脲型手性固定相高效液相色谱拆分苯基琥珀酸对映体

国家自然科学基金资助项目(No.20072031)

High Performance Liquid Chromatographic Separation of Racemic Phenylsuccinic Acid Using Chiral Mobile Phase Additives

以七 (2,3,6_三_O_甲基 )_β_环糊精 (TM_β_CD)作为手性流动相添加剂 ,反相高效液相色谱研究苯基琥珀酸 (PSA)对映体拆分 ;在Nova-pakC18 色谱柱上 ,采用0.30mmol/LTM_β_CD、含0.05 %三氟乙酸的乙腈 -水(体积比16∶84)为流动相 ,(R)_( -)_PSA和(S)_( +)_PSA的容量因子分别为5.43和6.42 ,对映体分离因子为1.18 ,分离度为2.50 ;对比PSA在 β_CD手性流动相法和2,6_丁基化_β_CD涂渍C18柱的色谱行为 ,探讨环糊精分子对PSA的手性拆分机理 ;本法已用于测定L_脯氨酸化学拆分苯基琥珀酸对映体产品的光学纯度。国家自然科学基金资助项目(２００７２０３１;２０２７２０４８;２０３９００５０５

Domestication of the high-sugar-tolerant Mortierella alpina on arachidonic acid(ARA) production

通讯作者：Tel: 86-592-2186038; : [email protected][中文摘要]【目的】提高花生四烯酸(Arachidonic acid,ARA)产量,克服ARA产生菌高山被孢霉(Mortierella alpina)在长期的保存及使用过程中易受到外界条件影响发生退化,从而导致菌种耗糖量降低、影响菌种摄入营养的能力和不利于工业化生产的缺点。【方法】首先采用固体培养基驯化,将菌种逐级涂布于梯度高糖PDA平板(含糖量分别为2%、5%、7%、10%和15%)培养,挑选经固体驯化后能耐受10%高糖浓度平板的菌种,转接到两种含不同氮源的梯度高糖(含糖量分别为3%、4%、5%和6%)液体培养基中进行驯化,最后对驯化后的菌种进行2 L发酵罐放大实验。【结果】当培养基中以酵母粉为氮源时,驯化后菌体的最高耗糖量由3 g/(L.d)提高到12 g/(L.d);当培养基中以玉米浆为氮源时,驯化后菌体的最高耗糖量由7 g/(L.d)提高到12 g/(L.d)。摇瓶驯化实验结果表明以玉米浆为氮源驯化的菌种发酵效果较好,发酵罐实验结果显示菌体生物量为50 g/L,总油脂为18 g/L,目的产物ARA产量为8 g/L。相比未驯化之前的发酵结果,生物量和总油脂含量提高了近3倍,ARA产量提高了近4倍。【结论】经过高糖驯化, 菌种的耗糖能力得到提高, 生物量、总油脂及ARA 的产量也都有所增加, 从而可以使菌种在保存和使用过程中不易退化,保持稳定。[英文摘要][Objective] In order to improve arachidonia acid(ARA) production,and prevent the degeneration of ARA-producing strain Mortierella alpina in long-term culture preservation and cultivation,which could lead to low consumption rate of substrates like carbon source.[Methods] Mortierella alpina strain was first domesticated in high-sugar PDA plate with gra-dient sugar content(2%、5%、7%、10% and 15%).The strain which grew better in the solid medium containing 10% sugar was then selected and transferred to two liquid high-sugar media with different nitrogen sources to domesticate. The gradient sugar contents were 3%、4%、5% and 6%, respectivlely. Finally, the domesticated strain was used to produce ARA in a 2 L bioreactor. [Results] The experimental results show that the consumption of sugar increased from 3 g/(L·d) up to 12 g/(L·d) in the medium with yeast extract, and from 7 g/(L·d) up to 12 g/(L·d) in the medium with corn steep liquor. After cultivation for 144 h in a 2 L bioreactor,the yield of ARA reached 8 g/L, which was 4 times higher than that obtained with the original strain. [Conclusion] The domestication of the high-sugar-tolerant Mortierella alpina increased the consumption of sugar, biomass, total fatty acid and ARA production. The domesticated strain could maintain the high sugar resistance in the preserving process and turn to be more stable.福建省科技厅重大专项项目(No.2010NZ0001-4);; 国家自然科学基金项目(No.31071488

A Silver Nanoparticle-Enhanced Fluorimetry for Determination of Trace Enrofloxacin in Water

在实验条件下建立了水溶液中痕量恩诺沙星的荧光分析方法;在课题组已有工作的基础上,采用“绿色“化学方法合成了银纳米粒子;研究了所制得的银纳米粒子对水溶液中恩诺沙星荧光行为的影响,并最终建立了水溶液中痕量恩诺沙星的银纳米粒子增强荧光分析方法。Firstly, a fluorimetry for determination of trace enrofloxacin in water was established in laboratory conditions.Secondly, the silver nanoparticle was synthesized based on our previous studies.In addition, effect of silver nanoparticle on the fluorescence behavior of enrofloxacin in water was investigated.Finally, a silver nanoparticle-enhanced fluorimetry for determination of trace enrofloxacin in water was also established.国家自然科学基金项目;项目号:21207103; 浙江省大学生科技创新活动计划暨新苗人才计划;项目号:2013R413010; 浙江省公益项目;项目号:2012C31025

Enantiomeric Separation of Mandelic Acid and Its Analogues by High Performance Liquid Chromatography with 2,6-Di-O-Pentyl-β-Cyclodextrin Coated Symmetry C_8 Column

利用合成的 2 ,6 二 O 戊基 β 环糊精涂渍SymmetryC8色谱柱 ,研究了扁桃酸及其类似物等 6种外消旋对映体的反相高效液相色谱拆分。优化了色谱分离条件 ,探讨了扁桃酸的手性拆分机理。结果表明 ,采用优化后的甲醇 水或甲醇 0 5 %三乙胺 乙酸缓冲液流动相等色谱条件 ,扁桃酸、扁桃酸甲酯、苯基甘氨酸、苯基琥珀酸和安息香等 5种外消旋对映体达到或接近基线分离 ,其中前 4种对映体均为 (S) 构型先出峰。该法可用于实际样品的对映体纯度测定。国家自然科学基金资助项目(Nｏ.20272048,203900505

Preparation of mesoporous silica nanoparticles in different pore size and its use in the solidification of sirolimus loaded self-microemulsifying drug delivery system

研究制备了不同孔径的介孔二氧化硅纳米粒及西罗莫司自微乳。使用扫描电镜、透射电镜、小角度X-射线衍射、氮气吸附-脱附表征制得的介孔二氧化硅纳米粒,发现其为有序的纳米孔道结构,孔径分别为6.3、8.1和10.8 nm,通过粒径测定仪测得西罗莫司自微乳粒径为20.6±1.3 nm。通过搅拌法制备载西罗莫司自微乳介孔二氧化硅纳米粒。研究发现,当西罗莫司自微乳与介孔二氧化硅纳米粒质量比2∶1时为最佳比例,载药量约为0.83%,且固体粉末流动性良好。采用差示扫描量热法表明,西罗莫司以非晶体或无定形存在于载体材料中,重分散性良好。孔径大小对于载西罗莫司自微乳介孔二氧化硅纳米粒在250 m L水中2 h的释放终点并没有显著影响,但对于最初40 min释放结果影响较大。综上,介孔二氧化硅纳米粒有望成为一种新型的自微乳吸附剂。The mesoporous silica nanoparticles (MSN) in different pore size and sirolimus (SRL) loaded self-microemulsifying drug delivery system (SMEDDS) were prepared. The results in morphology were collected by scanning electron microscope, transmission electron microscope, small-angle X-ray diffraction, and N2 adsorption-desorption. The results showed that the prepared MSN has ordered nanochannels with a pore size of 6.3, 8.1, 10.8 nm, respectively. The particle size of SRL-SMEDDS were measured by particle sizing system, which was 20.6 ± 1.3 nm. The stirring method was developed to prepare SRL-SMEDDS-MSN. It was found that the optimal ratio of SRL-SMEDDS to MSN was 2: 1, while the drug loading rate was near 0.83%, and the flow properties of SRL-SMEDDS-MSN were of good condition. The differential scanning calorimetry results proving a molecular or amorphous dispersed state of SRL in MSN while the suspension experiment has shown great reconstitution properties of SRL-SMEDDS-MSN. There is no significant influence on maximum drug release rate of different pore size of SRL-SMEDDS-MSN in 250 mL water within 2 h, while the results of the first 40 min have an obvious difference. Above all, MSN might provide a new strategy for the solidification of SMEDDS.福建省科技计划重点资助项目（2013Y0074

The Preparation of Silver Nanoparticles

［中文文摘］在简要介绍纳米银在各个工业领域应用的基础上,总结比较了各种制备纳米银颗粒的方法。物理和化学方法的工艺技术都比较成熟,但也存在着一定的不足。新兴的生物还原法因其具有微生物原料来源广,生物还原反应条件温和,产物纳米颗粒不易团聚,以及过程加入的化学试剂和产生的有毒副产物少等特点而开始受到关注。微生物还原金属离子有2种不同的机理:微生物的酶催化机理和非酶还原机理。对生物还原法原理的充分认识是将该方法发展成为可实际应用的纳米银制备工艺的重要基础。［英文文摘］The main applications of silver nanoparticles in industry were briefly reviewed in the present paper. The methods used for preparation of silver nanoparticles were summarized and compared. The physical and chemical methods are relatively mature but they have some shortcomings. The biological method is recently developed as a promising method because of its special advantages such as sufficient material sources, mild reaction conditions, good dispersion of nanoparticles as well as few chemical addictives and poisonous byproducts. The biological method for preparation of silver nanoparticles included two mechanisms, namely enzymatic catalysis mechanism and non-enzymatic reduction mechanism. The full understanding of two mechanisms would be necessary for developing it into a practical process to prepare silver nanoparticles.国家自然科学基金(20376076); 中国石油化工股份有限公司项目(0041-K81042)

一种聚变反应堆装置的新概念

本文提出了一个聚变反应堆装置的新概念,它由两个轴对称的类似串联磁镜的部分和两个螺旋仿星器U形弯曲段组成,并综合了直管和环形、快过程和慢过程的优点。文中研究了起动、过渡和运行阶段,讨论了D-D反应堆参数和稳定性。射频和高能分量只在起动阶段才需要,在整个过程中只有瞬时的能量消耗。端塞也只须在起动阶段存在,这大大降低了工程技术要求