香豆素-半花菁-苯硼酸对唾液酸的识别与光学传感

以共轭连接的香豆素和半花菁为信号报告基团,以苯硼酸为识别基团,设计合成了一种对唾液酸具有选择性识别的比色型荧光传感分子,命名为CHB,并采用吸收和荧光光谱方法研究其对唾液酸(N-乙酰神经氨酸)及其他糖类分子(如葡萄糖、蔗糖和麦芽三糖等)的响应.结果表明,CHB仅对N-乙酰神经氨酸表现出显著的吸收和荧光光谱响应,并通过高分辨质谱研究证实二者以1∶1计量比通过共价作用结合形成复合物.N-乙酰神经氨酸与苯硼酸的共价作用和静电作用共同影响了香豆素-半花菁共轭体系的分子内电荷转移,从而引起显著的溶液颜色变化和高选择性的光谱响应,因此CHB可用于N-乙酰神经氨酸的可视化检测.国家自然科学基金(21435003,91427304,21521004,J1310024

重组人瘦素的基因构建、表达及活性鉴定

根据NCBI中公布的人瘦素cDNA序列,设计并合成了6个89bp左右的DNA小片段,经重叠延伸PCR扩增获得464bp的rhLep的完整基因片段。构建pET22b(+)/rhLep表达载体,转化大肠杆菌BL21(DE3),IPTG诱导表达。目的蛋白以包涵体的形式表达,表达量占菌体总蛋白的50%以上。表达产物用Ni2+亲和层析柱纯化,SDS-PAGE结果表明,含6×His标签的目的蛋白的相对分子量约16kD。MTT比色法实验显示,当低剂量(10~30ng/mL)时,rhLep具有促进内皮细胞生长的作用;在高剂量(50~225ng/mL)时,rhLep具有杀伤人内皮细胞的活性。其最大杀伤率达到98.8%。吖啶橙荧光染色观察到高剂量rhLep对人内皮细胞有明显的凋亡作用。以上工作为进一步了解瘦素的体内体外生物活性奠定了基础

Resonance Light Scattering Spectra of Mercury Reineche Salt System in a Microemulsion Medium and Its Analytical Properties

研究了微乳液的增稳作用对汞- 雷氏盐乳浊体系的共振光散射（rlS） 光谱， 该体系的rlS光谱强度与浓度成正比， 峰值波长位于400nM 处. 在最佳的测定条件下， 线性范围为0 ~19-2μg/M l， 相关系数r＝ 0-9998 ， 检测限为0-023μg/Ml.This work briefly states the spectra of resonance light scattering(RLS) of mercury reineche salt system in a microemulsion medium.The spectral characteristics,the effect factors and optimum conditions of the reaction have been investigated.The quantitative relationship of the intensities of RLS to the concentration of mercury(Ⅱ) were established

长汀县赢坪史前遗址发掘简报

2012年福建博物院对赢坪两处遗址进行了抢救性考古发掘,获得了一批包括石器、陶器、铜器、石范等内涵丰富的文化遗物,这批遗物可进一步了解闽西地区的史前文化发展序列及文化面貌,并且对于多地区文化间的交流研究也有一定的作用

The study of plant community character in Alice Lake Wetland

艾里克湖是克拉玛依唯一的天然湖泊，近年来由于人口增加和过量地开发利用水土和生物资源，致使湖泊面积不断减小，动植物种类减少，加剧了荒漠化的发展，导致区域环境恶化。本文应用双向指示种分类法(TWINSPAN)和典范对应分析法(CCA)，对艾里克湖湿地及其周边植物群落与环境因子的关系进行了研究，确定了影响该研究区域植被分布主要环境因子是地下水位埋深和土壤有机质的含量，结果表明，该研究区域主要植被类型中共有植物18种，隶属10科，17个属。其中，多数植物种类在样方中出现的频次较低，说明该地区植物物种丰富度较低。TWINSPAN结果表明，该地区植物可以分9个种类组，与实际调查结果一致。同时对艾里克湖湿地植物群落特征指数与土壤环境因子的关系进行研究，结果表明，研究区的主要植物分为4个种类组，其中胡杨、毛柽柳、芦苇和西伯利亚泡泡刺是最常见的种类。该区25个样地分为3个主要类型组，组1包括6个样地，组2包括18个样地，组3包括1个样地，分别代表不同土壤有机质、全氮、全磷含量的3种生境，阐明了有机质、全氮、全磷的含量对植被分布的影响。CCA排序结果同TWINSPAN分类结果基本一致，反映出植物群落分布格局随环境因子梯度变化的趋势。Alice Lake is the only crude lake in the Kelamayi area. Recently, the population increased and excessive exploitated water, soil and biology resources have caused duratively shink in the area of lake and the number of species plant and animal decreased. More and more argent problem lies ahead of us, that is the ecological enviroments of lakes become deteriorated and the trend of land desertification more severity.Quantitative classification (TWINSPAN) and ordination (CCA) methods were used to study the relationship between plant communities and environmental factors wetland and surrounding lands in the Alice Lake of arid land. The results showed that 18 plant species were identified, which belong to 17 genera in 10 families of this study, most of which have low occurrence frequency in quadrats. The 18 plant species could be also divided into 9 ecological species groups using TWINSPAN analysis. The distribution patterns of plant species were most strongly associated with ground water, soil organism.This ways were used to study the relationship between plant community character index and soil factors in the Alice Lake wetland too. The results showed that the major plant species in the study region were 4 ecological species groups, Populus euphratica, Tamarix hispida, Phragmites australis and Nitraria sibirica are the most common species in this study area. The analysis by TWINSPAN showed that 25 plots could be classified into 3 groups. Group 1 contains 6 sites, Group 2 contains 18 sites, and Group 3 contains 1 site. They express three habitats of different organic matter content, total nitrogen content, and total phosphorus content. CCA results were consistent with TWINSPAN results. The distribution patterns of plant species were most strongly associated with soil organism, total nitrogen, and total phosphorus. To a lesser extent, distribution of species was associated with soil pH value, conductivity and salinity. The major environment variables are soil organism, total nitrogen, and total phosphorus

Motion of an autonomous underwater vehicle-manipulator system based on range limit

对自治水下机器人搭载的四功能水下电动机械手进行了简要描述。考虑到自治水下机器人机械手系统的运动学冗余,将关节限位算法用于系统逆运动学求解,避免载体大幅度姿态变化。利用Matlab仿真表明该算法在解决系统冗余的同时有效的限制了关节位移

Recognition of Anion by Dual Fluorescent Sensor Based on the Intramolecular Charge Transfer in p-Dimethylaminobenzoylhydrazine

In t ramo lecu lar charge t ran sfer ( ICT ) dual f luo rescence of p 2dim ethylam ino2 benzoylhydrazine (DMABH ) in aceton it rile w as found to show a h igh ly select ive respon se to HSO -4 over several other an ions. The inten sity rat io of the CT to L E em ission and the to tal f luo rescence intensity of DMABH decreased with increasing bisulfate concent ration. While the dual f luo rescence respon se w as very sim ilar to that of DMABA at a low HSO4 concen t rat ion, the CT in ten sity decreased drast ically and the L E in ten sity rem ained almo st con stan t, how ever, the difference w as ob served at a h igh HSO -4 concen t rat ion, in w h ich bo th the CT and L E in ten sit ies of DMABH decreased. A different recogn it ion m echan ism f rom that of DMABA w as suggested fo r DMABH in w h ich hydrogen bonding and p ro ton izat ion w ere the recogn it ion in teract ion s at low and high HSO4 concen t rations,respectively.国家自然科学基金 (批准号 :2 0 175 0 2 0 );福建省自然科学基金重点课题 (批准号 :D0 2 2 0 0 0 1);教育部优秀青年教师奖 励计划 ( 2 0 0 1年 )和德国大众基金 (批准号 :I/770 72 )资助

艾里克湖湿地植物群落特征指数与土壤因子的关系

应用双向指示种分类法(TWINSPAN)和典范对应分析法(CCA),对干旱区艾里克湖湿地植物群落特征指数与土壤环境因子的关系进行研究。结果表明,研究区的29种主要植物,隶属12科27属,分为4个种类组,大多数物种丰富度较低,其中胡杨、毛柽柳、芦苇和西伯利亚泡泡刺是最常见的种类。TWINSPAN分析表明,该区25个样地分为3个主要类型组,组1包括6个样地,组2包括18个样地,组3包括1个样地,分别代表不同土壤有机质、全氮、全磷含量的3种生境。CCA排序结果同TWINSPAN分类结果基本一致,反映出植物群落分布格局随土壤因子变化的趋势,确定了影响该区植物群落盖度的土壤因子为有机质、全氮、全磷

离子液体的制备及应用

离子液体由于具有独特的物理化学性质而成为一种新型的绿色溶剂,近年来成为国际上研究的前沿和热点。它为开发新型绿色工艺、实现传统重污染、高能耗工业过程的升级换代提供了新机遇。本文介绍了离子液体的合成与制备方法,离子液体在CO2捕集分离和转化利用、电解/电镀铝、SO2吸收、废水处理以及废旧塑料降解循环利用中的应用,最后展望了离子液体的发展前景

白杨河——艾里克湖湿地及周边植物群落与环境因子的关系

应用数量分类和排序方法,对新疆白杨河-艾里克湖湿地植被进行了多元统计分析。结果表明,该研究区域主要植被类型中共有植物18种,隶属10科,17个属。其中,多数植物种类在样方中出现的频次较低,说明该地区植物物种丰富度较低。TWINSPAN结果表明,该地区植物可以分9个种类组,与实际调查结果一致。CCA排序结果同TWINSPAN分类结果基本一致,反映出植物群落分布格局随环境因子梯度变化的趋势。分析了不同环境因子的关系,并从定量的角度,以CCA排序图直观的揭示了决定艾里克湖湿地植物群落分布的环境因子主要是地下水位、土壤有机质,其回归模型分别为:X_1=-0．6037L+0．6824(R~2=0．6024);X_2=0．74530M+0．1142(R~2 =0．6004)