一种新型草鱼呼肠孤病毒人工感染方法

研究从患病草鱼中新分离到一株草鱼呼肠孤病毒（Grass carp reovirus,GCRV）,对其进行了病毒纯化与电镜观察、基因组RT-PCR分型以及病毒量定量分析等,并在此基础上探索了一种新的病毒人工感染方法。取病鱼肌肉组织进行病毒纯化与电镜观察,观察到大量病毒粒子,直径在70—80 nm。病毒基因组RT-PCR扩增结果表明,该草鱼呼肠孤病毒新分离株属基因型Ⅱ型GCRV;通过绝对定量的方法,对病毒悬液的浓度进行了测定,为2.97×103 copy/μL。通过灌胃法,对3个组别的实验鱼分别感染不同浓度的病毒液,同时设置灌胃PBS的组别作为对照组。结果显示,3个实验组死亡率均在80%左右,而对照组仅出现一例死亡个体。实验组死亡个体体表发黑,腹部、鳍条基部以及鳃盖处均有明显的出血症状,为草鱼出血病的典型症状。随机选取死亡的个体进行RT-PCR检测,均能检测出Ⅱ型GCRV的条带。以上结果说明,灌胃法可以作为一种新的方法,用于草鱼的GCRV人工感染实验

非圆形喷洒域的喷头辅助装置

一种非圆形喷洒域的喷头辅助装置，其特点是采用中空的万向节结构，其上端连接喷头，下端连接供水管，在供水管外固定套接制有曲线状闭合凹槽的套环，其外活套有座套，座套上设有导杆置于凹槽内，在万向节外包套下端置有传动杆连至座套上端，本实用新型由于导杆在曲线状凹槽内滑行的同时有上下位移，能带动喷头腑仰角的变化，实现非圆形喷洒域的喷灌

一种新型草鱼呼肠孤病毒人工感染方法

研究从患病草鱼中新分离到一株草鱼呼肠孤病毒（Grass carp reovirus,GCRV）,对其进行了病毒纯化与电镜观察、基因组RT-PCR分型以及病毒量定量分析等,并在此基础上探索了一种新的病毒人工感染方法。取病鱼肌肉组织进行病毒纯化与电镜观察,观察到大量病毒粒子,直径在70—80 nm。病毒基因组RT-PCR扩增结果表明,该草鱼呼肠孤病毒新分离株属基因型Ⅱ型GCRV;通过绝对定量的方法,对病毒悬液的浓度进行了测定,为2.97×103 copy/μL。通过灌胃法,对3个组别的实验鱼分别感染不同浓度的病毒液,同时设置灌胃PBS的组别作为对照组。结果显示,3个实验组死亡率均在80%左右,而对照组仅出现一例死亡个体。实验组死亡个体体表发黑,腹部、鳍条基部以及鳃盖处均有明显的出血症状,为草鱼出血病的典型症状。随机选取死亡的个体进行RT-PCR检测,均能检测出Ⅱ型GCRV的条带。以上结果说明,灌胃法可以作为一种新的方法,用于草鱼的GCRV人工感染实验

可同时提供不同压力的灌溉装置

 一种可同时提供不同压力的灌溉装置，是在压力水箱上设置有2—5个出水管，除在一个上安装普通阀，其余出水管上安装减压阀。通过一台水泵与多个减压阀的组合，可使一台灌溉设备同时满足不同水压的需求，便于设备优化配置，可以节约资金和材料能源消耗，降低故障率和养护费用，适用于农田和园艺的节水灌溉

“西油东送”工程甘肃段水土流失防治研究/Soil and Water Loss Control Along Gansu Pipeline in West to East Petroleum Transportation Project[J]

中国石油西部成品油管道是目前国内运输距离最长的输油管道之一。管道铺设过程中,大量采用机械施工,路堑开挖,路堤填筑,取土采石和架桥砌涵等,破坏了公路沿线原生地貌及植被,扰动了表土结构,致使土体抗蚀能力降低。在总结工程区自然情况基础上,分析了＂西油东送＂工程甘肃段的水土流失问题和其对生态环境的影响。结果认为管道工程防护类型必须坚持＂三同时＂原则,并应该采取如下防治方针：（1）综合治理与综合开发相结合;（2）科学配置防治措施;（3）防止新的水土流失发生

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies