虎伯寮南亚热带雨林自然保护区的竹类资源及开发利用

福建省南靖县虎伯寮南亚热带雨林自然保护区的竹类资源丰富 ,有 8属 2 0种 3变种 5栽培型 ,其中梅花毛竹 ( Phyllostachys heterocycla cv.obtusangula)为福建竹类新分布 ;主要的竹林类型有毛竹林、麻竹林、绿竹林、石竹林、单竹林。竹类资源的开发利用具有广阔的前

厦门市乙型肝炎患者病毒基因型的分析

目的:采用多对型特异性引物,通过巢式PCR法检测厦门市乙型肝炎患者血清中乙型肝炎病毒(HBV)基因型的分布情况.方法:收集250例HBV感染患者血清,提取血清中HBV DNA作为模板,设计HBV前S1基因和S基因中区域内设计出10条内外引物,并将其中8条型特异性内引物分成A,B两组,分别扩增A,B,C和D,E,F型HBV,然后将第2轮PCR产物以用30g/L琼脂糖进行电泳,根据PCR产物电泳显示的产物长度判定HBV基因型,以了解厦门HBV基因型分布情况.结果:共120例确定了HBV基因型.患者群中慢性乙型肝炎90例,占75.0%,急性乙型肝炎、肝炎肝硬化、原发性肝癌分别占5.8%(7/120)、6.7%(8/120)和12.5%(15/120).分型结果:B型58例(48.3%)、C型30例(25.0%),B/C混合型32例(26.7%).HBeAg阳性患者中B基因型占63.8%,B/C型混合感染21.9%;抗-HBe阳性患者中以B/C型混合感染68.8%,B型25.9%,HBeAg阳性组与抗-HBe组之间比较发现B型和B/C混合型之间(P<0.05).结论:厦门乙型肝炎患者HBV基因型以B型为主,B/C混合感染是一个值得重视的问题

Enhanced bioconversion of hydrogen and carbon dioxide to methane using a micro-nano sparger system: mass balance and energy consumption

Simultaneous CO2 removal with renewable biofuel production can be achieved by methanogens through conversion of CO2 and H2 into CH4. However, the low gas–liquid mass transfer (kLa) of H2 limits the commercial application of this bioconversion. This study tested and compared the gas–liquid mass transfer of H2 by using two stirred tank reactors (STRs) equipped with a micro-nano sparger (MNS) and common micro sparger (CMS), respectively. MNS was found to display superiority to CMS in methane production with the maximum methane evolution rate (MER) of 171.40 mmol/LR/d and 136.10 mmol/LR/d, along with a specific biomass growth rate of 0.15 d−1 and 0.09 d−1, respectively. Energy analysis indicated that the energy-productivity ratio for MNS was higher than that for CMS. This work suggests that MNS can be used as an applicable resolution to the limited kLa of H2 and thus enhance the bioconversion of H2 and CO2 to CH4

Effects of nanobubble water on the growth of Lactobacillus acidophilus 1028 and its lactic acid production

Nanobubble water (NBW) has been applied in various fields due to the unique properties of nanobubbles (NBs) including long-term stability, negative zeta potential and generation of free radicals. In this study, the performance of four kinds of NBW from different gases (air, N2, H2, and CO2) in addition to deionized water (DW) were investigated and compared in terms of the growth of the probiotic Lactobacillus acidophilus 1028. The NB density, size distribution, zeta potential, pH and dissolved oxygen (DO) of the NBW were firstly investigated. Results indicate that N2-NBW had the highest absolute value of zeta potential and NB density (−25.3 ± 5.43 mV and 5.73 ± 1.0 × 107 particles per mL, respectively), while the lowest was detected in CO2-NBW (−6.96 ± 2.36 mV and 3.39 ± 1.73 × 107 particles per mL, respectively). With the exception of CO2-NBW, all the other types of NBW showed promotion effects on the growth of the strain at the lag and logarithmic phases. Among them, N2-NBW demonstrated the best performance, achieving the highest increase ratio of 51.1% after 6 h cultivation. The kinetic models (Logistic and Gompertz) indicate that the culture with N2-NBW had the shortest lag phase and the maximum specific growth rate when compared to the H2-NBW and DW groups under the same cultivation conditions. Preliminary analysis on the mechanisms suggested that these effects were related to the properties (zeta potential and density) of the NBs, which might affect the transport of substances. This study suggests that NBW has the potential for promoting the production efficiency of probiotics via fermentation

核因子-κB信号转导途径的调节研究进展

在静息状态时 ,核因子 κB((nuclearfactorκB ,NF κB)通常与其抑制物以无活性的复合物形式存在于胞浆 ,当受到胞外信号刺激时 ,通过一个或多个信号转导途径 ,激活一系列激酶 ,迅速从胞浆易位到胞核 ,调控相应靶基因的表达。目前发现它是免疫和炎症反应的主要调节因子 ,也参与胚胎发育、细胞周期的调控、细胞凋亡的调节 ,NF κB的过度表达与多种疾病的免疫病理机制有关。近年来许多学者深入探讨了NF κB信号转导途径的调节机制 ,使得许多疾病的发病机制得以进一步阐明 ,并最终为其治疗提供了理论基

氧传递速率对脱硫菌Pseudomonas delafieldii R-8生长和脱硫的影响

利用高脱硫活性德氏假单胞菌Pseudomonas delafieldii R-8为研究对象,考察了装液量对细胞生长及所得细胞脱硫活性的影响;并以正十二烷为模拟油相,测定了油水相生物脱硫过程中的体积氧传质系数。研究结果表明,装液量的多少不仅影响细胞生长,同时影响所得细胞的脱硫活性。装液量越少,细胞生长越好。采用500 ml三角瓶培养时,装液量为150 ml时,所得细胞的脱硫效果最佳。鼓泡曝气反应器中经过反应10h后体积氧传质系数k_1a达到最大值0.687 min~(-1),比脱硫活性从0增加到11 mg(DBT)/g

effectofsilverdepositiononphotocatalyticactivityoftio2

The Ag/TiO2 photocatalyst with varying Ag loadings was prepared by the photodeposition method and its photocatalytic activity was tested using phenol degradation as the model reaction. Transmission electron microscopy and diffuse reflectance spectroscopy (DRS) were used to observe the distribution and size of silver clusters on the TiO2 surface and to determine the optical characteristics of Ag/TiO2, respectively. The results showed that a suitable Ag loading could greatly enhance the TiO2 photocatalytic activity. Ag clusters were formed on the TiO2 surface and the size of Ag clusters increased with the increase in Ag loading. The DRS results indicated that silver deposition had no effect on the optical characteristics of the catalyst lit the UV region. The relationship between the Ag loading and the photocatalytic activity of Ag/TiO2 was discussed

effectofsilverdepositiononphotocatalyticactivityoftio2

The Ag/TiO2 photocatalyst with varying Ag loadings was prepared by the photodeposition method and its photocatalytic activity was tested using phenol degradation as the model reaction. Transmission electron microscopy and diffuse reflectance spectroscopy (DRS) were used to observe the distribution and size of silver clusters on the TiO2 surface and to determine the optical characteristics of Ag/TiO2, respectively. The results showed that a suitable Ag loading could greatly enhance the TiO2 photocatalytic activity. Ag clusters were formed on the TiO2 surface and the size of Ag clusters increased with the increase in Ag loading. The DRS results indicated that silver deposition had no effect on the optical characteristics of the catalyst lit the UV region. The relationship between the Ag loading and the photocatalytic activity of Ag/TiO2 was discussed

Inhibitory effect of ARHI on pancreatic cancer cells and NF-kappa B activity

Key Science Research Project Natural Science Foundation of the Xiamen Health Bureau [3502z20077038]The aim of this study was to investigate the effect of aplasia ras homolog member I (ARHI) on proliferation, apoptosis and the cell cycle in the pancreatic cancer cell line PANC-1. The study also aimed to examine the effect of ARHI on the activity of the nuclear factor (NF)-kappa B and to determine whether ARHI acts as a tumor suppressor in the development of pancreatic cancer by inhibiting the activity of NF-kappa B. A pIRES2-EGFP-ARHI vector, constructed by reverse transcrition (RT)-PCR, was transiently transfected into the PANC-1 cells and analyzed for the expression of the ARHI protein by western blotting. A MTT assay was used to quantify cell proliferation, and apoptosis was analyzed by flow cytometry. The NF-kappa B signaling pathway, specifically the pathway using the nuclear phosphorylated p65 isoform, was analyzed by western blotting. Expression of the ARHI protein was detected by western blotting subsequent to the PANC-1 cells being transiently transfected with the pIRES2-EGFP-ARHI construct. Cell proliferation was strongly inhibited in the PANC-1 cells transfected with pIRES2-EGFP-ARHI. The cell cycle assays indicated an increase in the number of cells at the G0/G1 phase and a decrease in the cells at the S phase, but the difference was not significant (P > 0.05). Time course studies also indicated a marked increase in the apoptotic index following transient transfection, as well as a gradual decrease in the expression of the nuclear phosphorylated p65 protein. ARHI acts as a tumor suppressor by downregulating the NF-kappa B signaling pathway, which results in the inhibition of cell proliferation, apoptosis and the cell cycle in the pancreatic tumor PANC-1 cell line