Matrine Induces Apoptosis of Hep G2 Cells via Mitochondrial Apoptotic Pathway

目的探讨苦参碱通过线粒体通路诱导HEP g2发生凋亡的机制。方法分别采用MTT法、PI染色法、流式细胞术检测苦参碱对肝癌HEP g2细胞的增殖抑制、周期调控、诱导凋亡作用;采用WESTErn blOT检测苦参碱诱导肝癌HEP g2细胞抑凋亡蛋白bId、bCl-2的表达量。结果苦参碱可抑制HEP g2细胞的增殖作用和诱导凋亡,呈时间和剂量依赖性,并可将HEP g2细胞周期阻滞在g0/g1期。苦参碱可引起线粒体膜电位崩溃,同时诱导bId、bCl-2表达下调,bAX表达上调。结论苦参碱通过调节细胞内bId、bCl-2和bAX蛋白的表达,经线粒体信号转导途径诱导人肝癌细胞系HEP g2发生凋亡。Objective To explore the mechanism of matrine inducing apoptosis of Hep G2 cells via mitochondrial apoptotic pathway.Methods MTT assay,PI staining,flow cytometry were used to evaluate the anti-proliferation,cell cycle regulation,and inducing apoptosis effects of matrine on Hep G2 cells.Western Blot assay was used to detect the expression levels of anti-apoptotic protein Bid and Bcl-2 in Hep G2 cells.Results Matrine could inhibit the proliferation and induce the apoptosis of Hep G2 cells in a time- and dose-dependent manner,and the cell cycle of Hep G2 was arrested in G0 / G1 phase.Apoptosis of Hep G2 cells induced by matrine was associated with collapse of mitochondrial membrane potential,which made the Bid and Bcl-2 expression levels down-regulated but the Bax expression level up-regulated.Conclusion Matrine induces the apoptosis of human hepatoma cell line Hep G2 via the mitochondrial apoptotic pathway by regulating the expression levels of Bid,Bcl-2 and Bax proteins.国家自然科学基金资助项目(81172284); 福建省自然科学基金资助项目(2010J0615

The Morphology of the Primary Dental Arch of Chinese Children in Shijiazhuang-City Part-I: Research concerning the size of the primary tooth crown, primary dental arch and the condition of primary occlusion

The purpose of this study was to investigate the size of the primary tooth crowns, primary dental arches, standard values and frequency distribution of primary occlusion in Chinese children. With the cooperation of a kindergarten in Shijiazhuang-city, China, the dental plasters from 55 children (36 boys and 19 girls, age range from 3-6 years old) with normal primary occlusion were collected. Because of different growth rates of the children, these children were divided into two groups, one comprising children less than 5 years old and the other of 5 years old or older. According to the method that the Japanese Society of Pediatric Dentistry reported, the data were statistically analyzed and compared to Japanese children. The results were summarized as follows: 1. Except lower primary lateral incisors, the values of the mesio-distal width of primary crowns of each tooth were significantly larger for boys than for girls in all tooth types. Also the Chinese children were inclined to show smaller mesio-distal tooth crown width than those of the Japanese children in all types of teeth. 2. Compared with the Japanese children, the Chinese children were inclined to show smaller primary dental arch length both in the maxilla and the mandible. But in the senior group, the Chinese girls were inclined to show smaller primary dental arch width than their Japanese female counterparts. On the contrary, the Chinese children tended to show larger dimensioned primary dental arch height than those of the Japanese children. 3. Though spaces between teeth (primate and growth spaces) were found in the Chinese children, the frequency was lower than that of the Japanese children. 4. The frequency of terminal plans in the Chinese children was 41.8% in the vertical type, 6.4% in the distal-step type and 51.8% in the mesial step type. 5. The primary canines occlusal relationship in the Chinese children was 63.1% in type I, 13.6% in type II and 23.7 in type III

Screening of Strains Fermenting Ethanol with Bagasse Saccharification Mixture

利用纤维生物质生产生物燃料是解决当今世界能源和环境问题的重要途径.本研究从腐烂蔗渣、酒曲、酒糟等样品中分离到一批产乙醇菌株,通过高浓度乙醇选择性培养基筛选、TTC法复筛以及发酵乙醇能力分析,筛选得到一株发酵蔗渣糖化液能力较强的菌株.通过对菌落形态观察、电镜图片分析、18S rDNA基因序列分析,确定该菌株属于酿酒酵母属,命名为Saccharomyces cerevisiaeQ2-1.优化了S.cerevisiaeQ2-1发酵蔗渣糖化液产乙醇条件,当起始pH为5.5、发酵温度为30℃、糖化液中硫酸铵浓度为0.4%时,静置发酵60 h后,发酵率可达89.2%,蔗渣产乙醇率ω=13.6%.Several strains ferment ethanol using bagasse saccharification mixture were isolated from rotted bagasse and lees.The strain with highest ethanol yield was screened and identified as Saccharomyces cerevisiae Q2-1 by analysis of morphology and 18S rDNA sequences.The optimum conditions for ethanol production using bagasse saccharification mixture were obtained as: using 0.4 %(NH4)2SO4 as nitrogen source,fermentation temperature 30℃,pH 5.5,fermentation time 60 h.The maximum zymolysis ratio was achieved as 89.2% with an ethanol yield of 13.6%(by mass).福建省重点科技平台项目(2006H0091);; 国家基础科学人才培养基金(J0630649)资

Species composition and age structure of fish in the Daya Bay in spring and autumn

2006年4月和9月对大亚湾海域通过拖网和流刺网捕获的鱼类进行初步调查.结果表明,渔获物中出现18目76科135属200种鱼类,其中软骨鱼纲4目5科5属6种,占调查鱼类总种数的3.0%;硬骨鱼纲14目71科130属194种,占调查鱼类总种数的97.0%.根据其适温类型可分为暖水性种、暖温性种和冷温性种,其中暖水性鱼类有183种,占总种数的91.5%;暖温性鱼类有16种,占总种数的8.0%;冷温性鱼类仅有1种,占总种数的0.5%.渔获物中底层和近底层鱼类占多数,其中,底层鱼类共有74种,占总种数的37.0%;近底层鱼类共有68种,占总种数的34.0%;中上层鱼类共有45种,占总种数的22.5%;岩礁鱼类有13种,占总种数的6.5%.大亚湾鱼类的年龄普遍偏低,以1 A龄鱼为主.与相邻海区鱼类区系特征比较,大亚湾海域具有我国热带和亚热带性海湾的鱼类区系特征.An investigation of fish in Daya Bay was conducted in April and September 2006 using a trawl and drift net.The results showed that there were 200 species of fish belonging to 135 genera in 76 families of 18 orders.of these,6 species in 5 genera,5 families,and 4 orders belonged to Chondrichthyes,and 194 species in 130 genera,71 families,and 14 orders belonged to Osteichthyes,accounting for 3.0% and 97.0%,respectively.Based on the temperature nature of the fauna,the fish in Daya Bay may be grouped into three types,with warm-water species,warm-temperature species and cold-temperature species accounting for 91.5%(183 species),8.0%(16 species) and 0.5%(one species),respectively.According to the diversity of the habitable environment,demersal fishes were the most abundant,accounting for 37.0%(74 species),followed by near demersal and pelagic fish,accounting for 34.0%(68 species) and 22.5%(45 species),respectively,and reef fish only accounted for 6.5%(13 species).The age structure of the fish primarily consisted of one age class in Daya Bay.When compared with other sea areas around Daya Bay,most species of ichthyofauna in the South China Sea bear tropical and subtropical characteristics.广东省科技计划资助项目(2004A30501002

Realization of Combined Machining System for Coated Grain and Research on Drilling Technology

本文对系统组成、关键技术实现方法等进行阐述,并对钻孔工艺进行研究且对钻孔温度以及药柱无损夹持进行仿真。自由填装式包覆药柱整形操作主要靠手工整形,其操作方法落后,存在较大的安全隐患,且质量一致性较差,生产效率低,已成为制约发展的瓶颈问题。针对上述问题,研制了一种能够实现包覆药柱端面切割与打孔的自动化整形智能成套系统,确保包覆药柱整形的操作安全性,保证产品质量,提高整形作业效率,减轻工人的劳动强度,以满足大批量生产和未来发展的需求

Research on the Recognition of the Position of the Silver Wire of Grain Based on Multiple Algorithms

提出了一种图像特征点筛选提取的方法对药柱中银丝位置进行快速有效的精确识别定位,该方法在对CCD相机获取的药柱端面图像进行二值化预处理的基础上,通过边缘检测与椭圆拟合结合的方式,提取环形ROI区域,并利用标记法比对ROI区域内各特征区域像素个数,排除干扰区域,最后采用贪心算法,比对剩余特征区域的中心坐标之间距离,最终得到银丝位置坐标;该方法有效的排除了药柱端面的干扰特征,实现快速准确的提取符合银丝位置的特征区域,满足批量生产的要求