THE APPLICATION OF ISOTOPE RATIO ANALYSIS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETER

本论文共由五章组成：1、前言；2、电感耦合等离子体四极杆质谱同位素稀释法应用研究;3、铅同位素比值在中药产地判别中的应用;4、大洋多金属结核研究开发中的分析技术；5、大洋多金属结核中稀土元素的等离子质谱测定方法及其逐层形态分析。 在前言中，从电感耦合等离子体质谱进行同位素比值分析具有的优点出发，讨论了影响ICP-MS同位素比值测定的准确度和精密度的因素及解决办法，综述了电感耦合等离子体质谱的同位素分析应用研究现状，最后比较了几类用于同位素比值分析的ICP-MS仪器。 第二章发展了同位素稀释电感耦合等离子体四极杆质谱法（ID-ICP-QMS），利用所建立的方法测定了植物与人发中的铅。考察和讨...This disserstation consists of five chapters: 1、Preface；2、The Study of Isotope Dilution Inductively Coupled Plasma Mass Spectreometry（ID-ICP-MS）and Its Application. 3、The Application of Lead Isotopic Fingerprints to the Origination Identification of Traditional Chinese Medicines; 4、The Analytical Methods in the Research and Exploitation of Pacific Nodules；5、Determination of Rare Earth Elements in ...学位：工学硕士院系专业：化学化工学院化学系_环境科学学号：19982504

基于大类招生培养模式的化学人才培养方案和课程体系设计

大类招生培养是以学生为本的培养模式的改革。在此背景下我校化学学科制订了以满足学生多样化需求为导向的人才培养方案,构建了一个包括公共基础课、通识课、学科通修课、多层次的实践类课程、模块化的专业方向性课程以及突显学科优势的选修课在内的课程体系,并完善了配套的保障机制。国家基础科学人才培养基金项目(J1310024

高首效富镍正极材料LiNi0.6Co0.2Mn0.2O2的合成及电化学性能研究

采用共沉淀—高温固相烧结的方法合成了富镍正极材料LiNi0.6Co0.2Mn0.2O2（简称NCM622）,通过X射线粉末衍射（XRD）/Rietveld精修法、扫描电子显微镜（SEM）及电化学测试,对不同温度下合成材料的结构、形貌、电化学性能进行表征.结果表明,800℃下NCM622阳离子混排程度最低（1.97%）,首周库仑效率高达92.2%,100周容量保持率为81.4%.国家自然科学基金项目(No.21233004,No.21428303)资

数字微流控技术及其在生物分析中的应用

数字微流控技术是一种基于微电极阵列来实现离散液滴精确控制的新型液滴操纵技术。这种基于介电润湿现象实现的液滴电操纵体系,相比于传统微流控芯片具有自动化、可寻址、可动态配置、易集成等特点。该文介绍了数字微流控技术液滴驱动原理,总结了芯片的结构和常用的制作方法,举例阐述了现阶段该技术在生物分析化学领域的应用,并对其应用前景做了展望。国家自然科学基金资助项目(21735004,21435004,21775128,21705024,21521004);;长江学者和创新研究团队项目(IRT13036

A cyclic enzymatic amplification method for sensitive and selective detection of nucleic acids

Based on Exonuclease III (Exo III) and displacing probes, we have developed a Cyclic Enzymatic Amplification Method (CEAM) for sensitive and selective detection of nucleic acids. In this design, the displacing probe is non-fluorescent on its own and cannot be digested by Exo III until displacement hybridization by a target sequence, leading to release of free non-quenched fluorophore. Because a single target sequence can lead to the release and digestion of numerous fluorophore strands from the displacing probe, a remarkable signal amplification is achieved. With this method, DNA can be detected in the picomolar range with a high selectivity and within less than 20 min.NSFC [20805038]; MOE [200803841013]; National Basic Research Program of China [2007CB935603, 2010CB732402

表面电性可控磁珠微流控芯片在DNA提取中的应用

制备了表面电性可控的氨基化SiO2@Fe3O4磁性复合微球,采用激光刻蚀和热压键合的方法制作了聚甲基丙烯酸甲酯（PMMA）材质的DNA固相萃取芯片,将磁珠灌注于芯片通道中,借助永磁铁固定并控制磁珠,将磁珠芯片应用于人类全血中的基因组DNA提取,优化了提取实验条件,并对提取产物进行凝胶电泳和PCR分析。实验结果表明,磁珠微流控芯片成功地从全血中提取出纯度较高的基因组DNA,提取效率约35%,提取液的凝胶电泳条带与商品化试剂盒提取的基因组DNA一致,提取液可用于进一步的PCR反应。国家星火计划项目(No.2015GA721002);;黄土与第四纪地质国家重点实验室开放基金(No.SKLLQG1607);;福建省中青年教师科技计划项目(No.JAT170817)资助~

Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR

An agarose droplet method was developed for highly parallel and efficient single molecule emulsion PCR. The method capitalizes on the unique thermoresponsive sol-gel switching property of agarose for highly efficient DNA amplification and amplicon trapping. Uniform agarose solution droplets generated via a microfluidic chip serve as robust and inert nanolitre PCR reactors for single copy DNA molecule amplification. After PCR, agarose droplets are gelated to form agarose beads, trapping all amplicons in each reactor to maintain the monoclonality of each droplet. This method does not require cocapsulation of primer labeled microbeads, allows high throughput generation of uniform droplets and enables high PCR efficiency, making it a promising platform for many single copy genetic studies.National Scientific Foundation of China [20805038, 21075104, 20620130427]; National Basic Research Program of China [2007CB935603, 2010CB732402

A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection

National Basic Research Program of China [2010CB732402, 2013CB933703]; National Science Foundation of China [21205100, 21275122, 21075104]; National Instrumentation Program [2011YQ03012412]; Fundamental Research Funds for the Central Universities [2012121025]; Natural Science Foundation of Fujian Province for Distinguished Young Scholars [2010J06004]A T7 exonuclease-assisted cyclic enzymatic amplification method (CEAM) was combined with rolling circle amplification (RCA) to develop a RCA-CEAM dual amplification method for ultrasensitive detection of microRNA with excellent selectivity

Developments and Applications of Paper-based Microfluidics

纸芯片微流控技术是一种新型微流控技术。相比于以玻璃、石英、高聚物等为基底的传统微流控芯片,纸芯片具有成本低、易操作、可携带、耗样量小等优点。该文介绍了纸芯片的发展及常用的制作方法,并举例说明了光度法、荧光法、化学发光及电化学发光法和电化学法在纸芯片检测中的应用;归纳了纸芯片技术在临床诊断、环境监控以及食品安全分析等方面的应用;最后对纸芯片微流控的应用前景进行了展望。Paper-based microfluidics stand out as a new class of microfluidic technology,and present distinguishing features such as low cost,ease of use,portability,and low reagent consumption compared with the conventional microfluidic devices.In this paper,the development of μPADs was first introduced,and the common fabrication techniques were presented.Then the methods for quantitative analysis on μPADs were summarized including colorimetry,fluorescence,chemiluminescence,electrochemiluminescence and electrochemistry with their applications in clinical diagnostics,environmental monitoring as well as food quality control.Finally,the potential and future outlooks ofμPADs were discussed.国家重点基础研究发展计划项目(2010CB732402;2013CB933703); 国家自然科学基金项目(91313302;21205100;21275122); 国家杰出青年科学基金项目(21325522

Backbone-modified molecular beacons for highly sensitive and selective detection of microRNAs based on duplex specific nuclease signal amplification

National Basic Research Program of China [2010CB732402, 2013CB933703]; National Science Foundation of China [21205100, 21275122]; National Instrumentation Program [2011YQ03012412]; Natural Science Foundation of Fujian Province for Distinguished Young Scholars [2010J06004]Based on backbone-modified molecular beacons and duplex-specific nuclease, we have developed a target recycling amplification method for highly sensitive and selective miRNA detection. The combination of a low fluorescence background of 2-OMe-RNA modified MB and nuclease-assisted signal amplification leads to ultrahigh assay sensitivity, and the powerful discriminating ability of MB enables the differentiation of highly similar miRNAs with one-base difference, both of which are of great significance to miRNA detection