焦点解决短期治疗在大学生谈心辅导中的应用

随着时代的发展和大学生呈现出的越来越鲜明的个体特点,探索新型谈心辅导方式的需要日益凸显。注重实效的焦点解决短期治疗技术与谈心辅导相结合,是一种新型谈心辅导方式。这种谈心辅导方式,帮助学生发掘自身资源和优势以解决问题,通过正向开场、问题描述、目标形成、目标可行性方案的确定、反馈五个步骤进行。谈心的过程中不可预设、不可严谈、不可空谈、不可瞎谈

The screening identification and utilization of the anti-angiogenesis plasminagen peptide

人纤溶酶原Kringle5（HumanPlasminogenKringle5，K5）是目前发现的抑制新生血管活性最强的纤溶酶原（Plasminogen）片段，在新生血管性疾病如实体瘤、糖尿病性视网膜增生等多种疾病治疗中具有潜在的应用前景。但是传统的K5存在酵母系统生产工艺复杂、生产成本昂贵、应用剂量大等缺陷，严重制约着K5在临床中的应用。本研究中，我们通过生物信息学方法模拟得到纤溶酶原Kringle中保守的氨基酸序列，合成短肽NH2-NYCRNPD-COOH；在细胞学和组织学水平验证了该多肽对新生血管内皮细胞增殖、迁移以及鸡胚绒毛尿囊膜血管新生的影响；选择大鼠角膜碱烧伤模型，整体水平验证该多肽...Human plasminogen kringle 5 (K5) is found to be the strongest anti-angiogenesis plasminogen fragment. It has potential applications in neovascularization diseases such as solid tumors, proliferative diabetic retinopathy, corneal neovascularization, etc. However, the high costs and traditionally complicated pichia pastoris expression system associated with K5 restricted its clinical application. Us...学位：理学硕士院系专业：医学院药学系_药理学学号：2452007115257

高浓度双乙酰定向驯化获得优良啤酒酵母菌株的研究

在双乙酰浓度为1mg/mL的12°Bx麦芽汁中进行高浓度定向驯化,经涂布抗双乙酰固体选择培养基,从啤酒酿造生产菌株啤酒酵母(Saccharomycessp.)A中富集筛选分离得到一株双乙酰还原速度优于亲株A的新菌株TA4。以12°Bx麦芽汁为培养基用内装300mL麦芽汁的500mL三角瓶10℃下发酵,发酵8d后发酵液双乙酰含量比亲株降低了36.31%,发酵度提高4.5%,且该菌株的絮凝性、发酵速率等特性仍保持了亲株A的优良性状。After domestication with diacetyl (1mg /mL)in25mL 12°Bx wort,a Saccharomyces sp.strain TA4was obtained from orig inal strain A,a strain for beer production.The diacetyl content in the fermented liquid during the fermentation was investig ated in500mL flask with300mL 12°Bx wort at 10℃.After 8days,the diacetyl content in the fermentation broth of TA4was 0.100mg /L,36.31%lower than that of its orig inal strain A(0.157mg /L).The true fermentation deg ree of strain TA4was 4.5%hig her than that of its orig inal strain.Some physical properties were measured.The results showed that flocculation and fermentation rate of strain TA4were the same to the orig inal strain

退火处理对磁控溅射制备LaCoO3薄膜性能的影响

采用磁控溅射法在硅衬底上制备了LaCoO3（LCO）薄膜,研究了退火温度对LCO薄膜组织结构、表面形貌及热电特性的影响,并利用X射线衍射仪、原子力显微镜（AFM）、激光导热仪等对LCO薄膜的晶体结构、表面形貌、热扩散系数等进行测量与表征.结果表明:退火温度对LCO薄膜的结晶度、晶粒尺寸和薄膜表面形貌都有较大影响;退火前后LCO薄膜的热扩散系数都随温度的升高而减小,且变化速率逐渐减缓; LCO薄膜的热扩散系数随退化温度的升高先增大后减小.LCO薄膜经过700℃退火后得到最佳的综合性能,其薄膜表面致密、平整,结晶质量最好,热扩散系数最小,热电性能最好.重庆市教委科技项目(KJ131319

大众化背景下中韩高校招生考试制度比较研究

20世纪50年代以来,世界各国的高等教育纷纷向大众化阶段发展。与此相应,各国对高校招生考试制度进行了必要的改革。韩国是中国的近邻,其招生考试制度的变化发展极具特色。比较分析中韩两国招生考试制度的异同,可以看到,韩国为适应高等教育迅速发展而不断改革和完善高校招生考试制度的一些经验,对我国招生考试制度改革有着重要的借鉴意义

以QQ为平台加强大学生思想政治教育的探索与实践

QQ在大学生中有很高的使用率。它给大学生思想政治教育既带来正面影响,也带来负面影响。以QQ为平台可以加强引导和交流,改进学生自制力的培养和消除师生代沟,缓解和消除学生心理压力,增强学生明辨是非的能力,但利用QQ对学生进行思想政治教育工作时,应严格把关、注重时效,加强监督和引导

BIOSORPTION OF PRECIOUS AND HEAVY METALS*

The biosorption of metals has already attracted a great deal of attention because of its application potential in environment protection and removal or recovery of precious and heavy metals. The research activities about biosorption of precious and heavy metal ions by the biosorbent, the biomass of microorganisms, plants and animals for deriving, was described in this paper. The mechanisms of biosorption were also reviewed. Ref 33国家自然科学基金资助项目 (No .2 98760 2 6)~

Screening research on effective parts of Alisma orientalis( Samuel. ) Juzep. associated with blood glucose decrease in type 2 diabetic mice

目的初步探索泽泻[Alisma orientalis(Samuel.)Juzep.]降低2型糖尿病小鼠血糖的有效部位。方法采用超声提取法获得泽泻乙醇提取物,再分别用石油醚、乙酸乙酯、水饱和正丁醇和水相进行萃取分离,得到的泽泻乙醇提取物及其各溶剂萃取物应用于腹腔注射烟碱、链脲霉素诱导的2型糖尿病小鼠模型。造模成功后,将动物分组,其中空白对照组(未注射链脲霉素和烟碱的小鼠)和2型糖尿病模型组小鼠连续灌胃0.9%Na Cl溶液21 d(灌胃剂量为10μl/g),测试药物组分别连续灌胃泽泻乙醇提取物、泽泻石油醚萃取物、泽泻乙酸乙酯萃取物、泽泻正丁醇萃取物、泽泻水萃取物21 d(各测试药物灌胃高、中、低剂量分别为100μg/g、50μg/g、25μg/g),阳性对照组连续灌胃罗格列酮溶液21 d(灌胃剂量为10μg/g),每组8只小鼠,筛选具有降低2型糖尿病小鼠血糖的泽泻有效部位。用具有降低2型糖尿病小鼠血糖的有效部位处理3T3-L1前脂肪细胞,观察其对前脂肪细胞分化过程的影响。结果与2型糖尿病模型组比较,连续灌胃泽泻乙酸乙酯萃取物21 d(剂量为100μg/g)降低了2型糖尿病小鼠的血糖,改善了口服葡萄糖耐受试验(oral glucose tolerance test,OGTT)过程中的糖耐量(P<0.01)。1 mg/L和10 mg/L的泽泻乙酸乙酯萃取层均能促进前脂肪细胞的分化过程。结论泽泻乙酸乙酯萃取物降低了2型糖尿病小鼠血糖,促进了3T3-L1前脂肪细胞分化,但其具体作用机制尚需深入研究。Objective To explore the effective parts of Alisma orientalis( Samuel.) Juzep. associated with blood glucose decrease in type 2 diabetic mice.Methods Ethanol extracts from A. orientalis were obtained by ultrasonic extraction. Then,the ethanol extract was extracted and separated with petroleum ether,ethyl acetate,water saturated n-butanol and water respectively. After decompressing concentration,these solvent extractions were applied to type 2 diabetic mice model induced by intraperitoneal injection of streptozotocin and nicotine. After successful modeling,the mice were divided into the blank control group( without injection of streptozotocin and nicotine),model group of type 2 diabetes mellitus,positive control group and test drug groups,8 in each group. The blank control group and model group were treated with 0. 9% Na Cl at the dose of 10μl / g,the test drug groups were treated with corresponding extracts from different solvent extractions described above at the high-,mid-,low-dose of 100,50,25 μg / g respectively. The positive control group was treated with rosiglitazone at the dose of10μg / g. The treatment course was 21 days for intragastric administration. The effective parts of Alisma orientalis( Samuel.) Juzep. associated with blood glucose decrease in type 2 diabetic mice were screened and then the effect of the screened effective part on the differentiation of 3T3-L1 pre-adipocyte was observed.Results Compared with the model group,the blood glucose was decreased after the intragastric administration with ethyl acetate extract of A. orientalis at dose of100μg / g for 21 days and the glucose tolerance during the oral glucose tolerance test( OGTT) was improved( P < 0. 01). The differentiation of pre-adipocyte was promoted when the ethyl acetate extract of A. orientalis at the concentration of 1 mg / L and 10 mg / L. Conclusion The ethyl acetate extract of A. orientalis reduced the blood glucose in type 2 diabetic mice and promoted the differentiation of 3T3-L1 pre-adipocytes,but the specific mechanism still need to be further studied.福建省科技厅自然科学基金青年创新项目;; 厦门市卫计委资助项目(2014D008),厦门市卫计委资助项目(2015-ZQN-JC-45);; 福建省卫生系统中青年骨干人才培养项目基础项目;; 福建省科技厅自然科学基金(2015J01065);; 福建省教育厅中青年教师教育科研项目杰青项目(JA14421),福建省教育厅中青年教师教育科研项目(JA13425);; 厦门市科技局科技计划高校创新项目(3502Z20143026),厦门市科技局科技计划项目(3502Z20144031);; 厦门医学高等专科学校科研基金(Z2013-12,Z2013-25

厦门市思明区老年人对医养结合养老模式的认知和参与意愿现状及其影响因素

目的:调查厦门市思明区老年人对医养结合养老模式的认知及参与意愿,分析其影响因素,为推进医养结合养老工作的发展提供参考。方法:采用方便抽样方法,对户籍在厦门市且居住在思明区的60周岁及以上的114名老年人进行问卷调查。结果:目前厦门市思明区老年人对医养结合养老模式的认知处于较低水平,参与意愿偏低。年龄、文化程度、子女、了解程度是影响老年人医养结合养老模式参与意愿的因素,其中文化程度、了解程度是影响参与意愿的主要因素。结论:养老机构应采取形式多样的宣传教育,提高老年人对医养结合养老模式的了解程度及参与意愿,推进医养结合养老产业的发展

宽带激光熔覆送粉喷嘴的结构设计与粉末流场研究

设计一种新型的送粉喷嘴结构,适用于宽带激光熔覆和增材制造。该结构使用多通道出口有效降低粉末流在扫描方向的发散角,通过添加内部整流圆柱调整粉末流在垂直扫描方向上的浓度分布。采用离散相模型,研究该喷嘴结构输出的粉末流场。优化分流圆柱的尺寸,使该送粉喷嘴能够输出浓度分布均匀的宽带粉末流,并沉积出厚度均匀的宽带涂层