Screen of O_2-tolerate Phenotype of Klebsiella oxytoca HP1 Mutants with High H_2-evolving Activity by Selection with MNZ Combination O_2

氢酶是生物制氢的关键酶,大多数氢酶因对氧极敏感而易失活,因此提高氢酶的氧耐受性对生物制氢有重要意义。本研究利用1%甲基磺酸乙酯对Klebsiella oxytoca HP1进行了两轮诱变,经40mmol/L甲硝唑和21%氧联合处理1h(第一轮诱变)或2h(第二轮诱变)进行筛选。所得突变菌株经产氢测试,结果在15%氧浓度条件下,第一代突变菌株HP1-A15产氢活性为出发菌株Klebsiella oxytoca HP1的3.70倍,在21%氧浓度条件下第二代突变菌株HPA15-37产氢活性为HP1-A15菌株的2.75倍,是出发菌株的11倍。突变菌株HP1-A15和HPA15-37具有较好的遗传稳定性。本试验结果说明利用MNZ和外加氧的方法适用于兼性厌氧菌耐氧产氢突变菌株的筛选。Hydrogenases are key enzyme for bio-hydrogen production, most of them were rapidly inacti-vated by oxygen. It is important to bio-hydrogen production and hydrogen application that improve the O2-tolerance of hydrogenase. In this experiment, the hydrogen producing strain Klebsiella oxytoca HP1 was treated with 1% ethyl methanesulfonate(EMS) , the mutants with high O2-toleration ability were screened with 40mmol/L metronidazole (MNZ) and 21% oxygen. The H2-evolving activity of the first generation mu-tant HP1-A15 was increased 3.70 times than that of the wild-type (WT) under 15% oxygen. The H2-evolving activity of the second generation mutant HPA15-37 was enhanced 11 times than that of WT under the condi-tion of 21% oxygen. The mutants HP1-A15 and HPA15-37 had steady heredity. These results suggest that MNZ and in addition oxygen is a good way to screen of O2-tolerate phenotype of facultativeanaerobe withhigh H2-evolving activity.福建省青年科技人才创新项目(No.12005J003);; 厦门市科技项目(No.13502Z20041070

Klebsiella oxytoca HP1 adhE基因插入失活法构建产氢重组菌

乙醇是产酸克雷伯氏菌(Klebsiella oxytoca)HP1厌氧发酵产H2的主要副产物,每生成1.0 mol的乙醇需要消耗2.0 mol NAD(P)H,从而降低了H2的产量.本研究以编码乙醇脱氢酶系(含乙醛脱氢酶和乙醇脱氢酶活性)的adhE基因为改造目标,利用同源重组技术获得了以提高产氢为目标的K.oxytoca重组菌.构建工作包括:根据adhE基因保守序列框克隆K.oxytoca HP1 adhE基因片段,以质粒pMHE6为模板进行链霉素抗性基因表达盒的扩增,表达链霉素抗性的aadA基因片段和adhE基因片段分别与载体pMD18-T相连构建重组质粒,同源整合质粒pTA-Str的构建,以链霉素作为筛选标记筛选重组菌.菌落PCR鉴定结果表明,aadA基因表达盒通过质粒pTA-Str的介导已定点插入K.oxytoca HP1基因组中,成功地构建了adhE基因部分片段缺失的重组菌.葡萄糖发酵实验结果表明,相同发酵条件下,重组菌比野生菌的产氢量提高了16.07%,乙醇产量下降了70.47%.利用基因工程技术提高产氢初步获得成功.国家自然科学基金(批准号:30470395);; 福建省重点科技项目(批准号:2005I106

The Current Situation and Protection Measures of Amphibians and Reptiles Diversity of Huaihe River basin in Anhui

经过调查统计,发现淮河流域(安徽段)现有两栖动物11种,其中古北界2种,东洋界5种,广布种4种;爬行动物23种,属于古北界4种,东洋界13种,广布种6种。区系特点明显,属于古北界向东洋界的过渡区,东洋界和广布种种类分布相对丰富。相似系数分析表明沱湖和女山湖相似系数最高,说明其生境和其他两个保护区相比更为优越。此研究有助于了解该地区两栖爬行动物的多样性现状,为进一步加强资源的保护和可持续利用提供参考。The amphibians and reptiles diversity of Huaihe River basin in Anhui has been investigated and studied.The result indicates that 11 amphibian species have been found,2 of them are pale arctic realm,5 oriental species and 4 widespread species.23 reptile species have been found,4 of them are pale arctic realm,13 oriental species and 6 widespread species.The fauna feature was obvious.The pale arctic species are seldom,the oriental species and the widespread species are rich.Analysis indicates that the similar coefficient of Tuohu and Nvsanhu are the highest.The conclusion is that the diversity of amphibians and reptiles in this area can be illustrated;the environment and wildlife resources can be utilized reasonably after more protection.安徽科技学院重点学科建设基金项目(YZD2004-19)资

塞克硝唑片治疗滴虫阴道炎的随机对照多中心临床研究

【目的】评价口服塞克硝唑片治疗滴虫阴道炎的有效性和安全性。【方法】采用多中心、随机双盲、平行对照设计，入选滴虫阴道炎患者144例，塞克硝唑片组与替硝唑片组各72例，对其临床疗效和滴虫清除率进行比较，并观察用药后不良事件和实验室检查异常的发生情况。【结果】用药后塞克硝唑片组痊愈率和有效率分别为61％和94％；替硝唑片组痊愈率和有效率分别为51％和94％，两组之间比较差异无统计学意义（P〉0．05）。塞克硝唑片组滴虫清除率为96％；替硝唑片组滴虫清除率为97％，两组比较差异无统计学意义（P〉0．05）。144例病例中无不良事件与严重不良事件发生；塞克硝唑片组实验室检查异常发生率为0，替硝唑片组实验室检查异常发生率为4％，两组之间比较差异无统计学意义（P〉0．05）。【结论】口服塞克硝唑片治疗滴虫阴道炎安全、有效

Study on Hydrogen Production Activity of Klebsielal oxytoca HP1 and Stability of Its Soluble Hydrogenase under Atmosphere with O_2

目的:考察产酸克雷伯氏菌(Klebsielal oxytoca HP1)耐氧产氢特性及其可溶性氢酶的氧耐受特性。方法:研究K.oxytoca HP1在不同气相氧浓度条件下利用葡萄糖(1%,m/v)、丙酮酸钠(0.5%,m/v)及甲酸(0.1%,v/v)等底物产氢活性的以及K.oxyto-ca HP1可溶性氢酶在空气及氧饱和溶液中催化产氢活性。结果:K.oxytoca HP1在葡萄糖(1%,m/v)底物中具有较高耐氧产氢活性,6h内在气相氧浓度为5%、10%和21%条件下的氢产量分别为厌氧条件下的20.9%、13.7%、8.3%;K.oxytoca HP1可溶性氢酶在空气中孵育12h后,其活性残余85.4%,在氧饱和溶液中活性损失一半约3h。结论:试验结果提示K.oxytoca HP1具有耐氧产氢特性,其可溶性氢酶具有较高氧耐受性,在氢能源的开发中具有潜在的应用前景。Objective: To study the hydrogen production activity of K. oxytoca HP1 under condition with oxygen stress and the oxygen tolerance ability of its soluble hydrogenase. Method: The hydrogen production activity of K. oxytoca HP1 incubated in glucose (1%,m/v), sodium pyruvate (0.5%,m/v)or formate (0.1%,v/v) under atmosphere with 0%,5%,10% and 21% O2 were studied on, and the remain activity of its soluble hydrogenase incubated in air or pure O2 during 0-12h were studied on. Result: The results show that K. oxytoca HP1 has high hydrogen production activity in glucose (1%,m/v) substrate under atmosphere with oxygen. Compared to in anaerobic condition, the hydrogen production of K. oxytoca HP1 under atmosphere with 5%, 10% and 21% oxygen was 20.9%,13.7% and 8.3% respectively in 6h. The soluble hydrogenase from K. oxytoca HP1 exhibits considerable oxygen toleration ability, the remain activity was 85.4% after the hydrogenase exposure to air 12h and its activity lost half after the hydrogenase exposed to oxygen 3h. Conclusion:These results suggest that K. oxytoca HP1 and its soluble hydrogenase have potential to be applied in hydrogen production and application.福建省青年科技人才创新项目资助(No.12005J003

Comparative double-blind trial of KN-7 tablet and Robaveron injection in the treatment of neurogenic bladder

37施設で, 神経因性膀胱233例を対象に, ロバベロン注射剤を標準薬とし, KN-7錠剤の経口投与による排尿障害に対する有用性を, 二重盲検群間比較試験で比較検討した.解析対象例はKN-7群108例, ロバベロン群107例である.KN-7群は1日6錠投与した.改善度の効果判定では改善以上K群40.7%, R群38.7%, やゝ改善以上それぞれ76.9%, 72.6%と有意差はなかった.臨床所見中改善の良かった項目は, 尿意, 残尿感, 尿失禁, 排尿までの時間, 排尿時間, 尿勢-排尿力と尿線中断の改善などで, これらの改善以上はK群35.8%, R群30.8%で, 有意差はなかった.副作用はK群0.9%, R群6.8%, K群が有意に少なかった.両剤とも主として下痢で, R群ではその他頭痛, 頻脈, 肝機能障害, 発熱感などを少数例認めた.臨床検査値ではGOT, GPTの上昇が2例あった.有用度有用以上はK群46.3%, R群45.8%で有意差はなかった.疾患別では, 末梢神経疾患, 膀胱の性状では弛緩性, 時期では12ヵ月以内の例で特に改善率が高かった.以上から, KN-7錠剤は, ロバベロン注射剤に比べ, 同等の効果が期待でき, 安全性も高く, 長期投与あるいは外来患者の治療にも適するThe clinical effectiveness, safety and usefulness of KN-7 tablet as a new oral application of the prostatic extract, on urinary dysfunction of neurogenic bladder were compared with those of Robaveron injection by the double-blind test method. In the study, 2 tablets t.i.d. and a shot of intramuscular injection 1 ml a day were given successively for 3 weeks. A total of 233 cases were reported from 37 facilities belonging to the KN-7 Clinical Research Group. Some of them were excluded or dropped out. The number of cases used for analysing the effectiveness, safety and usefulness were 214, 232 and 215, respectively. There was no bias between the two groups with a significant homogeneity in the background. In the overall clinical effectiveness, the effective rate including excellent, moderate and slightly effective was 76.9% with KN-7 and 77.4% with Robaveron. In the clinical usefulness, the rate of usefulness of slightly useful or above was 75.0% with KN-7 and 75.7% with Robaveron. There was no significant difference between the two groups in the clinical effective and useful rates at a significant level of 5%. Side effects were observed in 1 of the 114 (0.9%) patients given KN-7 and 8 of the 118 (6.8%) patients given Robaveron. The incidence of adverse reactions with KN-7 was significantly lower than that with Robaveron. Based on the results, it was concluded that KN-7 tablets, 2 tablets t.i.d., would be as effective and useful as a Robaveron injection 1 ml daily and safer than the latter in the treatment of neurogenic bladder

定額寺制の成立について / 東北地方における近世大名の成立 : 伊達氏の場合 / 藤田幽谷の経済思想 : 特に勧農或問を中心に / 水戸藩に於ける郷校の一研究 / 笠間班における藩政改革 / プセウドクセノフォン「アテナイ人の国制」をめぐる諸問題 / 農民層分解を通してみた第一次綜劃運動について / レッシングの啓蒙思想 / 一八三〇年代初期におけるイギリス労働者の動き : 建築工組合を中心に

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能登半島地震前後の環境放射能の変動 (<特集>2007年能登半島地震 その2)

金沢大学環日本海域環境研究センターVariations of radiation levels before and after the Noto Hanto Earthquake in 2007 have been analyzed from the point of view of environmental radioactivity. The 210Pb activities in the airborne particles are collected from Nishi-Futamata in Wajima, are analyzed. The Radon activity at Hegura Island located 50km North of Wajima, and the spatial gamma rays at Nishi-Futamata are measured since April 21, 2007. Abnormal increase of 210Pb activity started 3 weeks before the earthquake are found, suggesting the maximum value just before the earthquake, because it decrease to normal level after 2 weeks. Increase of radon level was not observed at Hegura Island, however, increase of radiation level at Nishi-Futamata area continued for 6 weeks after the earthquake, and settled to normal level in middle of May 2007.環境放射能の観点から能登半島地震発生前後の放射能関連のデータの解析を試みた.解析したのは,輪島市西二又地区で採取した大気浮遊塵中の210Pb,輪島沖50kmに位置する舳倉島のラドン濃度,地震発生後の4月21日から西二又地区で連続測定を実施した空間γ線レベルの3項目である.その結果,地震発生約3週間前から大気浮遊塵試料のラドンの娘核種210Pbの濃度が増加し,地震直前にピークに達した後に低下に転じ,約2週間後にほぼ平常値に回復していたことが分かった.舳倉島のラドンには地震の影響は見られなかったが,西二又における空間γ線レベルはラドンに由来すると考えれる高い値が約6週間後も続き5月中頃に平常値に戻ったことが明らかになった

A Study of Settlement Remains near the Qiemo Oasis in Northern China using Satellite Imagery and DEM

In this paper, we offer our study results about the location and structure of remains of ancient settlements in an arid region using satellite imagery and DEM. After our overview of the study area, namely the Qiemo (Cherchen) in the Tarim Basin, northwestern China, we examined the settlement remains over a desert area extending southwestward from the present town of Qiemo. A network of remains of irrigation canals, which take the shape of ridges, was observed both on the ground and on the satellite imagery. We made a reconstruction of the ancient irrigation network, and examined its geomorphological context. We also discovered an interesting coincidence of the canal remains and the subtle elevations observable in the SRTM DEM. In conclusion, we offer a hypothesis that a powerful flood eroded the settlement of the settlements.departmental bulletin pape