独活寄生汤水提物对退变大鼠椎间盘软骨细胞功能的影响

目的探究独活寄生汤水提物对经IL-1β诱导退变的大鼠椎间盘软骨细胞中Wnt4、GSK-3β、β-catenin、DKK-1的影响。方法采用机械-酶消化法分离大鼠椎间盘软骨组织,进行软骨细胞体外培养、镜下观察与鉴定,分为正常组、模型组(经10 ng/m L浓度IL-1β造模)、实验1组(独活寄生汤水提物组200μg/m L干预24 h)、实验2组(独活寄生汤水提物组200μg/m L干预48 h)。观察4组大鼠椎间盘软骨细胞中Wnt4、GSK-3β、β-catenin、DKK-1 m RNA与蛋白的表达及上清液中Sox 9表达。结果 (1)第2代大鼠椎间盘软骨细胞增殖速度快,呈现多边形,胞核清晰,且含有1~2个核仁,融合后出现\"铺路石\"状,经Ⅱ型胶原法染色后,阳性对照组胞浆区域浸染为棕黄色;(2)与正常组比较,模型组中软骨细胞Wnt 4、GSK-3β、β-catenin m RNA与蛋白表达及上清液Sox 9含量明显提高(P<0.05),DKK-1 m RNA与蛋白表达明显降低(P<0.05);与模型组比较,实验1组、实验2组中软骨细胞Wnt 4、GSK-3β、β-catenin m RNA与蛋白表达及上清液Sox 9含量明显降低(P<0.05),DKK-1m RNA与蛋白表达明显提高(P<0.05),以实验2组变化最为显著(P<0.05)。结论独活寄生汤水提物组可调控退变椎间盘软骨细胞的功能,下调Wnt 4、GSK-3β、β-catenin和上调DKK-1 m RNA与蛋白表达,进而延缓大鼠椎间盘软骨细胞的退变。福建省自然科学基金项目(2017J01224);;福建中医药大学校管课题资助(X2015034

独活寄生汤调控对大鼠椎间盘软骨细胞Wnt/β-catenin信号通路的影响

目的:从Wnt/β-catenin信号通路探讨独活寄生汤水提物对大鼠椎间盘退变软骨细胞功能的影响。方法:（1）用水提加热回流法制备独活寄生汤水提物成分;（2）选取4周龄健康雄性SD大鼠30只,采用机械-酶消化法分离大鼠椎间盘软骨组织,建立软骨细胞体外培养体系并进行鉴定;（3）RT-PCR、We stern blot法分别检测经DKK-1抑制剂干预及（或）经白细胞介素-1β诱导的椎间盘软骨细胞Wnt4、GSK-3β、β-catenin mRNA与蛋白含量的表达。结果:（1）椎间盘软骨细胞经Ⅱ型胶原法染色后,阳性对照组胞浆区域浸染为棕黄色;（2）RT-PCR、Western blot检测结果示,与正常组比较,模型组Wnt4、GSK-3β、β-catenin mRNA与蛋白含量表达显著升高（P<0.01）;与模型组比较,独活寄生汤水提物组(100,200,400μg·mL-1)的Wnt4、GSK-3β、β-catenin mRNA与蛋白含量表达显著降低（P<0.01或P<0.05）,其中以200μg·mL-1组的表达量最低（P<0.01）。结论:独活寄生汤水提物组可通过调控Wnt/β-catenin信号通路,下调大鼠椎间盘退变关节软骨中Wnt4、GSK-3β、β-catenin mRNA与蛋白含量表达,从而延缓椎间盘软骨细胞退变。福建省自然科学基金(2017J01224);;福建中医药大学校管课题(X2015034

Preventive effect and mechanisms of 3,3-diindolylmethane on oxidative stress induced by hydrogen peroxide in HaCaT cells

目的:探究3,3'-二吲哚甲烷(dIM)对过氧化氢(H_2O_2)诱导人角质形成细胞(HACAT)氧化应激作用的预防效应及可能机制。方法:体外培养HACAT细胞,用H_2O_2建立氧化应激模型。采用CCk-8法检测不同浓度(1~20μMOl/l)dIM对HACAT细胞生长的抑制作用;流式细胞术检测dIM作用前后细胞内活性氧自由基(rOS)含量的变化;WESTErn blOT检测不同浓度dIM(0、5、10μMOl/l)对HACAT氧化应激相关蛋白核因子(nf-κb)和丝裂原活化蛋白激酶(MAPkS)磷酸化表达水平的影响。结果:成功建立了HACAT氧化应激模型。CCk-8法研究结果显示1-10μMOl/l dIM对HACAT细胞无明显毒性作用(P>0.05);流式细胞术检测结果表明10μMOl/l dIM预处理可有效预防由H_2O_2诱导的HACAT内rOS产生(P0.05).Flow cytometry results indicated that pretreatment with DIM(10 μmol/L) could inhibit the level of intracellular ROS(P<0.05).With increasing concentration of DIM,the levels of p-p38-MAPK,p-JNK and p-NF-κB were significantly depressed.CONCLUSION:DIM could protect HaCaT cells from H_2O_2-induced oxidative stress via suppressing production of ROS levels and down-regulating the expression of NF-κB and members of MAPKs.DIM might be used as an effective drug to treat or reduce oxidative stressmediated injury in skin cells.国家自然科学基金青年基金(81101562); 广东省科技计划项目(2012B060300005); 广东省自然科学基金(S2012010009633

Hematite concave nanocubes and their superior catalytic activity for low temperature CO oxidation

Hematite (α-Fe2O3) concave nanocubes bound by high-index {1344} and {1238} facets were synthesized and their catalytic activity for CO oxidation were also investigated. ? 2014 the Partner Organisations

Development of Computer Band One Testing System

给出了一个基于 Windows98/NT平台和 SQL SERVER的计算机一级考试系统的详细设计及实现方法 .系统利用 VB特点 ,设计了一个新的教学辅助考试系统 ,由六大模块组成 .包括考生管理模块、考试模块、评卷模块、成绩查询、试题录入模块和考卷查阅模块 .能对公共基础课进行上机测试 .是一种全新的、可操作的、通用的教学考试系统 .A concrete design and implementation method of Computer Band One Testing System based on Windows 98/NT operating system and SQL Server are proposed. The testing system is developed with Visual Basic, combining design idea and traditional science knowledge, which has six functional modules, including examinee-managing module, testing module, paper-correcting module, score-searching module, exam-question-importing module and paper-consulting module. It can undertake Computer-Based Test for theoretical and experiment of public fundamental lessons. It is a new-styled, operative, and general teaching testing system

Research of Improvement and Optimization on Association Rules Mining Algorithm

首先对Apriori算法过程本身进行了详细的研究,给出了三种改进措施,各种改进措施在特定的应用场合有着明显的优点,均能有效减少存储候选集所占用的空间或算法过程占用的时间,之后着重对强关联规则的生成算法进行了详细讨论,给出了优化算法,实例表明该算法能有效减少相关计算量,比已有算法运算效率明显提高.The most classical algorithm Apriori was studied and then separated into six steps:linking→cutting→generating C_k→scanning and counting→comparing→generating L_k. After that three improved measures were provided:1) judge if c∈C_k,2) scan while selecting,3) scan directly while bypassing cutting.Every measure has obvious advantages under special situation,and can effectively reduce the space occupied by candidate itemset and the time occupied by steps.Then a deep research was made on association rules generating process in detail,and a developed algorithm was given.The developed algorithm can decrease related computation quantity in large scale and improve the efficiency of the algorithm.福建省自然科学基金(A0310008);; 福建省高新技术研究开放计划重点项目(2003H043)资

生物基聚酯改性聚乳酸树脂及其制备方法与加工成型方法

本发明公开了一种生物基聚酯改性聚乳酸树脂及其制备方法与加工成型方法。所述聚乳酸改性树脂包括：聚乳酸67～95wt％，聚呋喃二甲酸丁二醇酯4～30wt％，抗氧剂0.5～1wt％，以及助剂0.5～2wt％。所述制备方法包括：将聚乳酸、聚呋喃二甲酸丁二醇酯充分干燥后，再与抗氧剂及助剂密炼共混而获得目标产品。本发明的改性聚乳酸树脂是一种可再生、环保的树脂，其拉伸强度≥60MPa，断裂伸长率在180～240％，当断裂伸长率为240％时拉伸模量仅下降25％、拉伸强度仅下降16％，且无需添加珠光粉，表面就具有珠光色反光，可用于制备薄膜、板材、片材、发泡和注塑成型塑料制品等，具有广阔工业应用前景

Analysis on a Frame Alignment Algorithm for Video Quality Assessment

在全参考或者部分参考的视频质量评价算法中,由于视频序列在传输过程中总会存在丢帧、跳帧、停滞等现象,因此在视频质量评价之前进行的帧对齐处理是非常重要的步骤.提出了一种新的帧对齐算法,该方法在国际电信联盟(ITu)的ITu-T J.244和J.247的基础上,对源视频和劣化视频序列进行预处理和相关特征参数的提取,并采用类似滑动窗口的原理对二者进行相似度的运算,根据运算结果可判断是否达到帧对齐.结果表明,该方法可以明显甄别出视频序列相应帧的对齐情况,算法简单、实时性较好,具有一定的实用价值.In full or reduced reference video quality assessment algorithms,frame alignment is an important procedure before any video quality assessment can be performed due to the frame-loss,frame-jump,or frame-stuck during video sequence transmission.In this paper,a novel frame alignment algorithm is proposed,which is based on ITU J.244 and ITU J.247.This algorithm pre-processes the source video and degraded video sequence to extract the characteristic parameters.Then a sliding-window type algorithm is applied to calculate the similarity of the two sequences.The calculation result is used to judge the frame alignment.The algorithm proposed in this paper is simple and can be applied in real-time video process.广州京信基金项目(XDHT2010156A

脉搏波的频谱分析

从血管壁的运动来判别疾病,在医学上已有很长时期的历史了.早在“黄帝内经”中就有专门的论述,并一直延续到今天的中医诊断中.1958年陈可冀等人用压电晶体传感器