Intervention effect of salidroside on liver fat synthesis and oxidation of non-alcoholic fatty liver in rats induced by high-fat diet

目的:基于肝脏脂肪合成和氧化环节,探讨红景天苷防治非酒精性脂肪肝的作用机制。方法:采用单纯高脂饮食14周诱导的大鼠非酒精性脂肪肝模型。在造模第9; 周起,随机分为模型组、红景天苷组和罗格列酮对照组,灌胃给药6周。观察肝组织病理变化;肝组织甘油三酯(TG)、游离脂肪酸(FFA)含量的变化;肝组; 织乙酰辅酶A羧化酶(ACCase)、丙二酰辅酶A (Mallonyl CoA)、脂肪酸合成酶(FAS)、肉毒碱棕榈酰转移酶-1; (CPT-1)含量的变化;肝组织ACCaseCPT-1; mRNA水平的变化。结果:模型组肝组织出现显著的肝细胞脂肪变性及空泡样变,肝组织TG、FFA、ACCase、FAS、 Malonyl; CoA含量和ACCase; mRNA水平较正常组均显著升高(P<0.01),CPT-1含量和mRNA水平较正常组显著降低(P<0.01)。红景天苷组的上述病理改变显著减轻,; 肝组织TG、FFA、ACCase、 Malonyl CoA、FAS含量和ACCase mRNA水平显著低于模型组(P<0.01) ,; CPT-1含量和; mRNA水平显著高于模型组(P<0.01)。结论:红景天苷能抑制肝脏脂肪合成,促进脂肪酸氧化,这可能是其防治非酒精性脂肪肝的重要机制。Objective: To explore the mechanism of salidroside on non-alcoholic; fatty liver disease based on liver fat synthesis and oxidation. Methods:; Non-alcoholic fatty liver disease model was induced by high-fat diet for; 14 weeks. From the ninth week, the rats were randomly divided into model; group, salidroside group and rosiglitazone group, and were given a; gavage for six weeks. The observing items including: pathological; changes of liver tissue (HE staining); changes of contents of; triglycerides (TG) and free fatty acid (FFA) in liver tissue; changes of; contents of acetyl-Coacarboxylase (ACCase), malonyl CoA, fatty acid; synthase(FAS) and carnitine palmitoyl transterase-l(CPT-l); changes of; mRNA levels of ACCase and CPT-1 in liver tissue. Results: Hepatocellular; steatosis and vacuolar degeneration were observed in the liver tissue of; the model group. The contents of TG, FFA, ACCase, Malonyl CoA, FAS and; mRNA level of ACCase in model group were significantly higher than those; of the normal group (P<0.01). The content and mRNA level of CPT-1 were; significantly lower than those of normal group (P<0.01). Hepatic; pathological changes in salidroside group were significantly reduced.; The contents of TG, FFA, ACCase, Malonyl CoA, FAS and mRNA level of; ACCase were significantly lower than those of model group (P<0.01). The; content and mRNA level of CPT-1 were significantly higher than those of; model group (P<0.01). Conclusion: Salidroside can inhibit liver fat; synthesis and promote the oxidation of fatty acid, which may be an; important mechanism of salidroside for prevention and treatment of; non-alcoholic fatty liver disease.国家自然科学基金项目; 浙江省自然科学基金项

Intervention effect of Chinese herb components HJJB Compound on PP1-DNA-PK-USF1 signaling pathway of non-alcoholic fatty liver rats

目的:基于蛋白磷酸酶(PP1)-DNA依赖的蛋白激酶(DNA-PK)-上游刺激因子1(USF1)信号通路,探讨中药组分HJJB方防治非酒精性脂肪; 肝(NAFLD)的作用机制。方法:采用高脂饮食诱导的大鼠NAFLD模型。设模型组、HJJB方组和罗格列酮组,分别灌胃给药6周。HE染色观察肝组织; 病理变化;测定肝组织甘油三酯(TG)、游离脂肪酸(FFA)含量的变化;肝组织PP1、DNA-PK、USF1; mRNA水平和蛋白含量的变化。结果:模型组肝组织出现显著的肝细胞脂肪变性及空泡样变,肝组织TG、FFA含量较正常组显著升高(P<0.01),肝组; 织PP1、DNA-PK、USF1; mRNA水平和蛋白含量较正常组均明显升高(P<0.01)。HJJB方组的上述病理改变明显减轻,肝组织TG、FFA含量较模型组显著降低(P<0.0; 1),肝组织PP1、DNA-PK、USF1; mRNA水平和蛋白含量较模型组显著降低(P<0.01)。结论:HJJB方能显著降低脂肪肝大鼠肝组织PP1; mRNA水平和蛋白含量,进而抑制其下游信号通路,这可能是其防治NAFLD的重要机制。Objective: Toexplore the mechanism of Chinese herb component HJJB; Compound on non-alcoholic fatty liver disease based on PP1-DNA-PK-USF1; signaling pathway. Methods: SD male rats were induced by high-fat diet; for nonalcoholic fatty liver disease model. The rats were randomly; divided into model group, HJJB group and rosiglitazone group, and were; given intragastric administration for six weeks. The observing items; including: liver pathology(HE staining); hepatic triglyceride(TG) and; free fatty acid(FFA) content; mRNA and protein content changes of; hepatic protein phosphatase 1(PP1), DNA-dependent protein kinase(DNA-PK); and upstream stimulating factor 1(USF1). Results: Significant hepatocyte; fatty degeneration and vesicle changes were observed in liver tissue of; model group. The hepatic TG and FFA contents of model group were; significantly higher than those of normal group(P<0.01), and mRNA levels; and protein contents of liver tissue PP1, DNAPK, USF1 in model group; were significantly higher than those in normal group(P<0.01). Hepatic; pathological changes in HJJB Compound group were meliorated, liver; tissue TG and FFA contents of HJJB Compound group were significantly; lower than those of model group(P<0.01), and mRNA levels and protein; contents of liver tissue PP1, DNA-PK, USF1 mRNA of HJJB Compound group; were significantly lower than those of model group(P<0.01). Conclusion:; HJJB Compound can significantly decrease mRNA level and protein content; of PP1 in the liver tissue of fatty liver rats, and then inhibit the; downstream signaling pathway of PP1, which may be an important mechanism; of HJJB Compound for prevention and treatment of non-alcoholic fatty; liver disease.国家自然科学基金项目; 浙江省自然科学基金项目; 浙江省中医药科技计划项

石灰石粉与不同矿物掺合料复掺对高强自密实混凝土性能的影响

基于混凝土的自密实性能和强度试验结果,研究了石灰石粉分别与粉煤灰、矿粉和硅灰3种不同矿物掺合料复掺组合对高强自密实混凝土性能的影响。结果表明:在合适的掺量范围和复配合比例条件下,将低活性的石灰石粉与高活性的硅灰复掺有很好的互补效应,对混凝土的自密实性能和强度产生了积极影响;石灰石粉与矿粉复掺不仅可以改善混凝土的自密实性能,同时也能满足高强要求。石灰石粉与硅灰、石灰石粉与矿粉复掺组合是制备高强自密实混凝土的有效技术手段。石灰石粉与粉煤灰复掺虽然显著提高了混凝土的自密实性能,但是不能满足高强要求,只能制备中、低强度自密实混凝土

Effects of Pb~(2+) stress on nitrogen metabolism and membrane lipid peroxidation in Aegiceras conrniculatum seedlings

研究了Pb2+对桐花树幼苗氮代谢关键酶活性及膜脂过氧化的影响。结果表明:0.1~2.0 mmol.L-1Pb2+胁迫使营养液中培养的桐花树幼苗根、茎、叶的NADH-GOGAT活性降低,丙二醛含量增加,使根和茎的GS活性降低,而叶的GS在0.1~1.0 mmol.L-1Pb2+胁迫下活性增加高于对照,但受2.0 mmol.L-1较高浓度Pb2+抑制。无论是否有Pb2+胁迫,叶的GS活性都高于茎和根,而根的NADH-GOGAT活性始终高于茎和叶。根的GS和NADH-GOGAT活性受Pb2+胁迫抑制的程度明显大于茎、叶,Pb2+对根的初级氮代谢影响最大。MDA含量与GS和NADH-GOGAT活性呈负相关关系。The effect of Pb2+ stress on nitrogen metabolism and membrane lipid peroxidation in Aegiceras corniculatum seedlings was investigated.The activities of NADH-GOGAT and GS,soluble protein,and MDA content in the roots,the stems and the leaves of A.corniculatum seedlings,which were cultivated for 30 days in nutrient solution containing Pb2+,were analyzed.The results showed that the NADH-GOGAT activities in A.corniculatum seedling roots,stems,and leaves decreased under Pb2+ stress,while the contents of soluble proteins and MDA increased.The GS activities in the roots and stems decreased due to Pb2+ treatments with concentrations ranging from 0.1 to 2 mmol·L-1.GS activities in the leaves increased and was higher than that of the control.However,the activity was inhibited by Pb2+ at higher concentration of 2 mmol·L-1.Whether Pb2+ was added to the nutrient solution or not,the GS activity in the leaves was higher than those in the roots and stems.The GOGAT activity in the roots was higher than those in the leaves and stems.GS and NADH-GOGAT in roots were more sensitive to Pb2+ stress than those in stems and leaves.Thus,Pb2+ had the most effect on primary nitrogen metabolism in the root.It was also suggested that there was a negative correlation between the MDA content and the NADH-GOGAT or GS activity.福建省青年科技人才创新项目(2004J053);; 福建教育厅资助项目(JA02251);; 泉州市科技局资助项目(2003Z11

学术图书馆学科导航门户资源类型表的设定

在对DC和ROADS以及SOSIG、INFOMINE和CSDL等大型学科导航门户对资源类型设定的调研基础上,提出了区分和设定资源类型的原则,并根据该原则设定了适合于CALIS学科导航门户建设的资源类型表

Exploration of the Practical Training Program for Materials Science and Engineering Students

实践教学是材料专业人才培养的重要环节,实践教学体系是提高实践教学质量的重要保障。厦门大学材料学院紧紧围绕应用型、复合型、创新型人才培养目标,注重学生“五种能力“的培养,改革和创新材料科学与工程专业的实践教学体系,促进了学生的创新能力与实践能力的提高。Laboratory experience is vital to the students for the specialty of Materials Science and Engineering.We have devoted to produce talented scientists,highly qualified engineers and competent administrators through providing excellent education programs,innovative laboratory experiences,up-to-date facilities,leading-edge researches,and important industrial partnerships.The students are offered five levels laboratory and field trainings during their college study to improve their capability on solving practical problems and creating novel techniques and materials

纳米Co_3O_4的制备及其在富氢气氛下CO选择氧化反应中的催化性能

过渡金属氧化物四氧化三钴(Co3O4)在CO氧化反应中展示了较好的低温活性.Co3O4催化剂用于富氢气氛下CO选择氧化反应已引起了人们极大的关注,具有潜在的应用前景.采用液相沉淀-热解氧化方法制备了2种不同形貌的纳米Co3O4.用X射线衍射(XRD)、扫描电子显微镜(SEM)等技术考察了包括沉淀剂种类、老化时间、焙烧温度等合成条件对生成Co3O4形貌、晶粒尺度的影响.制备的Co3O4的外形与沉淀前驱物外形直接相关.焙烧温度越高,Co3O4的颗粒越大.研究了制得的Co3O4在富氢气氛下CO选择氧化反应中的催化性能.对比以上方法制备的Co3O4的催化性能发现,催化剂的粒径和比表面积与催化剂的活性存在关联.使用尿素沉淀法并经250~300℃空气热解氧化制得的Co3O4具有较好的催化活性

Diversity of culturable endophytic fungi of common reed ( Phragmites australis) in coastal wetland

内生真菌在植物生长以及抵御环境胁迫过程中起着非常重要的生态作用.本研究从黄河三角洲滨海湿地1350个芦苇组织切片中分离得到318株内生真菌,通过对rDNA ITS的分型、测序及系统进化分析,研究了该地区芦苇植株根、茎、叶中可培养内生真菌的种类组成及在不同盐度、不同组织中的分布情况.根据序列相似性(以98%为阈值),共获得12个真菌分类操作单元(OTUs).在门级分类水平上,子囊菌门为绝对优势菌群,在各组织和站位中均有分布.芦苇根组织中分离得到的内生真菌OTU数相对较多,叶组织和茎组织中分离到的OTU数相同,且叶中的OTU在根中均存在.潮上区(低盐区)内生真菌OTU数最多,高潮区次之.根中可培养..

New method of variable excitation MFL testing under the condition of small magnetizing apparatus

为解决常规储罐和管道的漏磁检测(Mfl)方法中磁化器体积大、耗能多的缺点,提出了一种小型磁化器条件下的变励磁Mfl检测新方法。首先,分析了小磁化器条件下被测钢板磁化状态随外部励磁强度的变化特性,推导了偏置磁化条件下励磁通变化量与漏磁场变化率的关系模型;其次,根据所建立的关系模型,设计了一种新型变励磁Mfl检测探头,并给出变励磁Mfl法的系统实现方案;再次,结合实现方案,搭建了变励磁Mfl相应的实验平台;最后,在该平台上,开展了一系列的物理实验并对实验结果进行了深入讨论。实验结果表明,所提变励磁Mfl法无需对被测钢板进行局部饱和磁化,适合小型磁化器条件并具有较高的灵敏度。变励磁Mfl法为漏磁检测领域提供了一种新的检测思路。In the conventional method of magnetic flux leakage( MFL) testing,the magnetizer is in big volume and with more energy consumption.To solve the problem,a new method of variable excitation MFL testing under the condition of small magnetizer is proposed.First of all,the state of magnetized steel along with the change of the external excitation's intensity under the condition of small magnetizer is analyzed.Secondly,according to the established model,a new type of testing probe for the variable excitation of MFL is designed and a new implementation scheme is proposed.Furthermore,combining with the implementation scheme,an appropriate testing platform of the variable excitation MFL is set.Finally,a series of physical experiments on the platform are carried out and the results of the experiment were discussed.The experimental results show that the proposed method don't need to make local saturation magnetization of tested steel plate,which is suitable for the condition of small magnetizer with higher sensitivity.The new method provides a new testing idea for MFL testing field.国家自然科学基金(51177141); 中央高校基本科研业务项目(2010121041); 航空基金(2012ZD68003)资助项

嗜人类T淋巴细胞病毒Ⅰ型核心蛋白P24基因的克隆与表达

目的 从感染HTLV-1的中国患者外周血单核细胞（PBMCs）中扩增编码HTLV-1核心蛋白P24的cDNA,并在大肠杆菌中进行表达。方法 提取感染者PBMCs基因组DNA,应用巢式PCR方法扩增出HTLV-1核心蛋白P24的cDNA并测序,与pGEX-20T载体构建重组质粒,在大肠杆菌BL21中表达,采用ELISA和Westernblot分析重组蛋白活性。结果HTLV-1核心蛋白P24基因序列高度保守,构建重组载体后,在大肠杆菌中表达的重组蛋白,经检测具有较强的抗原活性。结论 成功地表达了HTLV-1型病毒的核心蛋白P24基因,为国产诊断试剂和疫苗的研发打下了基础