老人福祉施設における看護職の仕事のやりがいと困難感の構造

本研究の目的は、新潟県内の老人福祉施設で働く看護職に質問紙調査を実施し、仕事へのやりがいと困難感の構造を明らかにすることである。両者の変数の因果関係や相互関係について解析した結果、仕事へのやりがいは「看護アセスメント・処置」がベースとなって、「生活援助等」、「連絡調整等」、「他者からの評価」に影響を及ぼしていた（p=0.030）。「看護職としての専門性が発揮できることが要因ではないかと考えられた。仕事への困難感は、「看護アセスメント・処置」が「生活援助等」と「連絡調整等」に、さらに「連絡調整等」が「他職種との連携」に影響を及ぼしていた（p=0.041）。やりがいの共分散構造モデルと極めて類似した構造となっており、やりがいを感じると同時に困難感も抱いていることを示していた。また、看護職の経験年数30年未満と30年以上での困難感の平均値差は30年未満が「他職種との連携」(p=0.041)と「研修」(p=0.027)において、現施設での経験年数10年未満と10年以上の困難感の平均値差は10年以上が「連携調整等」において、有意に高かった（p=0.013）。さらに、やりがいの有無による困難感の平均値差は、やりがい無し群は、「他職種との連携」において困難感が有意に高いことが明らかになった（p=0.039）。看護職から他職種に歩み寄る姿勢を示し、助け合える人間関係を築くことが連携における課題であり、この改善が老人福祉施設における看護職の困難感の低減、ひいてはやりがいにつながると考えられた。」　The purpose of this study is to reveal the structure of the satisfactions and difficulties that nursing staff members feel in their job by conducting a questionnaire survey with those working at a welfare facility for the aged in Niigata Prefecture.The analyses of causal associations and mutual relationships between the variables of the both feelings found that their feeling of satisfaction based on “Nursing Assessment/Treatment” exercised influences on “Livelihood Support, etc.”, “Coordination, etc.” and “Evaluation by Others”(p=0.030). The satisfaction seems to derive from the moments when nurses are allowed to exercise their professional skills.　Meanwhile, as for their feeling of difficulty“, Nursing Assessment/Treatment” made an impact on“ Livelihood Support, etc.” and“ Coordination, etc.” and, further“, Coordination, etc.” had an influence ove“r Cooperation with Other Professionals”(p=0.041). The covariance structure of sense of difficulty is extremely similar to that of the sense of satisfaction, indicating how nurses find their profession satisfying and at the same time difficult. From the comparison of averages, it was identified that nurses with less than 30 years of professional experience found “Cooperation with other professionals”(p=0.041) and“ training” (p=0.027) significantly more difficult than found among nurses with 30 years or longer experience.Compared to nurses with less than ten years of experience, nurses with ten years or longer experience found “coordination” significantly more difficult (p=0.013). Further, with respect to the difference in averaged values for the sense of difficulty between those with the sense of satisfaction and those without it, a significant difference was found in the latter group in terms of“ Cooperation with Other Professionals”, demonstrating that their sense of difficulty was significantly higher (p=0.039). Through the study, establishment of a mutually supporting relationship, initiated by nurses approaching other professionals, is identified as the key issue in a collaboration, and an improved relationship is suggested as the factor contributing to reduced sense of difficulty and increased sense of satisfaction among nurses working at elderly welfare facility

Microbial community and moss gemmae aggregation on disappearing tropical glacier in Uganda

第4回極域科学シンポジウム個別セッション：[OB] 生物圏11月13日（水）13:00-14:15　国立国語研究所 ２階ラウン

セルロース担体セルロファインに対するセルロース結合部位を付加した融合タンパク質の吸着

A fusion protein gene of endo‒beta‒glucanase (EG) attached cellulose binding module (CBM) was constructed. The end‒beta‒glucanase gene was cloned from fungus Robillarda sp. strain NFRI1090 and the CBM gene was cloned from fungus Trichoderma viride. The fusion protein gene was transformed to Pichia pastoris gene expression system and the fusion proteins were produced as recombinant protein. The fusion protein, EG attached CBM, adsorbed on Cellulofine CG‒700 m column in low salt concentration buffer and easily eluted by high salt concentration buffer. The fusion protein method with CBM is useful for separation of a recombinant protein from crude protein mixture.糸状菌 Robillarda sp. NFRI1090 株由来エンド‒β‒グルカナーゼ (EGI) にTrichoderma viride 由来セロビオヒドロラーゼ I のセルロース結合モジュール (CBM) を付加した組み換え融合タンパク質遺伝子を作製し, Pichia pastoris の分泌発現系に導入することよって生産した. 得られた組み換え融合タンパク質のセルロース担体カラムに対する吸着, 脱着を行い, 融合タンパク質 (EGI‒linker‒CBM) が Cellulofine CG‒700 m カラムに対して低塩濃度にて吸着し, 高塩濃度条件にて容易に溶出回収することが可能であることを明らかにした. CBM を付加した組み換え融合タンパク質を Cellulofine によって吸着, 溶出することは, 組み換えタンパク質の分離精製において有用である

小麦による食物性アナフィラキシーに急性膵炎を合併した小児例

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