Transformed Rice with Salt Tolerance-related Genes of Bruguiera sexangula by Agrobacterium Meditation

运用农杆菌介导法将红树林耐盐相关基因mangrin转入粳稻品种‘日本晴’中,通过GUS基因检测愈伤组织转化率,确定农杆菌菌液浓度OD600为0.5,浸染时间30min,共培养时间3d为最佳转化体系;经潮霉菌筛选,获得抗性再生植株。通过PCR扩增检测、Southern blot分析和GUS基因活性检测,结果表明,mangrin基因整合到再生水稻的染色体DNA上。耐盐性测定结果表明,转基因植株在200mmol/L NaCl胁迫下,成活率保持在83.3%,株高增长20%~40%,mangrin基因能提高转基因水稻对盐胁迫的抗性。Salt tolerance-related genes in Bruguiera sexangula were transformed into Oryza sativa subsp.japonica cv.Nipponbare,a keng-rice variety.The optimal system of the transformation was found by detecting the callus-transformation rate with GUS gene to be the one with the agro-bacterium concentration set at OD_ 600 0.5,the infection time set at 30 minutes and the culture time set at 3 days and then the regeneration plantlets were obtained by Hyg screening.mangrin gene was integrated in the DNA of the regeneration plantlets by PCR amplification,Southern blotting and GUS gene activity detection.The testing of the salt tolerance indicated that under the stress resulting from 200 mmol/L NaCl the transgenic plants maintained a survival rate of 83.3% and increased their heights by 20%~40% and thus mangrin gene could raise the resistance of the transformed rice to salt stress.中国科学院知识创新工程重要方向项目(KZCX3-SW-444);; 教育部重点项目(01102

肼在金属表面上分解机理的理论研究

键指数归一-二次指数势(Unity Bond Index-Quadratic Exponential Potential,UBI-QEP)法被用于研究肼在Fe,Ru,Pt和Cu表面上的分解机理.研究结果表明,肼在金属上优先发生N—N键断裂,金属活性顺序是Ru~Fe>Pt>Cu,但不同金属上呈现出不同的产物选择性.在Fe,Ru上产物主要为N2和H2,其通过N2Hx物种形成的可能性较低,金属活性顺序为Ru>Fe;而在Cu,Pt上最终产物为NH3,N2和H2,其中H2和N2的形成可能部分源于中间体物种N2H的转化,金属的活性顺序为Pt>Cu

肼在金属表面上分解机理的理论研究

键指数归一-二次指数势(Unity Bond Index-Quadratic Exponential Potential,UBI-QEP)法被用于研究肼在Fe,Ru,Pt和Cu表面上的分解机理.研究结果表明,肼在金属上优先发生N—N键断裂,金属活性顺序是Ru~Fe>Pt>Cu,但不同金属上呈现出不同的产物选择性.在Fe,Ru上产物主要为N2和H2,其通过N2Hx物种形成的可能性较低,金属活性顺序为Ru>Fe;而在Cu,Pt上最终产物为NH3,N2和H2,其中H2和N2的形成可能部分源于中间体物种N2H的转化,金属的活性顺序为Pt>Cu

能量分解法在羰基配合物成键本质研究中的应用

通讯联系人, E-mail： [email protected]借助量子化学能量分解法的思想,以对Cr(CO)6及TMq(CO)6(TMq=Hf2-,Ta-,W,Re+,Os2+,Ir3+)中金属-羰基的成键中能量贡献的分解分析为例,对金属-羰基成键中不同类型的相互作用和成键本质进行了分析和总结,并与人们传统的认识进行了比较。国家基础科学人才培养基金(J0630429

Pathways between superoxide and peroxide species on small La-O clusters

采用密度泛函理论方法考察了lA-O团簇上超氧物种与过氧物种间转化的连接途径.单重态下,团簇上单个超氧物种可通过一系列臭氧物种转化为过氧物种,且转化能垒较高;三重态下,单个超氧物种则并无与过氧物种间连接的途径.然而,lA-O团簇上两超氧物种间的相互作用及其转化也具单重态和三重态两条途径.三重态下,超氧物种可很容易地转化为过氧物种(O2+O2O22+O2),超氧物种与过氧物种处于快速的交换状态之中;单重态下,超氧物种转化为过氧物种则需较高的活化能垒,表明在单重态下这些氧物种具有较高的稳定性.Density functional theory calculations were used to investigate the connection between superoxide and peroxide species on La-O clusters.In the singlet state,a superoxide species can transition into a peroxide species by moving through a substantial energy barrier via a series of ozonides.In the triplet state,there is no connection between the two species,although there are two paths(singlet and triplet) that allow the interaction and subsequent transformation of two superoxide molecules on a La-O cluster.The superoxide species readily transitions to a peroxide species through a triplet pathway(O2–+ O2– O22– + O2),in which the superoxide species undergoes rapid exchange with the peroxide.In the singlet path,however,the superoxide species must move through a pronounced energy barrier to change into a peroxide species,demonstrating that these oxygen species are high- ly stable in the singlet state.supportedbytheNationalBasicResearchProgramofChina(973Program;2010CB732303); theNationalNaturalScienceFoundationofChina(21033006;21373169;20373054); theProgramforChangjiangScholarsandInnovativeResearchTeamintheUniversity(IRT1036)~

Monte Carlo法模拟CO在Fe(100)表面的升温脱附

以类桥位的模型为基础，采用ＭｏｎｔｅＣａｒｌｏ算法，结合键级守恒－Ｍｏｒｓｅ势方法（ＢＯＣ－ＭＰ），模拟了ＣＯ在Ｆｅ（１００）表面上平躺式吸附，考虑了金属与吸附质（Ｍ—Ａ）、吸附质与吸附质（Ａ—Ａ）之间的相互作用，以研究小分子在金属表面上的ＴＰＤ谱图，分析了ＣＯ的解离过程和脱附过程对ＴＰＤ谱图的影响，结果表明，理论模拟与实验相

Research on Salt Tolerance of Rice Transformed by mangrin Gene

以转MAngrIn基因水稻的阳性植株(11#,59#,86#)和阴性植株为材料,在0,100,150,200 MMOl/l的nACl溶液中经盐胁迫7 d后,测定叶片相对含水量、质膜透性、超氧化物歧化酶(SOd)活性和脯氨酸含量4项耐盐指标。结果表明:盐胁迫下,转基因植株的叶片相对含水量、脯氨酸含量、SOd活性明显高于阴性植株,叶片电导率则低于阴性植株。对转基因植株和阴性植株的耐盐性综合评定结果是11#>59#>86#>阴性植株。Plants transformed by mangrin gene(11#,59#,86#) and negatives were tested using several salt tolerance indices,including related water content,cell membrane permeability,SOD activity and proline content,which were conducted under the NaCl concentration of 0,100,150,200 mmol/L after 7 days.The results indicated that the transgenic rice plants accumulated more RWC,proline content and SOD activity than ck under salt stress conditions,yet cell membrane permeability decreased less than ck.According to physiological target in synthetically evaluation,the result is 11#> 59#> 86#> Negatives.福建省青年人才基金项目(2006F3022);福建省自然科学基金项目(2007J0250

沙格列汀治疗2型糖尿病病人致心衰风险的荟萃分析

目的:系统评价2型糖尿病病人用沙格列汀治疗与心衰风险的相关性。方法:检索中文科技期刊全文数据库(维普)、万方数据-数字化期刊群、中国期刊全文数据库(中国知网)、PubMed和Cochrane library建库至2017年12月的相关文献。按Cochrane系统评价法筛选沙格列汀致心衰的所有随机、对照临床试验(RCT),将纳入的文献进行数据提取和质量评价后,应用RevMan 5.3软件进行meta分析。结果:共纳入11篇文献,meta分析结果显示:沙格列汀致心衰的风险高于对照组,差异有统计学意义[RR=1.20,95%CI(1.02,1.42),P=0.03]。进一步研究发现,5mg/d沙格列汀单药或联合治疗致心衰的风险高于对照组,差异有统计学意义[RR=1.24,95%CI(1.05,1.46),P=0.01];2.5、10mg/d沙格列汀无论单药或联合治疗致心衰的风险与对照组比较均无显著性差异[2.5mg/d沙格列汀RR=0.69,95%CI(0.31,1.55),P=0.37;10mg/d沙格列汀RR=0.18,95%CI(0.02,1.67),P=0.13]。结论:使用5mg/d沙格列汀治疗可增加心衰风险,而其他剂量的沙格列汀均未发现增加心衰的风险。沙格列汀治疗2型糖尿病的远期安全性需进行更多大样本、高质量、长期随访的RCT加以验证

Oxidant-Free Dehydrogenation of Alcohols over Hydrotalcite-Supported Palladium Catalysts

联系人: 王 野. Tel: (0592)2186156; Fax: (0592)2183047; E-mail: [email protected][中文文摘]研究了多种载体负载Pd催化剂上苯甲醇无氧脱氢反应.结果发现,以兼具较强酸性和碱性的水滑石(HT)为载体时,Pd催化剂具有优异的苯甲醇转化活性和苯甲醛选择性,当Pd含量为0.32%～0.55%时催化性能最佳.Pd/HT催化剂可重复使用,且对于含推电子取代基的芳香醇、2-噻吩甲醇、α,β-不饱和醇与环状脂肪醇等的直接脱氢反应均具有较好催化性能.HT表面的Pd(II)物种反应后转变为平均粒径为2.0～2.5nm的Pd纳米粒子或纳米簇.具有较高分散度的Pd(II)物种易转变为较小的Pd纳米粒子,从而具有较佳的催化性能.本文推测,催化剂表面的碱性位可促进苯甲醇O-H键的活化,形成Pd-苯甲氧基中间体,该中间体进一步脱氢生成苯甲醛和Pd-H物种;而催化剂表面的质子酸位可与Pd-H作用,促进H2的脱除.[英文文摘]Pd catalysts supported on various supports have been studied for the oxidant-free dehydrogenation of alcohols.The Pd catalyst loaded on hydrotalcite (HT),which is an acid-base bifunctional support,demonstrates superior benzyl alcohol conversion and benzaldehyde selectivity.Detailed studies using the Pd/HT catalyst reveal that the best performance can be obtained over the catalyst with w(Pd)=0.32%-0.55%.The Pd/HT can be used repeatedly without significant deactivation and can efficiently catalyze the drogenation of various alcohols including the substituted benzylic alcohols, 2-thiophenemethanol, α,β-unsaturated alcohols, and cyclic aliphatic alcohols. The characterization results show that the Pd(II) species over the HT are transformed into Pd nanoparticles or nanoclusters with mean size of 2.0–2.5 nm during the reaction. The highly dispersed Pd(II) species in the catalyst precursors can lead to the formation of smaller Pd nanoparticles, favoring the catalytic performance. We speculate that the basicity of the catalyst may promote the activation of the O-H bond of alcohol to form a Pd-benzyl alcoholate intermediate, which undergoes β-H abstraction by Pd nanoparticles, and the Brönsted acid sites on the catalyst surface may facilitate the release of H2 by the interaction with the formed hydride species on Pd nanoparticles.国家自然科学基金(20873110,20625310,20923004); 国家重点基础研究发展计划(973计划,2010CB732303); 高等学校博士学科点专项科研基金(20090121110007); 福建省重大研究专项(2009HZ0002-1

基于复合型耦合器的新型透射式GT交错复用器及色散补偿

提出了基于复合型耦合器的透射式GT腔,并将其串入马赫-曾德尔干涉仪(MZI)的两臂上,得到了一种新型的交错复用器。推导了该器件的理论表达式,并进行了数值模拟。模拟结果表明,该交错复用器具有相当好的通带平坦性,大的通带带宽、截止度和阻带带宽。对该交错复用器进行了优化分析,发现腔数固定时,纹波带宽比越大(频谱越接近矩形方波),隔离度将越小。分析结果表明,可以通过增加腔的数量来解决纹波带宽与隔离度的矛盾,即交错复用器的频谱性能随着腔数的增加得以改善。相对于环形谐振腔辅助的交错复用器,该交错复用器不需要添加额外的相移器就能获得近似矩形的频谱,并且在整个工作波段范围具有很好的频谱均匀性。对于该器件的色散问题,提出了采用级联环形腔作为色散补偿器的补偿方案。模拟结果表明,级联环形腔能够很好地补偿该交错复用器的色散