荷塘诗集

《荷塘诗集》十六卷,清张五典著。清乾隆刻本。八册。半叶九行十九字,左右双边,黑口,单鱼尾。框高17.1厘米,宽12.2厘米。题＂泾阳张五典叙百＂。前有乾隆五十二年（1786）金汝圭序、乾隆四十二年（1777）李汪度序、乾隆五十年（1785）朱珪序、乾隆五十年徐立纲序

The Analysis of Regional Effects about Industrial Clusters and Theirs' Design of Indicator System

文章从集群效应的涵义出发,分析集群效应的种类及其相互关系,并指出影响集群效应的四大因素,最后构建了集群效应的指标体系。In this paper is mainly proceeding from the meaning of regional effects about industrial clusters, made analysis with the types of effects and their mutual relations, and then pointed out the four factors which impact the effects, finally taken a Construction with the indicator system of effects.广西教育厅项目“广西新兴城市产业集群发展研究”(200702MS121)研究成果;广西师范大学广西人文社会科学发展研究中心“边疆问题研究”专项课题资助项目“桂西南民族边境地区糖业集群发展建设对策研究”阶段性成

1,2-二苯基乙二胺及与脱氢乙酸缩合生成的席夫碱化合物对映体的高效液相色谱法拆分

福建省自然科学基金资助项目(C0510001

Study on Corrosion Resistance of Zr-0.8Sn-1Nb-0.3Fe Alloy after Kr~+ Ion Irradiation

采用高压釜腐蚀实验研究了2种不同制备工艺下的Zr-0.8Sn-1Nb-0.3Fe合金(1#,2#)经360℃、5~25; dpa的Kr~+辐照后、在400℃/10.3 MPa过热蒸汽中的耐腐蚀性能,用透射电子显微镜(TEM)、扫描电镜(SEM)、; X射线衍射仪(XRD)分析合金腐蚀后氧化膜显微组织结构。结果表明,100; d腐蚀后,合金的腐蚀增重随着辐照剂量的增加而增加,由于1#合金中的第二相比2#合金更为细小、弥散,相同辐照剂量下,前者的腐蚀增重较低。腐蚀转折前; ,从蒸汽腐蚀侧到锆合金基体,氧化膜中的氧含量逐渐降低,靠近蒸汽侧的氧化膜主要由等轴晶形态的单斜ZrO_2组成,而基体界面处的氧化膜主要为柱状晶形; 态的四方ZrO_2和六方Zr_3O;腐蚀转折后,基体界面处的氧化膜呈"花菜"状生长,"花菜"尺寸大小与氧化膜生长速率的高低及不均匀生长趋势的大小; 呈对应关系。The corrosion resistance of Zr-0.8Sn-1Nb-0.3Fe alloys prepared by two; different processes was investigated in 400℃/18.6MPa superheated steam; by static autoclave after irradiated by 360℃ with Kr~+-irradiation of; 5~25 dpa. The microstructures of oxidation film after corrosion were; analyzed by TEM, SEM, and XRD. The results showed that the corrosion; weight-gain increased with the irradiation dose, while the weight-gain; of 1# alloy with smaller and more dispersive SPPs than 2# alloy was; lower under the same irradiation dose. Before corrosion turning, the; oxygen content in the oxidation film decreased from the steam-side to; the zirconium matrix. The oxidation film beside the steam-side was; mainly composed by equiaxied monoclinic ZrO_2 crystal, while near the; film/matrix interface by columnar quartet ZrO_2 crystal and hexagonal; Zr_3O crystal. After transition of corrosion weight, the film near the; interface grew like cauliflowers, and the size of cauliflowers were; corresponded to the growth rate and uneven growth trend of oxidation; film

人乳头瘤病毒假病毒制备方法的优化

人乳头瘤病毒假病毒可广泛应用于中和抗体鉴定和疫苗免疫性评估等研究.本研究对多质粒共转染293FT细胞生产HPV假病毒的方法进行了深入优化,结果显示不同的HPV结构蛋白表达质粒转染比例对于假病毒生产效率有显著的影响,HPV主要结构蛋白L1的表达水平是影响生产效率的主要因素,L2表达质粒的用量过高或过低均不利于假病毒的生产,而报告质粒的用量对假病毒生产效率的影响相对较小.综合比较显示,在该体系中L1和L2表达质粒和报告质粒以合适比例(如1∶1/10∶1/2)进行共转染可获得更为理想的生产效率.本研究同时也在293FT细胞中对磷酸钙转染方法进行了优化,通过磷酸钙与质粒用量的交叉配比试验获得了较优的转染条件.通过优化,建立了更为高效的HPV假病毒大量制备方法,在HPV16、HPV18、HPV6、HPV11假病毒生产中的应用结果显示较原方法可显著提高生产效率.本研究为HPV假病毒中和实验的规模化应用提供了有利条件

Study on the Analysis of PBDEs Residues in Food by Gas Chromatography-Tandem Mass Spectrometry

建立了食品中8种多溴联苯醚(PbdES)残留的气相色谱-串联质谱分析方法,初步解析了PbdES的电子轰击串联质谱(EI MS/MS)图,为各种目标物的准确定性分析提供依据。以bdE-28、bdE-47、bdE-66、bdE-85、bdE-99、bdE-100、bdE-153、bdE-154为研究对象,对EIMS/MS各分析参数进行了优化。用超声提取-酸性硅胶层析柱净化的前处理方法制备样品,当空白样品的加标水平为10.0、25.0μg/kg时,8种PbdES的平均加标回收率为82%--112%,相对标准偏差为3.1%--15%,方法检出限均低于1.5μg/kg;8种PbdES的线性范围为10.0--500μg/kg,相关系数均大于0.994 7。A gas chromatography-tandem mass spectrometric method was developed for the determination of polybrominated diphenyl ethers(PBDEs) residues,including BDE-28,BDE-47,BDE-66,BDE-85,BDE-99,BDE-100,BDE-153 and BDE-154 in food sample.The electron ionization mass spectrometry/mass spectrometry(EI MS/MS) spectra of PBDEs were analyzed.Experimental parameters influencing the behaviour of compounds in the mode of EI MS/MS were investigated and optimized.The sample was extracted with hexane-acetone by ultrasonic assisted extraction and cleaned-up with silica and acidic silica.Under the optimal conditions,the method detection limit(MDL) was lower than 1.5 μg/kg,the average recoveries of 8 PBDEs from spiked samples at two fortification levels of 10.0 and 25.0 μg/kg varied from 82% to 112% with relative standard deviations(RSDs) of 3.1%-15%.The calibration curves of all PBDEs showed good linearities in the range of 10.0-500 μg/kg with correlation coefficients more than 0.994 7.The result indicated that the proposed method had great potential in the analysis of PBDEs residues in food.国家基础科学人才培养基金资助项目(J0630429);山东省科技发展计划资助项目(2009GG20001021-9