Therapeutic Observation ofNa Zhi Method plus Electroacupuncture for Poststroke Uninhibited Neurogenic Bladder

目的 观察纳支法结合电针治疗中风后无抑制性神经源性膀胱的临床疗效.方法 将120例脑血管病变引起的无抑制性神经源性膀胱患者随机分为治疗组和对照组,每组60例.治疗组采用纳支法结合电针治疗;对照组采用电针疗法.每日1次,每次30 min,治疗2星期.观察两组治疗前后日排尿频率测试分度和膀胱最大容量的变化,并比较临床疗效.结果 治疗组总有效率为98.2%,愈显率为89.5%;对照组总有效率为93.1%,愈显率为72.4%.两组愈显率比较,差异有统计学意义（P〈0.05）.两组治疗前日排尿频率测试分度比较,差异无统计学意义（P〉0.05）;两组治疗前后日排尿频率测试分度比较,差异有极显著统计学意义（P〈0.01）;治疗组治疗后日排尿频率测试分度与对照组比较,差异有统计学性意义（P〈0.05）.两组治疗前膀胱最大容量比较,差异无统计学意义（P〉0.05）;两组治疗前后膀胱最大容量比较,差异有极显著统计学意义（P〈0.01）;治疗组治疗后膀胱最大容量与对照组比较差异有统计学意义（P〈0.05）.结论 电针治疗无抑制性神经源性膀胱疗效显著,结合纳支法疗效明显优于单纯电针.Objective To observe the clinical efficacy of Na Zhi method （midnight-noon ebb-flow method） plus electroacupuncture in treating poststroke uninhibited neurogenic bladder.Method A total of 120 patients with uninhibited neurogenic bladder due to cerebrovascular diseases were randomized into a treatment group and a control group, 60 cases in each group. The treatment group was intervened by Na Zhi method plus electroacupuncture; the control group was intervened by electroacupuncture. The intervention was conducted once a day, 30 min each time, for a total of 2 weeks. The daily voiding frequency index and maximum cystometric capacity （MCC） in the two groups were measured before and after the intervention, and the clinical efficacies were compared.Result The total effective rate and markedly effective rate were respectively 98.2% and 89.5% in the treatment group versus 93.1% and 72.4% in the control group. There was a significant difference in comparing the markedly effective rate between the two groups （P〈0.05）. There was no significant difference in comparing the daily voiding frequency index between the two groups before the intervention （P〉0.05）; the daily voiding frequency index was significantly changed after the intervention in both groups （P〈0.01）, and the daily voiding frequency index of the treatment group was significantly different from that of the control group after the intervention （P〈0.05）. There was no significant difference in comparing the MCC before the intervention between the two groups （P〉0.05）; the MCC was significantly changed after the intervention in both groups （P〈0.01）; the MCC of the treatment group was significantly different from that of the control group after the intervention （P〈0.05）.Conclusion Electroacupuncture can produce a significant efficacy in treating uninhibitedneurogenic bladder, while it can produce a more significant efficacy when combined withNa Zhi method

Permeation and characterization of TiO_2/PVA hybrid membranes

通过纳米TiO2粒子填充改性制备了新型TiO2/PVA杂化膜。红外光谱表明纳米TiO2表面的羟基与聚乙烯醇(PVA)链上的羟基存在较强的氢键作用。扫描电镜显示当TiO2的质量分数低于1.5%时,在PVA中分散均匀。X射线衍射显示纳米TiO2的加入降低了膜的结晶度。通过对含水质量分数低于20%的水/乙醇体系的脱水研究了该杂化膜的渗透性能,考察了TiO2粒子填充量、料液质量分数和温度与膜分离性能之间的关系。渗透通量J随着TiO2、水质量分数和温度的升高而增加,分离因子随着温度和水质量分数的升高而下降,在TiO2质量分数为1.5%时分离因子达到最佳值。40℃下分离质量分数85%的乙醇水溶液,分离因子可达1 590,渗透通量为0.049kg/(m2.h)。Novel nanometer-sized TiO2 filled polyvinyl alcohol(PVA) membranes were prepared.Fourier transform infrared(FTIR) indicates that there is a strong hydrogen-bond interaction between PVA and TiO2.Scanning electron microscope(SEM) shows that TiO2 particles are well distributed in the membrane matrix when TiO2 mass fraction is less than 1.5%.X-ray diffraction(XRD) reveals that the crystallinity of the hybrid membrane is lower than that of the PVA membrane.The permeation properties of the membrane was studied through the dehydration of water/ethanol system,of which water mass fraction is less than 20%,and the effects of TiO2 mass fraction,feed water mass fraction and temperature on the permeation properties of the membrane were investigated.The results show that the permeation flux J increases with increasing TiO2 mass fraction,feed water mass fraction and temperature.Meanwhile,the separation factor α decreases with increasing the feed water mass fraction and temperature.Separation factor arrives at an optimal value when TiO2 mass fraction is 1.5%.The separation factor and permeation flux reach 1 590 and 0.049 kg/(m2·h) respectively for the separation of mass fraction 85% ethanol/water solution at 40 ℃.国家自然科学基金资助项目(50573063);; 教育部新世纪优秀人才支持计划资助项

Preparation and permeation characteristics of novel PVA/APTEOS hybrid membranes

采用溶胶-凝胶法制备了聚乙烯醇(PVA)/γ-氨丙基三乙氧基硅氧烷(APTEOS)有机/无机杂化膜。用FTIR和XRD对杂化膜进行了表征。测定了膜在乙醇/水溶液中的溶胀行为。考察了杂化膜对85%(质量)的乙醇/水溶液的渗透蒸发分离性能。加入APTEOS降低了PVA的结晶度,有效控制了膜的溶胀,呈现出优良的分离性能。随着APTEOS含量的增加,杂化膜的选择性急剧增加,在5.0%(质量)时达到最大值;同时膜的渗透通量迅速增加。解决了PVA膜trade-off效应。Novel organic-inorganic hybrid membranes were prepared through sol-gel reaction of poly(vinyl alcohol)(PVA)with γ-aminopropyl-triethoxysilane(APTEOS).The resulting membranes were characterized with Fourier transform infrared spectroscopy and wide-angle X-ray diffraction.The swelling degree of PVA and PVA/APTEOS hybrid membranes in water/ethanol mixture was determined.The pervaporation performance was investigated by separation of water/ethanol mixture at 50℃.The effect of APTEOS content on the pervaporation performance was studied.The decrease in swelling degree with increasing APTEOS content was due to the formation of hydrogen and covalent bonds in the membrane matrix.The crystalline region of PVA decreased with increasing APTEOS content.The separation factor for water increased,and then decreased when APTEOS content was more than 5.0%(mass).The hybrid membrane containing 5.0%(mass) APTEOS exhibited the highest separation factor.However the permeation rate increased remarkably with increasing APTEOS content.The trade-off relation of the PVA membrane was solved,and the PVA/APTEOS hybrid membranes showed high pervaporation properties.国家自然科学基金项目(50573063);; 高等学校博士点专项基金(20050384013)~

Modeling and optimization of RGV system based on improved QPSO

为提高自动小车存取系统中轨道导引小车系统的出入库作业效率,提出了一种基于改进量子微粒群的优化方法。分析了轨道导引小车系统出入库作业任务队列特征,建立了数学模型。在此基础上利用量子微粒群算法进行优化调度,并在该算法中引入高斯变异算子,克服了其容易陷入局部最优的缺点。通过仿真实验表明了方法的可行性和有效性。To improve the performance of Rail-Guided Vehicles System(RGVS) in Automatic Vehicle Storage and Retrieval Systems(AVS/RS),an optimization method based on improved Quantum Particle Swarm Optimization(QPSO) was proposed.Firstly,sequencing characteristics of tasks in RGVS were analyzed,and a mathematical model was established.Then,a QPSO algorithm was proposed to solve the scheduling problem.Meanwhile,the Gaussian mutation operator was introduced into this algorithm to overcome its shortcoming of falling into local convergence.Finally,feasibility and effectiveness of the presented method was shown by experimental results.国家自然科学基金资助项目(60975052);厦门大学国家“211三期工程建设”资助项目(0630-E62000)---

Advances in inorganic particle filled polymeric membranes

[中文文摘]根据有机、无机组分间相互作用类型对有机-无机杂化膜进行了分类,重点论述了无机粒子填充型有机-无机杂化膜的最新研究进展,归纳了此类杂化膜的优异性能,总结了无机粒子的物理化学性能、含量、尺寸及其与聚合物的相容性等因素对此类杂化膜结构和性能的主要影响。最后提出了目前研究中存在的一些问题,并对其发展做出了简要的述评。[英文文摘]Hybrid organic-inorganic membranes are being classified according to the links between the organic and inorganic components ,the advances in inorganic particles filled polymeric membranes are reviewed emphatically. The superior properties of this kind of hybrid membranes are briefly discussed and some influencing factors such as physical and chemical properties ,content ,size of inorganic particles and its compatibility with polymers on the morphology and characteristics of the hybrid membranes are illustrated. Finally , some problems which existed in the present research are pointed out and a brief comment on their development is given.国家自然科学基金项目(50573063);高等学校博士点专项基金项目(20050384013

改良分子信标-实时PCR快速检测产单核李斯特菌

目的:建立改良分子信标-实时PCR检测产单核李斯特菌(LMO)的快速方法,应用于食品中LMO的污染状况调查及食物中毒快速诊断。方法:根据GenBank公布的LMO hlyA基因的保守序列,设计引物和改良分子信标探针,建立改良分子信标-实时PCR检测体系,应用于食品中LMO检测。结果:改良分子信标-实时PCR反应体系DNA灵敏度为110 fg,菌液灵敏度为99 cfu/m l或4 cfu/PCR反应体系,无交叉反应。以此反应体系检测28株LMO,均出现特异的荧光信号。上述方法可将检测时间由原来的至少4 d缩短至1 d。对228份食品进行LMO检测,8份增菌液LMO实时荧光PCR阳性,其中6份LMO细菌培养阳性。结论:改良分子信标-实时PCR反应体系快速、灵敏度高,特异性强,能提高LMO的检出率和准确性,可应用于LMO食品污染状况调查及食物中毒的快速诊断

改良分子信标-实时PCR快速检测志贺菌

目的:建立改良分子信标-实时PCR检测志贺菌的快速方法,应用于志贺菌食物中毒的快速诊断和门诊肠道致病菌的检测。方法:根据GenBank公布志贺菌ipaH基因的保守序列,设计引物和改良分子信标探针,建立实时PCR-改良分子信标检测体系,应用于对志贺菌食物中毒的快速诊断和门诊肠道致病菌的检测。结果:改良分子信标-实时PCR反应体系DNA灵敏度为93 fgμ/l,菌液灵敏度为64 cfu/m l或2 cfu/PCR反应体系,无交叉反应。此反应体系检测67株志贺菌,均出现特异的荧光信号。对细菌性食物中毒样本等共657份样品进行志贺菌检测,42份志贺菌实时PCR阳性,其中41份志贺菌细菌培养阳性。从样品处理到检测结果仅需2 h~1 d时间。结论:改良分子信标-实时PCR检测体系快速、灵敏度高,特异性强,可用于志贺菌食物中毒的快速诊断,为食源性疾病的分子流行病学调查提供新的检测手段,对于提高肠道门诊的工作效率有重要意义

暴露测年样品中26Al和10Be分离及其加速器质谱测定

在已有实验流程基础上,建立并优化了石英样品中Be和Al提取、纯化等实验流程,设计的流程条件实验包括实验试剂、器皿和离子交换柱选择、离子交换树脂分离Be和Al时酸浓度选择等。结果表明,选择钢铁研究总院研制的~9Be标准溶液作为~(10)Be样品制备的载体;使用一次性实验器皿;选用4 cm规格的离子交换柱;用0.05 mol/L草酸和0.75 mol/L盐酸混合溶液洗脱吸附于阴离子树脂上的Al,可有效提取、纯化样品中的Be和Al。加速器质谱(AMS)测量结果显示,13组化学空白的~(10)Be/~9Be和~(26)Al/~(27)Al比值平均值分别为7.48×10~(-15)和1.96×10~(-15),与国内已有宇宙成因核素实验室的结果(5×10~(-15)~8×10~(-15))具有可比性。电感耦合等离子体发射光谱(ICP-AES)的测量结果表明,Be、Al回收率分别达90%和60%。基于新建立的实验流程分析了祁连山北侧金佛寺的一个岩石样品,获得了~(10)Be和~(26)Al的暴露年代分别为(10.7±1.0)ka和(10.0±1.2)ka,与前人研究结果一致

改良分子信标实时PCR快速检测大肠杆菌O_(157):H_7

目的建立改良分子信标实时PCR快速检测大肠杆菌O157H7的快速方法。方法根据GenBank公布O157H7的rfbE保守序列,设计一对引物和改良分子信标探针,用FAM荧光剂标记探针的5’端,建立改良分子信标实时PCR检测O157H7的反应体系,应用于食物中毒快速诊断和食品微生物检测。结果共检测11种细菌,只有大肠杆菌O157H7有荧光信号,其余10种全无荧光信号,且与其他细菌无交叉反应,DNA灵敏度为64fg,菌液灵敏度为59cfu/ml或2cfu/PCR反应体系。应用改良分子信标实时PCR反应体系检测10株O157H7均出现特异的荧光信号,无干扰。对89份食品样品进行检测,5份O157H7实时PCR阳性,其余样品为阴性,检测仅需2h。5份阳性样本,经传统方法培养,有3份检出大肠杆菌O157H7。结论改良分子信标实时PCR检测体系快速、灵敏度高,特异性强,可用于大肠杆菌O157H7食物中毒的快速诊断和食品微生物检测,为食源性疾病的分子流行病学调查提供新的检测手段