Application of Gel-based Proteomic Approachs in Prostate Cancer Research

基于凝胶蛋白质组学技术提供了蛋白质的定量和定性信息，在蛋白质组学研究中，尤其是在临床穿刺样品的分析中起着核心作用。本文针对基于凝胶蛋白质组学中技术性强、手工依赖性大、对分析质量产生重要影响的3个环节，即双向电泳（2DE）、蛋白质显色及蛋白质胶内酶解，进行方法学研究；同时应用基于凝胶蛋白质组学技术对前列腺穿刺样品（PNBX）进行前列腺癌的蛋白质组学研究。不仅建立了适用于不同生物样品的高重复性、高分辩率的基于凝胶蛋白质组学分析平台，而且首次揭示了蕴含在PNBX中的蛋白质组信息，发现了一些潜在的前列腺癌分子标记物。 在方法学研究部分，本文首先建立并且比较分析两种双向电泳体系ISO-DALT和IPG...Gel-based proteomics generates qualitative and quantitative protein behavioral data, and as such it plays a central role in proteomic studies, particularly in the analysis of clinical biopsy specimens. In this study, efforts focused on 2DE, protein visualization, and protein in-gel digestion were made to ensure success of gel-based proteomics. With the optimized techniques, I conducted the proteom...学位：理学博士院系专业：生命科学学院生物化学与生物技术系_生物化学与分子生物学学号：2005140314

佳辐占和广陆矮4号水稻叶片蛋白质的双向电泳比较研究

应用双向电泳技术研究比较两种不同品质水稻(佳辐占和广陆矮4号)叶片的蛋白质组分的差异.两种水稻叶片提取的蛋白质的双向电泳图谱斑点清晰,形态规则.采用考马斯亮蓝染色获得约400个斑点,银染图谱约800个斑点.两种水稻的蛋白质图谱考马斯亮蓝染色图谱存在5个显著差异点,银染图谱存在11个显著差异点

纳米颗粒对极化聚合物的非线性驰豫特性的影响研究

以K-M模型和光散射理论为基础,建立了纳米颗粒掺杂改性的PMMA聚合物材料的紫外散射模型,推导出了考虑入射波偏振的理论计算公式,解决了在非线性光学性能的抗老化与聚合物波导的加工之间所存在的矛盾。利用MATLAB编制了基于Mie算法的程序,分析了收敛条件,并对体系纳米粒子的粒径与后向散射系数、吸收系数的定量关系进行理论计算。仿真结果表明,在波长约200-400nm范围内,最佳抗老化效果的粒径集中在20-50nm之间。利用偏振对于粒子散射影响较大的特点,提出用带偏振片的紫外灯来进行波导光刻加工的设想,确定了最佳掺杂颗粒半径35-40nm之间的理想范围。最后,将仿真结果与实验结果进行了对比分析,证实了散射模型和仿真过程的可靠性。“福建省科技计划重点项目(2007H0036)”提供资

神经导航在颅内胶质瘤显微手术的应用

目的　评价神经导航在颅内胶质瘤显微手术的应用价值。方法　应用神经导航系统进行颅内胶质瘤显微手术 30例。结果　用神经导航系统进行胶质瘤及其周围解剖结构定位准确 ,平均注册误差 (2 .5± 0 .4 )mm。术后近期复查CT或MR证实胶质瘤全切率 83.3% ,手术时间和住院日较常规开颅手术缩短。结论　 (1)神经导航定位精确 ,有助于手术计划的精确实施。 (2 )实时导航颅内胶质瘤显微手术 ,有助于达到微侵袭效果和提高胶质瘤全切除率。 (3)摸索一套减少靶点漂移的经验

Design and Fabrication of Al_2O_3/SiO_2 Double-Layer Antireflection Coatings on 4H-SiC Substrate

在4H-SiC基底上设计并制备了Al2O3/SiO2紫外双层减反射膜,通过扫描电镜(SEM)和实测反射率谱来验证理论设计的正确性。利用编程计算得到Al2O3和SiO2的最优物理膜厚分别为42.0nm和96.1nm以及参考波长λ=280nm处最小反射率为0.09%。由误差分析可知,实际镀膜时保持双层膜厚度之和与理论值一致有利于降低膜系反射率。实验中应当准确控制SiO2折射率并使Al2O3折射率接近1.715。用电子束蒸发法在4H-SiC基底上淀积Al2O3/SiO2双层膜,厚度分别为42nm和96nm。SEM截面图表明淀积的薄膜和基底间具有较强的附着力。实测反射率极小值为0.33%,对应λ=276nm,与理论结果吻合较好。与传统SiO2单层膜相比,Al2O3/SiO2双层膜具有反射率小,波长选择性好等优点,从而论证了其在4H-SiC基紫外光电器件减反射膜上具有较好的应用前景。Al2O3/SiO2 double-layer UV antireflection coatings were designed and fabricated on 4H-SiC substrate,and the validity of theoretical design was further verified by scanning electron microscope (SEM) and reflection spectrum. The optimal physical thickness of Al2O3 and SiO2 is 42.0 nm and 96.1 nm respectively by programming calculation. And then the minimum reflectance of 0.09% is obtained at reference wavelength λ=280 nm. According to error analysis,keeping the sum of double-layer thickness consistent with theoretical value is helpful to reduce the reflectance. In addition,the refractive index of SiO2 should more accurate and the refractive index of Al2O3 should be controlled close to 1.715 in the experiment. Al2O3/SiO2 double-layer coatings were deposited on 4H-SiC substrate by electron beam evaporation and the physical thickness is 42 nm and 96 nm respectively. SEM images show that the deposited layers and the substrate perform good adhesion to each other. The practical minimum reflectance is 0.33% at λ=276 nm which is close to theoretical value. Compared with conventional SiO2 single layer,Al2O3/SiO2 double-layer coatings show low reflectance and better wavelength selectivity. These results make the possibility for 4H-SiC based UV optoelectronic devices with Al2O3/SiO2 films as antireflection coatings

Biological nitrogen fixation in the upper water column in the south Taiwan Strait during summer 2011

2011年6—7月,利用15n2示踪法实测了台湾海峡南部海域的生物固氮速率,结合温度、盐度、天然颗粒物氮同位素组成的分布,分析并讨论了影响研究海域生物固氮速率的环境因素。结果表明,夏季台湾海峡南部海域的生物固氮速率介于168—1080 nMOl M-3d-1之间,平均为537 nMOl M-3d-1,较高的生物固氮速率大多出现在次表层水体中。研究站位的积分固氮速率变化范围为11—40μMOl M-2d-1,平均为23μMOl M-2d-1。积分固氮速率的空间变化与不同水团的影响有关,在受黑潮水影响的海域,生物固氮速率较高,而在上升流和受河流冲淡水影响的海域,生物固氮速率较低,说明较低的水温及较高的无机氮营养盐可能会抑制研究海域的生物固氮作用。研究海域天然颗粒物δ15n与生物固氮速率之间呈现良好的负相关关系,表明天然颗粒物氮同位素组成可定性指征研究海域生物固氮作用的强弱。Biological N2fixation in marine environments is a major component in the ocean nitrogen budget and plays an important role in global carbon cycles through the sequestration of atmospheric carbon dioxide and production of marine organic matter.N2fixation could be regulated by the abundance and chemical speciation of nutrients and many trace elements in seawater.Recent studies have revealed that N2fixation is much more widespread in marine environments than previously thought.However,little is known about the N2fixation in the Taiwan Strait,especially on N2fixation rates,and their relationship with environmental parameters.The major objectives of this study were to determine the N2fixation rates and their spatial distributions and to explore major physicochemical controlling factors in the south Taiwan Strait.During June and July 2011,seawater samples were collected from ten stations at two transects in the south Taiwan Strait for the measurements of N2fixation rate using the15N2tracer assay.Particulate nitrogen and its isotopic composition were measured with an elemental analyzer(Carlo Erba NC 2500) coupled with a Finnigan MAT DeltaplusXP isotope ratio mass spectrometer.Reproducibility of nitrogen isotope measurements(in terms of δ15N) was within 0.2‰.Our results showed that N2fixation rates in the south Taiwan Strait ranged from 168—1080 nmol m-3d-1with an average of 537 nmol m-3d-1.Most of the high rates were observed at subsurface layers.The depth-integrated N2fixation rates were 11—40 μmol m-2d-1with an average of(23±10) μmol m-2d-1.The distribution of the N2fixation rates showed regional variations with influence from water masses with distinctive temperature and salinity.Higher N2fixation rates were mostly observed in the regions influenced by the Kuroshio,with an average of 31 μmol m-2d-1,while lower rates occurred in the upwelling and river plume regions with an average of 15 μmol m-2d-1.This spatial distribution pattern indicated that biological N2fixation was largely impeded by the low temperature and the high concentration of dissolved inorganic nitrogen in the south Taiwan Strait.The contribution of N2fixation in the study area could be further quantified based on the δ15N signatures of suspended particles which could be significantly depleted during N2fixation.Indeed,a negative correlation between the δ15N signatures of suspended particles and N2fixation rates was observed regardless of using all data points or depth-averaged values within the water column.This indicated that15N-depleted particles were largely derived from the enhanced N2fixation,supporting the use of nitrogen isotopic composition(δ15N) of suspended particles as a potential indicator of N2fixation in the south Taiwan Strait.Further studies are needed to better elucidate the relationship between N2fixation rates and limiting elements and their chemical speciation,and thus the physical and biogeochemical controls on N2fixation in the south Taiwan Strait.国家自然科学基金资助项目(41125020;41076043;41206062); 国际海域资源调查与开发“十二五”项目(DY125-13-E-01

表面增强拉曼光谱研究硫脲及其衍生物在银电极上的共吸附行为

用表面增强拉曼光谱技术（ＳＥＲＳ）研究了ＨＣｌＯ４介质中硫脲（ＴＵ），一甲基硫脲（ＭＴＵ）和烯丙基硫脲（ＡＴＵ）在银电极表面的共吸附行为．首次报道了它们的混合物在银电极表面上竞争共吸附随电极电位变化的行为以及在所研究的共吸附体系中作为支持电解质的弱吸附ＣｌＯ－４离子被诱导物理共吸附的现象．作者联系地址：江西师范大学化学

Expression and Localization of Nucleophosmin During HMBA-induced Differentiation in Human Hepatocarcinoma SMMC-7721 Cells

To explore the existence and distribution of nucleophosmin in the nuclear matrix and its co-localization with the other related gene products following HMBA treatment in the human hepatocarcinoma SMMC-7721 cells,the nuclear matrix of SMMC-7721 cells was extracted pre/post HMBA induced differentiation.2D PAGE proteomics analyses showed that nucleophosmin existed in the fractions of nuclear matrix proteins and was down-regulated after HMBA treatment with further confirmation by Western blot analysis.The immunofluorescence observation revealed that nucleophosmin located in the nuclear matrix,HMBA treatment altered its expression level and distribution profile.The co-localization of nucleophosmin with cancer-related genes and the products of oncogenes or tumor repression genes,including c-fos,c-myc,p53 and Rb,using laser scanning confocal microscopy,were evaluated,and substantial differences were observed following HMBA treatment.The results implies that nucleophosmin,as a nuclear matrix protein,the level of its expression and the colocalization with cancer-related gene products may play an important role during the differentiation of SMMC-7721 cell.国家自然科学基金资助项目(No.30470877)~