山东电网新能源消纳关键措施分析及综合评估

在“双碳”目标下，山东省将进一步加快能源转型步伐，建设新型电力系统。然而，在山东省新能源快速发展的同时，新能源消纳问题日益凸显。以山东电网为分析对象，开展新能源消纳关键措施研究。分析了山东新能源消纳现状，总结了新能源消纳问题的机理，在此基础上归纳影响新能源消纳的关键因素。针对这些关键因素，系统性地提出了促进新能源消纳的各项措施。以山东电网2025年规划数据为基础场景，利用时序生产模拟方法对这些措施进行量化分析，为合理制定新能源消纳措施提供决策支撑

红色荧光蛋白在毕赤酵母中的高效表达

报道了红色荧光蛋白(DsRFP)在毕赤酵母中的高水平表达.利用PCR技术从YEpFLAG- 1 -DsRFP扩增出DsRFP编码序列,克隆到毕赤酵母胞内表达载体pPIC3. 5K构建重组表达载体pPIC3. 5K- DsRFP,电击转化进毕赤酵母,G418 RDB平板双重筛选后获得重组转化子,经MM/MD平板培养与PCR鉴定,重组子全部为HIS+MUT+表型.重组菌株诱导表达48h后获得了高效表达,摇瓶中表达水平达到细胞内总蛋白的12%,表达量达到1. 8g/L.经硫酸铵分级沉淀,DEAE- 5PW阴离子交换柱层析与S- HyperD阳离子交换柱层析后获得电泳纯蛋白.说明我们建立的毕赤酵母表达应用平台具有高效表达外源蛋白的能力

闽南地区TT病毒的变异及经输血传播的初步证据

TT virus(TTV)DNA was tested by nested-PCR from sera of hepatitis patients and volunteer blood donors in Minnan area. The amplified segment was a 189 base pair region in TTV ORF2. A total of six sequences were obtained from three non-A to G hepatits patients and two from volunteer blood donors. The sequences were found to be with 82.9% to 99.3% homology to TTV Japanese strain and Chinese strain. The divergence of sequence in these six segments varied from 0.7% to 17.1%, which indicated that the TTV had been existing for a long time in this area. In the serum of a non-A to G hepatitis patient who was negative for TTV DNA in the 14th day of disease course turned to be positive in the 30th day, two TTV sequences were obtained which showed 92.1% nucleotide homology. It indicated that different TTV strains can co exist in the same person. This patient's blood had been transfused ten times between the collection of his TTV negative sample and his positive serum sample. Seven of the blood donors were traced an..

TT病毒与肝炎关系的临床流行病学研究

目的　对闽南地区各种肝炎患者、健康体检者、义务献血员和肝癌患者共480例从临床流行病学角度探讨TT病毒(TTV)的致病性及其与各种肝炎的关系。方法　采用巢式PCR检测血清TTVDNA、ELISA检测血清抗HAVIgM、HBsAg、抗HBcIgM、抗HCVIgG、抗HEVIgG,用EPIINFO60软件进行统计分析。结果　480名研究对象中TTVDNA的总检出率为23.96%。各种肝炎患者的TTV总阳性率为2394%,肝癌患者的TTV阳性率为2069%,而健康者的TTV阳性率为2484%,义务献血员的阳性率为3000%,均未见明显差别。从临床类型看,急性肝炎、慢性肝炎和重症肝炎的TTV阳性率都在25%左右;从病原类型看,非甲～戊型肝炎的TTV阳性率为2619%,并未见与相应健康者的2523%阳性率的差别;除HCV由于感染率太低而无法分析外,HAV、HBV、HEV阳性肝炎患者间TTV的阳性率分别为2000%、2314%、2179%,未见TTV与这些已知肝炎病毒的明显相关。对一个时期内的全部135例住院肝炎患者及153名健康者进行肝炎病原分析,HAV、HBV、HEV在肝炎患者中的阳性率都要明显高于健康人(P=00142),而TTV在肝炎患者中的阳性率与健康人没有明显差别(P=06021);对病毒的单独致病性进行分析,HAV、HBV、HEV在非重叠感染的肝炎患者中的阳性率都要明显高于健康人(P=00037),而TTV在非甲～戊型肝炎患者中的

从中国非甲-庚型肝炎病人中克隆到TT病毒样DNA序列

从中国非甲－庚型肝炎病人中克隆到ＴＴ病毒样ＤＮＡ序列张军１杨海杰１苏智军２张奕返２林长青１黄鹤１郭庆１王颖１曾定１夏宁邵１（１厦门大学肿瘤细胞工程国家专业实验室厦门３６１００５２福建省泉州市第一医院泉州３６２０００）肝炎是严重危害我国人民身体健康的疾..

Content distribution characters and potential ecological risk assessment of heavy metals in surface sediments of Meizhou Bay

根据2012年福建湄洲湾海域表层沉积物样品测定的7种重金属(Cr、CO、nI、Cu、zn、AS、Pb)含量数据,研究重金属在沉积物中的含量和空间分布特征,然后运用主成分分析法分析各重金属的主要来源,最后采用潜在生态危害指数法评价其污染程度和潜在生态危害.结果表明,相对第一类海洋沉积物标准,Cu、zn、AS和Pb含量均未超标,Cr超标21.2%.大部分重金属含量平面分布总体呈现东北往西南递减的趋势,体现陆源输入的影响,AS、zn及Pb在7号站位、Cu在8号站位出现高值区可能更多地联系于研究区域本身的背景特征.Cr、CO、nI和zn含量之间相关性显著,且CO、AS和zn含量也两两表现出显著正相关.主成分分析表明岩石的自然风化和侵蚀是Cr、CO、nI、Cu、zn和AS元素在研究区域表层沉积物的主要来源,Pb可能更多来源于海上交通航运和海水养殖.研究区域综合潜在生态危害为中等生态危害程度.Based on data of heavy metals in surface sediments of Meizhou Bay in 2012,this paper first analyzed the content,characteristics and spatial distribution of heavy metals(Cr,Co,Ni,Cu,Zn,As,Pb).The sources of heavy metals were studied by principle component analysis(PCA).Finally,their potential ecological risk was assessed through the potential ecological risk index.The results showed that the average content of Cu,Zn,As and Pb were qualified by the first class of marine sediment quality,but surpassed by 21.2% for Cr.The content of most heavy metals decreased from the northeast to the southwest of study area,which was obviously caused by the land source input,but sediment background maybe the main source of As,Zn and Pb at station 7,and of Cu at station8.Strong correlations existed among Cr,Co,Ni and Zn.Co,As and Zn also had significant positive correlations in every two elements.Principle component and background valves analysis showed that rock weathering was the dom-inant source of Cr,Co,Ni,Cu,Zn and As,while Pb was mainly related to the marine traffic and mariculture.The comprehensive potential ecological risk was evaluated as median in the studied areas.国家海洋公益性科研专项资助项目(201005012

Application of principal component analysis to sediment sample classincation

本文利用化学模式识别技术的主成分分析（PCA）法对厦门西港及香港维多利亚港的18个沉积物样品进行分类研究。所涉及的变量（环境指标）包括重金属Cu、Pb、zn、Cd，有机污染物ddT、PAH、PCb、HCH，及碱性磷酸酶活性（APA）。正确的分类基于变量的适当选择，即选择代表来源不同的样品之典型特性的变量组合方式。结果表明，运用PCA技术可获知大量样品的统计特征，对分辨来源不同的样品颇具潜力。Pattern recognition technique was was applied to classify the sediment samples fromXiamen Western Harbour and Victoria Harbour of HOng Kong.In this study the technique ofprincipal component analysis (PCA) of pattern recognition to distinguish the sediment sampleswas adopted.The variables included the heavey metals Cu.Pb.Zn.Cd, the organic pollutantsDDT.PAH .PCB .HCH, and alkaline phosphatase activity.The optimized classification is based onwell selecting the variables.The variables selected, on the other hand, most often represert thetypical characteristics of the sediment samples collected from the different harbours.The resultsshow that PCA technique may offer the potential strategy in the distinguising classes of the environmental samples rome the different origins.国家自然科学基金!No2947727

Monitoring and Preservation of Coastal Bathing Water Quality of Xiamen in Swimming Season *

为调查厦门市海水浴场泳季水质状况，并为监测管理工作提出对策建议，根据1997-5~10月对厦门市3处有代表性的沙滩海水浴场主要水质指标，如粪大肠菌群（fC）、dO、SS及石油类等的监测分析，确认fC为主要污染因子，因其含量单次测值间差异十分显著.采用数理统计方法研究其含量的时空分布及变化.研究结果表明，3浴场水质状况基本符合本市潮间带功能区划的相应要求.建议加强对沙滩浴场的综合整治和陆源污水排海方式的管理.This study is based on the results of water quality monitoring of 3 representative coastal bathing beaches in Xiamen,performed over the time May to November,1997.Analytical results of the main water quality indicators,including fecal coliform bacteria(FC),DO,SS and petroleum,supported that water quality of the concerned bathing beaches was fundamentally conforming to the requirements of functional zonation of inter tidal area in xiamen.The key water pollution factor was identified as FC,which had significant perturbations of its population among individual determinations,ranging from 20 to 2 4×10 5 ind·L -1 .Statistical method was employed to describe the temporal and spacial distribution of FC populations and their variability in the 3 beaches,with averages of 1 1×10 3ind·L -1 ,1 4×10 4ind·L -1 and 1 9×10 4ind·L -1 ,respectively.Advice on monitoring and management of water quality was raised as integrated treatment of the beaches,and strengthening the control of sewage discharging.全球环境基

新疆叶尔羌河冰川湖突发洪水对气候变化的响应

利用1961—2008年的气象-水文资料,探讨了气候变化与叶尔羌河流域冰川湖突发洪水的关系.采用非参数Wilcoxon统计检验和Kendall的τ关联检验分析温度、降水变化与洪峰流量量变化的关联性和一致性;用Mann-Kendall法对气温、降水和洪峰流量>4000 m3.s-1突发性洪水的0℃层高度进行突变检验和趋势分析.结果表明:流域气温在1995年发生突变,且对冰川湖突发洪水发生起主导作用;降水突变不明显,对冰川湖突发洪水发生只起促进作用.自1880年以来,冰川湖突发洪水发生频率增加,与流域气温变化一致.1994年以来气温呈直线上升,洪水频率也呈显著增加趋势;冰川湖突发洪水发生频率与降水变化关系不明显;突发性洪水发生与其前8 d的0℃层高度显著上升密切相关