To Compare Study on the the Cognition,Attitude and Behavior of Sexually Transmitted Diseases in Medical Students and Normal Students

目的比较医学生和师范生对性传播疾病STD防治知识的掌握情况以及对性行为态度及性行为的差异,为健康教育工作提供依据。方法自行设计性传播疾病KAP调查问卷,采用整群抽样的方法,对牡丹江师范学院和牡丹江医学院注册在读的学生进行问卷调查。结果医学生对STD相关知识的了解程度普遍高于师范生;医学生和师范生知晓性病的三大传播途径(性交、输血或血制品、孕妇传给婴儿)的比例差别无统计学意义(P>0.05),但在某些传播、非传播途径和预防性传播疾病知晓情况上,差异具有统计学意义(P0.05),but there were significant differences in the awareness of some transmission,non transmission routes andPrevention(P<0.05). CONCLUSION according to the needs of students in science students, the knowledge of sexual behavior andsexual behavior should be carried out to prevent sexually transmitted diseases

Expression and test of the neutralization Fab antibody against infectious bursal disease virus

为表达抗鸡传染性法氏囊病病毒(IbdV)抗体fAb并检测其中和活性,本研究将抗IbdV抗体的轻链(l)和重链片段(fd)基因分别克隆于P ET-27b(+)载体中,并转化于大肠杆菌rOSETTA(dE3)进行诱导表达。将l和fd片段包涵体蛋白变性后等量混合于复性液中,制备fAb并对其进行活性鉴定。结果显示l和fd蛋白相对分子质量大小分别为25 ku和28 ku。WESTErn blOT和ElISA检测结果表明,获得的抗体fAb大小约为50 ku,并且与VP2蛋白和不同病毒株均具有特异性结合能力。体外中和试验结果表明,获得的IbdV抗体fAb具有中和活性,可以有效阻断IbdV(b87株)对鸡胚成纤维细胞(df1)的感染。本实验获得的IbdV抗体fAb有望成为治疗Ibd的候选生物制剂,为研制治疗Ibd抗体制剂奠定了基础。To express the neutralizing Fab antibody against infectious bursal disease virus(IBDV),the gene of light chain(L)or heavy chain fragment(Fd) against IBDV was cloned into the prokaryotic expression plasmid,respectively,and then the recombinant L or Fd was expressed in E.coli Rosetta(DE3),respectively,and purified through sole denaturation and co-renaturation of inclusion body.Western blot results showed that the Fab was approximately 50 ku.ELISA results showed that the Fab exhibited binding ability and specify to VP2 for different IBDV strains.The results of neutralization test in vitro showed that the Fab exhibited neutralizing activity to IBDV-B87 strainin chicken embryo fibroblast(DF1) cells.The Fab antibody prepared in this study is expected to become a candidate drug for treatment of IBD,which laid the foundation for the treatment of IBD.黑龙江省应用技术研究与开发计划项目(GC13C104

新疆棉花可持续优质高产综合技术集成示范工程

该项目通过技术综合集成,分别形成早熟棉区、中低产棉区、风沙土棉区相应的100、120、150、200和250kg棉花优质高产栽培技术模式与配套技术体系;根据种植区光热、土壤条件,创立增株增铃为主的技术路线,将亩株数从认为已达极限的1.1万株增至1.8-2.2万株;利用农区多样性生物相生相克关系,首创种植苜蓿带防治棉蚜“生态棉田”新模式,实现无害化生产;根据新疆早熟棉区冬寒多雪,利用长效肥缓释特点,改基肥＋追肥的传统施肥为秋季一次性全耕层施肥技术;建立性诱剂、赤眼蜂、病毒杀虫剂、瓢虫扩繁等研制、开发及其综防体系,并加以应用;其脱落酸研究成果具有我国自主知识产权,在棉花种植中应用,可促进根系发育、..