143 research outputs found

    Property Right Trade,Institution Evolution and the M&A of State-Owned Companies

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    企业并购本质上是产权交易。产权交易的前提是产权的清晰界定。作为产权交易的一种重要方式,企业并购的前提也必然是产权的清晰界定。另外,企业制度安排对企业并购活动的顺利进行产生重要的影响。阻碍中国国有企业并购活动有效开展的主要是产权方面及企业制度上的问题。解决这些问题的关键是:一方面,我们要不断完善国有资产的管理监督体制,加强国有产权运营过程中的监督力度;另一方面,建立和完善现代企业制度与企业法人产权制度,明确国有产权实现过程中各权利主体的权、责、利关系问题。The M&A of enterprises is in fact the exchange of property rights.The premise of property rights dealing is the clear definition of property rights.As a form of property rights dealing,the premise of M&A is certainly the clear definition of property rights.The system arrangement of enterprises has an important impact on the M&A.The problems that constrains the M&A of China's state-owned companies is mainly the problems on property rights and some on the system,so,we must perfect the management system of state-owned assets and pay attention to the operation of property rights.On the other hand,it is necessary to improve the property rights institution of company legal person and definite the relation of rights,duty and benefit of every principal part in the process of property rights realization

    金属矿千米深井高应力特性岩爆发生规律与防治措施研究

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    针对大红山铁矿进入千米深井开采后,在巷道、竖井和大断面硐室的基建开挖过 程中发生的多次不同程度的岩爆动力地压现象等严重的地压灾害问题,首先对千米深部 采区的高地应力特性进行了研究,得到了其深部高地应力处于超过亚临界深度的状态,但 还没有达到临界深度的总体评价结果;其次采用模糊综合评价法获得了不同岩性在不同 埋深的岩爆倾向性,得到了深部采区岩体总体上具有中等岩爆倾向性,在最深-40 m 水 平的局部,大理岩、片岩和磁铁矿的岩爆倾向性趋于强烈的结论;其次全面统计了基建过 程中发生的岩爆案例,获得了岩爆发生与埋深、地应力、岩性等的关系与规律,得到了岩爆 数量随埋深的增加呈指数关系增加的规律;最后提出了易爆岩层巷道与硐室的岩爆动力 地压灾害的防护措施.国家自然科学基金项目(51674218)国家重点研发计划项目(016YFC0600702)

    Effects of mixed-culture densities of Spartina alterniflora on eco-physiological characteristics of Kandelia candel seedling

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    按照不同密度将互花米草与秋茄进行混种,对比研究胁迫条件下秋茄幼苗生长、光合特性及其渗透调节物质变化规律。结果表明,低密度互花米草促进秋茄的茎长以及各部分生物量,高密度则起抑制作用。随着互花米草密度的增大,秋茄幼苗叶片光合速率、气孔导度、蒸腾速率、水分利用率和蛋白含量均下降;相反,胞间CO2浓度、可溶性糖、淀粉、脯氨酸含量却上升,这些变化有利于对抗互花米草带来的不利影响。 【英文摘要】 Kandelia candel seedlings were mix-cultured with Spartina alterniflora of different densities for 120 days,and their growth,photosynthetic characteristics as well as osmotic adjustment law were studied.The results showed that the stem height and biomass of K.candel seedlings were enhanced with low density S.alterniflora and inhabited with high density ones.With the density of S.alterniflora going up,Pn、Gs、Tr、WUE and protein content in K.candel seedlings decreased respectively.On the contrary,the content of ...国家自然科学基金资助项目(30670317,30271065);; 福建省自然科学基金资助项目(D0210001

    Studies on Light-Scattering Nephelometic Determination of Sulfate Radical with Polyvinyl Alcohol-Barium Sulphate System

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    对聚乙烯醇—硫酸钡乳浊体系的共振光散射光谱、二级光散射光谱与反二级光散射光谱的光谱特性进行研究, 并开展了用此体系测定环境水样中硫酸根的应用研究, 3 种散射光谱法在0 ~0-24Mg/Ml的硫酸根浓度范围内的标准曲线均呈良好线性关系.The spectra of resonance light scattering(RLS),double scattering(DS) and anti-double scattering(ADS) for polyvinyl alcohol-barium sulphate system have been studied by using an ordinary spectrofluorophotometer.RLS,DS and ADS have been applied to the determination of sulfate radical in environmental water sample.The results are satisfactory

    Resonance Light Scattering Spectra of Mercury Reineche Salt System in a Microemulsion Medium and Its Analytical Properties

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    研究了微乳液的增稳作用对汞- 雷氏盐乳浊体系的共振光散射(rlS) 光谱, 该体系的rlS光谱强度与浓度成正比, 峰值波长位于400nM 处. 在最佳的测定条件下, 线性范围为0 ~19-2μg/M l, 相关系数r= 0-9998 , 检测限为0-023μg/Ml.This work briefly states the spectra of resonance light scattering(RLS) of mercury reineche salt system in a microemulsion medium.The spectral characteristics,the effect factors and optimum conditions of the reaction have been investigated.The quantitative relationship of the intensities of RLS to the concentration of mercury(Ⅱ) were established

    Phytoplankton community structures revealed by pigment signatures in Norwegian and Greenland Seas in summer 2012

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    对2012年中国第5次北极科学考察期间的挪威海和格陵兰海两个断面的光合色素进行了高效液相色谱(HPlC)分级分析,通过藻类色素化学分类分析软件(CHEMTAX)获得了不同浮游植物类群对叶绿素A的贡献,进而得到该海域表层和次表层(30 M)的浮游植物群落结构。结果表明:表层总叶绿素A的浓度为23.59 ng/l,低于次表层的30.38 ng/l,其中浮游植物根据粒径划分对总叶绿素A的贡献由高到低依次是微型浮游植物、小型浮游植物和微微型浮游植物。该海域同时存在葱绿叶绿素(PrASInO)、墨角藻黄素(fuCO)、别藻黄素(AllO)、多甲藻素(PErId)、玉米黄素(zEA)、19-丁墨甲藻黄素(19'bf)和19-六已墨甲藻黄素(19'Hf)等色素,其浓度和分布与温盐和营养盐等环境因子存在一定的相关性。不同粒径浮游植物色素组成显示,微微型浮游植物群落中以S型定鞭藻(28%)、n型定鞭藻(21%)、硅藻(18%)和青绿藻(12%)占优;微型浮游植物群落的优势类群为S型定鞭藻(53%)、n型定鞭藻(20%)和硅藻(12%);而小型浮游植物群落主要为硅藻(63%)和甲藻(17%)。Composition of phytoplankton controlled not only the formation of the Food Chain,but also the efficiency of energy transfer.At the same time,phytoplankton influenced the distribution of nutrient and the sedimentation of organic particulate matter.So study of phytoplankton community structures plays an important role in understanding the ecological function of phytoplankton.Photosynthetic pigments needed by photosynthesis,are very good biomarkers.They can be used to research and characterize phytoplankton community structure.Norwegian and Greenland Seas which are influenced by the Arctic water with low temperature and salinity and Atlantic water with high temperature and salinity.Understanding of correlation between environmental factors and phytoplankton community structure,is important for understanding the community structure of the marine organisms and their ecological function.During the 5thChinese Arctic Research Expedition in summer 2012,we analyze the photosynthetic pigments fromNorwegian and Greenland Seas by HPLC.Contributions of different phytoplankton assemblages to Chlorophyll a were obtained by Chemical Taxonomy( CHEMTAX),and thus resulted in composition of the phytoplankton community structure at the surface and subsurface water( about 30 m depth).The result showed that average concentration of Chlorophyll a at surface water was 23.59 ng / L,lower than that at upper water( 30.38 ng / L).The contributions of phytoplankton to Chlorophyll a were nano-> micro-> pico-one.Prasinoxanthin,Fucoxanthin,Alloxanthoxyletin,Peridinin,Zeaxanthin,19'-but-fucoxanthin,19-hexanoyloxyfucoxanthin were observed in the waters.Their distributions were relative to environmental factors,including Nutrients,temperature and salinity.These photosynthetic pigments showed negative correlations with temperature and salinity.Whereas they showed positive relations with nutrients,including NO-2,NO-3,SiO3 2-and PO3-4.The correlations of different phytoplankton and environmental factors were different.Diatoms had a very significant positive correlation with SiO3 2-,but had no correlation with PO3-4,NO-2and NO-3; Haptophytes-N had significant positive correlations with PO3-4,NO-2and NO-3; Haptophytes-S also had a positive correlation with PO3-4,but had no correlation with NO-2and NO-3.The maximum values of these photosynthetic pigments,except for Zea and Fuco,existed in the subsurface of the section BB.The distribution and concentration of photosynthetic pigments could explain the distribution and abundance of the phytoplankton to some extent.Not only because the pigments are complex and multiple,but also because there are inevitable errors in the testing and sampling,we cant determine the existence and abundance of the phytoplankton.So we use the CHEMTAX to obtain the contributions of different phytoplankton assemblages to Chlorophyll a,and thus resulted in composition of phytoplankton community structure.The composition of phytoplankton showed that Haptophytes-S( 28%), Haptophytes-N( 21%),Diatoms( 18%),and Prasinophytes( 12%) were main assemblages in the picoplankton; the main composition of nanophytoplankton were Haptophytes-S( 53%),Haptophytes-N( 20%),and Diatoms( 12%); meanwhile, Diatoms( 63%) dominated the microphytoplankton,followed by Dinoflagellates( 17%).Haptophytes-S,Haptophytes-N and Diatoms were dominant species in the surface and subsurface of section BB and AT belonging to the Norwegian and Greenland Sea.If Fucoxanthins and Diatomss existence in picophytoplankton could explain the tendency of phytoplankton to micro and miniaturization,these still need to be more sufficient evidence.国家自然科学基金(41076130;41206189); 南北极环境综合考察与评价专项(CHINARE2011-2015

    L-茶氨酸局部给药抑制皮肤神经末梢的异常生长缓解小鼠银屑病瘙痒

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    目的观察L-茶氨酸是否在咪喹莫特(IMQ)诱导的银屑病小鼠中具有止痒作用并初步探讨机制。方法将IMQ软膏局部应用于小鼠背部皮肤建立银屑病模型;L-茶氨酸经口服(10 mg/kg∙d)或病损皮肤局部涂抹(50、125、250 mmol/L,2次/d)进行防治处理。采用抓挠等自发性行为学测试检测治疗效果;并运用免疫荧光染色(IF)、Western Blot(WB)及原代背根神经节(DRG)神经元培养技术探讨相关机制。结果口服L-茶氨酸对IMQ诱导的瘙痒无明显作用,但病损皮肤局部应用明显改善银屑病模型小鼠皮肤不舒适感诱发的舔或咬及畏缩等自发性行为,并呈浓度依赖性。IF和WB结果显示,L-茶氨酸不仅能抑制模型小鼠皮肤TNF-α过表达,还能阻止β3-tubulin神经纤维的异常生长和瘙痒相关分子瞬态受体电位香草酸1(TRPV1)的上调。体外实验进一步证实L-茶氨酸能浓度依赖性地直接抑制IMQ引起的CGRP+、β3-tubulin+神经突起异常生长。结论局部应用L-茶氨酸通过抑制皮肤神经纤维的异常生长缓解小鼠银屑病模型的皮肤瘙痒不适感。因此,L-茶氨酸局部给药可成为治疗银屑病慢性瘙痒的一种潜在方法

    改良的 Trizol 法提取小鼠皮肤 RNA 技术

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    【目的】由于皮肤组织韧性强且存在大量RNA酶,采用经典Trizol法提取的RNA存在易降解质量低等不足。因此,本研究旨在通过改良的Trizol法实现高质量的皮肤RNA提取。【方法】采用不同处理方式(Tri:Trizol包埋;Pro:RNA样本保护液包埋;Cry:先冷冻液氮再冷冻-80℃冰箱;LNG:液氮研磨;Cut:采用剪刀剪碎组织)提取的小鼠正常皮肤RNA为实验组;脊髓组织作为参照组,并以咪喹莫特(IMQ)诱导的小鼠银屑病模型皮肤组织作为保真性验证组。我们采用紫外分光光度法、琼脂凝胶电泳法、定量逆转录PCR法(qRT-PCR)等方法来检测经典Trizol法(1-Tri,Nor)和改良Trizol法(2-Tri,LNG-Tri,Tri-Cut,Pro)提取的RNA浓度、纯度和完整性以及IL-1β mRNA表达的差异性情况。【结果】①与脊髓组织相比,经典Trizol法(1-Tri)获得的正常皮肤组织总RNA量要低,DNA污染和5SRNA条带明显,IL-1β mRNA相对表达偏高,说明经典Trizol法在皮肤组织中提取RNA存在局限性,需要进行改良。②在不同小鼠皮肤样本处理组中,改良的2-Tri法和LNG-Tri法获得的RNA浓度更高,同时RNA降解、DNA污染更低,IL-1β mRNA表达更接近正常水平。更重要的是,在小鼠银屑病模型中,2-Tri法提取的RNA样本能真实反映出正常皮肤与银屑病变皮肤之间IL-1β mRNA的变化趋势。【结论】改良的2-Tri或LNG-Tri法分离的总RNA质量好,能可靠地反映生理或病理条件下的mRNA表达模式

    Analysis of buckling deformation of positive plates during formation at low temperature

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    [中文文摘]在低温条件蓄电池生产中,极板化成时经常产生正极板弯曲的现象。对此进行了研究,发现在低温下,电化学反应形成了致密的结晶,很难形成颗粒状的活性物质和分布均匀的孔隙,产生的应力无法释放;低温下化成的极板内部和极板外部的成分差异很大,也是产生应力的因素之一,这些应力导致了极板的变形。[英文文摘]During battery production at low temperature, buckling phenomenon of positive plates often appeared due to the formation of plates. Research showed that electrochemical reaction formed the dense crystals at low temperature. It is difficult to form the granular active materials and uniform distributed porosity, so that the resulted stress could not be released. During formation at low temperature, the difference of components between the internal and external plates was remarkable. This was also one of the factors of resulting in stress which lead to the deformation of plates

    Effect of salinity on microbial densities of soil in the dilution plate technique applied in mangrove areas

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    作者简介:张瑜斌(1970~) ,男,湖南郴州人,博士,副教授,主要从事海洋微生物学与海洋生态学研究. E2mail: zhangyb@gdou. edu. cn 通讯作者Corresponding author. E2mail: linpeng@jingxian. xmu. edu. cn[中文文摘]在使用稀释平板法分离潮间带红树林及其对照光滩土壤微生物以及计数时,多数情况下使用陈海水制作培养基和稀释水,很少考虑培养基和稀释水的盐度对最终计数结果的影响。使用稀释平板法研究了盐度对福建九龙江口红树林区与深圳福田红树林保护区土壤微生物平板计数的影响,结果表明培养基与稀释水盐度对微生物数量有明显的影响。统计分析显示细菌的海水稀释效果优于淡水,而放线菌与真菌则刚好相反(P<0.05,一个例外)。海水不适合配制红树林区土壤微生物平板计数的培养基,从0~35,高盐度的平板培养基会降低微生物的数量,尤其是放线菌的数量,尽管培养基的盐度对真菌影响无规律,但细菌数量在低盐度时比在高盐度和不加氯化钠时要多。根据盐度效应,提出了稀释平板技术应用于潮间带的红树林及其相应光滩时的优化方法,认为细菌应该用海水作无菌稀释水,而放线菌和真菌则应用淡水作稀释水;包括光滩在内的红树林区土壤微生物分离与计数的培养基宜控制较低盐度范围。[英文文摘]When the soilmicrobial densities are determined in mangroves and correspondingmudflat at the same tidal level by the dilution p late method, the agarmedia and dilution water are generallymade up of aged seawater in most cases, and effects of salinity in agar media and dilution water on the enumeration of microbes is seldom taken into consideration. The effects of salinity on soil microbial counting from the samples in mangrove areas in Jiulongjiang Estuary of Fujian, and Futian Mangrove Nature Reserve of Shenzhen, China, were tested by dilution p late technique. The results showed that the soil microbial densities in mangroves and mudflat were significantly influenced by the salinity of dilution water and agarmedia. For the bacteria, the seawater served as sterilized dilution water was significantly ( P < 0. 05) more benefic to the enumeration on the p lates than the freshwater, but in reverse for the actinomycetes and fungi. The increasing salinity of media within 35 significantly decreased microbial colonies on the p lates, especially for the actinomycetes, in sp ite of the fact that the effect of salinity ofmedia on fungal numberswas not indefinite. The bacterial colonieswere more abundant on the agar p lates with low salinity than with high salinity or without any NaCl. It was p roposed that some methodological imp rovements were needed when the dilution p late technique was app lied to microbial counting in the samp les of mangrove forest and mudflat at the same tidal level in inter2tidal zone. The sterilized dilution water should be p repared with seawater for the bacteria, but with freshwater or low saline water for the actinomycetes and fungi. The salinity of agarmedia should be low for the microbial isolation and enumeration of soil samples from the mangrove areas including mudflats.国家自然科学基金资助项目(30270272
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