料有助于基础的海绵生物学研究，促进未来当地特色海洋经济的发展。海南省本级部门预算项目厦门南方海洋研究中心项目(17GYY008NF04

Study on Rapid Propagation Technique of Leather Corals

[目的]为海洋资源开发和生态环境修复等提供有效途径。[方法]探索肉质软珊瑚的人工快速扩繁方法。[结果]肉质软珊瑚具有快速的修复能力,能很快在肉质层的伤口处形成愈伤组织。通过该方法成功实现了肉质软珊瑚的快速人工扩繁。[结论]肉质软珊瑚内可能含有许多活性物质,具备一定的药物开发潜力。[Objective] The research aimed to provide an effective approach for developing ocean resources and repairing the ecological environment.[Method] The artificial method of rapid propagation of leather coral was explored.[Result] Leather coral was one species of soft corals,which had the properties of rapid regeneration and could develop the callus over the open wound.[Conclusion] Leather corals contained many active substances,so it had some medicinal development potential.2012年海南省科学事业费项目; 国家科技支撑计划项目(2012-BAC18B04-4); 国家海洋公益专项(201405020-5); 海南省科学事业费项目(KYYS-2014-50

A New Fabrication of Sponge Spicule

以漳浦海区常见的海绵为实验材料,介绍一种简易的海绵骨针制作方法.该方法应用铬酸溶液在塑料离心管中直接消化海绵,具有反应条件温和,操作简单,骨针完整、清晰等优点.A new fabrication of sponge spicule is introduced in this article.The main approach of this method as below: sponge specimen is added in a 1.5 mL plastic centrifuge tube,two or three drop of chromate solution is added into the tube,then the whole tube is incubated in 50~60℃ water for 2~3 min.After all the organism is digested,the spicules are washed three times using distilled water.After that the spicule is ready to observe.福建省自然科学基金(2006J0375)资

Grey Relation Analysis on Trace Elements Specturm of Astragalus

用 ICP- AES测定 1 5种黄芪中的 2 3种元素 ,以其含量为分析指标 ,用灰色关联方法研究了不同产地的黄芪在微量元素谱的亲疏关系 ,对不同品种的黄芪进行分类 ,为地道性中药材的研究提供简单的区分方法The content of 23 elements (TE) of Astragalus in 15differentspecies was determined by ICPAES. The content of TE in Astragalus was used as analytical index. The Grey Relation Analysis method was employed to describe close or distant relationship of the TE spectrum feature of Astragalus in different producing areas. The results show that the different species of Astragalus can be easily recognized by Grey Correlation Coefficient. It provides the discriminating method to study the genuine Chinese traditional medicine

Multivariate Analysis of Trace Elements in Serum Assistant Diagnosis of Nasopharyngeal Carcinoma

以鼻咽癌患者和对照组的 6 4例样本的微量元素含量及其比值作多元统计分析 ,建立判别方程、回归方程和主成分方程 .应用所建立的方程对 6 4个训练样本进行判别分类 ,分类准确率为 94% .此方法可应用于临床作为鼻咽癌的辅助诊断Trace element contents and their ratios of 64 samples of nasopharyngeal carcinoma patients and control groups were used to perform multivariate analysis to establish discriminatory equation, linear regression equation and principal component equation. The estabished equations were farther used to do discriminatory classification of 64 samples. By resubstitution test 94% accuracy is obtained. The method can be used in clinical as an assistant diagnosis of nasopharyngeal carcinoma

Cluster Analysis on Trace Elements in Astragalus of Different Sources

用ICP-AES测定15个不同产地的黄芪中的14种微量元素,以其含量为指标,进行因子分析,提取7个特征元素,并以特征指标进行样品的谱系聚类分析,把不同产地的黄芪分类,为地道性中药材的鉴别提供可靠方法elements(TE)in Astragalus of 15 different places of origins were determined by ICP-AES.The content of TE in Astragalus was used as analytical index.The factor analysis was applied to extract characteristic indices.7 characteristic indices were selected to perform hierarchical cluster analysis.The result showed that the Astragalus of different species and producing areas can be easily recognized and distinguished

Study of Computer Discriminant Analysis on Personal Urine TE SpectumClassfication of Personal Urine TE Spectrum in the High,Madial and Low Incidence of Esophgeal Cancinoma Districts

应用计算机对汕头地区的食管癌高中低发区的204份健康人尿样的微量元素谱进行单因素的均值方差分析和Q检验.用90例的观测值建立判别函数，应用该函数识别不同地区的微量元素谱，其判别效应经方差分析，P值＜0.01，回判准确率83.3%；对同时测定的114例观测值进行分类判别，识别的准确率达76.3%.The contents of 16 trace elements(TE) in 90 urinary samples collected from the high, medial and low incidence of esophageal cancinoma(EC) districts (Shantou area)were variance and multivariable stepwise discriminant analysed by computer.The stepwise discriminant equations were established by 8 variables.Applying the discriminant equation to the high,medial and low incidence.districts by resubstitution test 83.3% accuracy and to the other 114 samples accuracy is 76.3% Thus,these equations may provide a basis for prediction high,madial and low incidence of Esophageal cancer

Relation of Trace Elements with Synthesize of N nitroproline in Human Body

随机选取食管癌高低发区健康人60例，服用脯氨酸，采集晨尿，测定13种微量元素和亚硝基脯氨酸的含量，进行统计学分析：各指标的均值T检验、等级相关、剂量-效应及n-亚硝基脯氨酸相对生成率的u检验.结果表明：与n-亚硝基脯氨酸合成量呈正相关的元素是铍、铬、镍，呈负相关的有硒、钼、砷（P＜0.05~0.01）.healthy adult were randomly selected from the districts of high and low incidence of Esophageal cancer.They were asked to take proline and the 13 trace elements(TE) and N nitroproline (NPRO) in their urine of the next morning were determined. The contents of TE and NPRO were used to perform statistical analysis:t test of value of variable, Rank correlation, Dosage effect, and U test of relativity NPRO and TE.It was suggested that there is a negative correlation between contents of As, Mo, Se and NPRO synthesis in body which the positive one is for Be,Cr and Ni