间歇式轴向压应力对组织工程骨种子细胞的黏附增殖与成骨分化促进作用的研究

目的探究间歇式轴向压应力对组织工程骨种子细胞黏附、增殖与成骨分化能力的影响。方法构建表达绿色荧光蛋白的兔骨髓间充质干细胞(rBMSCs)作为示踪种子细胞,运用旋转细胞培养仪将松质骨支架和种子细胞共培养7 d获得组织工程骨(TEB),实验组在第7～14天施加大小10 N、频率1 Hz、4 h/d的间歇式轴向压应力刺激,对照组常规培养,14 d后胰酶消化法获取两组种子细胞并比较其黏附、增殖和成骨分化能力。采用两组独立样本t检验进行统计学分析。结果 (1)流式细胞术显示rBMSCs被成功提取分离。(2)倒置荧光显微镜及扫描电镜显示TEB中种子细胞与支架相容性良好。(3)活体荧光成像系统及扫描电镜显示应力刺激组种子细胞的生长状况要优于非应力刺激组,前者平均荧光密度及细胞数/500倍视野均大于后者,差异均具有统计学意义(平均荧光密度:(3.75±0.34)×108 vs(2.91±0.22)×108,t=2.90,P=0.04;细胞数/500倍视野:30.50±4.43 vs 21.00±5.13,t=3.14,P=0.01)。(4)细胞黏附实验显示,应力刺激组种子细胞的75﹪细胞贴壁时间短于非应力刺激组,两组时间分别为(3.00±0.41) h、(13.33±1.70)h,差异具有统计学意义(t=8.20,P <0.01),前者的最终细胞贴壁率高于后者(99.97﹪±0.34﹪vs 85.83﹪±1.18﹪),差异具有统计学意义(t=11.31,P <0.01)。(5) CCK-8检测显示,在培养第48～96 h,应力刺激组种子细胞的增殖能力优于非应力刺激组,将两者的450 nm吸光度值在第48小时(0.49±0.02、0.40±0.02)、72 h(0.76±0.07、0.64±0.04)和96 h(1.58±0.07、1.34±0.13)分别进行比较,差异均具有统计学意义(t=5.15、2.57、2.86,P均<0.01)。(6)在成骨诱导14 d后,应力刺激组种子细胞的ALP和Ca结节染色阳性率要强于非应力刺激组:两组ALP染色阳性率分别为26.73﹪±4.56﹪、16.68﹪±3.89﹪,差异具有统计学意义(t=3.33,P=0.03);两组Ca结节染色阳性率分别为41.81﹪±3.56﹪、27.40﹪±2.35﹪,差异具有统计学意义(t=3.68,P=0.02)。结论间歇性轴向压应力可促进组织工程骨种子细胞的黏附、增殖与成骨分化

Developing On-site,Quick Screening Platform for Artificial Pigments in Food Using Surface-enhanced Raman Spectroscopy

已有研究表明食品加工过程中添加的人工合成色素不仅不能提供营养物质,而且可能是导致小儿多动症的来源之一,影响儿童智力发育.基于表面增强拉曼光谱(SERS)原理和便携式拉曼光谱仪,提出了一种非定向的现场快速筛查方法.该方法只需对疑似含有人工合成色素的固体或液体状食品样品进行简单前处理,即可进行SERS检测.样品前处理和检测的总时长不超过15 min,检出质量浓度在1 mg/L水平,可有效满足政府职能部门的现场执法需求.除具有快速、方便、灵敏度高等特点之外,该检测方法的最大优势在于实现了未知样品的现场非定向测试:在同一种前处理过程和检测方法下,可对食品中常添加的亮蓝、胭脂红、日落黄、柠檬黄、苋菜红和诱惑红6种人工合成色素进行快速鉴定和半定量分析.As widely used additives in food processing,artificial pigments have no any nutrition but are potential sources of hyper-activity and affect the intellectual development of children.On the basis of surface-enhanced Raman spectroscopy( SERS) and portable Raman instrument,we developed a non-targeting,on-site and quick screening platform for artificial pigments in food matrix. SERS measurement could be carried out after the food matrix( either in solid or liquid states) being simply pretreated for 15 min.The detectable concentration is as low as 1 mg / L,a level meeting the demand of the on-site enforcement by the government.Besides the advantages of quick,easy-on-going,and high sensitivity,the most distinguished point of SERS is the non-targeting qualitative on-site detection for the typical pigment additives,including brilliant blue,carmine,sunset yellow,lemon yellow,amaranth and allura red,et al.,while both the pretreatment procedure and the SERS detection remain the same.国家自然科学基金(21473140);; 福建省高校产学合作项目(2016Y4012);; 中央高校基本科研业务费专项(2072016011

Developing fast laboratory screening platform for sulfate dioxide in food by surface-enhanced Raman spectroscopy

基于表面增强拉曼光谱(SERS)的指纹图谱的高能量分辨率,以位于~630; cm~(-1)亚硫酸根的特征峰为定性和定量依据,本文开展了食品中滥用和非法添加二氧化硫的直接检测研究:一种检测流程实现了不同食品基质中二氧化硫的; 高灵敏检测,最低检出浓度达1 mg; kg~(-1)的水平.针对实际样本的传统蒸馏法前处理流程的一些不足以及SERS检测的指纹图谱优势,本文进行了三方面显著改进:; (1)碳酸钙和沸石取代氮气以提高二氧化硫的挥发提取效率; (2)草酸取代盐酸酸化蒸馏溶液,以有效避免挥发性强酸对操作环境的危害;; (3)氢氧化钠溶液代替乙酸铅溶液作为吸收液,以防止二次污染.环境友好、灵敏度高和不受基质干扰等特点使得本方法有望取代经典蒸馏法和比色法,用于食品; 中非法添加二氧化硫类添加剂的快速高效筛查.The direct detection of sulphur dioxide has been realized in various; food matrixes with high sensitivity and high selectivity, on the basis; of the integration of the high energy resolution of the finger-print; spectrum of surface-enhanced Raman spectroscopy and the universal but; easy-on-going pretreatment procedure. The characteristic peak of sulphur; dioxide at ~630 cm~(-1) was applied as the qualitative and quantitative; standard, which displayed a lowest detectable concentration at the 1 mg; kg~(-1) level for the spiked food samples. The key point of the high; sensitivity and selectivity is the effective pretreatment born out of; the standard distillation one, which has been improved in the three; parts. (1) Using CaCO_3 and Zeolite instead of N_2 gas as the bubbling; reagent. (2) Using oxalic acid as the acidic distillation solution to; eliminate the hazards from acid volatilization, such as hydrochloric; acid. (3) Using diluted sodium hydroxide solution as the absorption; reagent instead of lead acetate solution to avoid the secondary; pollution. With the three distinguished advantages of environment; friendly, high sensitivity and free of matrix interference, the proposed; method has great potential to replace the traditional ones for the fast; screening of the illegal or abused sulphur dioxide in food.国家自然科学基金; 福建省高校产学合作项目; 中央高校基本科研业务费专项资

Relation between Sense of Self-Affirmation and Interpersonal Relationship among Elementary Schoolchildren

departmental bulletin pape

SDF-1α/CXCR4信号通路在轴向应力刺激促进骨再生中的作用研究

目的 通过轴向应力刺激促进骨再生,观察基质细胞衍生因子1α/趋化因子CXC亚族受体4(stromal cell-derived-factor-1α/cysteine X cysteine receptor 4,SDF-1α/CXCR4)信号通路变化,探讨轴向应力刺激促进骨再生的机制..