多波长萤光分光光度法(二)——萤光黄、罗丹明6G和罗丹明B三组分混合物的测定

本文叙述了应用多波长萤光分光光度法测定人工样品中的萤光黄、罗丹明6G和罗丹明B_。于波长502nm直接测量萤光黄的萤光强度,罗丹明6G和罗丹明B均不干扰;罗丹明6G的测定波长λ_1为555nm,参比波长λ_2和λ_3为502nm和660nm,罗丹明B的测定波长λ_1选用585nm,参比波长λ_2、λ_4和λ_3相应为538nm、508nm和494nm,方法的相对误差一般小于6%。作者于前文叙述了多波长萤光分光光度法的原理,为了验证该原理的可靠性,特对萤光黄、罗丹明6G和罗丹明B人工混合样品进行实测。结果表明,方法原理可靠,实验操作和数据处理简便快速,三次测定平均偏差<3%

抗H5亚型禽流感病毒单链抗体在毕赤酵母中的分泌型表达和生物活性分析

在本实验室研制出的多株针对H5N1亚型禽流感血凝素单抗中,13D4单抗对所有H5亚型病毒均有血凝抑制和中和活性,具有特异性高、反应性强和识别广的特点,且在小鼠实验中显示了对各种代表株禽流感的感染和发病均具有良好的治疗效果。在此研究基础上,本实验通过基因工程构建含有13D4单链抗体(scFv)基因的毕赤酵母表达载体,实现目的蛋白的分泌性表达和纯化。经过竞争法和血凝抑制检测其活性,表明获得的单链抗体具有与原始鼠源抗体相近的反应活性和相同的识别表位。H5N1广谱中和单抗13D4的单链抗体的成功构建,为进一步研制针对H5N1禽流感病毒的治疗性抗体奠定了基础

Development of aquantitative ELISA detection method for Varicella Zoster Virus(VZV) antigen

目的:建立水痘-带状疱疹病毒(VzV)抗原的双抗体夹心ElISA定量检测方法,用于质控VzV灭活疫苗研发和生产中抗原含量。方法:以VzV中和单抗5f6C8为包被抗体、8H5d1为酶标抗体,构建定量检测VzV抗原的双抗体夹心ElISA方法,并对本方法的特异性、灵敏度、准确性、线性和稳定性等性能进行分析。结果:建立的双抗体夹心定量检测VzV抗原的ElISA方法,线性范围为0.4μg~13μg/Ml,相关系数为r2=0.994,定量限度为0.4μg/Ml;变异系数CV80%。与VzV以外的相关病毒样本没有交叉反应。结论:构建的VzV抗原ElISA定量检测方法的各项性能符合定量检测需要,可用于VzV灭活疫苗的研发和生产过程的抗原含量检测。Objective:To develop a quantitative enzyme linked immunosorbent assay(Q-ELISA) to determine the concentration of Varicella Zoster Virus(VZV) antigen.This method was used to determine VZV antigen content at each stage of VZV inactived vaccine developing and manufacturing process.Methods: A double antibody sandwich Q-ELISA was developed to determine concentration of VZV antigen,which was based on the the high-affinity neutralizing monoclonal antibodies 5F6C8 as capture antibodies,and 8H5D1 as HRP-labeled antibody.The performance of reagent were evaluated.Results: The Q-ELISA for VZV antigen content was successfully developed.The reagent had good performance.The quantitation scope was 0.4 μg~13 μg/ml,The coefficient correlation was 0.994,the limit of detection was 0.4 μg /ml,the recovery was between 87.5% and 111.6%.The stability was up to 80% after reagent was heated for 6 days at 37℃.The variation coefficient was lower than 15%,and the reagent was no reaction with other sample except VZV antigen.Conclusion: The Q-ELISA for VZV antigen was developed with good specificity,accuracy and stability.The method can be used to determine VZV antigen content during development and production of VZV inactived vaccine

Thermogravimetric behaviors of biomass′ chemical components under air or syngas

在热重分析仪上,分别在空气和合成气气氛下,对生物质三种化学组分(纤维素、半纤维素和木质素)的热重行为进行了研究,考察了两种不同气氛下生物质及其三种组分的热化学转化温度区间分布;同时通过合成样品(以不同比例混合的三种组分的混合物)热重曲线的实验值和理论计算值的对比分析,考察了生物质化学组分在不同气氛下热重行为的相互作用.结果表明,在空气和合成气气氛下生物质各种化学组分发生热化学反应从难到易的顺序为木质素>纤维素>半纤维素,这是由其不同的化学结构决定的,但不同气氛下各组分的具体热重行为存在一定差别.在合成气气氛下木质素及半纤维素在与纤维素的共热解过程中存在明显的协同作用,而在空气气氛下半纤维素及纤维素在与木质素的共燃烧过程中存在一定的协同作用,这与各组分在不同气氛下所处的热化学反应温度区间具有一定的关系

氢气和合成气下生物质高压液化过程的实验研究

在小型高压反应釜中以四氢萘为溶剂，氢气和合成气为液化反应气，通过对不同液化条件下所得液化产物的收率及性质分析，考察了不同液化条件（反应温度、反应时间、反应气压力）对锯屑高压液化行为的影响；同时在相同液化条件下，通过液化产物收率和性质的分析，考察了气氛对锯屑高压液化行为的影响，探讨了用合成气代替氢气进行液化的可行性。结果表明，在氢气和合成气气氛下，随着反应温度的升高、反应时间的延长或反应压力的提高，液化油的收率都是增加的，但各种条件对液化油收率的影响程度不同。温度影响最大，时间影响次之，而液化气压力的影响最小。其他液化条件完全相同的情况下，氢气和合成气下所得产物的收率及性质相近，用合成气代替氢气液化具有可行性。在此条件下优化的液化反应条件为，以四氢萘为溶剂，反应温度为300℃，气体压力为2MPa，反应时间为30min，转化率为75．1％，液化油收率高达48．4％

STUDIES ON RATE of PHOSPHATE UPTAKE BY PHYTOPLANKTON IN XIAMEN HARBOUR

用无载体32P同位素示踪测定海水中浮游植物和细菌吸收磷酸盐速率的方法，对厦门港浮游植物和细菌进行了研究。结果表明，厦门港海水中吸收磷酸盐的主体是浮游植物（几乎占100%），平均吸收速率为4.28x10-5μMOl/（l·S），并且存在着晚秋＜冬＜初春的季节变化，培养实验的结果表明吸收速率的季节变化主要是由于种类更替和温度效应所致。A method For determining the rate of phosphate uptake by phytoplankton and bacteria in seawater using carrier-Free 32P radiotracer was established.The experiments were carried out From Nov.1988 to Mar.1989 in Xiamen Harbour, Fujian, China.The average rate of phosphate uptake by phytoplankton was 4.28 ×10-5μmol/(L· s);the turnover time was 0.51 day.In comparison to other regions, there were higher uptake rate and shorter turnover time of phosphate by phytoplankton in Xiamen Harbour.Results showed seasonal variations in the rate of phosphate uptake (late Fall<winter< early spring).Laboratory culture experiments indicated that the change was resulted From sucession of phytoplankton species and temperature.The uptake of inorganic phosphate was predominantly by phytoplankton, but not by bacteria in XiamenHarbour.高等学校博士学科点专项科学基金;福建省自然科学基

Study on Biochemical Effect of Cu~(2+) and Cd~(2+) on Porphyra haitanensis

研究了不同浓度(0.1、0.5、1.0、10、20 mg/L)和不同培养时间(12、24、48、72 h)下,重金属(Cd2+和Cu2+)对坛紫菜(Porphyra haitanensis)核酸(RNA和DNA)含量、过氧化物酶(POD)活性的影响.结果表明:1)10、20 mg/L Cd2+、Cu2+暴露,POD活性先上升(48 h前)后下降(48 h后),其它浓度组POD活性均随污染时间的延长而升高.24、48、72 h均出现比较良好的剂量 效应关系.2)Cd2+和Cu2+对RNA、DNA含量的影响基本上表现为先上升后下降的时间效应关系,同一污染物浓度越大越早出现下降.同时出现随暴露剂量的增加,RNA、DNA含量先上升后下降或全部下降的剂量 效应关系.Effects on nucleic acid (RNA and DNA) content and peroxidase (POD) activity of Porphyra haintanensis exposed to bivalent cadmium (Cd~(2+)) and bivalent copper (Cu~(2+) ) at concentrations of 0.1,0.5,1,10,20 mg/L were studied in experimental conditions.Samples were taken after exposure for 12 h,24 h,48 h and 72 h.Time-effect and dose-effect relationships of the nucleic acids and POD were investigated.The results showed:1)In groups exposed to 10 mg/L or 20 mg/L Cd~(2+) and Cu~(2+),the POD activity increased at first 48 hours,then decreased during the prolonging of the exposure time.In other dealing groups exposed to lower concentration of two heavy metals,POD activity increased throughout the exposing time.2)With the prolonging of exposure time, the effects of Cd~(2+) and Cu~(2+) on RNA and DNA contents in all group showed similar way:the content first increased and then decreased.And the higher the concentrations of the same pollutant,the earlier the decrease turned up.At the same time,there were significant dose-effect relationships between pollutants and RNA and DNA contents.The contents of nucleic acids increased first,then decreased or decreased completely with concentrations of two heavy metals increased.福建省重大科技项目(2001Z017

The Application of Sensory Stimulation in The Intervention of Alzheimer's Disease

阿尔茨海默病（Alzheimer&rsquo;s disease，AD）是一种神经退行性疾病，药物治疗是目前AD治疗的主要策略，但仅起到延缓或减轻作用。本文旨在综述各种感觉刺激和多感官刺激对AD的影响，并解释可能的机制，为进一步研究提供新思路。对光刺激疗法、气味刺激疗法、声音刺激疗法、节律性刺激、多感官刺激进行了系统分析，结果显示，这些感觉和多感官刺激可以有效改善AD的病理状态，唤起记忆，改善认知和行为。</p

濒危特有种掌叶木的微卫星遗传多样性研究

掌叶木居群具有较丰富的遗传多样性,该研究利用9对微卫星(SSR)分子标记揭示了掌叶木(Handeliodendron bodinieri)的遗传多样性。结果表明:观测等位基因数(Na)平均为3.903,有效等位基因数(Ne)平均为2.545,期望杂合度(He)平均为0.521,Shannon’s多态性信息指数(I)为0.962,PIC平均值为0.465。掌叶木的自然分布居群有相对较高的遗传多样性,但由于人为破坏等因素导致该群体濒危,而濒危并不是因为遗传多样性降低而造成的。居群间的遗传分化为掌叶木8个居群间的遗传一致度为(GI=0.849~0.970),遗传距离为(GD=0.032~0.164)。基于Nei’s遗传距离用UPGMA法对掌叶木居群进行聚类,Nei’s的基因分化系数为(G_(st))为0.027,平均Nei标准遗传分化系数(G'_(st)N)为0.031,平均Herick’s标准遗传分化系数(G'_(st)H)为0.064,基因流(N_m)为3.368。AMOVA分析结果表明:掌叶木居群间变异占3%,居群内变异占97%,居群内的遗传分化大于居群间的分化。利用Mantel检测发现,居群间的遗传距离与地理距离显著正相关(r=0.299,P<0.05)。该研究结果为掌叶木生物多样性和资源保护与利用提供了更充分的科学依据