Synthesis of Eu(BTF)_3phen and Study on Its Property

采用常温搅拌法制备了配合物Eu(bTf)3PHEn(bTf=苯甲酰三氟丙酮,PHEn=邻菲罗啉),改善稀土铕配合物的荧光性能。对配合物的热稳定性和荧光性质进行了测定,结果表明其具有良好的热稳定性,在362 nM激发光激发下,能发射强烈的、来自于Eu3+离子的特征红光,最大发射峰在612 nM,半峰宽大约为8 nM。对邻菲罗啉中性配体进行了两种不同的分子修饰,荧光光谱测试表明,邻菲罗啉配体上引入不同数目的基基团,在一定程度上改善了Eu(bTf)3PHEn配合物的荧光性能。In order to improve the fluorescence properties of europium complex, Eu(BTF)3phen( BTF =Benzoyltrifluoroacetone, phen =o-phenanthroline) was synthesized by the method of stirring method at room temperature.The results showed that the complex has good thermal stability.The complex emitted a strong characteristic red light which was from Eu3 +under the exciting light of 362 nm.The maximum emission peak was at 612 nm.The full width at half maximum( FWHM) was 8 nm.Two different molecular modifications of the phenanthroline neutral ligands were carried out.The fluorescence spectra analysis showed that the fluorescent properties of Eu(BTF)3phen was improved by the introduction of different numbers of neighborhood groups onto the phenanthroline ligands

考虑等级的民航个性化航空路线推荐模型

航空旅客会员等级以及航班舱位等级可以有效区分不同等级的旅客群体,提升航空路线个性化推荐的准确性。在已有的加权矩阵分解模型基础上,引入旅客会员等级和航班舱位等级,建立了考虑等级因素的个性化航空路线推荐模型。通过实际航空运营数据进行实证研究,检验了考虑等级的个性化航空路线推荐模型的有效性。实证结果表明,旅客会员等级和航班舱位等级显著影响航空路线个性化推荐的效果,同时考虑会员等级和舱位等级的航空路线推荐模型的平均召回率,与平均精确率比未考虑等级的航空路线推荐模型分别提高5.6%以及1.1%。研究还发现,考虑等级的航空路线推荐模型在不同等级的旅客数据集上推荐的效果存在差异,不同会员等级和舱位等级的旅客需求不同,舱位等级因素对高端等级旅客显得尤为重要,但对于普通等级的旅客,会员等级的影响因素大于舱位等级。国家自然科学基金资助项目(71672126);国家自然科学基金重点资助项目(71532015

微型铣刀外径视觉测量系统的照明设计

为了满足微型铣刀外径视觉测量系统的照明需求,设计并制作了一种基于柯勒照明原理的平行光源。平行光源由白光lEd、孔径光阑和3组组合透镜组成,使用zEMAX对光路进行了仿真。设计并加工了多级可调金属圆筒机械结构,将光源各部分器件固定组合,光源总长为142 MM。搭建了微型铣刀外径视觉测量系统,并分别使用漫射背光光源和平行背光光源对铣刀进行照明成像。实验结果表明,平行背光照明提高了铣刀测量系统的测量精度,降低了铣刀的测量离差

Preparation and Application of Soluble Human Squamous Cell Carcinoma Antigen Expressed by Escherichia coli

旨在建立基于大肠杆菌表达系统的高效可溶性表达人鳞状上皮细胞癌抗原(SCCAg)方法,获得具有较好活性的重组SCCAg抗原并应用于建立抗原检测方法; 。基于pGEX-6P-l载体和大肠杆菌E. coli; ER2566菌株开展重组SCCAg抗原可溶性表达纯化方法研究,评价纯化抗原活性,筛选特异性单克隆抗体,初步建立并评价SCCAg抗原检测方法。结果; 显示,pGEX-6P-l载体和E coli; ER2566菌株可用于建立较高效的可溶性表达和纯化SCCAg抗原的方法,获得了具有较高纯度和活性的重组SCCAg抗原,筛选获得特异性单克隆抗体并; 初步建立了 SCCAg管式化学发光检测方法。建立了有效的基于大肠杆菌表达系统的可溶性表达和纯化SCCAg的方法。The aims of this study are to establish a method for efficient soluble; expression of human squamous cell carcinoma antigen(SCCAg ) based on; Escherichia coli expression system and obtain the recombinant SCCAg; antigen in fine activity, then apply it in the detection method; establishment of antigen. The study on the method of soluble expression; and purification of recombinant SCCAg antigen was conducted based on; pGEX-6P-l vector and E. coli ER2566 strain. The activity of the purified; antigen was evaluated by Abbott Kit and the specific monoclonal antibody; was screened by indirect ELISA. It was proved that PGEX-6P-1 vector and; E. coli strain ER2566 could be used to establish efficient soluble; expression and purification method for recombinant SCCAg antigen.; Moreover, the recombinant SCCAg antigen was proved to be in high purity; and activity. Thus,the SCCAg detection method of chemical luminous tube; was established with the specific monoclonal antibodies. In conclusion,; an effective method for the expression and purification of SCCAg, which; is based on the E. coli expression system, is established.国家高技术研究发展计划(863计划

圣·约翰草提取物片治疗抑郁障碍专家共识

结合循证医学证据,本文归纳圣·约翰草提取物片治疗抑郁障碍的专家共识

Abundance and production of bacteria and their correlations with environmental factor

以2014年8月南海北部海水样品为研究对象,利用平板计数法和流式细胞仪计数法对南海北部表层和垂直海域可培养细菌和细菌总数分布状况进行研究,对细菌; 生产力进行测定,并结合环境因子进行相关性分析。结果表明:珠江口到南海北部海域,水平方向可培养细菌总数变化范围是3.70*10~2 ~; 1.42*10~3 CFU/mL,细菌总数变化范围是5.12 * 10~5 ~1.61 * 10~6; cells/mL,细菌生产力的变化范围是0.03 ~0.40 mg/m~3/h;垂直方向上可培养细菌变化范围是1.08 *10~3 ~9.00; *10~3 CFU/mL,细菌生产力变化范围是0.01 ~0.08; mg/m~3/h,其中表层海水中的细菌生产力明显高于底层。与环境因子相关性分析表明,水平方向上,影响南海北部表层海水细菌总数和细菌生产力的主要因; 素是温度、盐度、硝酸盐(NO_3-N)、硅酸盐(SiO_3-Si)、亚硝酸盐(NO_2-N)和磷酸盐(PO_4-P)(P<0.05);垂直方向上; ,影响南海北部可培养细菌总数的主要因素是NO_2-N(P<0.05),影响细菌生产力的主要影响因素是温度和盐度(P; <0.05)。可见,南海北部表层海水中细菌总数高于可培养细菌总数2; ~3个数量级,表明该海域表层海水存在大量不可培养细菌;细菌的生命活动在海水表层相较底层更为活跃。Distribution of abundance and production of bacteria and their; correlations with environmental factor were investigated, using plate; count method and flow cytometry, in the surface and vertical waters of; the northern south China sea in August 2014. The cultivable bacterial; abundance ranged from 3.70 * 10~2 CFU/mL to 1.42 * 10~3 CFU/ mL, the; total bacterial abundance ranged from 5.12 * 10~5 cells/mL to 1.61 *; 10~6 cells/mL and the bacterial productions varied from 0.03 mg/m~3/h to; 0.40 mg/m~3/h in horizontal distribution. In vertical distribution, the; cultivable bacterial abundance ranged from 1.08 * 10~3 CFU/mL to 9.00 *; 10~3 CFU/mL and the bacterial productions varied from 0.01 mg/m~3/h to; 0.08 mg/m~3/h, respectively. The correlation analysis results showed; that the environmental factors affecting the abundance of total bacteria; and bacterial productivity included temperature, salinity,; nitrate(NO_3-N),silicate (SiO_3-Si), nitrite (NO_2-N),and phosphate; (PO_4-P) (P<0.05). NO_2-N was the main influencing factor to cultivable; bacteria abundance (P < 0.05), while bacterial productivity was highly; correlated with temperature and salinity in vertical distribution of; northern south China sea (P < 0.05). The total bacterial abundance was 2; ~ 3 orders of magnitude higher than cultivable bacteria, suggesting that; there were many uncultured bacterium in surface water of northern south; China sea and bacterial activities in the surface maybe more active than; in the bottom.全球变化与海气交互作用; 国家重点研发计划全球变化及应对专项; 海洋公益性行业科研专

Diversity of CO_2 fixation gene in the surface waters of northern South China Sea in the Calvin cycle

为揭示南海北部表层海水中参与卡尔文循环的固碳基因多样性及其与环境因子的关系,本研究以卡尔文循环中的关键酶核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubis CO)的Ⅰ、Ⅱ型基因(cbbL、cbbM)作为分子标记,采用Illumina Miseq高通量测序技术对海水中的固碳基因多样性进行分析,并结合多元统计分析的方法,探讨了固碳基因多样性与环境因子的关系.结果显示,在南海北部表层海水中,含cbbL基因固碳基因主要归属于变形菌门(Proteobacteria)、蓝藻门(Cyanobacteria)和厚壁菌门(Firmicutes),其中,优势亚群分别是γ-变形菌亚门(45.3%)、蓝细菌(30.9%)和β-变形杆菌亚门(23.8%);而含cbbM基因的固碳菌群未检测到;近岸的A9站位与其他站位物种组成有一定的差异,异着色菌属(Allochromatium)、硫杆菌属(Thiobacillus)和硫单胞菌属(Thiohalomonas)为其特有菌属.相关性和冗余分析(RDA)结果显示,含cbbL基因的固碳基因丰度与水温、盐度呈显著负相关(p<0.01),与硝酸盐、亚硝酸盐、硅酸盐呈显著正相关(p<0.01).To elucidate the diversity of CO_2 fixation and its relationship with environmental factors in surface water of northern South China Sea,the coding gene form I( cbbL) and form II( cbbM)( that encodes Ribulose-1,5-biphosphate carboxylase/oxygenase( Rubis CO) were chosen as biomarkers in Calvin-Benson-Bassham cycle( a common carbon fixation pathway for chemolithoautotrophic microorganisms) to analyze the carbon sequestration gene diversity. The Illumina Miseq sequencing method and multivariate statistical analysis were employed. The cbbM gene was not detected in any of samples,while the cbbL gene was found in all samples. The cbbL-containing genetic communities were dominated by Proteobacteria,Cyanobacteria and Firmicutes,in which γ-Proteobacteria( 45. 3%),Cyanobacteria( 30. 9%),β-Proteobacteria( 23. 8%) were predominant subpopulation. There were differences between the nearshore station of A9 with other stations,in which Allochromatiu,Thiobacillus and Thiohalomonas were unique species in A9 station.Relativity and redundancy analysis suggest that water temperature, salinity, nitrate, nitrite, silicate were highly statistically significant factors.Temperature and salinity were positively( p < 0. 01),while the other three factors negatively influenced CO_2 fixation gene of cbbL gene abundance( p <0. 01).全球变化与海气交互作用专项(No.GASI-03-01-02-05);; 海洋公益性行业科研专项(No.201305030,201405007)~

21世纪中国金融学教学改革与发展战略

国家教育部新世纪教改工程项目《21 世纪中国金融学专业教育教学改革与发展战略研究》, 目前已取得阶段性成果。2001 年8 月16 日至21 日, 承担该项目的中央财经大学、中国人民大学、厦门大学和复旦大学在青海省西宁市举行了成果交流和研讨会。项目主持人、中央财经大学副校长、金融学博士生导师王广谦教授主持了这次会议, 全国所有有金融学博士学位授权点的高校和科研院所都有代表出席大会, 用主持人的话来说, “全国金融学界顶尖级人物基本上都到了”。出席会议的还有教育部高等教育司刘凤泰副司长和杨志坚处长。与会专家学者回顾了近20 年国内外金融业的迅猛发展, 分析了在21 世纪随着经济全球化、金融国际化对我国经济金融带来的机遇和挑战, 以及对我国金融研究、金融学科建设和人才培养带来的冲击,大家认识到, 经过20 年的改革开放和发展, 目前我国在这些领域虽有长足的进步, 但仍远远不能适应形势发展的要求。要想把我国金融学科建设成国际一流学科, 培养出一流的高素质金融专业人才, 还必须进行一系列改革与创新。与会专家教授根据各自的教学实践, 提出了许多具有建设性的意见。研讨会上发言踊跃、气氛热烈, 不时出现不同观念的碰撞和不同观点的交锋, 对推进金融学科建设十分有益。下面将著名专家教授颇具代表性的观点( 按发言顺序) 择要摘发, 以飨读者, 以推动中国金融学科的建设与繁荣